Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0005453

Physical Location & Seq

Genomic Coordinates Reverse (-)

56599068 .. 56600424

Gene Structure 1357 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014708

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1278 bp

ATGGATCCTGTAACTGTTGCAAGTCTACTGACTGCTTGTGCACAATCAGATGATTCCTTAAGCGGGATGCTGATTCATTTGTATGTCATAAAGCATGGACTAGAGTTTGATCTGTTCGTAAGAGATTTGGTGTCATGGAACTCAATAATTGCTGCAAATGAGCAGAATGATGAGCCGATGACTGCGCTTGGATTCTTCAACAGGATGCAACTATCCAGAGTTAAGCCTGATTGGCTAACACTAGTGAGTTTGGCTTCCATTATAGCTCAATTAAATGATGCTGAAATGAGTAGGGCTGTTCATGGATTCATTTTGAGGAGAGATTGGTTCAAGGAAGATGTTGTTATTGGAAATGCAGTTGTGGACATGTATGCAAAGTTGGGTGCTATAGATTCTGCACGTACTGTTTTTGAAGGACTTCCTGGCAAGGATGTGGTTTCATGGAATACTTTGATCACGGGTTACCCCCAAAATAGTCTTGCAAGTGAGGCGAATGAAGTGTACTGTATGATGCAAGAGTGCAAAGAAATAATTCCAAACCAAGGAACTTGGGTTAGCATTCTACCAGCATATACCCATTTAGGGGCCTTGCAACAAGGGATGAGAATTCATGGGCGGGTGATCAAGAACTGTCTCTACTTAGATGTATTTGTGGGTACCTGCTTGATTGACATGTATGGAAAATGTGGGAGACTAGATGATGCATTATCGTTATTCTACGAAGTGCCCAGAATGAGTTCAGTCACTTGGAATGCCATGATTTCCTGTCTTGGAGTTCATGGGCATGGTGGGAAAGCTGTGAAGCTATTCAAAGATATGATAGATGAGGGGGTTAAGCCAGATCATGTAACCTTTGTATCTCTAATGGCAGCTTGTAGTCATTCAGGTTTGGTCAATGAGGGTGAATTGTACTTTCATATGATGCAGAAAGAGTACGGAATCAAGCCTGGTTTCAAGCACTACGGCTGCATGGTAGATTTACTTGGCGGAGCTGGGCATTTAGACAAGGCATATAGTTTTATTAAGACTATGCCTGTACAGCCTGATGCTTCTGTTTGGGGGGCACTACTTGGTGCGTGTAGAATACATGGGAATGCTGAATTGGGGAAAATTGCTTCAGAAGGCTTGTTTGAAGTTGATTCGGAGAATGTGGGCTACTATGTTTTGTTGTCAAATATATATGCAACAACCGGGAAATGGGAAGGTGTGGATAAAGTGAGATCTATGGCTAGAAACCGCGGACTGAGGAAGACCCGGGTGGAGCTCAATAGAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

426

Amino Acids

47.02

Weight (kDa)

6.01

Isoelectric Point (pI)

