Rh6AG053100

UDP-N-acetylenolpyruvoylglucosamine reductase, C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0017397
Physical Location & Seq
Reverse (-)
7817459 .. 7819146
1688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG053100.1

Sequence Viewer

Length: 963 bp
ATGAGTCGGCATTGGGCGAGTTGGGAGTTGACGAAGGACTTGGAGATGGAGAAGCAGGAGAGGAAGGCGGAGGTGGACTCGAAGGAAAGGGAGGGGTTGGAGGAGTCAGTGGCGCCGAGGAGCGTGAGGGAGGTGTTAAGGCGGGATTGGTGGCAGTGGCATGTTTCTGCTGTCAGATACTGTGATGAGCATTCAATCCGGTTCATTGTCATTGGCAAAGGCTCAAATTGTTTGTTTGATGATTTGGGTTTTGATGGTTGTGTTATACTCAGCCGAATCGAGTTCTTGGGAAAGAAAGAACCCGTCGTGTACACCGTTGGAAGCTGTTTTCGTTTTAACAAGTTGGGGGTTAAGTGCTCCAATGAAGGATTTACAGGACTTGAGTTTGCAGGAGGGATTCCTGGTTCTGTAGGAGGAGCTATTTATATGAATGCTGGAGCAAATGGGCAGGAGACCGCTGATTCTGTACATTGTGTTGATATTATAACAAAGGATGGAAAGTGTCATAGGCTTAGCAGGAGTTATCTCAATTTCGGGTATCGGTCATCTCCATTTCAAGATATGCAAGAATTGGCAGCTATAAATGCTGTTACTTTTAAACTGCAGCAATCGGAATTAGCAAAGCGAACACTATATGAGTACATGGAAAGGAGAAGAGTGTCCCAACCAGTTGAGGAACAAAGTGCTGGCTCGGTCTTTCGGAATCCATCTGATATGGGAGTCGCAGCAGCTGAGTTGATAGACAAAGCTGGGTTGAAAGGTTTTAGAGTGGGAGGGGCAATGGTATCCAAAATCCATGCAAATTTTTTCATCAATTGTGGTGGCTCAACCTCTCAAGACATGCTTGAGCTCATTGCCAGAGTGAAGGAAAAGGTTGATCAGAAATTTGCAGCACAACTTAAGGAAGAAGTAACGTATATCAATCCACATTATGGTGATGTGGACCCGGATAAGGACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_4 MurB_C

Protein Analysis

320

Amino Acids

35.95

Weight (kDa)

6.22

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 56 - 172 7.9e-16 FAD binding domain
MurB_C PF02873 210 - 307 9e-32 UDP-N-acetylenolpyruvoylglucosamine reductase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017397)

