Rh2AG169600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013773
Physical Location & Seq
Forward (+)
16069540 .. 16074376
4837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG169600.1

Sequence Viewer

Length: 1110 bp
ATGAGAGAATACAGCCCACTTCACTCCCACAATAAACAATACCGAATCATCCAAACTCTATTATGTGTGCTCTTCGAGGATTCTTTAGACAGTGAGAGCGGAAGCTGCGCCACGGTAAATGGAAACCGGAAAAGAAAGAAGCAAGCAGGGTGGCTCTATCCATTTCAGGTGTCATCAAATGAAGAAGAAGGGTCTTCTCGAATGTTAGTGGCCTCCAACTTGGAGCTTGCTGGGTCAGTCAATGCTAAAGTCAATGGATTCCCAATTGGAAATTCGGAGTTTCACATTGAATATGTCCCCCGTTACCAAGCTTTTTATTCGGGTCTTTGCATCGCTGGACTTCACCAATTACCAAGATCAAGAATGGCGGACCTCGCTGCAGGTGGTGCTATGGGAGTGCCGTTTACTATGCTCTATGAAGTGATTAAGGACGTGGTGATCAAGACTTCGATGTTTGAACCTCTCCTTGAAGAGCTGAAGTCGAAGATAGAAGCTTTGAATCCACTGATTGATAAGATGGACAGCTGGAATAAGGTGTTGGATCGTCCAGAGAAGGAACTTAGACAATTTAAAGTACAAATGGAGAAGGGCCGTGAGCTTATTACCAAGTGCGCCAAGCTTAGCAAGCTTAGCAAGTGGAACAGCTACAAAAAGTATAAATACAGCTACCAACTTCTTGAATTGCAGAACTCTCTTCAGAATCTGCTGTTTATACTGCAATGGCAACAAGCAAGGGATGTGAAGGAGACCTTGGTTGTAGCAAAGAATGTAGAGACGGTGGTGCAGCGAGTGGATGTCAGTGTTGCGGAATTGAAAGAAGAAGTTATGAAGGTGACCTTGGATAGAAATATAGGGACGATGGTCCAGCGGATTGATAAGAAAGGTGAGGTACAGAGCCAAATAGATTGTGAAGTACCTGAACCTCCGTTGGTTACAGTTGGATTGGATCTGCCTTTGAGGGAATTGAAGATGAAGCTTCTTAAGAATGATAATGTGTCAATGCTTGTGCTAACTGCTCCTGGAGGCTGCGGGAAAACCACATTGGCGACAAAGTTTTGCCAAGATGAGGAAGTCAAAGAATGCAAGAATCCAACTGAGGTTGAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

369

Amino Acids

41.89

Weight (kDa)

