Rh2AG169500

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013773
Physical Location & Seq
Forward (+)
16062085 .. 16069503
7419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG169500.1

Sequence Viewer

Length: 1032 bp
ATGGAAACCGGAAAAGAAAGAAGCAAGCAGGGTGGCTCTCTCCAGTTCAGGTGTTATCAAATGAAGAAGAAGGTTCTTCTCGAGTGGTTGGCTCGGTCGCTCCGTCAGTTTTACATGTGCCTTTTACAGTCAATGGCCTCCAACTTGGAGCTTGCTGGGTCAGTCAAAGTCAATGGATTCCCAATTGGAAACTCGGAGCCTCACATTGAACATGTCCGTCCCCCTTTACAAGCTTTTTATTCGGGTCTTTGCATCACTGGACTTCATCAATTACCAAGATCAAGAATGGCGGACCTCGCTGCAGGTGGTGCTATGGGAGTGCCGTTTACTATACTGTATGAAGTGATTAAGGAAGTGGTGATCAAGACTTCGATGTTTAGACCTCTCCTTGAAGAGCTGAAGTCAAAGATAGAAGCTTTGAATCCACTGATTGCTCAGATGGACAGCTGGAATAAGGTGTTGGATCGTCCAGAGAAGGAACTTAGAGAATTTAAAGTACAAATGGAGAAGGGCCCTGAGCTTATTACCAAGTGCGCCAATCTTAGCAAGTGGAAAAGCTACAAAAAGTATAAATACAGCAACCAACTTCTTGAATTGCAGAACTCTCTTCAGAATCTGCTGTTTATACTGCAATGGCAACAAGCAAGGGATGTGAAGGAGACCTTGGTTGGAGTAAAGAATGTAGAGACGGTGGTGCAGCGAGTGGATGTGAGTGTTGCGGAATTCAAAGAAGAAGGGATGAGTGTCATGAAGGTGACCTTGGATGAGGTAAGAAATTTAGGGACGATGGTCCGGCGGATTGATAAGAAAGGTGAGGTACAGAACCCGTTAGATTGTGAAGTACCTGAACCTCCATTGGTTACAGTTGGATTGTATGTGCCTTTGAGGGAATTGAAGATGAAGCTTCTTAAGAATGATGATAATGTATCAATGCTTGTGCTAACTGCTCCTGGAGGCTGCGGGAAAACCACATTGGCCACAAAGTTTTGTCAAGATGAGGAAGTCAAAGGTCGGACTGGCCGAGCAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

343

Amino Acids

38.82

Weight (kDa)

