Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0003920

Physical Location & Seq

Genomic Coordinates Reverse (-)

49625557 .. 49628605

Gene Structure 3049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014232

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1236 bp

ATGAGCTTCATTTTCAGTTTTATGGTATATGCAGAGCTTCGATTGATTGGATTCTACATAATGGCTGCTTCATCGTCCCTGTTTTCATGTTCGATCCAAGGGGTAATGCCGTTTCAGAAAACAAAGACAGACTGTAAACAGTTCCAGTGCAATTATTCAGATCTTGTCTGTAAGCAATTCAGTCACTCCAACTCAATGCACCTAAAACATCCAAGGTTGTTACCCAATGAGAAATTCTCACAAAGTGAGACTTTGTGTCATGCACTGAAAGTTGAAAATTCACCATCCTTTTTGGTGGGGAAGAAGTTTCAACTTGATGATGTGATTGAAGCTCAACAGTTTGATAGAGATATTCTGGGTGCTATATTTGAAGTTGCACGTGATATGGAGAAGATTGAAAAAAGATCACCAGGAAGCCAAATTCTTAAGGGATATCTCATGGCTACTCTCTTTTATGAACCCTCTACTAGAACTAGGCTGTCCTTTGAGTCTGCCATGAAACGGTTAGGTGGAGAAGTTTTGACAACTGAAAATGCAAGGGAGTTTTCTTCAGCAGCTAAAGGAGAAACACTTGAAGATAGTATAAGAACCGTTGAGGGTTACTCGGATATAATTGTGATGCGCCACTTTGAGAGTGGTGCAGCCAAAAGAGCAGCAGCAACTGCTGCCATTCCTGTAATTAATGCAGGAGATGGTCCTGGACAACACCCAACTCAGGCCCTTTTGGATGTCTATACTATTGAAAGAGAGATAGGAAAGCTGGATGGCATAAAAGTTGCACTTGTTGGAGATCTTGCCAATGGAAGGACAGTGCGTTCACTTGCATACTTGCTTGCCAGGTATAATGATGTCAAGATCTACTTCGTCTCTCCTGATGCAGTGAAAATGAAGAATGATATAAAAGATTATTTAACATCAAGGAAAGTTGAGTGGGAAGAAAGTGCTGATTTGATGGAAGTTGCATCTAAGTGTGACATTCTCTATCAAACTCGCATTCAGCGTGAGCGGTTTGGAGAAAGAATCGATCTCTATGAAGAAGCTCGAGGGAAGTACATTGTGGACCGTCATGTGTTAGAGGTGATGCAGAAGCATGCTGTGGTCATGCACCCTCTGCCTAGACTTGATGAGATTACTGTCGATGTTGATGGGGATCCGAGAGCTGCCTATTTCAGACAGGCAAAGAACGGCCTATATATTAGGATGGCCCTATTGAAACTTCTACTTGTAGGGTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004070 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006220 GO:0006221 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009165 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009267 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016036 GO:0016740 GO:0016741 GO:0016743 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034641 GO:0034654 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046390 GO:0046483 GO:0050896 GO:0051716 GO:0055086 GO:0071496 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00240 ko00250 ko01100 map00240 map00250 map01100

Pfam Domains

Protein Families

OTCace OTCace_N

Protein Analysis

412

Amino Acids

46.58

Weight (kDa)

7.11

Isoelectric Point (pI)

