Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0010927

Physical Location & Seq

Genomic Coordinates Forward (+)

52802190 .. 52804661

Gene Structure 2472 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000019500

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2472 bp

ATGTCAAAGACTCTTCTCTCTCGCATCAAACCCCTTCACAGCCGCAAACCCACTTGTCCATCTTCCTATTCACCACCCCACATCAAGAAGCTAGTCCATGACACCATCCGGATTCTCACAACAGATGATAAATGGGAGCAGTCCCTCGAAACCGAGTTCTCCGAAACCGAAACGCTTGTTTCTGAAGTTGCCCATTTCGTTTTGGACAGAATACCCAATGCCGAGTTGGGTTTGAAGTTCTTTGACTGGGCTTTCCGGCGACCCTATTGCTGCTCTCCAAATGGGTCGGCGTATTCTTCTCTCTTGAAGCTTTTGGCGAGGTTTGGAGTGGTTGCGGAGATTGAGTTGGTGCTGCAGAGCATGAAGTTGGAACAAGTAGAGCCGAGTAAGGATGCTTTGAGCTTTGTGATTCAAACATATGCGGATTCTGGGTTGGTTGATAAGGCTCTTGAATTGTACAGTTTTGGGGTCAAGGTGTGTGGTTTTGTTCCGAGTGTTTTCGCATGCAATTCCTTGCTTAACACGCTTGTGAAGATTGGGAGAGTTGATGTTGCACGCCAGGTGTATGATGAAATGTCTGAGAAAGGTGGTGGTGATGGTGAGCATGTGTGTATAGATAACTACAGTACTTGTATTATGGTGAGGGGGTTGTGTAAGGAAGGGAAGGTTGAAGAAGGAAGAAGGTTGATTGAGGAGAGGTGGGGAGAGAATTGTGTGCCGAATGTTGTGTTCTACAATACGCTTATTGATGGATATTGTAAGAGAGGTGAGGTTGAAAATGCTAATGGGATGTTTAAGGAGTTGAAATTGAAGGGGTTTCTGCCTACATTGCAAACCTATGGAGCTTTGATTAATGGCTATTGCAAAGTAGGGAACTTTGTAGCGATTGATAGGCTTTTGATGGAAATGAAGGAGAGAGGTTTGAGGGTCAATGTACAGGTACATAATACTATAGTTGAAGCTAGATGTAAGCACGATAGCTGTGCAACCGCGGTGGATACTGTAGCAAGGATGATTGAGAGTGGTTGTGAGCCGGATATTACAACCTATAATATTTTGATTAATAGTTCATGTAAGAAAAGGAAGGTTGAGGAAGCGGAGGAGTTTCTACAGCATGCAATGAAGAGGGGATTGGTTCCGAATGAGTTTAGTTATACTCCTGTTTTTCAAGTCTATTGCAGACGAGGGGAACACTGTAGGGCCCTGGATTTGCTTGTTGAGATGACAAAAAGAGGTCACAAACCTGATTTGGTTGCCTTTGGAGCTTTGATTCATGGACATGTTGTTTCTGGGGAAGTTGATTATGCATTGACAGTCCGGGACAGAATGATAGAAAATGGAATACTCCCCGATGCTTGCATTTACAATGTGTTGATGAGTGGCCTTTGCAAGAAAGGGAGGCTTCCTACTGCCAAACTGCTGCTTGGTCAGATGCTTGACCAAAATGTACTACCTGATGCATTTGTCTACGCCACATTGGTAGATGGGTTAATCAGAAATGGGAACCTTGAGGAGGCTAAAAATATATTCGAGTTAACAATTGAAAAGGGCCTAGATCCTGGTGTTGTGGGGTACAATGCTATGATCAAGGGTTTCTGCAAATTTGGAACAATGAATGATGCACTGTCATTCCTCGAAAAGATGAAGAAAAGGAATCATCATCCTGATGGGTTTACTTACTCCACAATAATTGATGGGTATGTCAAGCAGCATAATTTGGATGCTGCACTAGAAATGTTTAAACAGATGGTGAAACAGAGATGCAGGCCAAATGTCATTACGTACACCTCCTTGATCAATGGGTTTTGCCGCAAAGGAGATTCTGATGGAGCTGTAAAAACTTTTGTAGAGATGCAATCTCGTGGTGTGGAGCCAAATATAGTCACGTACAGCATACTTATTGGAAACTTTTGCAAGGAAGGTAAACTTGCAAAAGCAGCCTCCTTTTTTGAACTAATGCTGAGGAAAAAGTGCAGTCCTAATGATGTCACTATCCATTATTTGCTAAATGGGTTTACAAACATTGCACCTGCTGCAATTTCAAAGGAAGTGAATGAATCTCAAGAAAAGGAGAAATCTATTTTTCTTGATTTCTATAAAAGGATGATAGCCGATGGATGGTTTCAGAAGTCTGCTGTCTATAATTCCATTCTCATTTGTCTTTGTCAGTATGGAATGGTTGAAGCTGCATTACAGTTTAACAGTAAGTTTAGAAGTAAAGGTATTGATCTAGATTCTGTTTCTTTTGCGGCTGTGCTACATGGTCTTTTCTTGGGAGGAAGATCAAAGGACTGGAAGAATATCATTTATTTTAACTTGAAGGAAAAAGAACTCCAAACTGCTGTCAAATACTCGCGTATAATAGATGCACAGTTCCATGGAGGAAGAATATCTGAAGCTACACTTGCTTTACGGTCATTAGTTGAAGATGATAAGCCTCAACATCAAGAAGTGGAAGACAGAGAAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