Species Orthologous Gene IDs
prunus_persica Prupe.8G147100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0252551
rosa_laevigata RLG00000015089
rosa_multiflora Rmu_sc0002016.1_g000028
rosa_roxburghii Rroxscaffold_7G00212610 Rroxscaffold_7G00212620
rosa_rugosa Rorug05G0536800
rosa_samantha Rh6AG053100 Rh6BG047000 Rh6CG045600 Rh6CG045700 Rh6DG041400
rosa_wichuraiana Rw6G004760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 485
AccB1I GGYRCC 1 cut(s) 112
AccB7I CCANNNNNTGG 1 cut(s) 932
AciI CCGC 3 cut(s) 68, 142, 456
AcsI RAATTY 2 cut(s) 802, 884
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 3 cut(s) 311, 468, 641
AfiI CCNNNNNNNGG 2 cut(s) 932, 952
AflII CTTAAG 1 cut(s) 899
AgsI TTSAA 3 cut(s) 195, 557, 757
AjnI CCWGG 1 cut(s) 400
AloI GAACNNNNNNTCC 2 cut(s) 388, 420
AluBI AGCT 6 cut(s) 324, 419, 578, 731, 749, 850
AluI AGCT 6 cut(s) 324, 419, 578, 731, 749, 850
Alw21I GWGCWC 2 cut(s) 359, 852
Alw26I GTCTC 1 cut(s) 446
AlwNI CAGNNNCTG 2 cut(s) 180, 731
ApeKI GCWGC 5 cut(s) 575, 604, 725, 728, 890
ApoI RAATTY 2 cut(s) 802, 884
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 943
AsuC2I CCSGG 1 cut(s) 947
AsuHPI GGTGA 1 cut(s) 947
AvaII GGWCC 1 cut(s) 943
BanI GGYRCC 1 cut(s) 112
BanII GRGCYC 1 cut(s) 852
Bbv12I GWGCWC 2 cut(s) 359, 852
BbvI GCAGC 5 cut(s) 587, 616, 737, 740, 902
BccI CCATC 4 cut(s) 40, 248, 488, 715
BciT130I CCWGG 1 cut(s) 402
BciVI GTATCC 1 cut(s) 796
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 1 cut(s) 947
BcoDI GTCTC 1 cut(s) 446
BfmI CTRYAG 2 cut(s) 408, 602
BfoI RGCGCY 1 cut(s) 116
BfrI CTTAAG 1 cut(s) 899
BfuI GTATCC 1 cut(s) 796
BisI GCNGC 5 cut(s) 576, 605, 726, 729, 891
BlpI GCTNAGC 1 cut(s) 512
BlsI GCNGC 5 cut(s) 577, 606, 727, 730, 892
Bme1390I CCNGG 2 cut(s) 402, 947
Bme18I GGWCC 1 cut(s) 943
BmgT120I GGNCC 1 cut(s) 943
BmiI GGNNCC 2 cut(s) 114, 945
BmrFI CCNGG 2 cut(s) 402, 947
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpmI CTGGAG 1 cut(s) 456
Bpu1102I GCTNAGC 1 cut(s) 512
BpuEI CTTGAG 3 cut(s) 401, 819, 866
BpuMI CCSGG 1 cut(s) 947
BsaHI GRCGYC 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 1 cut(s) 116
BsaWI WCCGGW 1 cut(s) 198
Bsc4I CCNNNNNNNGG 2 cut(s) 932, 952
Bse1I ACTGG 1 cut(s) 668
Bse3DI GCAATG 2 cut(s) 786, 852
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 1 cut(s) 499
BseLI CCNNNNNNNGG 2 cut(s) 932, 952
BseMI GCAATG 2 cut(s) 786, 852
BseMII CTCAG 2 cut(s) 283, 723
BseNI ACTGG 1 cut(s) 668
BseRI GAGGAG 3 cut(s) 116, 133, 429
BseXI GCAGC 5 cut(s) 587, 616, 737, 740, 902
BseYI CCCAGC 1 cut(s) 749
BshNI GGYRCC 1 cut(s) 112
BsiHKAI GWGCWC 2 cut(s) 359, 852
BsiSI CCGG 2 cut(s) 199, 947
BslFI GGGAC 1 cut(s) 646
BslI CCNNNNNNNGG 2 cut(s) 932, 952
BsmAI GTCTC 1 cut(s) 446
BsmFI GGGAC 1 cut(s) 646
BsmI GAATGC 2 cut(s) 190, 436
Bso31I GGTCTC 1 cut(s) 446
Bsp1286I GDGCHC 2 cut(s) 359, 852
Bsp1407I TGTACA 2 cut(s) 309, 466
Bsp143I GATC 1 cut(s) 877
Bsp1720I GCTNAGC 1 cut(s) 512
BspACI CCGC 3 cut(s) 68, 142, 456
BspCNI CTCAG 2 cut(s) 282, 724
BspLI GGNNCC 2 cut(s) 114, 945
BspMAI CTGCAG 1 cut(s) 606