37.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
OTCace_N	PF02729	107 - 248	7.9e-45	Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain
OTCace	PF00185	255 - 406	1.4e-32	Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003920)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20330 AT3G20330
fragaria_vesca	FvH4_4g09330 FvH4_4g09330 FvH4_4g09330 FvH4_4g09330 FvH4_4g09330 FvH4_4g09330 FvH4_7g16631 FvH4_7g16631
malus_domestica	MD15G1397500.v1.1
prunus_persica	Prupe.4G221200_v2.0.a1
pyrus_communis	pycom15g35730
rosa_chinensis	RchiOBHm_Chr4g0402721 RchiOBHm_Chr6g0264841
rosa_laevigata	RLG00000009051 RLG00000014232
rosa_multiflora	Rmu_sc0000546.1_g000042 Rmu_sc0002451.1_g000002
rosa_roxburghii	Rroxscaffold_175G00432030 Rroxscaffold_5G00347480 Rroxscaffold_7G00203190
rosa_rugosa	Rorug04G0038000 Rorug06G0014400
rosa_samantha	Rh4AG115600 Rh4BG108100 Rh4CG122500 Rh4DG108100 Rh6AG134700 Rh6BG132300 Rh6CG130900 Rh6DG117900
rosa_wichuraiana	Rw4G009240 Rw6G011660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1006
AciI	CCGC	1 cut(s)	1006
AclWI	GGATC	3 cut(s)	88, 1145, 1158
AcsI	RAATTY	3 cut(s)	233, 277, 420
AcuI	CTGAAG	1 cut(s)	534
AcvI	CACGTG	1 cut(s)	380
AdeI	CACNNNGTG	1 cut(s)	245
AfaI	GTAC	1 cut(s)	1052
AfiI	CCNNNNNNNGG	3 cut(s)	501, 715, 804
AflII	CTTAAG	1 cut(s)	425
AgsI	TTSAA	8 cut(s)	275, 311, 329, 371, 398, 575, 743, 1213
AhdI	GACNNNNNGTC	1 cut(s)	255
AjnI	CCWGG	3 cut(s)	409, 697, 836
AjuI	GAANNNNNNNTTGG	2 cut(s)	218, 250
AloI	GAACNNNNNNTCC	2 cut(s)	799, 831
AluBI	AGCT	7 cut(s)	6, 37, 332, 557, 760, 1040, 1160
AluI	AGCT	7 cut(s)	6, 37, 332, 557, 760, 1040, 1160
Alw26I	GTCTC	2 cut(s)	242, 871
AlwI	GGATC	3 cut(s)	88, 1145, 1158
AlwNI	CAGNNNCTG	1 cut(s)	662
Ama87I	CYCGRG	1 cut(s)	1041
AoxI	GGCC	3 cut(s)	717, 1186, 1203
ApeKI	GCWGC	7 cut(s)	65, 554, 641, 653, 656, 665, 1160
ApoI	RAATTY	3 cut(s)	233, 277, 420
AseI	ATTAAT	1 cut(s)	681
AspLEI	GCGC	1 cut(s)	624
AspS9I	GGNCC	4 cut(s)	695, 718, 1060, 1204
AsuHPI	GGTGA	3 cut(s)	273, 399, 1090
AvaI	CYCGRG	1 cut(s)	1041
AvaII	GGWCC	2 cut(s)	695, 1060
BamHI	GGATCC	1 cut(s)	1150
