Rh2AG169000

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013773
Physical Location & Seq
Forward (+)
16015960 .. 16019795
3836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG169000.1

Sequence Viewer

Length: 756 bp
ATGGCGGCGAACCTCGCTGCAGGTGGTGCTATGGGAGTACCATTTAATGCGCTCTATGAAGCCATTAAGCAAGTGGTGGTCAAGACTAGAATGTTTAAACCCCTTGTTGAAGACCTGAGGTCGAAGATAAACGCCTTGAAACCCCTCATCGATGCAATGGAGATCTGCAATATAGCTCTGGGGATTTCAGAGGACGAACTTAGAGATTTCAAAATGCAAATGGAGAAGGGACCCAAGCTTATTCTCAAGTGTTCCAATCTTAGAGTATGGAAGACTTACAAAAAGTATAAATACAGTAACCAACTTCTTGAATTGCAGAACTCTCTTCAGAATCTGCTACTTGTACTGTCAGTGCAACAAGCAAGGGATTTGAAGGTGAACTTGGTTGCAACAAGGAATATAGAGACGGTGGTGCAGCGGGTGGATGTGAATGTTGCGGCATTGAGACAACACCAAGAGATGAATGTTACGGATCAATTAAGAAGCATAAGCAGTACCGCGATCCAGCATATTGAAAACCAAGGTGAGGTACAATTTCGAATAGATTGTGAAGCACCTGAACCGCCACGGGTTACAGTTGGATTGGAAGGGCCTTTGATGGAATTGAAGATGAAGCTTCTTAAGAATAATAATGTGTCAATGGTTGTGCTGACTGCTCCCGGAGGATCCGGGAAAACCACATTGGCCACAAAGTTTTGTCAAGATGAGGAAGTGAAAGGTGTGCTCTACACTGGGTGGACTTACTTGGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

251

Amino Acids

28.15

Weight (kDa)