31.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	143 - 190	8.8e-06	PPR repeat family
PPR_2	PF13041	246 - 293	4.6e-13	PPR repeat family
PPR	PF01535	248 - 278	3.9e-08	PPR repeat
E_motif	PF20431	363 - 417	4e-14	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005453)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G33990 AT4G33990
fragaria_vesca	FvH4_2g05910
malus_domestica	MD05G1081900.v1.1
prunus_persica	Prupe.8G126500_v2.0.a1 Prupe.8G126500_v2.0.a1 Prupe.8G126500_v2.0.a1 Prupe.8G126500_v2.0.a1 Prupe.8G126500_v2.0.a1 Prupe.8G126500_v2.0.a1 Prupe.8G126500_v2.0.a1
pyrus_communis	pycom05g07680
rosa_chinensis	RchiOBHm_Chr2g0134891 RchiOBHm_Chr6g0257591
rosa_laevigata	RLG00000014704 RLG00000014708
rosa_multiflora	Rmu_sc0004702.1_g000004
rosa_roxburghii	Rroxscaffold_7G00208820
rosa_rugosa	Rorug02G0320700 Rorug05G0570400
rosa_samantha	Rh2AG373000 Rh2CG356900 Rh6AG087200 Rh6BG080500 Rh6CG075800 Rh6DG072600
rosa_wichuraiana	Rw2G030350 Rw6G007750 Rw6G007790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	668
Acc65I	GGTACC	1 cut(s)	656
AccB1I	GGYRCC	1 cut(s)	656
AccI	GTMKAC	1 cut(s)	25
AccII	CGCG	1 cut(s)	1239
AciI	CCGC	5 cut(s)	63, 616, 987, 1237, 1239
AclWI	GGATC	1 cut(s)	12
AcsI	RAATTY	1 cut(s)	606
AcuI	CTGAAG	1 cut(s)	1101
AfaI	GTAC	6 cut(s)	403, 503, 658, 911, 935, 1038
AfiI	CCNNNNNNNGG	5 cut(s)	63, 542, 582, 583, 1197
AflII	CTTAAG	1 cut(s)	58
AflIII	ACRYGT	2 cut(s)	366, 672
AgsI	TTSAA	6 cut(s)	199, 331, 413, 811, 955, 1133
AhlI	ACTAGT	1 cut(s)	241
AjnI	CCWGG	2 cut(s)	421, 946
AjuI	GAANNNNNNNTTGG	2 cut(s)	1085, 1117
AluBI	AGCT	6 cut(s)	266, 797, 805, 872, 992, 1264
AluI	AGCT	6 cut(s)	266, 797, 805, 872, 992, 1264
Alw21I	GWGCWC	2 cut(s)	43, 1266
Alw26I	GTCTC	2 cut(s)	638, 685
Alw44I	GTGCAC	1 cut(s)	39
AlwI	GGATC	1 cut(s)	12
Ama87I	CYCGRG	1 cut(s)	1254
AoxI	GGCC	1 cut(s)	585
ApaLI	GTGCAC	1 cut(s)	39
ApeKI	GCWGC	3 cut(s)	152, 869, 966
ApoI	RAATTY	1 cut(s)	606
Asp700I	GAANNNNTTC	4 cut(s)	417, 531, 736, 806
Asp718I	GGTACC	1 cut(s)	656
AspLEI	GCGC	1 cut(s)	187
AspS9I	GGNCC	1 cut(s)	585
AsuC2I	CCSGG	3 cut(s)	1192, 1255, 1256
AsuHPI	GGTGA	2 cut(s)	631, 914
AvaI	CYCGRG	1 cut(s)	1254
BaeGI	GKGCMC	3 cut(s)	43, 729, 1066