BbrPI	CACGTG	1 cut(s)	380
BbvI	GCAGC	7 cut(s)	52, 566, 652, 653, 665, 668, 1147
BccI	CCATC	6 cut(s)	292, 686, 758, 946, 1139, 1195
BceAI	ACGGC	2 cut(s)	94, 1201
BciT130I	CCWGG	3 cut(s)	411, 699, 838
BcoDI	GTCTC	2 cut(s)	242, 871
BfaI	CTAG	3 cut(s)	468, 474, 1116
BfrI	CTTAAG	1 cut(s)	425
BglII	AGATCT	3 cut(s)	160, 790, 855
BisI	GCNGC	7 cut(s)	66, 555, 642, 654, 657, 666, 1161
BlsI	GCNGC	7 cut(s)	67, 556, 643, 655, 658, 667, 1162
Bme1390I	CCNGG	3 cut(s)	411, 699, 838
Bme18I	GGWCC	2 cut(s)	695, 1060
BmeRI	GACNNNNNGTC	1 cut(s)	255
BmeT110I	CYCGRG	1 cut(s)	1041
BmgT120I	GGNCC	4 cut(s)	695, 718, 1060, 1204
BmiI	GGNNCC	1 cut(s)	1152
BmrFI	CCNGG	3 cut(s)	411, 699, 838
BmsI	GCATC	4 cut(s)	609, 865, 971, 1071
BplI	GAGNNNNNCTC	4 cut(s)	221, 253, 587, 619
Bsa29I	ATCGAT	1 cut(s)	1023
BsaAI	YACGTR	1 cut(s)	380
BsaBI	GATNNNNATC	1 cut(s)	1149
BsaJI	CCNNGG	2 cut(s)	97, 212
Bsc4I	CCNNNNNNNGG	3 cut(s)	501, 715, 804
Bse1I	ACTGG	1 cut(s)	145
Bse8I	GATNNNNATC	1 cut(s)	1149
BseBI	CCWGG	3 cut(s)	411, 699, 838
BseCI	ATCGAT	1 cut(s)	1023
BseDI	CCNNGG	2 cut(s)	97, 212
BseGI	GGATG	5 cut(s)	208, 284, 733, 769, 1206
BseJI	GATNNNNATC	1 cut(s)	1149
BseLI	CCNNNNNNNGG	3 cut(s)	501, 715, 804
BseMII	CTCAG	1 cut(s)	728
BseNI	ACTGG	1 cut(s)	145
BseXI	GCAGC	7 cut(s)	52, 566, 652, 653, 665, 668, 1147
BsgI	GTGCAG	1 cut(s)	660
BshFI	GGCC	3 cut(s)	719, 1188, 1205
BshVI	ATCGAT	1 cut(s)	1023
BsiHKCI	CYCGRG	1 cut(s)	1041
BslFI	GGGAC	1 cut(s)	61
BslI	CCNNNNNNNGG	3 cut(s)	501, 715, 804
BsmAI	GTCTC	2 cut(s)	242, 871
BsmBI	CGTCTC	1 cut(s)	871
BsmFI	GGGAC	1 cut(s)	61
BsmI	GAATGC	1 cut(s)	993
BsnI	GGCC	3 cut(s)	719, 1188, 1205
BsoBI	CYCGRG	1 cut(s)	1041
Bsp143I	GATC	7 cut(s)	93, 160, 404, 790, 855, 1024, 1150
BspACI	CCGC	1 cut(s)	1006
BspANI	GGCC	3 cut(s)	719, 1188, 1205
BspCNI	CTCAG	1 cut(s)	727
BspDI	ATCGAT	1 cut(s)	1023
BspLI	GGNNCC	1 cut(s)	1152
BspPI	GGATC	3 cut(s)	88, 1145, 1158
BspTI	CTTAAG	1 cut(s)	425
BsrBI	CCGCTC	1 cut(s)	1006
BsrI	ACTGG	1 cut(s)	145
BssECI	CCNNGG	2 cut(s)	97, 212
BssMI	GATC	7 cut(s)	93, 160, 404, 790, 855, 1024, 1150
BssT1I	CCWWGG	2 cut(s)	97, 212
Bst2UI	CCWGG	3 cut(s)	411, 699, 838
