Rorug04G0289000

Protease Do-like 8

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
Physical Location & Seq
Reverse (-)
46220662 .. 46221201
540 bp
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UTR
Exon/CDS
Intron
Rorug04G0289000.1

Sequence Viewer

Length: 294 bp
ATGATCAGGACTATACCTGTGATCGTCGGCTGCTTTCTGTTAATATTGGCCTTTCTGGACGCTGATGTTTTGCAAGATAAACGTCACTTGGCTCAAGTCAAGAACGATCCGATACAGGGAAAAATCATTGAGGATCTTGCTAAGCATGATGATGATGCTTCGATCACAAACAGCCATGATGAAGATGATGATGCTCCGATCATGACAAACAACCATGATGATGATGAAGATGGTCCTTACTATACGGACCGTAGATTTAGACCCCATTATATCAACCCCTCTAAGCATGATTAG

Protein Analysis

97

Amino Acids

11.14

Weight (kDa)

4.64

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 101, 141
AfiI CCNNNNNNNGG 1 cut(s) 116
AlwI GGATC 2 cut(s) 101, 141
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 233, 247
AvaII GGWCC 2 cut(s) 233, 247
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 224
BclI TGATCA 1 cut(s) 3
BisI GCNGC 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 1 cut(s) 32
Bme18I GGWCC 2 cut(s) 233, 247
BmgT120I GGNCC 2 cut(s) 233, 247
BmsI GCATC 2 cut(s) 145, 181
Bpu1102I GCTNAGC 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 78
Bsc4I CCNNNNNNNGG 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 116
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 116
BsnI GGCC 1 cut(s) 50
Bsp143I GATC 6 cut(s) 3, 21, 106, 133, 162, 198
Bsp1720I GCTNAGC 1 cut(s) 141
BspANI GGCC 1 cut(s) 50
BspHI TCATGA 1 cut(s) 201
BspPI GGATC 2 cut(s) 101, 141
BssMI GATC 6 cut(s) 3, 21, 106, 133, 162, 198
Bst4CI ACNGT 1 cut(s) 251
BstDEI CTNAG 2 cut(s) 141, 282
BstKTI GATC 6 cut(s) 6, 24, 109, 136, 165, 201
BstMBI GATC 6 cut(s) 3, 21, 106, 133, 162, 198
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 1 cut(s) 50
CciI TCATGA 1 cut(s) 201
Cfr13I GGNCC 2 cut(s) 233, 247
CpoI CGGWCCG 1 cut(s) 247
CseI GACGC 1 cut(s) 68
CspI CGGWCCG 1 cut(s) 247
CviAII CATG 5 cut(s) 146, 176, 202, 215, 287
CviJI RGCY 4 cut(s) 30, 50, 92, 174
CviKI_1 RGCY 4 cut(s) 30, 50, 92, 174
DdeI CTNAG 2 cut(s) 141, 282
DpnI GATC 6 cut(s) 5, 23, 108, 135, 164, 200
DpnII GATC 6 cut(s) 3, 21, 106, 133, 162, 198
Eco47I GGWCC 2 cut(s) 233, 247
FaeI CATG 5 cut(s) 149, 179, 205, 218, 290
FaiI YATR 8 cut(s) 14, 147, 177, 203, 216, 243, 270, 288
FatI CATG 5 cut(s) 145, 175, 201, 214, 286
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 1 cut(s) 31
Fsp4HI GCNGC 1 cut(s) 31
GluI GCNGC 1 cut(s) 31
HaeIII GGCC 1 cut(s) 50
HgaI GACGC 1 cut(s) 68
Hin1II CATG 5 cut(s) 149, 179, 205, 218, 290
Hpy188I TCNGA 2 cut(s) 111, 198
Hpy188III TCNNGA 4 cut(s) 7, 56, 100, 202
Hpy99I CGWCG 1 cut(s) 29
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 82
HpyCH4V TGCA 1 cut(s) 73
HpyF3I CTNAG 2 cut(s) 141, 282
HpySE526I ACGT 1 cut(s) 82
Hsp92II CATG 5 cut(s) 149, 179, 205, 218, 290
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 6 cut(s) 3, 21, 106, 133, 162, 198
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 3 cut(s) 30, 41, 101
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 2 cut(s) 145, 181
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 1 cut(s) 83
MalI GATC 6 cut(s) 5, 23, 108, 135, 164, 200
MboI GATC 6 cut(s) 3, 21, 106, 133, 162, 198
MboII GAAGA 2 cut(s) 194, 239
MflI RGATCY 1 cut(s) 133
MnlI CCTC 2 cut(s) 124, 289
MseI TTAA 1 cut(s) 41
MslI CAYNNNNRTG 2 cut(s) 150, 219
NdeII GATC 6 cut(s) 3, 21, 106, 133, 162, 198
NlaIII CATG 5 cut(s) 149, 179, 205, 218, 290
NmuCI GTSAC 1 cut(s) 83
PagI TCATGA 1 cut(s) 201
PkrI GCNGC 1 cut(s) 32
PspPI GGNCC 2 cut(s) 233, 247
PsuI RGATCY 1 cut(s) 133
RseI CAYNNNNRTG 2 cut(s) 150, 219
Rsr2I CGGWCCG 1 cut(s) 247
RsrII CGGWCCG 1 cut(s) 247
SaqAI TTAA 1 cut(s) 41
SatI GCNGC 1 cut(s) 31
Sau3AI GATC 6 cut(s) 3, 21, 106, 133, 162, 198
Sau96I GGNCC 2 cut(s) 233, 247
SetI ASST 2 cut(s) 19, 85
SfaNI GCATC 2 cut(s) 145, 181
SinI GGWCC 2 cut(s) 233, 247
SmiMI CAYNNNNRTG 2 cut(s) 150, 219
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
SspI AATATT 1 cut(s) 45
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 1 cut(s) 85
TaqI TCGA 1 cut(s) 161
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TseFI GTSAC 1 cut(s) 83
TseI GCWGC 1 cut(s) 30
Tsp45I GTSAC 1 cut(s) 83
TspDTI ATGAA 2 cut(s) 195, 240
TspGWI ACGGA 1 cut(s) 260
VpaK11BI GGWCC 2 cut(s) 233, 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.