7.99

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 123 - 261 1.8e-28 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 371
Acc36I ACCTGC 1 cut(s) 371
AccBSI CCGCTC 1 cut(s) 99
AciI CCGC 5 cut(s) 99, 368, 806, 868, 1029
AclWI GGATC 2 cut(s) 549, 954
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 2 cut(s) 497, 680
AfaI GTAC 3 cut(s) 576, 891, 915
AfiI CCNNNNNNNGG 1 cut(s) 1066
AflII CTTAAG 1 cut(s) 980
AgsI TTSAA 7 cut(s) 290, 458, 470, 499, 680, 814, 967
AjiI CACGTC 1 cut(s) 433
AjnI CCWGG 1 cut(s) 1018
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 2 cut(s) 740, 767
AlwI GGATC 2 cut(s) 549, 954
AlwNI CAGNNNCTG 1 cut(s) 703
AoxI GGCC 2 cut(s) 210, 589
ApeKI GCWGC 4 cut(s) 105, 377, 784, 1026
ApoI RAATTY 1 cut(s) 271
AspLEI GCGC 2 cut(s) 110, 614
AspS9I GGNCC 3 cut(s) 370, 589, 862
AsuHPI GGTGA 4 cut(s) 335, 448, 844, 896
AvaII GGWCC 2 cut(s) 370, 862
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 4 cut(s) 92, 364, 796, 1013
BccI CCATC 2 cut(s) 511, 853
BceAI ACGGC 2 cut(s) 385, 576
BciT130I CCWGG 1 cut(s) 1020
BclI TGATCA 1 cut(s) 438
BcoDI GTCTC 2 cut(s) 740, 767
BfmI CTRYAG 1 cut(s) 378
BfrI CTTAAG 1 cut(s) 980
BfuAI ACCTGC 1 cut(s) 371
BisI GCNGC 4 cut(s) 106, 378, 785, 1027
BlpI GCTNAGC 2 cut(s) 620, 629
BlsI GCNGC 4 cut(s) 107, 379, 786, 1028
Bme1390I CCNGG 1 cut(s) 1020
Bme18I GGWCC 2 cut(s) 370, 862
BmgBI CACGTC 1 cut(s) 433
BmgT120I GGNCC 3 cut(s) 370, 589, 862
BmrFI CCNGG 1 cut(s) 1020
BmsI GCATC 1 cut(s) 339
BoxI GACNNNNGTC 1 cut(s) 860
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 1041
Bpu1102I GCTNAGC 2 cut(s) 620, 629
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 3 cut(s) 111, 750, 837
BsaWI WCCGGW 1 cut(s) 126
Bsc4I CCNNNNNNNGG 1 cut(s) 1066
Bse3DI GCAATG 1 cut(s) 725
BseBI CCWGG 1 cut(s) 1020
BseDI CCNNGG 3 cut(s) 111, 750, 837
BseGI GGATG 3 cut(s) 48, 742, 799
BseLI CCNNNNNNNGG 1 cut(s) 1066
BseMI GCAATG 1 cut(s) 725
BseMII CTCAG 1 cut(s) 1086
BseXI GCAGC 4 cut(s) 92, 364, 796, 1013
BseYI CCCAGC 1 cut(s) 230
BsgI GTGCAG 1 cut(s) 803
BshFI GGCC 2 cut(s) 212, 591
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 127
BslFI GGGAC 2 cut(s) 281, 868
BslI CCNNNNNNNGG 1 cut(s) 1066
BsmAI GTCTC 2 cut(s) 740, 767
BsmBI CGTCTC 1 cut(s) 767
BsmFI GGGAC 2 cut(s) 281, 868
BsmI GAATGC 1 cut(s) 1085
BsnI GGCC 2 cut(s) 212, 591
Bso31I GGTCTC 1 cut(s) 740
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 4 cut(s) 356, 438, 541, 946
Bsp1720I GCTNAGC 2 cut(s) 620, 629
BspACI CCGC 5 cut(s) 99, 368, 806, 868, 1029
BspANI GGCC 2 cut(s) 212, 591
BspCNI CTCAG 1 cut(s) 1087
BspMAI CTGCAG 1 cut(s) 382
BspMI ACCTGC 1 cut(s) 371
BspPI GGATC 2 cut(s) 549, 954
BspQI GCTCTTC 2 cut(s) 77, 465
BspTI CTTAAG 1 cut(s) 980
BspTNI GGTCTC 1 cut(s) 740
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 725
BssECI CCNNGG 3 cut(s) 111, 750, 837
BssMI GATC 4 cut(s) 356, 438, 541, 946