Bst4CI	ACNGT	8 cut(s)	134, 141, 339, 504, 592, 811, 1064, 1135
BstAFI	CTTAAG	1 cut(s)	425
BstAPI	GCANNNNNTGC	3 cut(s)	662, 665, 1111
BstBAI	YACGTR	1 cut(s)	380
BstC8I	GCNNGC	2 cut(s)	834, 1092
BstDEI	CTNAG	2 cut(s)	714, 966
BstF5I	GGATG	5 cut(s)	208, 284, 733, 769, 1206
BstHHI	GCGC	1 cut(s)	624
BstKTI	GATC	7 cut(s)	96, 163, 407, 793, 858, 1027, 1153
BstMAI	GTCTC	2 cut(s)	242, 871
BstMBI	GATC	7 cut(s)	93, 160, 404, 790, 855, 1024, 1150
BstMWI	GCNNNNNNNGC	4 cut(s)	650, 662, 665, 1111
BstNI	CCWGG	3 cut(s)	411, 699, 838
BstNSI	RCATGY	1 cut(s)	1094
BstSCI	CCNGG	3 cut(s)	409, 697, 836
BstV1I	GCAGC	7 cut(s)	52, 566, 652, 653, 665, 668, 1147
BstX2I	RGATCY	4 cut(s)	160, 790, 855, 1150
BstYI	RGATCY	4 cut(s)	160, 790, 855, 1150
Bsu15I	ATCGAT	1 cut(s)	1023
BsuRI	GGCC	3 cut(s)	719, 1188, 1205
BsuTUI	ATCGAT	1 cut(s)	1023
BtsCI	GGATG	5 cut(s)	208, 284, 733, 769, 1206
BtsI	GCAGTG	1 cut(s)	885
BtsIMutI	CAGTG	4 cut(s)	152, 263, 816, 885
Cac8I	GCNNGC	2 cut(s)	834, 1092
CaiI	CAGNNNCTG	1 cut(s)	662
CfoI	GCGC	1 cut(s)	624
Cfr13I	GGNCC	4 cut(s)	695, 718, 1060, 1204
ClaI	ATCGAT	1 cut(s)	1023
Csp6I	GTAC	1 cut(s)	1051
CviAII	CATG	7 cut(s)	87, 260, 439, 496, 1067, 1091, 1102
CviQI	GTAC	1 cut(s)	1051
DdeI	CTNAG	2 cut(s)	714, 966
DpnI	GATC	7 cut(s)	95, 162, 406, 792, 857, 1026, 1152
DpnII	GATC	7 cut(s)	93, 160, 404, 790, 855, 1024, 1150
DraIII	CACNNNGTG	1 cut(s)	245
DriI	GACNNNNNGTC	1 cut(s)	255
Eam1105I	GACNNNNNGTC	1 cut(s)	255
Eco130I	CCWWGG	2 cut(s)	97, 212
Eco32I	GATATC	1 cut(s)	434
Eco47I	GGWCC	2 cut(s)	695, 1060
Eco57I	CTGAAG	1 cut(s)	534
Eco72I	CACGTG	1 cut(s)	380
Eco88I	CYCGRG	1 cut(s)	1041
EcoO109I	RGGNCCY	1 cut(s)	718
EcoRII	CCWGG	3 cut(s)	409, 697, 836
EcoRV	GATATC	1 cut(s)	434
EcoT14I	CCWWGG	2 cut(s)	97, 212
ErhI	CCWWGG	2 cut(s)	97, 212
Esp3I	CGTCTC	1 cut(s)	871
FaeI	CATG	7 cut(s)	90, 263, 442, 499, 1070, 1094, 1105
FalI	AAGNNNNNCTT	6 cut(s)	235, 267, 765, 797, 845, 877
FaqI	GGGAC	1 cut(s)	61
FatI	CATG	7 cut(s)	86, 259, 438, 495, 1066, 1090, 1101
Fnu4HI	GCNGC	7 cut(s)	66, 555, 642, 654, 657, 666, 1161
FokI	GGATG	5 cut(s)	195, 271, 740, 776, 1213
