RLG00000017291

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013773
Physical Location & Seq
Forward (+)
15022276 .. 15022973
698 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017291

Sequence Viewer

Length: 498 bp
ATGGAGAAGGGACACGAGCTTATTTTCAAGTGTTCCAATCGTAGTGTATGGAAGCCTTACAAAAAGTATAAATATAGTAACCAACTTCTTGAATTGCAGAACTCTCTTCAGAATCTGCTACTTGTACTGTCAGTGCAACAAGCAAGGGATGGGAAGGTGAACTTGGTTGCAACAAGGAATATAGAGATGGTGGTGCAGCGAGTAGATGTGAATGTTGCGGCATTGAGACAACACCAAGAGATGAATGTTACGAATCAAGTAAGAAGCATAAGCAGTACCGCGATCCAGCATATTGAAAACCAAGGTGAGGTGAAGCTTCTTAAGAATAATAATGTGTCAATGGTTGTGCTGACTGCTCCTGGAGGATCTGGGAAAACCACATTGGCCACAAAGTTTTGTCAAGATAAGGAAGTTGAAGGTCTTCTGGTATTAGTAGTCTTAGCAAATAGCACTATAAGGTATTATATTGTTATAGTATTGAAAAGGACTTGGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

166

Amino Acids

18.72

Weight (kDa)

9.73

Isoelectric Point (pI)

30.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 1 - 63 1.4e-07 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 281
AciI CCGC 2 cut(s) 218, 279
AclWI GGATC 2 cut(s) 277, 373
AcoI YGGCCR 1 cut(s) 384
AcuI CTGAAG 1 cut(s) 92
AfaI GTAC 2 cut(s) 126, 277
AfiI CCNNNNNNNGG 1 cut(s) 307
AflII CTTAAG 1 cut(s) 320
AgsI TTSAA 5 cut(s) 28, 92, 296, 416, 481
AjnI CCWGG 1 cut(s) 358
AjuI GAANNNNNNNTTGG 4 cut(s) 146, 178, 365, 397
AluBI AGCT 2 cut(s) 19, 316
AluI AGCT 2 cut(s) 19, 316
Alw26I GTCTC 1 cut(s) 220
AlwI GGATC 2 cut(s) 277, 373
AlwNI CAGNNNCTG 1 cut(s) 115
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 1 cut(s) 196
Asp700I GAANNNNTTC 1 cut(s) 420
AsuHPI GGTGA 3 cut(s) 169, 317, 322
BalI TGGCCA 1 cut(s) 386
BauI CACGAG 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 413
BbvI GCAGC 1 cut(s) 208
BccI CCATC 2 cut(s) 143, 181
BciT130I CCWGG 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 220
BfrI CTTAAG 1 cut(s) 320
BisI GCNGC 2 cut(s) 197, 219
BlsI GCNGC 2 cut(s) 198, 220
Bme1390I CCNGG 1 cut(s) 360
BmrFI CCNGG 1 cut(s) 360
BpiI GAAGAC 1 cut(s) 413
BpmI CTGGAG 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 301
Bsc4I CCNNNNNNNGG 1 cut(s) 307
BseBI CCWGG 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 208
BsgI GTGCAG 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 1 cut(s) 386
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 1 cut(s) 386
Bsp143I GATC 2 cut(s) 282, 365
BspACI CCGC 2 cut(s) 218, 279
BspANI GGCC 1 cut(s) 386
BspFNI CGCG 1 cut(s) 281
BspPI GGATC 2 cut(s) 277, 373
BspTI CTTAAG 1 cut(s) 320
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 2 cut(s) 282, 365
BssSI CACGAG 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 301
Bst2BI CACGAG 1 cut(s) 14
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 1 cut(s) 111
BstAFI CTTAAG 1 cut(s) 320
BstDEI CTNAG 1 cut(s) 439
BstF5I GGATG 1 cut(s) 154
BstFNI CGCG 1 cut(s) 281
BstKTI GATC 2 cut(s) 285, 368
BstMAI GTCTC 1 cut(s) 220
BstMBI GATC 2 cut(s) 282, 365
BstNI CCWGG 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 358
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 208
BstV2I GAAGAC 1 cut(s) 413
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BsuRI GGCC 1 cut(s) 386
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 138
CaiI CAGNNNCTG 1 cut(s) 115
Csp6I GTAC 2 cut(s) 125, 276
CviJI RGCY 4 cut(s) 19, 55, 316, 386
CviKI_1 RGCY 4 cut(s) 19, 55, 316, 386
CviQI GTAC 2 cut(s) 125, 276
DdeI CTNAG 1 cut(s) 439
DpnI GATC 2 cut(s) 284, 367
DpnII GATC 2 cut(s) 282, 365
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
FaiI YATR 9 cut(s) 49, 69, 75, 182, 269, 291, 455, 465, 473
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 197, 219
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 2 cut(s) 197, 219
GluI GCNGC 2 cut(s) 197, 219
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 1 cut(s) 386
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 2 cut(s) 112, 253
HphI GGTGA 3 cut(s) 169, 317, 322
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 2 cut(s) 89, 401
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 2 cut(s) 148, 410
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4V TGCA 4 cut(s) 97, 136, 170, 196
HpyF3I CTNAG 1 cut(s) 439
Kzo9I GATC 2 cut(s) 282, 365
LmnI GCTCC 1 cut(s) 361
LpnPI CCDG 5 cut(s) 299, 345, 354, 372, 410
Lsp1109I GCAGC 1 cut(s) 208
MaeIII GTNAC 2 cut(s) 77, 247
MalI GATC 2 cut(s) 284, 367
MboI GATC 2 cut(s) 282, 365
MboII GAAGA 2 cut(s) 98, 413
MflI RGATCY 1 cut(s) 365
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 1 cut(s) 92
MluNI TGGCCA 1 cut(s) 386
MnlI CCTC 2 cut(s) 301, 356
Mox20I TGGCCA 1 cut(s) 386
MroXI GAANNNNTTC 1 cut(s) 420
MscI TGGCCA 1 cut(s) 386
MseI TTAA 2 cut(s) 321, 496
Msp20I TGGCCA 1 cut(s) 386
MspCI CTTAAG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 360
MvaI CCWGG 1 cut(s) 360
MvnI CGCG 1 cut(s) 281
NdeII GATC 2 cut(s) 282, 365
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 2 cut(s) 112, 253
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 2 cut(s) 198, 220
Psp6I CCWGG 1 cut(s) 358
PspGI CCWGG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 2 cut(s) 126, 277
RsaNI GTAC 2 cut(s) 125, 276
SaqAI TTAA 2 cut(s) 321, 496
SatI GCNGC 2 cut(s) 197, 219
Sau3AI GATC 2 cut(s) 282, 365
ScrFI CCNGG 1 cut(s) 360
SetI ASST 7 cut(s) 21, 159, 307, 312, 318, 421, 461
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 1 cut(s) 92
SsiI CCGC 2 cut(s) 218, 279
StyD4I CCNGG 1 cut(s) 358
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 1 cut(s) 129
TasI AATT 1 cut(s) 92
TatI WGTACW 1 cut(s) 124
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 2 cut(s) 112, 253
Tru1I TTAA 2 cut(s) 321, 496
Tru9I TTAA 2 cut(s) 321, 496
TscAI CASTG 1 cut(s) 138
TseI GCWGC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 257
TspRI CASTG 1 cut(s) 138
Vha464I CTTAAG 1 cut(s) 320
XmnI GAANNNNTTC 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.