BaeI	ACNNNNGTAYC	2 cut(s)	840, 873
BamHI	GGATCC	1 cut(s)	4
BanI	GGYRCC	1 cut(s)	656
BanII	GRGCYC	1 cut(s)	1266
BbsI	GAAGAC	1 cut(s)	1256
Bbv12I	GWGCWC	2 cut(s)	43, 1266
BbvI	GCAGC	3 cut(s)	139, 881, 953
BceAI	ACGGC	1 cut(s)	979
BciT130I	CCWGG	2 cut(s)	423, 948
BclI	TGATCA	2 cut(s)	453, 621
BcnI	CCSGG	3 cut(s)	1192, 1255, 1256
BcoDI	GTCTC	2 cut(s)	638, 685
BcuI	ACTAGT	1 cut(s)	241
BfaI	CTAG	4 cut(s)	101, 242, 695, 1230
BfmI	CTRYAG	1 cut(s)	387
BfrI	CTTAAG	1 cut(s)	58
BfuAI	ACCTGC	1 cut(s)	668
BglI	GCCNNNNNGGC	1 cut(s)	232
BglII	AGATCT	1 cut(s)	1220
BisI	GCNGC	3 cut(s)	153, 870, 967
BlsI	GCNGC	3 cut(s)	154, 871, 968
Bme1390I	CCNGG	5 cut(s)	423, 948, 1192, 1255, 1256
BmeT110I	CYCGRG	1 cut(s)	1254
BmgT120I	GGNCC	1 cut(s)	585
BmiI	GGNNCC	3 cut(s)	6, 586, 658
BmrFI	CCNGG	5 cut(s)	423, 948, 1192, 1255, 1256
BmsI	GCATC	7 cut(s)	57, 195, 268, 501, 691, 912, 1036
BpiI	GAAGAC	1 cut(s)	1256
BpuMI	CCSGG	3 cut(s)	1192, 1255, 1256
BsaAI	YACGTR	1 cut(s)	401
BsaJI	CCNNGG	3 cut(s)	541, 1237, 1254
Bsc4I	CCNNNNNNNGG	5 cut(s)	63, 542, 582, 583, 1197
BseBI	CCWGG	2 cut(s)	423, 948
BseDI	CCNNGG	3 cut(s)	541, 1237, 1254
BseGI	GGATG	4 cut(s)	72, 210, 436, 606
BseLI	CCNNNNNNNGG	5 cut(s)	63, 542, 582, 583, 1197
BseMII	CTCAG	1 cut(s)	1235
BseRI	GAGGAG	1 cut(s)	331
BseSI	GKGCMC	3 cut(s)	43, 729, 1066
BseXI	GCAGC	3 cut(s)	139, 881, 953
BseYI	CCCAGC	1 cut(s)	992
BsgI	GTGCAG	1 cut(s)	381
Bsh1236I	CGCG	1 cut(s)	1239
BshFI	GGCC	1 cut(s)	587
BshNI	GGYRCC	1 cut(s)	656
BsiHKAI	GWGCWC	2 cut(s)	43, 1266
BsiHKCI	CYCGRG	1 cut(s)	1254
BsiSI	CCGG	2 cut(s)	1191, 1255
BslI	CCNNNNNNNGG	5 cut(s)	63, 542, 582, 583, 1197
BsmAI	GTCTC	2 cut(s)	638, 685
BsmI	GAATGC	3 cut(s)	558, 757, 1099
BsnI	GGCC	1 cut(s)	587
BsoBI	CYCGRG	1 cut(s)	1254
Bsp1286I	GDGCHC	4 cut(s)	43, 729, 1066, 1266
Bsp1407I	TGTACA	1 cut(s)	1036
Bsp143I	GATC	6 cut(s)	4, 109, 453, 621, 841, 1220
BspACI	CCGC	5 cut(s)	63, 616, 987, 1237, 1239
BspANI	GGCC	1 cut(s)	587
BspCNI	CTCAG	1 cut(s)	1236
BspFNI	CGCG	1 cut(s)	1239
BspLI	GGNNCC	3 cut(s)	6, 586, 658
BspMI	ACCTGC	1 cut(s)	668
BspPI	GGATC	1 cut(s)	12
BspT107I	GGYRCC	1 cut(s)	656
BspTI	CTTAAG	1 cut(s)	58
BsrGI	TGTACA	1 cut(s)	1036
BssECI	CCNNGG	3 cut(s)	541, 1237, 1254
