RLG00000018228

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002677
Physical Location & Seq
Forward (+)
27202640 .. 27207115
4476 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018228

Sequence Viewer

Length: 849 bp
ATGCTTCTTGAAATGGTTTTTTTCCAGCAAAATATGACCCAAAAAGCTCCTCTCTCTTTCTTCTCCACTCTTCGTCTCTCTCAGTCAGTCCGCTCCAAATTCTCTCTCTTTGTTCACCTAAACCACAATTCACCCTTTCCATCATTACCCATTAGCAAATTGACAGTTCACATGGGAAGAAAAAGAGGTAAGAAAACTGTACAGAAAGTCGCTGAGGACATTGAGCCCCAGAATGAGAGGCAGGAAGATCAGTTTACGGACCCAGATGTTGAGCGAAGAATTTCTGCCGTTAAGGCTATTAGTGACGTGGAGATTGAACACGTGCTGACCAAATTGCGTTTGCTTCGCTCGTATTGCACCAAGGAGCAGCGGCAAACACCTGTTTTGCAATTTTTTAAAGAAAATTTTCCGAATTTGAAATTTGTAAAAAATGAAGAAAATGGACATCTAGAACTTGAGTGGATTGGTGACGATGGCAAAGTATCCATGAATGATGGAACTGATTTACATTTATCTCTTCTTAACCAGTTCTCTATGGCTCATTCTGGTTGCCAAACTTTGGGTGGCTTTGAGTTTTCTAGTAAAGCAGAGAGATCAAGGTTTTTGGGTGCTGATAATCTGCAGATCAAAGATTTTGTTTTAGAGGAGCCATTTGATACTCAGATGCTGGGGCAGCATGATTCTCTCCAAACTCCCGGGGTTAGTAGCCAGAGGCTATCTGTTGGGGTCACACCCAAAACGCGAAGGCTCCCAAAGCCTGGGGAGACCCTTTTATCTGTCCATGGATCGCCCCTCGGTGTTTACAAGGAAGATGATATGGAAGCCATAAATGAGTCAGAAGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

32.04

Weight (kDa)