His_Phos_1 PPR PPR_1 PPR_2 PPR_3 p450

Protein Analysis

824

Amino Acids

92.64

Weight (kDa)

7.86

Isoelectric Point (pI)

38.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	164 - 219	8.8e-08	PPR repeat family
PPR_3	PF13812	237 - 287	1.8e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	237 - 268	4.2e-11	PPR repeat
PPR_2	PF13041	240 - 289	9.5e-17	PPR repeat family
PPR	PF01535	243 - 273	1.4e-07	PPR repeat
PPR_2	PF13041	277 - 324	3.7e-08	PPR repeat family
PPR	PF01535	279 - 308	9.4e-06	PPR repeat
PPR_3	PF13812	298 - 355	2.1e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	311 - 359	2.1e-08	PPR repeat family
PPR_long	PF17177	328 - 466	7.3e-07	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	342 - 371	3.9e-10	PPR repeat
PPR_2	PF13041	345 - 394	1.4e-11	PPR repeat family
PPR_2	PF13041	384 - 427	3.7e-07	PPR repeat family
PPR_3	PF13812	404 - 460	5.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	446 - 478	3e-07	PPR repeat
PPR_2	PF13041	450 - 498	4.3e-10	PPR repeat family
PPR_3	PF13812	474 - 532	7.9e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	516 - 549	5.8e-09	PPR repeat
PPR_2	PF13041	520 - 569	6.2e-17	PPR repeat family
PPR	PF01535	525 - 552	2e-06	PPR repeat
PPR_3	PF13812	545 - 601	1e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	552 - 584	2.5e-07	PPR repeat
PPR	PF01535	558 - 588	2.8e-06	PPR repeat
PPR_2	PF13041	558 - 604	6.2e-17	PPR repeat family
PPR_long	PF17177	569 - 670	4.8e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	588 - 619	3.5e-12	PPR repeat
PPR_2	PF13041	590 - 639	2.6e-19	PPR repeat family
PPR_3	PF13812	591 - 638	1.1e-10	Pentatricopeptide repeat domain
PPR	PF01535	593 - 623	1.7e-08	PPR repeat
PPR_3	PF13812	616 - 672	4.7e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	621 - 653	6.6e-10	PPR repeat
PPR_2	PF13041	633 - 673	4.2e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010927)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52620
fragaria_vesca	FvH4_6g30030
malus_domestica	MD17G1208200.v1.1
prunus_persica	Prupe.3G082900_v2.0.a1
pyrus_communis	pycom17g21100