BssMI	GATC	6 cut(s)	4, 109, 453, 621, 841, 1220
BssT1I	CCWWGG	1 cut(s)	541
Bst2UI	CCWGG	2 cut(s)	423, 948
Bst4CI	ACNGT	4 cut(s)	16, 406, 506, 632
BstAFI	CTTAAG	1 cut(s)	58
BstAUI	TGTACA	1 cut(s)	1036
BstBAI	YACGTR	1 cut(s)	401
BstDEI	CTNAG	2 cut(s)	640, 1244
BstDSI	CCRYGG	1 cut(s)	1237
BstEII	GGTNACC	1 cut(s)	461
BstF5I	GGATG	4 cut(s)	72, 210, 436, 606
BstFNI	CGCG	1 cut(s)	1239
BstHHI	GCGC	1 cut(s)	187
BstKTI	GATC	6 cut(s)	7, 112, 456, 624, 844, 1223
BstMAI	GTCTC	2 cut(s)	638, 685
BstMBI	GATC	6 cut(s)	4, 109, 453, 621, 841, 1220
BstMWI	GCNNNNNNNGC	3 cut(s)	232, 488, 1039
BstNI	CCWGG	2 cut(s)	423, 948
BstNSI	RCATGY	2 cut(s)	370, 676
BstPI	GGTNACC	1 cut(s)	461
BstSCI	CCNGG	5 cut(s)	421, 946, 1190, 1253, 1254
BstSFI	CTRYAG	1 cut(s)	387
BstSLI	GKGCMC	3 cut(s)	43, 729, 1066
BstUI	CGCG	1 cut(s)	1239
BstV1I	GCAGC	3 cut(s)	139, 881, 953
BstV2I	GAAGAC	1 cut(s)	1256
BstX2I	RGATCY	2 cut(s)	4, 1220
BstYI	RGATCY	2 cut(s)	4, 1220
BsuRI	GGCC	1 cut(s)	587
BtgI	CCRYGG	1 cut(s)	1237
BtsCI	GGATG	4 cut(s)	72, 210, 436, 606
BveI	ACCTGC	1 cut(s)	668
CfoI	GCGC	1 cut(s)	187
Cfr13I	GGNCC	1 cut(s)	585
Cfr42I	CCGCGG	1 cut(s)	1240
Cfr9I	CCCGGG	1 cut(s)	1254
Csp6I	GTAC	6 cut(s)	402, 502, 657, 910, 934, 1037
CviQI	GTAC	6 cut(s)	402, 502, 657, 910, 934, 1037
DdeI	CTNAG	2 cut(s)	640, 1244
DpnI	GATC	6 cut(s)	6, 111, 455, 623, 843, 1222
DpnII	GATC	6 cut(s)	4, 109, 453, 621, 841, 1220
EciI	GGCGGA	1 cut(s)	1002
Ecl136II	GAGCTC	1 cut(s)	1264
Eco130I	CCWWGG	1 cut(s)	541
Eco24I	GRGCYC	1 cut(s)	1266
Eco53kI	GAGCTC	1 cut(s)	1264
Eco57I	CTGAAG	1 cut(s)	1101
Eco88I	CYCGRG	1 cut(s)	1254
Eco91I	GGTNACC	1 cut(s)	461
EcoICRI	GAGCTC	1 cut(s)	1264
EcoO109I	RGGNCCY	1 cut(s)	585
EcoO65I	GGTNACC	1 cut(s)	461
EcoRI	GAATTC	1 cut(s)	606
EcoRII	CCWGG	2 cut(s)	421, 946
EcoT14I	CCWWGG	1 cut(s)	541
EcoT22I	ATGCAT	1 cut(s)	706
EcoT38I	GRGCYC	1 cut(s)	1266
ErhI	CCWWGG	1 cut(s)	541
FauI	CCCGC	2 cut(s)	56, 609
FauNDI	CATATG	1 cut(s)	918
FbaI	TGATCA	2 cut(s)	453, 621
FblI	GTMKAC	1 cut(s)	25
Fnu4HI	GCNGC	3 cut(s)	153, 870, 967
FokI	GGATG	4 cut(s)	79, 217, 443, 613
FriOI	GRGCYC	1 cut(s)	1266
Fsp4HI	GCNGC	3 cut(s)	153, 870, 967
FspBI	CTAG	4 cut(s)	101, 242, 695, 1230
