RLG00000015089

UDP-N-acetylenolpyruvoylglucosamine reductase, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017397
Physical Location & Seq
Forward (+)
61552853 .. 61554141
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015089

Sequence Viewer

Length: 897 bp
ATGGTCTCGTGCACGGAGCACTCATACTCCGTCCTCCTTCCACAATGTAAATCTCGTTCTGTTACAACTCTCCTGGACTGTTGTTGCCACATTCACCAAAAACCAGAGGCCCTTTTCTCTGCAAACAGCAGCAGCAAACAAGAACAGACCCAGAACTGGAACGAGTTGAAATTTGTTCGAGGCAAGAAGCAGTTGAAGCATCTCAGTACTTGGGGCATTGATTGCCCTTGTAATCATTTCATCCAAGTGTTCAATCAGACCCAGCTCGTTTCTGCTGTCAGATACTGTGATGAGCATTCAATCCGGTTCGTTGTCATTGGCAAAGGCTCAAATTGTTTGTTTGATGATTTGGGTTTTGATGGTTGTGTTATATTCAACCGAATCGAGTTCTTGGAAAAGAAAGAACCCGGCGCGTACACCGTTGGAAGCGGTTTTCGATTTACAGGACTTGAGTTTGCAGGAGGGATTCCTGGTTCTGTAGGAGGAGCTATTTATATGAATGCTGGAGCAAATGGGCAGGAGACCGCTGATTCTATACTTTGTGTTGATATTATAACAAAGGATGGAAAGTGTCATAGGCTTAGCAGGAGTGATCTAAATTTCAGGTATCGGTCATCTCCATTTCAATATATGCATGAATTGGCAGCTCTAAGAGAAGAGTGTCCCAACCAGTTGGGGAACAAAGTGCTGGCTTTGAATCCATCTGATATGGGAGTCGCAGCAGCTGAGTTGATAGACAAAGCTGGGTTGAAAGGTTTTAGAGTGGGAGGGGCAATGGTATCCAAAATCCATGCAAATTTTTCCATCAATTTTGGTGGTTCAACCTCTCAAGACATGCTTGAGCTCATTGCCAGAGTGAAAGGAAAAGGTTGTTCAGAAATTTGCAGTACAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_4 MurB_C

Protein Analysis

299

Amino Acids

32.64

Weight (kDa)

7.88

Isoelectric Point (pI)

36.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 80 - 195 8.5e-13 FAD binding domain
MurB_C PF02873 233 - 287 2.9e-14 UDP-N-acetylenolpyruvoylglucosamine reductase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017397)