BssT1I CCWWGG 2 cut(s) 750, 837
Bst2UI CCWGG 1 cut(s) 1020
Bst4CI ACNGT 4 cut(s) 92, 115, 778, 937
Bst6I CTCTTC 3 cut(s) 77, 465, 699
BstAFI CTTAAG 1 cut(s) 980
BstAPI GCANNNNNTGC 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 144, 228, 626
BstDEI CTNAG 4 cut(s) 560, 620, 629, 1095
BstDSI CCRYGG 1 cut(s) 111
BstEII GGTNACC 1 cut(s) 832
BstF5I GGATG 3 cut(s) 48, 742, 799
BstHHI GCGC 2 cut(s) 110, 614
BstKTI GATC 4 cut(s) 359, 441, 544, 949
BstMAI GTCTC 2 cut(s) 740, 767
BstMBI GATC 4 cut(s) 356, 438, 541, 946
BstMWI GCNNNNNNNGC 5 cut(s) 105, 374, 386, 625, 630
BstNI CCWGG 1 cut(s) 1020
BstPAI GACNNNNGTC 1 cut(s) 860
BstPI GGTNACC 1 cut(s) 832
BstSCI CCNGG 1 cut(s) 1018
BstSFI CTRYAG 1 cut(s) 378
BstV1I GCAGC 4 cut(s) 92, 364, 796, 1013
BstV2I GAAGAC 1 cut(s) 186
BstX2I RGATCY 1 cut(s) 946
BstYI RGATCY 1 cut(s) 946
BsuRI GGCC 2 cut(s) 212, 591
BtgI CCRYGG 1 cut(s) 111
BtgZI GCGATG 1 cut(s) 316
BtrI CACGTC 1 cut(s) 433
BtsCI GGATG 3 cut(s) 48, 742, 799
BtsIMutI CAGTG 3 cut(s) 97, 503, 805
BveI ACCTGC 1 cut(s) 371
Cac8I GCNNGC 3 cut(s) 144, 228, 626
CaiI CAGNNNCTG 1 cut(s) 703
CfoI GCGC 2 cut(s) 110, 614
Cfr13I GGNCC 3 cut(s) 370, 589, 862
Csp6I GTAC 3 cut(s) 575, 890, 914
CspCI CAANNNNNGTGG 2 cut(s) 131, 166
CviQI GTAC 3 cut(s) 575, 890, 914
DdeI CTNAG 4 cut(s) 560, 620, 629, 1095
DpnI GATC 4 cut(s) 358, 440, 543, 948
DpnII GATC 4 cut(s) 356, 438, 541, 946
DraI TTTAAA 1 cut(s) 571
Eam1104I CTCTTC 3 cut(s) 77, 465, 699
EarI CTCTTC 3 cut(s) 77, 465, 699
EciI GGCGGA 1 cut(s) 383
Eco130I CCWWGG 2 cut(s) 750, 837
Eco31I GGTCTC 1 cut(s) 740
Eco47I GGWCC 2 cut(s) 370, 862
Eco57I CTGAAG 2 cut(s) 497, 680
Eco91I GGTNACC 1 cut(s) 832
EcoO65I GGTNACC 1 cut(s) 832
EcoRII CCWGG 1 cut(s) 1018
EcoT14I CCWWGG 2 cut(s) 750, 837
ErhI CCWWGG 2 cut(s) 750, 837
Esp3I CGTCTC 1 cut(s) 767
FaiI YATR 9 cut(s) 64, 294, 392, 410, 417, 657, 713, 827, 851
FalI AAGNNNNNCTT 4 cut(s) 734, 766, 821, 853
FaqI GGGAC 2 cut(s) 281, 868
FauI CCCGC 1 cut(s) 1022
FbaI TGATCA 1 cut(s) 438
Fnu4HI GCNGC 4 cut(s) 106, 378, 785, 1027
FokI GGATG 3 cut(s) 35, 749, 806
Fsp4HI GCNGC 4 cut(s) 106, 378, 785, 1027
GlaI GCGC 2 cut(s) 109, 613
GluI GCNGC 4 cut(s) 106, 378, 785, 1027
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 1041
HaeIII GGCC 2 cut(s) 212, 591
HapII CCGG 1 cut(s) 127
HhaI GCGC 2 cut(s) 110, 614
Hin6I GCGC 2 cut(s) 108, 612
HinP1I GCGC 2 cut(s) 108, 612
HindIII AAGCTT 5 cut(s) 309, 492, 617, 626, 974
HinfI GANTC 6 cut(s) 45, 80, 258, 499, 700, 1087
HpaII CCGG 1 cut(s) 127
HphI GGTGA 4 cut(s) 335, 448, 844, 896
Hpy166II GTNNAC 1 cut(s) 405
Hpy188I TCNGA 3 cut(s) 277, 699, 1109
Hpy188III TCNNGA 5 cut(s) 198, 360, 442, 548, 677
Hpy8I GTNNAC 1 cut(s) 405
HpyAV CCTTC 5 cut(s) 182, 547, 580, 736, 823
HpyCH4III ACNGT 4 cut(s) 92, 115, 778, 937
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 6 cut(s) 330, 380, 685, 718, 784, 1083