9.15

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 97 - 232 4e-30 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 293
AciI CCGC 4 cut(s) 290, 719, 796, 960
AclWI GGATC 1 cut(s) 471
AcoI YGGCCR 2 cut(s) 975, 1018
AcsI RAATTY 3 cut(s) 488, 722, 775
AcuI CTGAAG 2 cut(s) 419, 593
AfaI GTAC 3 cut(s) 498, 819, 843
AflII CTTAAG 1 cut(s) 908
AflIII ACRYGT 2 cut(s) 114, 211
AgsI TTSAA 6 cut(s) 209, 392, 421, 593, 727, 895
AjnI CCWGG 1 cut(s) 949
AjuI GAANNNNNNNTTGG 8 cut(s) 443, 475, 647, 679, 743, 775, 956, 988
AluBI AGCT 8 cut(s) 151, 233, 397, 416, 447, 520, 558, 904
AluI AGCT 8 cut(s) 151, 233, 397, 416, 447, 520, 558, 904
Alw26I GTCTC 2 cut(s) 653, 680
AlwI GGATC 1 cut(s) 471
AlwNI CAGNNNCTG 1 cut(s) 616
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 4 cut(s) 135, 511, 975, 1018
ApaI GGGCCC 1 cut(s) 515
ApeKI GCWGC 4 cut(s) 299, 697, 957, 1025
ApoI RAATTY 3 cut(s) 488, 722, 775
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 4 cut(s) 292, 511, 512, 790
AsuHPI GGTGA 3 cut(s) 370, 766, 824
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 2 cut(s) 292, 790
BaeGI GKGCMC 1 cut(s) 515
BalI TGGCCA 1 cut(s) 977
BanII GRGCYC 1 cut(s) 515
BbvI GCAGC 3 cut(s) 286, 709, 944
BccI CCATC 2 cut(s) 433, 781
BceAI ACGGC 1 cut(s) 307
BciT130I CCWGG 1 cut(s) 951
BclI TGATCA 1 cut(s) 360
BcoDI GTCTC 2 cut(s) 653, 680
BfmI CTRYAG 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 908
BfuAI ACCTGC 1 cut(s) 293
BisI GCNGC 4 cut(s) 300, 698, 958, 1026
BlsI GCNGC 4 cut(s) 301, 699, 959, 1027
Bme1390I CCNGG 1 cut(s) 951
Bme18I GGWCC 2 cut(s) 292, 790
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 4 cut(s) 292, 511, 512, 790
BmiI GGNNCC 2 cut(s) 198, 513
BmrFI CCNGG 1 cut(s) 951
BmsI GCATC 1 cut(s) 261
BoxI GACNNNNGTC 1 cut(s) 788
BpmI CTGGAG 2 cut(s) 26, 972
Bpu10I CCTNAGC 1 cut(s) 516
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 2 cut(s) 663, 759
BsaWI WCCGGW 1 cut(s) 8
Bse1I ACTGG 3 cut(s) 43, 262, 1021
Bse3DI GCAATG 1 cut(s) 638
BseBI CCWGG 1 cut(s) 951
BseDI CCNNGG 2 cut(s) 663, 759
BseGI GGATG 4 cut(s) 655, 712, 744, 769
BseMI GCAATG 1 cut(s) 638
BseMII CTCAG 2 cut(s) 449, 507
BseNI ACTGG 3 cut(s) 43, 262, 1021
BseSI GKGCMC 1 cut(s) 515
BseXI GCAGC 3 cut(s) 286, 709, 944
BseYI CCCAGC 1 cut(s) 155
BsgI GTGCAG 1 cut(s) 716
Bsh1285I CGRYCG 1 cut(s) 98
BshFI GGCC 4 cut(s) 137, 513, 977, 1020
BsiEI CGRYCG 1 cut(s) 98
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 2 cut(s) 9, 793
BslFI GGGAC 2 cut(s) 204, 796
BsmAI GTCTC 2 cut(s) 653, 680
BsmBI CGTCTC 1 cut(s) 680
BsmFI GGGAC 2 cut(s) 204, 796
BsnI GGCC 4 cut(s) 137, 513, 977, 1020
Bso31I GGTCTC 1 cut(s) 653
BsoBI CYCGRG 1 cut(s) 80
Bsp120I GGGCCC 1 cut(s) 511
Bsp1286I GDGCHC 1 cut(s) 515
Bsp143I GATC 3 cut(s) 278, 360, 463
BspACI CCGC 4 cut(s) 290, 719, 796, 960
BspANI GGCC 4 cut(s) 137, 513, 977, 1020
BspCNI CTCAG 2 cut(s) 448, 508
BspHI TCATGA 1 cut(s) 747
BspLI GGNNCC 2 cut(s) 198, 513
BspMAI CTGCAG 1 cut(s) 304
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 1 cut(s) 471
BspQI GCTCTTC 1 cut(s) 387
BspTI CTTAAG 1 cut(s) 908
BspTNI GGTCTC 1 cut(s) 653
BsrDI GCAATG 1 cut(s) 638
BsrI ACTGG 3 cut(s) 43, 262, 1021
BssECI CCNNGG 2 cut(s) 663, 759
BssMI GATC 3 cut(s) 278, 360, 463