Species Orthologous Gene IDs
prunus_persica Prupe.8G147100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252551
rosa_laevigata RLG00000015089
rosa_multiflora Rmu_sc0002016.1_g000028
rosa_roxburghii Rroxscaffold_7G00212610 Rroxscaffold_7G00212620
rosa_rugosa Rorug05G0536800
rosa_samantha Rh6AG053100 Rh6BG047000 Rh6CG045600 Rh6CG045700 Rh6DG041400
rosa_wichuraiana Rw6G004760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 554
AccII CGCG 1 cut(s) 413
AciI CCGC 2 cut(s) 429, 525
AcsI RAATTY 4 cut(s) 170, 598, 796, 879
AfaI GTAC 3 cut(s) 208, 416, 889
AfiI CCNNNNNNNGG 1 cut(s) 156
AgsI TTSAA 9 cut(s) 169, 196, 253, 300, 376, 626, 697, 751, 822
AjnI CCWGG 2 cut(s) 72, 469
AloI GAACNNNNNNTCC 2 cut(s) 457, 489
AluBI AGCT 6 cut(s) 265, 488, 647, 725, 743, 844
AluI AGCT 6 cut(s) 265, 488, 647, 725, 743, 844
Alw21I GWGCWC 3 cut(s) 14, 21, 846
Alw26I GTCTC 2 cut(s) 10, 515
Alw44I GTGCAC 1 cut(s) 10
AlwNI CAGNNNCTG 2 cut(s) 285, 725
AoxI GGCC 1 cut(s) 108
ApaLI GTGCAC 1 cut(s) 10
ApeKI GCWGC 5 cut(s) 129, 132, 644, 719, 722
ApoI RAATTY 4 cut(s) 170, 598, 796, 879
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 408
AsuHPI GGTGA 1 cut(s) 86
BaeGI GKGCMC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 846
BauI CACGAG 1 cut(s) 7
Bbv12I GWGCWC 3 cut(s) 14, 21, 846
BbvI GCAGC 5 cut(s) 141, 144, 656, 731, 734
BccI CCATC 4 cut(s) 353, 557, 709, 812
BciT130I CCWGG 2 cut(s) 74, 471
BciVI GTATCC 1 cut(s) 790
BcnI CCSGG 1 cut(s) 408
BcoDI GTCTC 2 cut(s) 10, 515
BfmI CTRYAG 1 cut(s) 477
BfuI GTATCC 1 cut(s) 790
BisI GCNGC 5 cut(s) 130, 133, 645, 720, 723
BlpI GCTNAGC 1 cut(s) 581
BlsI GCNGC 5 cut(s) 131, 134, 646, 721, 724
BmcAI AGTACT 1 cut(s) 208
Bme1390I CCNGG 3 cut(s) 74, 408, 471
BmgT120I GGNCC 1 cut(s) 109
BmrFI CCNGG 3 cut(s) 74, 408, 471
BmsI GCATC 1 cut(s) 208
BpmI CTGGAG 1 cut(s) 525
Bpu1102I GCTNAGC 1 cut(s) 581
BpuEI CTTGAG 3 cut(s) 470, 813, 860
BpuMI CCSGG 1 cut(s) 408
BsaI GGTCTC 2 cut(s) 10, 515
BsaWI WCCGGW 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 161, 670
Bse3DI GCAATG 2 cut(s) 780, 846
BseBI CCWGG 2 cut(s) 74, 471
BseGI GGATG 2 cut(s) 240, 568
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 2 cut(s) 780, 846
BseMII CTCAG 2 cut(s) 217, 717
BseNI ACTGG 2 cut(s) 161, 670
BseRI GAGGAG 1 cut(s) 498
BseSI GKGCMC 1 cut(s) 14
BseXI GCAGC 5 cut(s) 141, 144, 656, 731, 734
BseYI CCCAGC 2 cut(s) 261, 743
Bsh1236I CGCG 1 cut(s) 413
BshFI GGCC 1 cut(s) 110
BsiHKAI GWGCWC 3 cut(s) 14, 21, 846
BsiSI CCGG 2 cut(s) 304, 408
BslFI GGGAC 1 cut(s) 648
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 2 cut(s) 10, 515
BsmFI GGGAC 1 cut(s) 648
BsmI GAATGC 2 cut(s) 295, 505
BsnI GGCC 1 cut(s) 110
Bso31I GGTCTC 2 cut(s) 10, 515