Fsp4HI	GCNGC	7 cut(s)	66, 555, 642, 654, 657, 666, 1161
FspBI	CTAG	3 cut(s)	468, 474, 1116
GlaI	GCGC	1 cut(s)	623
GluI	GCNGC	7 cut(s)	66, 555, 642, 654, 657, 666, 1161
HaeIII	GGCC	3 cut(s)	719, 1188, 1205
HhaI	GCGC	1 cut(s)	624
Hin1II	CATG	7 cut(s)	90, 263, 442, 499, 1070, 1094, 1105
Hin6I	GCGC	1 cut(s)	622
HinP1I	GCGC	1 cut(s)	622
HinfI	GANTC	3 cut(s)	51, 488, 1020
HphI	GGTGA	3 cut(s)	273, 399, 1090
Hpy166II	GTNNAC	3 cut(s)	137, 818, 1060
Hpy188I	TCNGA	5 cut(s)	117, 160, 607, 1155, 1172
Hpy188III	TCNNGA	2 cut(s)	853, 872
Hpy8I	GTNNAC	3 cut(s)	137, 818, 1060
HpyAV	CCTTC	1 cut(s)	798
HpyCH4III	ACNGT	8 cut(s)	134, 141, 339, 504, 592, 811, 1064, 1135
HpyCH4IV	ACGT	1 cut(s)	379
HpyF10VI	GCNNNNNNNGC	4 cut(s)	650, 662, 665, 1111
HpyF3I	CTNAG	2 cut(s)	714, 966
HpySE526I	ACGT	1 cut(s)	379
Hsp92II	CATG	7 cut(s)	90, 263, 442, 499, 1070, 1094, 1105
HspAI	GCGC	1 cut(s)	622
Kzo9I	GATC	7 cut(s)	93, 160, 404, 790, 855, 1024, 1150
Lsp1109I	GCAGC	7 cut(s)	52, 566, 652, 653, 665, 668, 1147
LweI	GCATC	4 cut(s)	609, 865, 971, 1071
MaeI	CTAG	3 cut(s)	468, 474, 1116
MaeII	ACGT	1 cut(s)	379
MaeIII	GTNAC	4 cut(s)	182, 219, 599, 971
MalI	GATC	7 cut(s)	95, 162, 406, 792, 857, 1026, 1152
MbiI	CCGCTC	1 cut(s)	1006
MboI	GATC	7 cut(s)	93, 160, 404, 790, 855, 1024, 1150
MboII	GAAGA	7 cut(s)	313, 403, 540, 587, 901, 947, 1046
MflI	RGATCY	4 cut(s)	160, 790, 855, 1150
MluCI	AATT	7 cut(s)	151, 176, 233, 277, 420, 612, 678
MlyI	GAGTC	1 cut(s)	497
MmeI	TCCRAC	2 cut(s)	213, 766
MnlI	CCTC	5 cut(s)	472, 589, 1037, 1069, 1119
MseI	TTAA	3 cut(s)	426, 681, 911
MslI	CAYNNNNRTG	1 cut(s)	967
MspCI	CTTAAG	1 cut(s)	425
MspR9I	CCNGG	3 cut(s)	411, 699, 838
Mva1269I	GAATGC	1 cut(s)	993
MvaI	CCWGG	3 cut(s)	411, 699, 838
MwoI	GCNNNNNNNGC	4 cut(s)	650, 662, 665, 1111
NdeII	GATC	7 cut(s)	93, 160, 404, 790, 855, 1024, 1150
NlaIII	CATG	7 cut(s)	90, 263, 442, 499, 1070, 1094, 1105
NlaIV	GGNNCC	1 cut(s)	1152
NmuCI	GTSAC	2 cut(s)	182, 971
NspI	RCATGY	1 cut(s)	1094
PaeI	GCATGC	1 cut(s)	1094
PaeR7I	CTCGAG	1 cut(s)	1041
PcsI	WCGNNNNNNNCGW	1 cut(s)	997
PctI	GAATGC	1 cut(s)	993
PfeI	GAWTC	2 cut(s)	51, 1020