BssT1I CCWWGG 2 cut(s) 663, 759
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 4 cut(s) 129, 336, 691, 865
Bst6I CTCTTC 2 cut(s) 387, 612
BstAFI CTTAAG 1 cut(s) 908
BstAPI GCANNNNNTGC 1 cut(s) 308
BstC8I GCNNGC 2 cut(s) 26, 153
BstDEI CTNAG 4 cut(s) 435, 482, 516, 542
BstEII GGTNACC 1 cut(s) 754
BstF5I GGATG 4 cut(s) 655, 712, 744, 769
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 3 cut(s) 281, 363, 466
BstMAI GTCTC 2 cut(s) 653, 680
BstMBI GATC 3 cut(s) 278, 360, 463
BstMCI CGRYCG 1 cut(s) 98
BstMWI GCNNNNNNNGC 2 cut(s) 296, 308
BstNI CCWGG 1 cut(s) 951
BstNSI RCATGY 2 cut(s) 118, 215
BstPAI GACNNNNGTC 1 cut(s) 788
BstPI GGTNACC 1 cut(s) 754
BstSCI CCNGG 1 cut(s) 949
BstSFI CTRYAG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 515
BstV1I GCAGC 3 cut(s) 286, 709, 944
BsuRI GGCC 4 cut(s) 137, 513, 977, 1020
BtsCI GGATG 4 cut(s) 655, 712, 744, 769
BtsIMutI CAGTG 2 cut(s) 255, 425
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 2 cut(s) 26, 153
CaiI CAGNNNCTG 1 cut(s) 616
CciI TCATGA 1 cut(s) 747
CfoI GCGC 1 cut(s) 536
Cfr13I GGNCC 4 cut(s) 292, 511, 512, 790
Csp6I GTAC 3 cut(s) 497, 818, 842
CspCI CAANNNNNGTGG 2 cut(s) 13, 48
CviAII CATG 3 cut(s) 115, 212, 748
CviQI GTAC 3 cut(s) 497, 818, 842
DdeI CTNAG 4 cut(s) 435, 482, 516, 542
DpnI GATC 3 cut(s) 280, 362, 465
DpnII GATC 3 cut(s) 278, 360, 463
DraI TTTAAA 1 cut(s) 493
EaeI YGGCCR 2 cut(s) 975, 1018
Eam1104I CTCTTC 2 cut(s) 387, 612
EarI CTCTTC 2 cut(s) 387, 612
EciI GGCGGA 2 cut(s) 305, 811
Eco130I CCWWGG 2 cut(s) 663, 759
Eco24I GRGCYC 1 cut(s) 515
Eco31I GGTCTC 1 cut(s) 653
Eco47I GGWCC 2 cut(s) 292, 790
Eco57I CTGAAG 2 cut(s) 419, 593
Eco88I CYCGRG 1 cut(s) 80
Eco91I GGTNACC 1 cut(s) 754
EcoO109I RGGNCCY 2 cut(s) 511, 512
EcoO65I GGTNACC 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 722
EcoRII CCWGG 1 cut(s) 949
EcoT14I CCWWGG 2 cut(s) 663, 759
EcoT38I GRGCYC 1 cut(s) 515
ErhI CCWWGG 2 cut(s) 663, 759
Esp3I CGTCTC 1 cut(s) 680
FaeI CATG 3 cut(s) 118, 215, 751
FaiI YATR 9 cut(s) 116, 213, 314, 332, 339, 570, 626, 749, 876
FalI AAGNNNNNCTT 4 cut(s) 647, 679, 743, 775
FaqI GGGAC 2 cut(s) 204, 796
FatI CATG 3 cut(s) 114, 211, 747
FauI CCCGC 1 cut(s) 953
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 4 cut(s) 300, 698, 958, 1026
FokI GGATG 4 cut(s) 662, 719, 751, 776
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 4 cut(s) 300, 698, 958, 1026
GlaI GCGC 1 cut(s) 535
GluI GCNGC 4 cut(s) 300, 698, 958, 1026
GsaI CCCAGC 1 cut(s) 159
GsuI CTGGAG 2 cut(s) 26, 972
HaeIII GGCC 4 cut(s) 137, 513, 977, 1020
HapII CCGG 2 cut(s) 9, 793
HhaI GCGC 1 cut(s) 536
Hin1II CATG 3 cut(s) 118, 215, 751
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HindIII AAGCTT 3 cut(s) 231, 414, 902
HinfI GANTC 3 cut(s) 177, 421, 613
HpaII CCGG 2 cut(s) 9, 793
HphI GGTGA 3 cut(s) 370, 766, 824
Hpy166II GTNNAC 1 cut(s) 327
Hpy188I TCNGA 4 cut(s) 196, 438, 612, 1014
Hpy188III TCNNGA 7 cut(s) 80, 282, 364, 470, 590, 748, 992
Hpy8I GTNNAC 1 cut(s) 327
HpyAV CCTTC 6 cut(s) 64, 469, 502, 649, 728, 745
HpyCH4III ACNGT 4 cut(s) 129, 336, 691, 865
HpyCH4V TGCA 5 cut(s) 252, 302, 598, 631, 697
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 308
HpyF3I CTNAG 4 cut(s) 435, 482, 516, 542