HpyF10VI GCNNNNNNNGC 5 cut(s) 105, 374, 386, 625, 630
HpyF3I CTNAG 4 cut(s) 560, 620, 629, 1095
HpySE526I ACGT 1 cut(s) 432
HspAI GCGC 2 cut(s) 108, 612
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 4 cut(s) 356, 438, 541, 946
LguI GCTCTTC 2 cut(s) 77, 465
LmnI GCTCC 2 cut(s) 223, 1021
Lsp1109I GCAGC 4 cut(s) 92, 364, 796, 1013
LweI GCATC 1 cut(s) 339
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 3 cut(s) 302, 832, 931
MalI GATC 4 cut(s) 358, 440, 543, 948
MbiI CCGCTC 1 cut(s) 99
MboI GATC 4 cut(s) 356, 438, 541, 946
MboII GAAGA 9 cut(s) 64, 186, 194, 197, 482, 496, 686, 830, 979
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 946
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 7 cut(s) 264, 271, 347, 566, 680, 809, 962
MmeI TCCRAC 3 cut(s) 240, 519, 919
MseI TTAA 3 cut(s) 426, 570, 981
MspA1I CMGCKG 2 cut(s) 525, 868
MspCI CTTAAG 1 cut(s) 980
MspI CCGG 1 cut(s) 127
MspR9I CCNGG 1 cut(s) 1020
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 1085
MvaI CCWGG 1 cut(s) 1020
MwoI GCNNNNNNNGC 5 cut(s) 105, 374, 386, 625, 630
NdeII GATC 4 cut(s) 356, 438, 541, 946
NmuCI GTSAC 1 cut(s) 832
PaqCI CACCTGC 1 cut(s) 371
PciSI GCTCTTC 2 cut(s) 77, 465
PctI GAATGC 1 cut(s) 1085
PfeI GAWTC 6 cut(s) 45, 80, 258, 499, 700, 1087
PfoI TCCNGGA 1 cut(s) 1018
PkrI GCNGC 4 cut(s) 107, 379, 786, 1028
PshAI GACNNNNGTC 1 cut(s) 860
Psp6I CCWGG 1 cut(s) 1018
PspEI GGTNACC 1 cut(s) 832
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 1018
PspPI GGNCC 3 cut(s) 370, 589, 862
PstI CTGCAG 1 cut(s) 382
PstNI CAGNNNCTG 1 cut(s) 703
PsuI RGATCY 1 cut(s) 946
PvuII CAGCTG 1 cut(s) 525
RsaI GTAC 3 cut(s) 576, 891, 915
RsaNI GTAC 3 cut(s) 575, 890, 914
SapI GCTCTTC 2 cut(s) 77, 465
SaqAI TTAA 3 cut(s) 426, 570, 981
SatI GCNGC 4 cut(s) 106, 378, 785, 1027
Sau3AI GATC 4 cut(s) 356, 438, 541, 946
Sau96I GGNCC 3 cut(s) 370, 589, 862
ScrFI CCNGG 1 cut(s) 1020
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 1 cut(s) 339
SfcI CTRYAG 1 cut(s) 378
SinI GGWCC 2 cut(s) 370, 862
SmlI CTYRAG 1 cut(s) 980
SmoI CTYRAG 1 cut(s) 980
Sse9I AATT 7 cut(s) 264, 271, 347, 566, 680, 809, 962
SsiI CCGC 5 cut(s) 99, 368, 806, 868, 1029
StyD4I CCNGG 1 cut(s) 1018
StyI CCWWGG 2 cut(s) 750, 837
TaaI ACNGT 4 cut(s) 92, 115, 778, 937
TaiI ACGT 1 cut(s) 435
TaqI TCGA 4 cut(s) 75, 199, 449, 482
TasI AATT 7 cut(s) 264, 271, 347, 566, 680, 809, 962
TatI WGTACW 1 cut(s) 574
TfiI GAWTC 6 cut(s) 45, 80, 258, 499, 700, 1087
Tru1I TTAA 3 cut(s) 426, 570, 981
Tru9I TTAA 3 cut(s) 426, 570, 981
TscAI CASTG 3 cut(s) 97, 510, 805
TseFI GTSAC 1 cut(s) 832
TseI GCWGC 4 cut(s) 105, 377, 784, 1026
Tsp45I GTSAC 1 cut(s) 832
TspDTI ATGAA 4 cut(s) 195, 432, 842, 986
TspGWI ACGGA 1 cut(s) 915
TspRI CASTG 3 cut(s) 97, 510, 805
Vha464I CTTAAG 1 cut(s) 980
VpaK11BI GGWCC 2 cut(s) 370, 862
XapI RAATTY 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.