rosa_chinensis	RchiOBHm_Chr2g0136061 RchiOBHm_Chr2g0136071
rosa_laevigata	RLG00000019500
rosa_multiflora	Rmu_sc0002070.1_g000001 Rmu_sc0002070.1_g000002 Rmu_sc0002986.1_g000008 Rmu_sc0002986.1_g000009
rosa_roxburghii	Rroxscaffold_2G00108610
rosa_rugosa	Rorug02G0327800
rosa_samantha	Rh2AG379700 Rh2BG386200 Rh2CG365600 Rh2CG365700 Rh2DG402500
rosa_wichuraiana	Rw2G030950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2038
Acc36I	ACCTGC	1 cut(s)	2038
AccI	GTMKAC	1 cut(s)	1467
AccII	CGCG	2 cut(s)	992, 2356
AccIII	TCCGGA	1 cut(s)	108
AciI	CCGC	8 cut(s)	43, 335, 422, 990, 992, 1097, 1810, 2249
AclWI	GGATC	1 cut(s)	1550
AcsI	RAATTY	1 cut(s)	1601
AcuI	CTGAAG	2 cut(s)	204, 2415
AfaI	GTAC	8 cut(s)	458, 628, 936, 942, 1449, 1574, 1784, 1889
AfiI	CCNNNNNNNGG	2 cut(s)	1513, 2118
AflIII	ACRYGT	1 cut(s)	1279
AjnI	CCWGG	3 cut(s)	558, 1203, 1558
AjuI	GAANNNNNNNTTGG	2 cut(s)	1115, 1147
AleI	CACNNNNGTG	1 cut(s)	527
AlwI	GGATC	1 cut(s)	1550
Aor13HI	TCCGGA	1 cut(s)	108
AoxI	GGCC	4 cut(s)	1200, 1381, 1549, 1766
ApaI	GGGCCC	1 cut(s)	1204
ApeKI	GCWGC	8 cut(s)	270, 352, 1420, 1708, 1724, 1937, 2033, 2186
ApoI	RAATTY	1 cut(s)	1601
AseI	ATTAAT	2 cut(s)	852, 1062
AspS9I	GGNCC	3 cut(s)	1200, 1201, 1549
AsuC2I	CCSGG	1 cut(s)	1319
AsuHPI	GGTGA	6 cut(s)	63, 605, 611, 652, 779, 1762
BaeGI	GKGCMC	1 cut(s)	1204
BanII	GRGCYC	1 cut(s)	1204
BarI	GAAGNNNNNNTAC	2 cut(s)	2175, 2207
BauI	CACGAG	1 cut(s)	1860
BbsI	GAAGAC	1 cut(s)	2463
BbvCI	CCTCAGC	1 cut(s)	1961
BbvI	GCAGC	8 cut(s)	257, 339, 1407, 1711, 1720, 1949, 2020, 2173
BcgI	CGANNNNNNTGC	4 cut(s)	249, 283, 965, 999
BciT130I	CCWGG	3 cut(s)	560, 1205, 1560
BciVI	GTATCC	1 cut(s)	991
BclI	TGATCA	2 cut(s)	1584, 1794
BcnI	CCSGG	1 cut(s)	1319
BfaI	CTAG	5 cut(s)	92, 963, 1553, 1730, 2231
BfmI	CTRYAG	6 cut(s)	353, 622, 951, 1002, 1109, 1195
BfuAI	ACCTGC	1 cut(s)	2038
BfuI	GTATCC	1 cut(s)	991
BmcAI	AGTACT	1 cut(s)	628
Bme1390I	CCNGG	4 cut(s)	560, 1205, 1319, 1560
BmgT120I	GGNCC	3 cut(s)	1200, 1201, 1549
BmiI	GGNNCC	4 cut(s)	1137, 1202, 1505, 1872
BmrFI	CCNGG	4 cut(s)	560, 1205, 1319, 1560
BmrI	ACTGGG	1 cut(s)	256