8.94

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 136 1.6e-24 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 11
Acc36I ACCTGC 1 cut(s) 11
AccII CGCG 1 cut(s) 500
AciI CCGC 5 cut(s) 5, 418, 437, 498, 563
AclWI GGATC 4 cut(s) 480, 496, 660, 673
AcoI YGGCCR 1 cut(s) 684
AcuI CTGAAG 1 cut(s) 311
AdeI CACNNNGTG 1 cut(s) 735
AfaI GTAC 4 cut(s) 39, 345, 496, 531
AfiI CCNNNNNNNGG 1 cut(s) 526
AflII CTTAAG 1 cut(s) 620
AgsI TTSAA 7 cut(s) 110, 139, 211, 311, 373, 515, 607
AhdI GACNNNNNGTC 1 cut(s) 118
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 665, 697
AluBI AGCT 3 cut(s) 176, 238, 616
AluI AGCT 3 cut(s) 176, 238, 616
Alw21I GWGCWC 1 cut(s) 726
Alw26I GTCTC 2 cut(s) 398, 439
AlwI GGATC 4 cut(s) 480, 496, 660, 673
AlwNI CAGNNNCTG 1 cut(s) 334
AoxI GGCC 2 cut(s) 590, 684
ApeKI GCWGC 2 cut(s) 17, 415
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 2 cut(s) 230, 590
AsuC2I CCSGG 2 cut(s) 660, 670
AsuHPI GGTGA 2 cut(s) 388, 536
AsuII TTCGAA 1 cut(s) 538
AvaII GGWCC 1 cut(s) 230
AxyI CCTNAGG 1 cut(s) 116
BalI TGGCCA 1 cut(s) 686
BamHI GGATCC 1 cut(s) 665
BbsI GAAGAC 2 cut(s) 117, 278
Bbv12I GWGCWC 1 cut(s) 726
BbvI GCAGC 2 cut(s) 4, 427
BccI CCATC 1 cut(s) 592
BcnI CCSGG 2 cut(s) 660, 670
BcoDI GTCTC 2 cut(s) 398, 439
BfaI CTAG 1 cut(s) 87
BfmI CTRYAG 1 cut(s) 18
BfrI CTTAAG 1 cut(s) 620
BfuAI ACCTGC 1 cut(s) 11
BglII AGATCT 1 cut(s) 162
BisI GCNGC 4 cut(s) 6, 18, 416, 438
BlsI GCNGC 4 cut(s) 7, 19, 417, 439
Bme1390I CCNGG 2 cut(s) 660, 670
Bme18I GGWCC 1 cut(s) 230
BmeRI GACNNNNNGTC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 230, 590
BmiI GGNNCC 3 cut(s) 231, 232, 667
BmrFI CCNGG 2 cut(s) 660, 670
BmrI ACTGGG 1 cut(s) 741
BmsI GCATC 1 cut(s) 142
BmuI ACTGGG 1 cut(s) 741
BpiI GAAGAC 2 cut(s) 117, 278
Bpu14I TTCGAA 1 cut(s) 538
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 2 cut(s) 660, 670
Bsa29I ATCGAT 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 520, 566
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 736
Bse21I CCTNAGG 1 cut(s) 116
Bse3DI GCAATG 1 cut(s) 162
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 2 cut(s) 520, 566
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 107
BseNI ACTGG 1 cut(s) 736
BseXI GCAGC 2 cut(s) 4, 427
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 1 cut(s) 500
BshFI GGCC 2 cut(s) 592, 686
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 726
BsiSI CCGG 2 cut(s) 660, 669
BslFI GGGAC 1 cut(s) 243
BslI CCNNNNNNNGG 1 cut(s) 526
BsmAI GTCTC 2 cut(s) 398, 439
BsmBI CGTCTC 1 cut(s) 398
BsmFI GGGAC 1 cut(s) 243
BsnI GGCC 2 cut(s) 592, 686
Bsp119I TTCGAA 1 cut(s) 538
Bsp1286I GDGCHC 1 cut(s) 726
Bsp143I GATC 4 cut(s) 162, 472, 501, 665
BspACI CCGC 5 cut(s) 5, 418, 437, 498, 563
BspANI GGCC 2 cut(s) 592, 686
BspCNI CTCAG 1 cut(s) 108
BspDI ATCGAT 1 cut(s) 150
BspFNI CGCG 1 cut(s) 500
BspLI GGNNCC 3 cut(s) 231, 232, 667
BspMAI CTGCAG 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 11
BspPI GGATC 4 cut(s) 480, 496, 660, 673
BspT104I TTCGAA 1 cut(s) 538
BspTI CTTAAG 1 cut(s) 620
BsrDI GCAATG 1 cut(s) 162
BsrI ACTGG 1 cut(s) 736
BssECI CCNNGG 2 cut(s) 520, 566
BssMI GATC 4 cut(s) 162, 472, 501, 665
BssT1I CCWWGG 1 cut(s) 520
Bst4CI ACNGT 4 cut(s) 296, 348, 409, 577
Bst6I CTCTTC 1 cut(s) 330
BstAFI CTTAAG 1 cut(s) 620
BstAPI GCANNNNNTGC 1 cut(s) 26
BstBI TTCGAA 1 cut(s) 538
BstDEI CTNAG 3 cut(s) 116, 200, 260
BstDSI CCRYGG 1 cut(s) 566
BstF5I GGATG 1 cut(s) 430
BstFNI CGCG 1 cut(s) 500
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 4 cut(s) 165, 475, 504, 668
BstMAI GTCTC 2 cut(s) 398, 439
BstMBI GATC 4 cut(s) 162, 472, 501, 665
BstMWI GCNNNNNNNGC 2 cut(s) 14, 26
BstSCI CCNGG 2 cut(s) 658, 668
BstSFI CTRYAG 1 cut(s) 18
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 2 cut(s) 4, 427
BstV2I GAAGAC 2 cut(s) 117, 278
BstX2I RGATCY 2 cut(s) 162, 665
BstYI RGATCY 2 cut(s) 162, 665
Bsu15I ATCGAT 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 116
BsuRI GGCC 2 cut(s) 592, 686
BsuTUI ATCGAT 1 cut(s) 150
BtgI CCRYGG 1 cut(s) 566
BtsCI GGATG 1 cut(s) 430
BtsIMutI CAGTG 2 cut(s) 357, 729
BveI ACCTGC 1 cut(s) 11
CaiI CAGNNNCTG 1 cut(s) 334
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 2 cut(s) 230, 590
ClaI ATCGAT 1 cut(s) 150
Csp6I GTAC 4 cut(s) 38, 344, 495, 530