Hsp92II CATG 3 cut(s) 118, 215, 751
HspAI GCGC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 3 cut(s) 278, 360, 463
LguI GCTCTTC 1 cut(s) 387
LmnI GCTCC 4 cut(s) 105, 148, 196, 952
Lsp1109I GCAGC 3 cut(s) 286, 709, 944
LweI GCATC 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 754, 859
MalI GATC 3 cut(s) 280, 362, 465
MboI GATC 3 cut(s) 278, 360, 463
MboII GAAGA 7 cut(s) 68, 76, 79, 404, 599, 743, 907
MfeI CAATTG 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 515
MlsI TGGCCA 1 cut(s) 977
MluCI AATT 7 cut(s) 183, 269, 488, 593, 722, 775, 890
MluNI TGGCCA 1 cut(s) 977
MmeI TCCRAC 5 cut(s) 165, 441, 649, 847, 992
Mox20I TGGCCA 1 cut(s) 977
MscI TGGCCA 1 cut(s) 977
MseI TTAA 3 cut(s) 348, 492, 909
MslI CAYNNNNRTG 1 cut(s) 752
Msp20I TGGCCA 1 cut(s) 977
MspA1I CMGCKG 1 cut(s) 447
MspCI CTTAAG 1 cut(s) 908
MspI CCGG 2 cut(s) 9, 793
MspR9I CCNGG 1 cut(s) 951
MunI CAATTG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 951
MwoI GCNNNNNNNGC 2 cut(s) 296, 308
NdeII GATC 3 cut(s) 278, 360, 463
NlaIII CATG 3 cut(s) 118, 215, 751
NlaIV GGNNCC 2 cut(s) 198, 513
NmuCI GTSAC 1 cut(s) 754
NspI RCATGY 2 cut(s) 118, 215
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 1 cut(s) 747
PaqCI CACCTGC 1 cut(s) 293
PciI ACATGT 2 cut(s) 114, 211
PciSI GCTCTTC 1 cut(s) 387
PcsI WCGNNNNNNNCGW 2 cut(s) 100, 1018
PfeI GAWTC 3 cut(s) 177, 421, 613
PfoI TCCNGGA 1 cut(s) 949
PkrI GCNGC 4 cut(s) 301, 699, 959, 1027
PscI ACATGT 2 cut(s) 114, 211
PshAI GACNNNNGTC 1 cut(s) 788
Psp6I CCWGG 1 cut(s) 949
PspEI GGTNACC 1 cut(s) 754
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 2 cut(s) 198, 513
PspOMI GGGCCC 1 cut(s) 511
PspPI GGNCC 4 cut(s) 292, 511, 512, 790
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 616
PvuII CAGCTG 1 cut(s) 447
RsaI GTAC 3 cut(s) 498, 819, 843
RsaNI GTAC 3 cut(s) 497, 818, 842
RseI CAYNNNNRTG 1 cut(s) 752
SapI GCTCTTC 1 cut(s) 387
SaqAI TTAA 3 cut(s) 348, 492, 909
SatI GCNGC 4 cut(s) 300, 698, 958, 1026
Sau3AI GATC 3 cut(s) 278, 360, 463
Sau96I GGNCC 4 cut(s) 292, 511, 512, 790
ScrFI CCNGG 1 cut(s) 951
SduI GDGCHC 1 cut(s) 515
SfaNI GCATC 1 cut(s) 261
SfcI CTRYAG 1 cut(s) 300
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 2 cut(s) 292, 790
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 1 cut(s) 752
SmlI CTYRAG 2 cut(s) 80, 908
SmoI CTYRAG 2 cut(s) 80, 908
Sse9I AATT 7 cut(s) 183, 269, 488, 593, 722, 775, 890
SsiI CCGC 4 cut(s) 290, 719, 796, 960
StyD4I CCNGG 1 cut(s) 949
StyI CCWWGG 2 cut(s) 663, 759
TaaI ACNGT 4 cut(s) 129, 336, 691, 865
TaqI TCGA 2 cut(s) 81, 371
TaqII GACCGA 1 cut(s) 84
TasI AATT 7 cut(s) 183, 269, 488, 593, 722, 775, 890
TatI WGTACW 1 cut(s) 496
TfiI GAWTC 3 cut(s) 177, 421, 613
Tru1I TTAA 3 cut(s) 348, 492, 909
Tru9I TTAA 3 cut(s) 348, 492, 909
TscAI CASTG 2 cut(s) 262, 432
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 4 cut(s) 299, 697, 957, 1025
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 5 cut(s) 77, 254, 354, 764, 914
TspGWI ACGGA 2 cut(s) 92, 206
TspRI CASTG 2 cut(s) 262, 432
Vha464I CTTAAG 1 cut(s) 908
VpaK11BI GGWCC 2 cut(s) 292, 790
XapI RAATTY 3 cut(s) 488, 722, 775
XceI RCATGY 2 cut(s) 118, 215
XhoI CTCGAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.