GlaI	GCGC	1 cut(s)	186
GluI	GCNGC	3 cut(s)	153, 870, 967
GsaI	CCCAGC	1 cut(s)	996
HaeIII	GGCC	1 cut(s)	587
HapII	CCGG	2 cut(s)	1191, 1255
HhaI	GCGC	1 cut(s)	187
Hin6I	GCGC	1 cut(s)	185
HinP1I	GCGC	1 cut(s)	185
HinfI	GANTC	7 cut(s)	53, 73, 192, 306, 392, 939, 1139
HpaII	CCGG	2 cut(s)	1191, 1255
HphI	GGTGA	2 cut(s)	631, 914
Hpy166II	GTNNAC	4 cut(s)	26, 41, 364, 502
Hpy188I	TCNGA	3 cut(s)	49, 1120, 1144
Hpy188III	TCNNGA	2 cut(s)	216, 625
Hpy8I	GTNNAC	4 cut(s)	26, 41, 364, 502
HpyAV	CCTTC	3 cut(s)	407, 1115, 1196
HpyCH4III	ACNGT	4 cut(s)	16, 406, 506, 632
HpyCH4IV	ACGT	1 cut(s)	400
HpyF10VI	GCNNNNNNNGC	3 cut(s)	232, 488, 1039
HpyF3I	CTNAG	2 cut(s)	640, 1244
HpySE526I	ACGT	1 cut(s)	400
HspAI	GCGC	1 cut(s)	185
KpnI	GGTACC	1 cut(s)	660
Ksp22I	TGATCA	2 cut(s)	453, 621
KspI	CCGCGG	1 cut(s)	1240
Kzo9I	GATC	6 cut(s)	4, 109, 453, 621, 841, 1220
LmnI	GCTCC	2 cut(s)	989, 1261
Lsp1109I	GCAGC	3 cut(s)	139, 881, 953
LweI	GCATC	7 cut(s)	57, 195, 268, 501, 691, 912, 1036
MaeI	CTAG	4 cut(s)	101, 242, 695, 1230
MaeII	ACGT	1 cut(s)	400
MaeIII	GTNAC	4 cut(s)	10, 461, 742, 847
MalI	GATC	6 cut(s)	6, 111, 455, 623, 843, 1222
MboI	GATC	6 cut(s)	4, 109, 453, 621, 841, 1220
MboII	GAAGA	3 cut(s)	187, 347, 1261
MflI	RGATCY	2 cut(s)	4, 1220
MhlI	GDGCHC	4 cut(s)	43, 729, 1066, 1266
MluCI	AATT	7 cut(s)	147, 269, 531, 606, 905, 1100, 1110
MnlI	CCTC	5 cut(s)	309, 481, 820, 892, 1239
Mph1103I	ATGCAT	1 cut(s)	706
MroXI	GAANNNNTTC	4 cut(s)	417, 531, 736, 806
MseI	TTAA	6 cut(s)	59, 222, 272, 834, 1023, 1276
MslI	CAYNNNNRTG	3 cut(s)	81, 783, 1092
MspA1I	CMGCKG	1 cut(s)	1239
MspCI	CTTAAG	1 cut(s)	58
MspI	CCGG	2 cut(s)	1191, 1255
MspR9I	CCNGG	5 cut(s)	423, 948, 1192, 1255, 1256
Mva1269I	GAATGC	3 cut(s)	558, 757, 1099
MvaI	CCWGG	2 cut(s)	423, 948
MvnI	CGCG	1 cut(s)	1239
MwoI	GCNNNNNNNGC	3 cut(s)	232, 488, 1039
NciI	CCSGG	3 cut(s)	1192, 1255, 1256
NdeI	CATATG	1 cut(s)	918
NdeII	GATC	6 cut(s)	4, 109, 453, 621, 841, 1220
NlaIV	GGNNCC	3 cut(s)	6, 586, 658
NmuCI	GTSAC	1 cut(s)	742
NsiI	ATGCAT	1 cut(s)	706
NspI	RCATGY	2 cut(s)	370, 676
PciI	ACATGT	2 cut(s)	366, 672
PctI	GAATGC	3 cut(s)	558, 757, 1099
PdmI	GAANNNNTTC	4 cut(s)	417, 531, 736, 806
PfeI	GAWTC	7 cut(s)	53, 73, 192, 306, 392, 939, 1139