6.02

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 559, 758
AccBSI CCGCTC 1 cut(s) 93
AccII CGCG 1 cut(s) 742
AciI CCGC 2 cut(s) 91, 370
AclWI GGATC 1 cut(s) 793
AcsI RAATTY 5 cut(s) 98, 279, 403, 412, 419
AcvI CACGTG 1 cut(s) 322
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 2 cut(s) 559, 758
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 3 cut(s) 11, 317, 418
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 1 cut(s) 757
AluBI AGCT 1 cut(s) 47
AluI AGCT 1 cut(s) 47
Alw26I GTCTC 2 cut(s) 80, 758
AlwI GGATC 1 cut(s) 793
AlwNI CAGNNNCTG 1 cut(s) 667
Ama87I CYCGRG 1 cut(s) 695
ApeKI GCWGC 2 cut(s) 367, 673
ApoI RAATTY 5 cut(s) 98, 279, 403, 412, 419
Asp700I GAANNNNTTC 2 cut(s) 280, 405
AspS9I GGNCC 1 cut(s) 259
AsuC2I CCSGG 2 cut(s) 696, 697
AsuHPI GGTGA 3 cut(s) 107, 123, 479
AvaI CYCGRG 1 cut(s) 695
AvaII GGWCC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 228
BbrPI CACGTG 1 cut(s) 322
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 379, 685
BccI CCATC 3 cut(s) 148, 467, 488
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 1 cut(s) 759
BciVI GTATCC 1 cut(s) 493
BcnI CCSGG 2 cut(s) 696, 697
BcoDI GTCTC 2 cut(s) 80, 758
BfaI CTAG 2 cut(s) 449, 579
BfmI CTRYAG 1 cut(s) 620
BfuI GTATCC 1 cut(s) 493
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 3 cut(s) 368, 371, 674
BlsI GCNGC 3 cut(s) 369, 372, 675
Bme1390I CCNGG 3 cut(s) 696, 697, 759
Bme18I GGWCC 1 cut(s) 259
BmeT110I CYCGRG 1 cut(s) 695
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 3 cut(s) 261, 648, 749
BmrFI CCNGG 3 cut(s) 696, 697, 759
BmsI GCATC 1 cut(s) 654
Bpu10I CCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 476
BpuMI CCSGG 2 cut(s) 696, 697
BsaAI YACGTR 1 cut(s) 322
BsaI GGTCTC 1 cut(s) 758
BsaJI CCNNGG 6 cut(s) 360, 695, 696, 758, 781, 793
Bsc4I CCNNNNNNNGG 2 cut(s) 559, 758
Bse1I ACTGG 1 cut(s) 526
BseBI CCWGG 1 cut(s) 759
BseDI CCNNGG 6 cut(s) 360, 695, 696, 758, 781, 793
BseLI CCNNNNNNNGG 2 cut(s) 559, 758
BseMII CTCAG 3 cut(s) 95, 204, 674
BseNI ACTGG 1 cut(s) 526
BseRI GAGGAG 2 cut(s) 39, 659
BseXI GCAGC 2 cut(s) 379, 685
BseYI CCCAGC 1 cut(s) 667
Bsh1236I CGCG 1 cut(s) 742
BsiHKCI CYCGRG 1 cut(s) 695
BsiSI CCGG 1 cut(s) 696
BslI CCNNNNNNNGG 2 cut(s) 559, 758
BsmAI GTCTC 2 cut(s) 80, 758
BsmBI CGTCTC 1 cut(s) 80
Bso31I GGTCTC 1 cut(s) 758
BsoBI CYCGRG 1 cut(s) 695
Bsp1286I GDGCHC 1 cut(s) 228
Bsp1407I TGTACA 1 cut(s) 199
Bsp143I GATC 4 cut(s) 247, 593, 624, 785
Bsp19I CCATGG 1 cut(s) 781
BspACI CCGC 2 cut(s) 91, 370
BspCNI CTCAG 3 cut(s) 94, 205, 673
BspFNI CGCG 1 cut(s) 742
BspLI GGNNCC 3 cut(s) 261, 648, 749
BspMAI CTGCAG 1 cut(s) 624
BspPI GGATC 1 cut(s) 793
BspTNI GGTCTC 1 cut(s) 758
BsrBI CCGCTC 1 cut(s) 93
BsrGI TGTACA 1 cut(s) 199
BsrI ACTGG 1 cut(s) 526
BssECI CCNNGG 6 cut(s) 360, 695, 696, 758, 781, 793
BssMI GATC 4 cut(s) 247, 593, 624, 785
BssT1I CCWWGG 2 cut(s) 360, 781
Bst2UI CCWGG 1 cut(s) 759
Bst4CI ACNGT 2 cut(s) 166, 199
Bst6I CTCTTC 2 cut(s) 75, 522
BstAUI TGTACA 1 cut(s) 199
BstBAI YACGTR 1 cut(s) 322
BstDEI CTNAG 3 cut(s) 81, 213, 660
BstDSI CCRYGG 1 cut(s) 781
BstFNI CGCG 1 cut(s) 742
BstKTI GATC 4 cut(s) 250, 596, 627, 788
BstMAI GTCTC 2 cut(s) 80, 758
BstMBI GATC 4 cut(s) 247, 593, 624, 785
BstMWI GCNNNNNNNGC 4 cut(s) 293, 354, 673, 754
BstNI CCWGG 1 cut(s) 759
BstSCI CCNGG 3 cut(s) 694, 695, 757
BstSFI CTRYAG 1 cut(s) 620
BstUI CGCG 1 cut(s) 742
BstV1I GCAGC 2 cut(s) 379, 685
BsuI GTATCC 1 cut(s) 493
BtgI CCRYGG 1 cut(s) 781
BtrI CACGTC 1 cut(s) 307
CaiI CAGNNNCTG 1 cut(s) 667
Cfr13I GGNCC 1 cut(s) 259
Cfr9I CCCGGG 1 cut(s) 695
Csp6I GTAC 1 cut(s) 200
CviAII CATG 4 cut(s) 172, 487, 677, 782
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 3 cut(s) 81, 213, 660
DpnI GATC 4 cut(s) 249, 595, 626, 787
DpnII GATC 4 cut(s) 247, 593, 624, 785
DraI TTTAAA 1 cut(s) 397
Eam1104I CTCTTC 2 cut(s) 75, 522
EarI CTCTTC 2 cut(s) 75, 522
Eco130I CCWWGG 2 cut(s) 360, 781
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 758
Eco47I GGWCC 1 cut(s) 259
Eco72I CACGTG 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 695
EcoRII CCWGG 1 cut(s) 757