BspT107I GGYRCC 1 cut(s) 112
BspTI CTTAAG 1 cut(s) 899
BspTNI GGTCTC 1 cut(s) 446
BsrDI GCAATG 2 cut(s) 786, 852
BsrGI TGTACA 2 cut(s) 309, 466
BsrI ACTGG 1 cut(s) 668
BssECI CCNNGG 1 cut(s) 116
BssMI GATC 1 cut(s) 877
BssNI GRCGYC 1 cut(s) 113
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 2 cut(s) 182, 316
Bst6I CTCTTC 1 cut(s) 649
BstACI GRCGYC 1 cut(s) 113
BstAFI CTTAAG 1 cut(s) 899
BstAUI TGTACA 2 cut(s) 309, 466
BstC8I GCNNGC 1 cut(s) 688
BstDEI CTNAG 3 cut(s) 269, 512, 732
BstF5I GGATG 1 cut(s) 499
BstH2I RGCGCY 1 cut(s) 116
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 1 cut(s) 880
BstMAI GTCTC 1 cut(s) 446
BstMBI GATC 1 cut(s) 877
BstMWI GCNNNNNNNGC 1 cut(s) 584
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 2 cut(s) 164, 844
BstSCI CCNGG 2 cut(s) 400, 945
BstSFI CTRYAG 2 cut(s) 408, 602
BstV1I GCAGC 5 cut(s) 587, 616, 737, 740, 902
BsuI GTATCC 1 cut(s) 796
BtsCI GGATG 1 cut(s) 499
BtsI GCAGTG 1 cut(s) 161
BtsIMutI CAGTG 2 cut(s) 114, 161
Cac8I GCNNGC 1 cut(s) 688
CaiI CAGNNNCTG 2 cut(s) 180, 731
CfoI GCGC 1 cut(s) 115
Cfr13I GGNCC 1 cut(s) 943
Csp6I GTAC 3 cut(s) 310, 467, 640
CspCI CAANNNNNGTGG 2 cut(s) 802, 837
CviAII CATG 4 cut(s) 161, 643, 797, 841
CviQI GTAC 3 cut(s) 310, 467, 640
DdeI CTNAG 3 cut(s) 269, 512, 732
DinI GGCGCC 1 cut(s) 114
DpnI GATC 1 cut(s) 879
DpnII GATC 1 cut(s) 877
DraI TTTAAA 1 cut(s) 598
Eam1104I CTCTTC 1 cut(s) 649
EarI CTCTTC 1 cut(s) 649
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 850
Eco24I GRGCYC 1 cut(s) 852
Eco31I GGTCTC 1 cut(s) 446
Eco47I GGWCC 1 cut(s) 943
Eco53kI GAGCTC 1 cut(s) 850
EcoICRI GAGCTC 1 cut(s) 850
EcoRII CCWGG 1 cut(s) 400
EcoT38I GRGCYC 1 cut(s) 852
EgeI GGCGCC 1 cut(s) 114
EheI GGCGCC 1 cut(s) 114
FaeI CATG 4 cut(s) 164, 646, 800, 844
FalI AAGNNNNNCTT 2 cut(s) 828, 860
FaqI GGGAC 1 cut(s) 646
FatI CATG 4 cut(s) 160, 642, 796, 840
FauI CCCGC 1 cut(s) 135
FbaI TGATCA 1 cut(s) 877
Fnu4HI GCNGC 5 cut(s) 576, 605, 726, 729, 891
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 1 cut(s) 852
Fsp4HI GCNGC 5 cut(s) 576, 605, 726, 729, 891
GlaI GCGC 1 cut(s) 114
GluI GCNGC 5 cut(s) 576, 605, 726, 729, 891
GsaI CCCAGC 1 cut(s) 753
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 116
HapII CCGG 2 cut(s) 199, 947
HhaI GCGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 113
Hin1II CATG 4 cut(s) 164, 646, 800, 844
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 8 cut(s) 4, 77, 104, 276, 397, 461, 703, 720
HpaII CCGG 2 cut(s) 199, 947
HphI GGTGA 1 cut(s) 947
Hpy166II GTNNAC 5 cut(s) 30, 76, 310, 312, 943
Hpy188I TCNGA 5 cut(s) 176, 613, 702, 712, 882
Hpy188III TCNNGA 2 cut(s) 557, 836
Hpy8I GTNNAC 5 cut(s) 30, 76, 310, 312, 943
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 5 cut(s) 28, 58, 76, 359, 859
HpyCH4III ACNGT 2 cut(s) 182, 316
HpyCH4IV ACGT 1 cut(s) 914
HpyCH4V TGCA 5 cut(s) 389, 565, 604, 800, 890
HpyF10VI GCNNNNNNNGC 1 cut(s) 584
HpyF3I CTNAG 3 cut(s) 269, 512, 732
HpySE526I ACGT 1 cut(s) 914
Hsp92I GRCGYC 1 cut(s) 113
Hsp92II CATG 4 cut(s) 164, 646, 800, 844