PkrI	GCNGC	3 cut(s)	154, 871, 968
Ppu21I	YACGTR	1 cut(s)	401
PscI	ACATGT	2 cut(s)	366, 672
Psp124BI	GAGCTC	1 cut(s)	1266
Psp6I	CCWGG	2 cut(s)	421, 946
PspEI	GGTNACC	1 cut(s)	461
PspFI	CCCAGC	1 cut(s)	992
PspGI	CCWGG	2 cut(s)	421, 946
PspN4I	GGNNCC	3 cut(s)	6, 586, 658
PspPI	GGNCC	1 cut(s)	585
PsrI	GAACNNNNNNTAC	2 cut(s)	620, 652
PsuI	RGATCY	2 cut(s)	4, 1220
RsaI	GTAC	6 cut(s)	403, 503, 658, 911, 935, 1038
RsaNI	GTAC	6 cut(s)	402, 502, 657, 910, 934, 1037
RseI	CAYNNNNRTG	3 cut(s)	81, 783, 1092
SacI	GAGCTC	1 cut(s)	1266
SacII	CCGCGG	1 cut(s)	1240
SaqAI	TTAA	6 cut(s)	59, 222, 272, 834, 1023, 1276
SatI	GCNGC	3 cut(s)	153, 870, 967
Sau3AI	GATC	6 cut(s)	4, 109, 453, 621, 841, 1220
Sau96I	GGNCC	1 cut(s)	585
ScrFI	CCNGG	5 cut(s)	423, 948, 1192, 1255, 1256
SduI	GDGCHC	4 cut(s)	43, 729, 1066, 1266
SfaNI	GCATC	7 cut(s)	57, 195, 268, 501, 691, 912, 1036
SfcI	CTRYAG	1 cut(s)	387
Sfr303I	CCGCGG	1 cut(s)	1240
SgrBI	CCGCGG	1 cut(s)	1240
SmaI	CCCGGG	1 cut(s)	1256
SmiMI	CAYNNNNRTG	3 cut(s)	81, 783, 1092
SmlI	CTYRAG	1 cut(s)	58
SmoI	CTYRAG	1 cut(s)	58
SpeI	ACTAGT	1 cut(s)	241
Sse9I	AATT	7 cut(s)	147, 269, 531, 606, 905, 1100, 1110
SsiI	CCGC	5 cut(s)	63, 616, 987, 1237, 1239
SspMI	CTAG	4 cut(s)	101, 242, 695, 1230
SstI	GAGCTC	1 cut(s)	1266
StyD4I	CCNGG	5 cut(s)	421, 946, 1190, 1253, 1254
StyI	CCWWGG	1 cut(s)	541
TaaI	ACNGT	4 cut(s)	16, 406, 506, 632
TaiI	ACGT	1 cut(s)	403
TasI	AATT	7 cut(s)	147, 269, 531, 606, 905, 1100, 1110
TatI	WGTACW	3 cut(s)	501, 909, 1036
TfiI	GAWTC	7 cut(s)	53, 73, 192, 306, 392, 939, 1139
Tru1I	TTAA	6 cut(s)	59, 222, 272, 834, 1023, 1276
Tru9I	TTAA	6 cut(s)	59, 222, 272, 834, 1023, 1276
TseFI	GTSAC	1 cut(s)	742
TseI	GCWGC	3 cut(s)	152, 869, 966
Tsp45I	GTSAC	1 cut(s)	742
TspDTI	ATGAA	8 cut(s)	65, 290, 298, 429, 510, 599, 767, 905
TspGWI	ACGGA	1 cut(s)	951
TspMI	CCCGGG	1 cut(s)	1254
Vha464I	CTTAAG	1 cut(s)	58
VneI	GTGCAC	1 cut(s)	39
XapI	RAATTY	1 cut(s)	606
XceI	RCATGY	2 cut(s)	370, 676
XmaI	CCCGGG	1 cut(s)	1254
XmiI	GTMKAC	1 cut(s)	25
XmnI	GAANNNNTTC	4 cut(s)	417, 531, 736, 806
XspI	CTAG	4 cut(s)	101, 242, 695, 1230
Zsp2I	ATGCAT	1 cut(s)	706

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.