CviJI RGCY 6 cut(s) 62, 176, 238, 592, 616, 686
CviKI_1 RGCY 6 cut(s) 62, 176, 238, 592, 616, 686
CviQI GTAC 4 cut(s) 38, 344, 495, 530
DdeI CTNAG 3 cut(s) 116, 200, 260
DpnI GATC 4 cut(s) 164, 474, 503, 667
DpnII GATC 4 cut(s) 162, 472, 501, 665
DraI TTTAAA 1 cut(s) 97
DraIII CACNNNGTG 1 cut(s) 735
DriI GACNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 330
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 311
Eco81I CCTNAGG 1 cut(s) 116
EcoO109I RGGNCCY 2 cut(s) 230, 590
EcoT14I CCWWGG 1 cut(s) 520
ErhI CCWWGG 1 cut(s) 520
Esp3I CGTCTC 1 cut(s) 398
FaiI YATR 8 cut(s) 32, 57, 173, 268, 288, 401, 488, 510
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 1 cut(s) 243
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 4 cut(s) 6, 18, 416, 438
FokI GGATG 1 cut(s) 437
Fsp4HI GCNGC 4 cut(s) 6, 18, 416, 438
FspBI CTAG 1 cut(s) 87
GlaI GCGC 1 cut(s) 51
GluI GCNGC 4 cut(s) 6, 18, 416, 438
HaeIII GGCC 2 cut(s) 592, 686
HapII CCGG 2 cut(s) 660, 669
HhaI GCGC 1 cut(s) 52
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HindIII AAGCTT 2 cut(s) 236, 614
HinfI GANTC 1 cut(s) 331
HpaII CCGG 2 cut(s) 660, 669
HphI GGTGA 2 cut(s) 388, 536
Hpy166II GTNNAC 2 cut(s) 379, 738
Hpy188I TCNGA 2 cut(s) 190, 330
Hpy188III TCNNGA 3 cut(s) 82, 308, 701
Hpy8I GTNNAC 2 cut(s) 379, 738
HpyAV CCTTC 3 cut(s) 220, 367, 581
HpyCH4III ACNGT 4 cut(s) 296, 348, 409, 577
HpyCH4V TGCA 8 cut(s) 20, 155, 168, 217, 316, 355, 389, 415
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 26
HpyF3I CTNAG 3 cut(s) 116, 200, 260
HspAI GCGC 1 cut(s) 50
KflI GGGWCCC 1 cut(s) 230
Kzo9I GATC 4 cut(s) 162, 472, 501, 665
LmnI GCTCC 1 cut(s) 661
LpnPI CCDG 8 cut(s) 6, 128, 164, 518, 570, 673, 682, 717
Lsp1109I GCAGC 2 cut(s) 4, 427
LweI GCATC 1 cut(s) 142
MaeI CTAG 1 cut(s) 87
MaeIII GTNAC 3 cut(s) 296, 466, 571
MalI GATC 4 cut(s) 164, 474, 503, 667
MboI GATC 4 cut(s) 162, 472, 501, 665
MboII GAAGA 5 cut(s) 122, 136, 283, 317, 619
MflI RGATCY 2 cut(s) 162, 665
MhlI GDGCHC 1 cut(s) 726
MlsI TGGCCA 1 cut(s) 686
MluCI AATT 4 cut(s) 311, 476, 533, 602
MluNI TGGCCA 1 cut(s) 686
MmeI TCCRAC 1 cut(s) 559
MnlI CCTC 7 cut(s) 23, 111, 155, 184, 520, 656, 700
Mox20I TGGCCA 1 cut(s) 686
MscI TGGCCA 1 cut(s) 686
MseI TTAA 5 cut(s) 45, 66, 96, 479, 621
Msp20I TGGCCA 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 418
MspCI CTTAAG 1 cut(s) 620
MspI CCGG 2 cut(s) 660, 669
MspR9I CCNGG 2 cut(s) 660, 670
MssI GTTTAAAC 1 cut(s) 97
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 2 cut(s) 14, 26
NciI CCSGG 2 cut(s) 660, 670
NdeII GATC 4 cut(s) 162, 472, 501, 665
NlaIV GGNNCC 3 cut(s) 231, 232, 667
NspV TTCGAA 1 cut(s) 538
PaqCI CACCTGC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 331
PfoI TCCNGGA 2 cut(s) 658, 668
PkrI GCNGC 4 cut(s) 7, 19, 417, 439
PmeI GTTTAAAC 1 cut(s) 97
PpuMI RGGWCCY 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 230
PspN4I GGNNCC 3 cut(s) 231, 232, 667
PspPI GGNCC 2 cut(s) 230, 590
PspPPI RGGWCCY 1 cut(s) 230
PstI CTGCAG 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 334
PsuI RGATCY 2 cut(s) 162, 665
RsaI GTAC 4 cut(s) 39, 345, 496, 531
RsaNI GTAC 4 cut(s) 38, 344, 495, 530
SaqAI TTAA 5 cut(s) 45, 66, 96, 479, 621
SatI GCNGC 4 cut(s) 6, 18, 416, 438
Sau3AI GATC 4 cut(s) 162, 472, 501, 665
Sau96I GGNCC 2 cut(s) 230, 590
ScrFI CCNGG 2 cut(s) 660, 670
SduI GDGCHC 1 cut(s) 726
SfaNI GCATC 1 cut(s) 142
SfcI CTRYAG 1 cut(s) 18
SfuI TTCGAA 1 cut(s) 538
SinI GGWCC 1 cut(s) 230
SmlI CTYRAG 2 cut(s) 245, 620
SmoI CTYRAG 2 cut(s) 245, 620
Sse9I AATT 4 cut(s) 311, 476, 533, 602
SsiI CCGC 5 cut(s) 5, 418, 437, 498, 563
SspMI CTAG 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 658, 668
StyI CCWWGG 1 cut(s) 520
TaaI ACNGT 4 cut(s) 296, 348, 409, 577
TaqI TCGA 3 cut(s) 122, 150, 538
TasI AATT 4 cut(s) 311, 476, 533, 602
TatI WGTACW 1 cut(s) 343
TauI GCSGC 2 cut(s) 8, 440
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 5 cut(s) 45, 66, 96, 479, 621
Tru9I TTAA 5 cut(s) 45, 66, 96, 479, 621
TscAI CASTG 2 cut(s) 357, 736
TseI GCWGC 2 cut(s) 17, 415
TspDTI ATGAA 3 cut(s) 72, 476, 626
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 2 cut(s) 357, 736
Vha464I CTTAAG 1 cut(s) 620
VpaK11BI GGWCC 1 cut(s) 230
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.