BmuI	ACTGGG	1 cut(s)	256
BpiI	GAAGAC	1 cut(s)	2463
Bpu10I	CCTNAGC	1 cut(s)	1961
BpuEI	CTTGAG	2 cut(s)	1529, 2046
BpuMI	CCSGG	1 cut(s)	1319
BsaAI	YACGTR	2 cut(s)	1782, 1887
BsaJI	CCNNGG	3 cut(s)	990, 1203, 2377
BsaWI	WCCGGW	1 cut(s)	108
BsaXI	ACNNNNNCTCC	2 cut(s)	329, 359
Bsc4I	CCNNNNNNNGG	2 cut(s)	1513, 2118
Bse1I	ACTGG	2 cut(s)	251, 2297
Bse3DI	GCAATG	3 cut(s)	827, 1125, 2022
BseAI	TCCGGA	1 cut(s)	108
BseBI	CCWGG	3 cut(s)	560, 1205, 1560
BseDI	CCNNGG	3 cut(s)	990, 1203, 2377
BseGI	GGATG	8 cut(s)	105, 397, 795, 1017, 1660, 1726, 2109, 2123
BseLI	CCNNNNNNNGG	2 cut(s)	1513, 2118
BseMI	GCAATG	3 cut(s)	827, 1125, 2022
BseMII	CTCAG	2 cut(s)	570, 1952
BseNI	ACTGG	2 cut(s)	251, 2297
BseRI	GAGGAG	3 cut(s)	707, 1115, 1526
BseSI	GKGCMC	1 cut(s)	1204
BseXI	GCAGC	8 cut(s)	257, 339, 1407, 1711, 1720, 1949, 2020, 2173
BsgI	GTGCAG	2 cut(s)	1710, 1993
Bsh1236I	CGCG	2 cut(s)	992, 2356
BshFI	GGCC	4 cut(s)	1202, 1383, 1551, 1768
BsiSI	CCGG	4 cut(s)	109, 256, 1034, 1318
BslFI	GGGAC	2 cut(s)	127, 1334
BslI	CCNNNNNNNGG	2 cut(s)	1513, 2118
BsmFI	GGGAC	2 cut(s)	127, 1334
BsnI	GGCC	4 cut(s)	1202, 1383, 1551, 1768
Bsp120I	GGGCCC	1 cut(s)	1200
Bsp1286I	GDGCHC	1 cut(s)	1204
Bsp13I	TCCGGA	1 cut(s)	108
Bsp1407I	TGTACA	2 cut(s)	456, 934
Bsp143I	GATC	5 cut(s)	1555, 1584, 1794, 2227, 2282
Bsp19I	CCATGG	1 cut(s)	2377
BspACI	CCGC	8 cut(s)	43, 335, 422, 990, 992, 1097, 1810, 2249
BspANI	GGCC	4 cut(s)	1202, 1383, 1551, 1768
BspCNI	CTCAG	2 cut(s)	571, 1953
BspEI	TCCGGA	1 cut(s)	108
BspFNI	CGCG	2 cut(s)	992, 2356
BspLI	GGNNCC	4 cut(s)	1137, 1202, 1505, 1872
BspMAI	CTGCAG	1 cut(s)	357
BspMI	ACCTGC	1 cut(s)	2038
BspPI	GGATC	1 cut(s)	1550
BsrDI	GCAATG	3 cut(s)	827, 1125, 2022
BsrGI	TGTACA	2 cut(s)	456, 934
BsrI	ACTGG	2 cut(s)	251, 2297
BssECI	CCNNGG	3 cut(s)	990, 1203, 2377
BssMI	GATC	5 cut(s)	1555, 1584, 1794, 2227, 2282
BssSI	CACGAG	1 cut(s)	1860
BssT1I	CCWWGG	1 cut(s)	2377
Bst2BI	CACGAG	1 cut(s)	1860
Bst2UI	CCWGG	3 cut(s)	560, 1205, 1560
Bst6I	CTCTTC	2 cut(s)	18, 1118
BstAPI	GCANNNNNTGC	1 cut(s)	2033
BstAUI	TGTACA	2 cut(s)	456, 934
BstBAI	YACGTR	2 cut(s)	1782, 1887