Bsp1286I GDGCHC 3 cut(s) 14, 21, 846
Bsp143I GATC 1 cut(s) 592
Bsp1720I GCTNAGC 1 cut(s) 581
BspACI CCGC 2 cut(s) 429, 525
BspANI GGCC 1 cut(s) 110
BspCNI CTCAG 2 cut(s) 216, 718
BspFNI CGCG 1 cut(s) 413
BspTNI GGTCTC 2 cut(s) 10, 515
BsrDI GCAATG 2 cut(s) 780, 846
BsrI ACTGG 2 cut(s) 161, 670
BssMI GATC 1 cut(s) 592
BssSI CACGAG 1 cut(s) 7
Bst2BI CACGAG 1 cut(s) 7
Bst2UI CCWGG 2 cut(s) 74, 471
Bst4CI ACNGT 3 cut(s) 80, 287, 421
Bst6I CTCTTC 1 cut(s) 651
BstAPI GCANNNNNTGC 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 4 cut(s) 203, 581, 650, 726
BstF5I GGATG 2 cut(s) 240, 568
BstFNI CGCG 1 cut(s) 413
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 1 cut(s) 595
BstMAI GTCTC 2 cut(s) 10, 515
BstMBI GATC 1 cut(s) 592
BstMWI GCNNNNNNNGC 2 cut(s) 196, 222
BstNI CCWGG 2 cut(s) 74, 471
BstNSI RCATGY 1 cut(s) 838
BstSCI CCNGG 3 cut(s) 72, 406, 469
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 14
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 5 cut(s) 141, 144, 656, 731, 734
BstXI CCANNNNNNTGG 1 cut(s) 673
BsuI GTATCC 1 cut(s) 790
BsuRI GGCC 1 cut(s) 110
BtsCI GGATG 2 cut(s) 240, 568
Cac8I GCNNGC 1 cut(s) 690
CaiI CAGNNNCTG 2 cut(s) 285, 725
CfoI GCGC 1 cut(s) 413
Cfr13I GGNCC 1 cut(s) 109
Csp6I GTAC 3 cut(s) 207, 415, 888
CspCI CAANNNNNGTGG 2 cut(s) 796, 831
CviAII CATG 3 cut(s) 635, 791, 835
CviQI GTAC 3 cut(s) 207, 415, 888
DdeI CTNAG 4 cut(s) 203, 581, 650, 726
DpnI GATC 1 cut(s) 594
DpnII GATC 1 cut(s) 592
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Ecl136II GAGCTC 1 cut(s) 844
Eco24I GRGCYC 1 cut(s) 846
Eco31I GGTCTC 2 cut(s) 10, 515
Eco53kI GAGCTC 1 cut(s) 844
EcoICRI GAGCTC 1 cut(s) 844
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 2 cut(s) 72, 469
EcoT22I ATGCAT 1 cut(s) 636
EcoT38I GRGCYC 1 cut(s) 846
FaeI CATG 3 cut(s) 638, 794, 838
FalI AAGNNNNNCTT 2 cut(s) 822, 854
FaqI GGGAC 1 cut(s) 648
FatI CATG 3 cut(s) 634, 790, 834
Fnu4HI GCNGC 5 cut(s) 130, 133, 645, 720, 723
FokI GGATG 2 cut(s) 227, 575
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 5 cut(s) 130, 133, 645, 720, 723
GlaI GCGC 1 cut(s) 412
GluI GCNGC 5 cut(s) 130, 133, 645, 720, 723
GsaI CCCAGC 2 cut(s) 265, 747
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 1 cut(s) 110
HapII CCGG 2 cut(s) 304, 408
HhaI GCGC 1 cut(s) 413
Hin1II CATG 3 cut(s) 638, 794, 838
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HinfI GANTC 5 cut(s) 381, 466, 530, 697, 714
HpaII CCGG 2 cut(s) 304, 408
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 2 cut(s) 12, 417
Hpy188I TCNGA 4 cut(s) 258, 281, 706, 877
Hpy188III TCNNGA 1 cut(s) 830
Hpy8I GTNNAC 2 cut(s) 12, 417
HpyAV CCTTC 1 cut(s) 47
HpyCH4III ACNGT 3 cut(s) 80, 287, 421
HpyCH4V TGCA 6 cut(s) 12, 122, 458, 634, 794, 885