HspAI GCGC 1 cut(s) 113
KasI GGCGCC 1 cut(s) 112
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 1 cut(s) 877
LmnI GCTCC 4 cut(s) 120, 362, 416, 437
Lsp1109I GCAGC 5 cut(s) 587, 616, 737, 740, 902
MaeII ACGT 1 cut(s) 914
MaeIII GTNAC 2 cut(s) 589, 910
MalI GATC 1 cut(s) 879
MboI GATC 1 cut(s) 877
MboII GAAGA 2 cut(s) 666, 917
MfeI CAATTG 1 cut(s) 814
MhlI GDGCHC 2 cut(s) 359, 852
MluCI AATT 7 cut(s) 226, 529, 569, 614, 802, 814, 884
Mly113I GGCGCC 1 cut(s) 113
MlyI GAGTC 4 cut(s) 13, 71, 113, 729
MmeI TCCRAC 2 cut(s) 78, 298
MseI TTAA 5 cut(s) 137, 336, 351, 597, 900
MslI CAYNNNNRTG 1 cut(s) 933
MspA1I CMGCKG 2 cut(s) 458, 731
MspCI CTTAAG 1 cut(s) 899
MspI CCGG 2 cut(s) 199, 947
MspR9I CCNGG 2 cut(s) 402, 947
MunI CAATTG 1 cut(s) 814
Mva1269I GAATGC 2 cut(s) 190, 436
MvaI CCWGG 1 cut(s) 402
MwoI GCNNNNNNNGC 1 cut(s) 584
NarI GGCGCC 1 cut(s) 113
NciI CCSGG 1 cut(s) 947
NdeII GATC 1 cut(s) 877
NlaIII CATG 4 cut(s) 164, 646, 800, 844
NlaIV GGNNCC 2 cut(s) 114, 945
NmeAIII GCCGAG 1 cut(s) 141
NspI RCATGY 2 cut(s) 164, 844
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 2 cut(s) 190, 436
PfeI GAWTC 4 cut(s) 276, 397, 461, 703
PflMI CCANNNNNTGG 1 cut(s) 932
PkrI GCNGC 5 cut(s) 577, 606, 727, 730, 892
PleI GAGTC 4 cut(s) 12, 71, 112, 728
PluTI GGCGCC 1 cut(s) 116
PpsI GAGTC 4 cut(s) 12, 71, 112, 728
PsiI TTATAA 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 852
Psp6I CCWGG 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 749
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 2 cut(s) 114, 945
PspPI GGNCC 1 cut(s) 943
PstI CTGCAG 1 cut(s) 606
PstNI CAGNNNCTG 2 cut(s) 180, 731
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 3 cut(s) 311, 468, 641
RsaNI GTAC 3 cut(s) 310, 467, 640
RseI CAYNNNNRTG 1 cut(s) 933
SacI GAGCTC 1 cut(s) 852
SaqAI TTAA 5 cut(s) 137, 336, 351, 597, 900
SatI GCNGC 5 cut(s) 576, 605, 726, 729, 891
Sau3AI GATC 1 cut(s) 877
Sau96I GGNCC 1 cut(s) 943
SchI GAGTC 4 cut(s) 13, 71, 113, 729
ScrFI CCNGG 2 cut(s) 402, 947
SduI GDGCHC 2 cut(s) 359, 852
SfcI CTRYAG 2 cut(s) 408, 602
SfoI GGCGCC 1 cut(s) 114
SinI GGWCC 1 cut(s) 943
SmiMI CAYNNNNRTG 1 cut(s) 933
SmlI CTYRAG 4 cut(s) 380, 834, 845, 899
SmoI CTYRAG 4 cut(s) 380, 834, 845, 899
Sse9I AATT 7 cut(s) 226, 529, 569, 614, 802, 814, 884
SsiI CCGC 3 cut(s) 68, 142, 456
SspDI GGCGCC 1 cut(s) 112
SstI GAGCTC 1 cut(s) 852
StyD4I CCNGG 2 cut(s) 400, 945
TaaI ACNGT 2 cut(s) 182, 316
TaiI ACGT 1 cut(s) 917
TaqI TCGA 2 cut(s) 80, 279
TaqII GACCGA 2 cut(s) 531, 682
TasI AATT 7 cut(s) 226, 529, 569, 614, 802, 814, 884
TatI WGTACW 3 cut(s) 309, 466, 639
TfiI GAWTC 4 cut(s) 276, 397, 461, 703
Tru1I TTAA 5 cut(s) 137, 336, 351, 597, 900
Tru9I TTAA 5 cut(s) 137, 336, 351, 597, 900
TscAI CASTG 2 cut(s) 114, 161
TseI GCWGC 5 cut(s) 575, 604, 725, 728, 890
TspDTI ATGAA 4 cut(s) 193, 378, 443, 799
TspRI CASTG 2 cut(s) 114, 161
Van91I CCANNNNNTGG 1 cut(s) 932
Vha464I CTTAAG 1 cut(s) 899
VpaK11BI GGWCC 1 cut(s) 943
XapI RAATTY 2 cut(s) 802, 884
XceI RCATGY 2 cut(s) 164, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.