BstC8I	GCNNGC	5 cut(s)	505, 556, 1116, 1357, 1766
BstDEI	CTNAG	2 cut(s)	579, 1961
BstDSI	CCRYGG	2 cut(s)	990, 2377
BstENI	CCTNNNNNAGG	1 cut(s)	1511
BstF5I	GGATG	8 cut(s)	105, 397, 795, 1017, 1660, 1726, 2109, 2123
BstFNI	CGCG	2 cut(s)	992, 2356
BstKTI	GATC	5 cut(s)	1558, 1587, 1797, 2230, 2285
BstMBI	GATC	5 cut(s)	1555, 1584, 1794, 2227, 2282
BstMWI	GCNNNNNNNGC	6 cut(s)	523, 829, 1262, 1937, 2033, 2405
BstNI	CCWGG	3 cut(s)	560, 1205, 1560
BstNSI	RCATGY	4 cut(s)	507, 608, 1118, 1283
BstSCI	CCNGG	4 cut(s)	558, 1203, 1317, 1558
BstSFI	CTRYAG	6 cut(s)	353, 622, 951, 1002, 1109, 1195
BstSLI	GKGCMC	1 cut(s)	1204
BstSNI	TACGTA	1 cut(s)	1782
BstUI	CGCG	2 cut(s)	992, 2356
BstV1I	GCAGC	8 cut(s)	257, 339, 1407, 1711, 1720, 1949, 2020, 2173
BstV2I	GAAGAC	1 cut(s)	2463
BstX2I	RGATCY	1 cut(s)	1555
BstYI	RGATCY	1 cut(s)	1555
BsuI	GTATCC	1 cut(s)	991
BsuRI	GGCC	4 cut(s)	1202, 1383, 1551, 1768
BtgI	CCRYGG	2 cut(s)	990, 2377
BtsCI	GGATG	8 cut(s)	105, 397, 795, 1017, 1660, 1726, 2109, 2123
BtsIMutI	CAGTG	2 cut(s)	1192, 1622
BveI	ACCTGC	1 cut(s)	2038
Cac8I	GCNNGC	5 cut(s)	505, 556, 1116, 1357, 1766
Cfr13I	GGNCC	3 cut(s)	1200, 1201, 1549
Cfr42I	CCGCGG	1 cut(s)	993
Csp6I	GTAC	8 cut(s)	457, 627, 935, 941, 1448, 1573, 1783, 1888
CspCI	CAANNNNNGTGG	6 cut(s)	460, 495, 975, 1010, 1672, 1707
CviQI	GTAC	8 cut(s)	457, 627, 935, 941, 1448, 1573, 1783, 1888
DdeI	CTNAG	2 cut(s)	579, 1961
DpnI	GATC	5 cut(s)	1557, 1586, 1796, 2229, 2284
DpnII	GATC	5 cut(s)	1555, 1584, 1794, 2227, 2282
DraI	TTTAAA	1 cut(s)	1741
Eam1104I	CTCTTC	2 cut(s)	18, 1118
EarI	CTCTTC	2 cut(s)	18, 1118
Eco105I	TACGTA	1 cut(s)	1782
Eco130I	CCWWGG	1 cut(s)	2377
Eco24I	GRGCYC	1 cut(s)	1204
Eco57I	CTGAAG	2 cut(s)	204, 2415
EcoNI	CCTNNNNNAGG	1 cut(s)	1511
EcoO109I	RGGNCCY	3 cut(s)	1200, 1201, 1549
EcoRII	CCWGG	3 cut(s)	558, 1203, 1558
EcoT14I	CCWWGG	1 cut(s)	2377
EcoT22I	ATGCAT	2 cut(s)	1309, 1462
EcoT38I	GRGCYC	1 cut(s)	1204
ErhI	CCWWGG	1 cut(s)	2377
FalI	AAGNNNNNCTT	6 cut(s)	1386, 1418, 1911, 1943, 2388, 2420
FaqI	GGGAC	2 cut(s)	127, 1334
FauNDI	CATATG	1 cut(s)	418
FbaI	TGATCA	2 cut(s)	1584, 1794