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 222
HpyF3I CTNAG 4 cut(s) 203, 581, 650, 726
Hsp92II CATG 3 cut(s) 638, 794, 838
HspAI GCGC 1 cut(s) 411
Kzo9I GATC 1 cut(s) 592
LmnI GCTCC 3 cut(s) 16, 485, 506
Lsp1109I GCAGC 5 cut(s) 141, 144, 656, 731, 734
LweI GCATC 1 cut(s) 208
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 594
MboI GATC 1 cut(s) 592
MboII GAAGA 1 cut(s) 668
MhlI GDGCHC 3 cut(s) 14, 21, 846
MluCI AATT 7 cut(s) 170, 331, 598, 638, 796, 808, 879
MlyI GAGTC 1 cut(s) 723
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 7 cut(s) 44, 100, 173, 455, 476, 761, 835
Mph1103I ATGCAT 1 cut(s) 636
MseI TTAA 1 cut(s) 895
MslI CAYNNNNRTG 1 cut(s) 245
MspA1I CMGCKG 2 cut(s) 527, 725
MspI CCGG 2 cut(s) 304, 408
MspR9I CCNGG 3 cut(s) 74, 408, 471
Mva1269I GAATGC 2 cut(s) 295, 505
MvaI CCWGG 2 cut(s) 74, 471
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 2 cut(s) 196, 222
NciI CCSGG 1 cut(s) 408
NdeII GATC 1 cut(s) 592
NlaIII CATG 3 cut(s) 638, 794, 838
NsiI ATGCAT 1 cut(s) 636
NspI RCATGY 1 cut(s) 838
PctI GAATGC 2 cut(s) 295, 505
PfeI GAWTC 4 cut(s) 381, 466, 530, 697
PfoI TCCNGGA 1 cut(s) 72
PkrI GCNGC 5 cut(s) 131, 134, 646, 721, 724
PleI GAGTC 1 cut(s) 722
PpsI GAGTC 1 cut(s) 722
PsiI TTATAA 1 cut(s) 554
Psp124BI GAGCTC 1 cut(s) 846
Psp6I CCWGG 2 cut(s) 72, 469
PspFI CCCAGC 2 cut(s) 261, 743
PspGI CCWGG 2 cut(s) 72, 469
PspPI GGNCC 1 cut(s) 109
PsrI GAACNNNNNNTAC 2 cut(s) 40, 72
PstNI CAGNNNCTG 2 cut(s) 285, 725
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 3 cut(s) 208, 416, 889
RsaNI GTAC 3 cut(s) 207, 415, 888
RseI CAYNNNNRTG 1 cut(s) 245
SacI GAGCTC 1 cut(s) 846
SaqAI TTAA 1 cut(s) 895
SatI GCNGC 5 cut(s) 130, 133, 645, 720, 723
Sau3AI GATC 1 cut(s) 592
Sau96I GGNCC 1 cut(s) 109
ScaI AGTACT 1 cut(s) 208
SchI GAGTC 1 cut(s) 723
ScrFI CCNGG 3 cut(s) 74, 408, 471
SduI GDGCHC 3 cut(s) 14, 21, 846
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 245
SmlI CTYRAG 3 cut(s) 449, 828, 839
SmoI CTYRAG 3 cut(s) 449, 828, 839
Sse9I AATT 7 cut(s) 170, 331, 598, 638, 796, 808, 879
SsiI CCGC 2 cut(s) 429, 525
SstI GAGCTC 1 cut(s) 846
StyD4I CCNGG 3 cut(s) 72, 406, 469
TaaI ACNGT 3 cut(s) 80, 287, 421
TaqI TCGA 3 cut(s) 178, 384, 436
TaqII GACCGA 1 cut(s) 600
TasI AATT 7 cut(s) 170, 331, 598, 638, 796, 808, 879
TatI WGTACW 2 cut(s) 206, 887
TfiI GAWTC 4 cut(s) 381, 466, 530, 697
Tru1I TTAA 1 cut(s) 895
Tru9I TTAA 1 cut(s) 895
TseI GCWGC 5 cut(s) 129, 132, 644, 719, 722
TspDTI ATGAA 3 cut(s) 229, 512, 651
TspGWI ACGGA 2 cut(s) 19, 29
VneI GTGCAC 1 cut(s) 10
XapI RAATTY 4 cut(s) 170, 598, 796, 879
XceI RCATGY 1 cut(s) 838
ZrmI AGTACT 1 cut(s) 208
Zsp2I ATGCAT 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.