EcoT14I CCWWGG 2 cut(s) 360, 781
EcoT38I GRGCYC 1 cut(s) 228
ErhI CCWWGG 2 cut(s) 360, 781
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 4 cut(s) 175, 490, 680, 785
FaiI YATR 8 cut(s) 35, 173, 488, 536, 678, 783, 818, 827
FatI CATG 4 cut(s) 171, 486, 676, 781
Fnu4HI GCNGC 3 cut(s) 368, 371, 674
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 3 cut(s) 368, 371, 674
FspBI CTAG 2 cut(s) 449, 579
GluI GCNGC 3 cut(s) 368, 371, 674
GsaI CCCAGC 1 cut(s) 671
HapII CCGG 1 cut(s) 696
Hin1II CATG 4 cut(s) 175, 490, 680, 785
HinfI GANTC 2 cut(s) 680, 833
HpaII CCGG 1 cut(s) 696
HphI GGTGA 3 cut(s) 107, 123, 479
Hpy166II GTNNAC 4 cut(s) 115, 169, 255, 802
Hpy188I TCNGA 3 cut(s) 411, 663, 838
Hpy188III TCNNGA 2 cut(s) 8, 449
Hpy8I GTNNAC 4 cut(s) 115, 169, 255, 802
HpyAV CCTTC 2 cut(s) 738, 836
HpyCH4III ACNGT 2 cut(s) 166, 199
HpyCH4IV ACGT 2 cut(s) 306, 321
HpyCH4V TGCA 3 cut(s) 357, 388, 622
HpyF10VI GCNNNNNNNGC 4 cut(s) 293, 354, 673, 754
HpyF3I CTNAG 3 cut(s) 81, 213, 660
HpySE526I ACGT 2 cut(s) 306, 321
Hsp92II CATG 4 cut(s) 175, 490, 680, 785
Kzo9I GATC 4 cut(s) 247, 593, 624, 785
LmnI GCTCC 5 cut(s) 52, 98, 364, 646, 753
Lsp1109I GCAGC 2 cut(s) 379, 685
LweI GCATC 1 cut(s) 654
MaeI CTAG 2 cut(s) 449, 579
MaeII ACGT 2 cut(s) 306, 321
MaeIII GTNAC 3 cut(s) 302, 467, 727
MalI GATC 4 cut(s) 249, 595, 626, 787
MbiI CCGCTC 1 cut(s) 93
MboI GATC 4 cut(s) 247, 593, 624, 785
MboII GAAGA 8 cut(s) 52, 62, 189, 257, 288, 446, 509, 821
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 9 cut(s) 98, 127, 158, 279, 332, 389, 403, 412, 419
MlyI GAGTC 1 cut(s) 842
MnlI CCTC 7 cut(s) 60, 179, 208, 231, 637, 705, 803
MroXI GAANNNNTTC 2 cut(s) 280, 405
MseI TTAA 3 cut(s) 291, 396, 522
MspA1I CMGCKG 1 cut(s) 370
MspI CCGG 1 cut(s) 696
MspR9I CCNGG 3 cut(s) 696, 697, 759
MvaI CCWGG 1 cut(s) 759
MvnI CGCG 1 cut(s) 742
MwoI GCNNNNNNNGC 4 cut(s) 293, 354, 673, 754
NciI CCSGG 2 cut(s) 696, 697
NcoI CCATGG 1 cut(s) 781
NdeII GATC 4 cut(s) 247, 593, 624, 785
NlaIII CATG 4 cut(s) 175, 490, 680, 785
NlaIV GGNNCC 3 cut(s) 261, 648, 749
NmuCI GTSAC 3 cut(s) 302, 467, 727
PdmI GAANNNNTTC 2 cut(s) 280, 405
PfeI GAWTC 1 cut(s) 680
PflMI CCANNNNNTGG 2 cut(s) 559, 758
PkrI GCNGC 3 cut(s) 369, 372, 675
PleI GAGTC 1 cut(s) 841
PmaCI CACGTG 1 cut(s) 322
PmlI CACGTG 1 cut(s) 322
PpsI GAGTC 1 cut(s) 841
Ppu21I YACGTR 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 757
PspCI CACGTG 1 cut(s) 322
PspFI CCCAGC 1 cut(s) 667
PspGI CCWGG 1 cut(s) 757
PspN4I GGNNCC 3 cut(s) 261, 648, 749
PspPI GGNCC 1 cut(s) 259
PstI CTGCAG 1 cut(s) 624
PstNI CAGNNNCTG 1 cut(s) 667
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
SaqAI TTAA 3 cut(s) 291, 396, 522
SatI GCNGC 3 cut(s) 368, 371, 674
Sau3AI GATC 4 cut(s) 247, 593, 624, 785
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 1 cut(s) 842
ScrFI CCNGG 3 cut(s) 696, 697, 759
SduI GDGCHC 1 cut(s) 228
SetI ASST 7 cut(s) 49, 120, 190, 309, 324, 382, 602
SfaNI GCATC 1 cut(s) 654
SfcI CTRYAG 1 cut(s) 620
SinI GGWCC 1 cut(s) 259
SmaI CCCGGG 1 cut(s) 697
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
Sse9I AATT 9 cut(s) 98, 127, 158, 279, 332, 389, 403, 412, 419
SsiI CCGC 2 cut(s) 91, 370
SspMI CTAG 2 cut(s) 449, 579
StyD4I CCNGG 3 cut(s) 694, 695, 757
StyI CCWWGG 2 cut(s) 360, 781
TaaI ACNGT 2 cut(s) 166, 199
TaiI ACGT 2 cut(s) 309, 324
TasI AATT 9 cut(s) 98, 127, 158, 279, 332, 389, 403, 412, 419
TatI WGTACW 1 cut(s) 199
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 1 cut(s) 680
Tru1I TTAA 3 cut(s) 291, 396, 522
Tru9I TTAA 3 cut(s) 291, 396, 522
TseFI GTSAC 3 cut(s) 302, 467, 727
TseI GCWGC 2 cut(s) 367, 673
Tsp45I GTSAC 3 cut(s) 302, 467, 727
TspDTI ATGAA 2 cut(s) 447, 503
TspGWI ACGGA 1 cut(s) 272
TspMI CCCGGG 1 cut(s) 695
Van91I CCANNNNNTGG 2 cut(s) 559, 758
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 5 cut(s) 98, 279, 403, 412, 419
XbaI TCTAGA 1 cut(s) 448
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XmaI CCCGGG 1 cut(s) 695
XmnI GAANNNNTTC 2 cut(s) 280, 405
XspI CTAG 2 cut(s) 449, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.