FblI	GTMKAC	1 cut(s)	1467
FokI	GGATG	8 cut(s)	92, 404, 802, 1024, 1647, 1733, 2116, 2130
FriOI	GRGCYC	1 cut(s)	1204
FspBI	CTAG	5 cut(s)	92, 963, 1553, 1730, 2231
HaeIII	GGCC	4 cut(s)	1202, 1383, 1551, 1768
HapII	CCGG	4 cut(s)	109, 256, 1034, 1318
HincII	GTYRAC	1 cut(s)	1536
HindII	GTYRAC	1 cut(s)	1536
HindIII	AAGCTT	1 cut(s)	308
HinfI	GANTC	9 cut(s)	10, 112, 409, 425, 1270, 1654, 1820, 2057, 2234
HpaI	GTTAAC	1 cut(s)	1536
HpaII	CCGG	4 cut(s)	109, 256, 1034, 1318
HphI	GGTGA	6 cut(s)	63, 605, 611, 652, 779, 1762
Hpy166II	GTNNAC	6 cut(s)	1468, 1536, 1674, 1785, 1925, 2016
Hpy188III	TCNNGA	9 cut(s)	85, 109, 304, 449, 1664, 2063, 2087, 2231, 2447
Hpy8I	GTNNAC	6 cut(s)	1468, 1536, 1674, 1785, 1925, 2016
HpyCH4IV	ACGT	2 cut(s)	1781, 1886
HpyF10VI	GCNNNNNNNGC	6 cut(s)	523, 829, 1262, 1937, 2033, 2405
HpyF3I	CTNAG	2 cut(s)	579, 1961
HpySE526I	ACGT	2 cut(s)	1781, 1886
Kpn2I	TCCGGA	1 cut(s)	108
Ksp22I	TGATCA	2 cut(s)	1584, 1794
KspAI	GTTAAC	1 cut(s)	1536
KspI	CCGCGG	1 cut(s)	993
Kzo9I	GATC	5 cut(s)	1555, 1584, 1794, 2227, 2282
LmnI	GCTCC	5 cut(s)	136, 842, 1262, 1829, 1870
Lsp1109I	GCAGC	8 cut(s)	257, 339, 1407, 1711, 1720, 1949, 2020, 2173
MaeI	CTAG	5 cut(s)	92, 963, 1553, 1730, 2231
MaeII	ACGT	2 cut(s)	1781, 1886
MaeIII	GTNAC	3 cut(s)	1235, 1882, 1987
MalI	GATC	5 cut(s)	1557, 1586, 1796, 2229, 2284
MboI	GATC	5 cut(s)	1555, 1584, 1794, 2227, 2282
MfeI	CAATTG	1 cut(s)	1539
MflI	RGATCY	1 cut(s)	1555
MhlI	GDGCHC	1 cut(s)	1204
MlyI	GAGTC	1 cut(s)	4
MmeI	TCCRAC	1 cut(s)	348
Mph1103I	ATGCAT	2 cut(s)	1309, 1462
MroI	TCCGGA	1 cut(s)	108
MseI	TTAA	9 cut(s)	519, 795, 852, 1062, 1490, 1535, 1740, 2199, 2313
MslI	CAYNNNNRTG	3 cut(s)	527, 1278, 1665
MspA1I	CMGCKG	1 cut(s)	992
MspI	CCGG	4 cut(s)	109, 256, 1034, 1318
MspR9I	CCNGG	4 cut(s)	560, 1205, 1319, 1560
MssI	GTTTAAAC	1 cut(s)	1741
MunI	CAATTG	1 cut(s)	1539
MvaI	CCWGG	3 cut(s)	560, 1205, 1560
MvnI	CGCG	2 cut(s)	992, 2356
MwoI	GCNNNNNNNGC	6 cut(s)	523, 829, 1262, 1937, 2033, 2405
NciI	CCSGG	1 cut(s)	1319
NcoI	CCATGG	1 cut(s)	2377
NdeI	CATATG	1 cut(s)	418
NdeII	GATC	5 cut(s)	1555, 1584, 1794, 2227, 2282