PfoI	TCCNGGA	1 cut(s)	697
PkrI	GCNGC	7 cut(s)	67, 556, 643, 655, 658, 667, 1162
PleI	GAGTC	1 cut(s)	496
PmaCI	CACGTG	1 cut(s)	380
PmlI	CACGTG	1 cut(s)	380
PpsI	GAGTC	1 cut(s)	496
Ppu21I	YACGTR	1 cut(s)	380
PshBI	ATTAAT	1 cut(s)	681
Psp6I	CCWGG	3 cut(s)	409, 697, 836
PspCI	CACGTG	1 cut(s)	380
PspGI	CCWGG	3 cut(s)	409, 697, 836
PspN4I	GGNNCC	1 cut(s)	1152
PspPI	GGNCC	4 cut(s)	695, 718, 1060, 1204
PspXI	VCTCGAGB	1 cut(s)	1041
PstNI	CAGNNNCTG	1 cut(s)	662
PsuI	RGATCY	4 cut(s)	160, 790, 855, 1150
RsaI	GTAC	1 cut(s)	1052
RsaNI	GTAC	1 cut(s)	1051
RseI	CAYNNNNRTG	1 cut(s)	967
SaqAI	TTAA	3 cut(s)	426, 681, 911
SatI	GCNGC	7 cut(s)	66, 555, 642, 654, 657, 666, 1161
Sau3AI	GATC	7 cut(s)	93, 160, 404, 790, 855, 1024, 1150
Sau96I	GGNCC	4 cut(s)	695, 718, 1060, 1204
SchI	GAGTC	1 cut(s)	497
ScrFI	CCNGG	3 cut(s)	411, 699, 838
SfaNI	GCATC	4 cut(s)	609, 865, 971, 1071
Sfr274I	CTCGAG	1 cut(s)	1041
SinI	GGWCC	2 cut(s)	695, 1060
SlaI	CTCGAG	1 cut(s)	1041
SmiMI	CAYNNNNRTG	1 cut(s)	967
SmlI	CTYRAG	2 cut(s)	425, 1041
SmoI	CTYRAG	2 cut(s)	425, 1041
SphI	GCATGC	1 cut(s)	1094
Sse9I	AATT	7 cut(s)	151, 176, 233, 277, 420, 612, 678
SsiI	CCGC	1 cut(s)	1006
SspMI	CTAG	3 cut(s)	468, 474, 1116
StyD4I	CCNGG	3 cut(s)	409, 697, 836
StyI	CCWWGG	2 cut(s)	97, 212
TaaI	ACNGT	8 cut(s)	134, 141, 339, 504, 592, 811, 1064, 1135
TaiI	ACGT	1 cut(s)	382
TaqI	TCGA	5 cut(s)	40, 92, 1023, 1042, 1137
TasI	AATT	7 cut(s)	151, 176, 233, 277, 420, 612, 678
TatI	WGTACW	1 cut(s)	1050
TfiI	GAWTC	2 cut(s)	51, 1020
Tru1I	TTAA	3 cut(s)	426, 681, 911
Tru9I	TTAA	3 cut(s)	426, 681, 911
TscAI	CASTG	4 cut(s)	152, 270, 816, 885
TseFI	GTSAC	2 cut(s)	182, 971
TseI	GCWGC	7 cut(s)	65, 554, 641, 653, 656, 665, 1160
Tsp45I	GTSAC	2 cut(s)	182, 971
TspDTI	ATGAA	6 cut(s)	60, 75, 471, 512, 902, 1047
TspRI	CASTG	4 cut(s)	152, 270, 816, 885
Vha464I	CTTAAG	1 cut(s)	425
VpaK11BI	GGWCC	2 cut(s)	695, 1060
VspI	ATTAAT	1 cut(s)	681
XapI	RAATTY	3 cut(s)	233, 277, 420
XceI	RCATGY	1 cut(s)	1094
XcmI	CCANNNNNNNNNTGG	1 cut(s)	632
XhoI	CTCGAG	1 cut(s)	1041
XspI	CTAG	3 cut(s)	468, 474, 1116

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.