NlaIV	GGNNCC	4 cut(s)	1137, 1202, 1505, 1872
NmeAIII	GCCGAG	2 cut(s)	247, 408
NmuCI	GTSAC	3 cut(s)	1235, 1882, 1987
NsiI	ATGCAT	2 cut(s)	1309, 1462
NspI	RCATGY	4 cut(s)	507, 608, 1118, 1283
OliI	CACNNNNGTG	1 cut(s)	527
PaeI	GCATGC	2 cut(s)	507, 1118
PaqCI	CACCTGC	1 cut(s)	2038
PciI	ACATGT	1 cut(s)	1279
PfeI	GAWTC	8 cut(s)	112, 409, 425, 1270, 1654, 1820, 2057, 2234
PfoI	TCCNGGA	1 cut(s)	1317
PleI	GAGTC	1 cut(s)	4
PmeI	GTTTAAAC	1 cut(s)	1741
PpsI	GAGTC	1 cut(s)	4
Ppu21I	YACGTR	2 cut(s)	1782, 1887
PscI	ACATGT	1 cut(s)	1279
PshBI	ATTAAT	2 cut(s)	852, 1062
Psp6I	CCWGG	3 cut(s)	558, 1203, 1558
PspGI	CCWGG	3 cut(s)	558, 1203, 1558
PspN4I	GGNNCC	4 cut(s)	1137, 1202, 1505, 1872
PspOMI	GGGCCC	1 cut(s)	1200
PspPI	GGNCC	3 cut(s)	1200, 1201, 1549
PstI	CTGCAG	1 cut(s)	357
PsuI	RGATCY	1 cut(s)	1555
RsaI	GTAC	8 cut(s)	458, 628, 936, 942, 1449, 1574, 1784, 1889
RsaNI	GTAC	8 cut(s)	457, 627, 935, 941, 1448, 1573, 1783, 1888
RseI	CAYNNNNRTG	3 cut(s)	527, 1278, 1665
SacII	CCGCGG	1 cut(s)	993
SaqAI	TTAA	9 cut(s)	519, 795, 852, 1062, 1490, 1535, 1740, 2199, 2313
Sau3AI	GATC	5 cut(s)	1555, 1584, 1794, 2227, 2282
Sau96I	GGNCC	3 cut(s)	1200, 1201, 1549
ScaI	AGTACT	1 cut(s)	628
SchI	GAGTC	1 cut(s)	4
ScrFI	CCNGG	4 cut(s)	560, 1205, 1319, 1560
SduI	GDGCHC	1 cut(s)	1204
SfcI	CTRYAG	6 cut(s)	353, 622, 951, 1002, 1109, 1195
Sfr303I	CCGCGG	1 cut(s)	993
SgrBI	CCGCGG	1 cut(s)	993
SmiMI	CAYNNNNRTG	3 cut(s)	527, 1278, 1665
SmlI	CTYRAG	2 cut(s)	1508, 2061
SmoI	CTYRAG	2 cut(s)	1508, 2061
SnaBI	TACGTA	1 cut(s)	1782
SphI	GCATGC	2 cut(s)	507, 1118
SsiI	CCGC	8 cut(s)	43, 335, 422, 990, 992, 1097, 1810, 2249
SspI	AATATT	1 cut(s)	1054
SspMI	CTAG	5 cut(s)	92, 963, 1553, 1730, 2231
StyD4I	CCNGG	4 cut(s)	558, 1203, 1317, 1558
StyI	CCWWGG	1 cut(s)	2377
TaiI	ACGT	2 cut(s)	1784, 1889
TaqI	TCGA	3 cut(s)	147, 1530, 1635
TatI	WGTACW	4 cut(s)	456, 626, 934, 1447
TauI	GCSGC	3 cut(s)	45, 1812, 2252
TfiI	GAWTC	8 cut(s)	112, 409, 425, 1270, 1654, 1820, 2057, 2234
Tru1I	TTAA	9 cut(s)	519, 795, 852, 1062, 1490, 1535, 1740, 2199, 2313
Tru9I	TTAA	9 cut(s)	519, 795, 852, 1062, 1490, 1535, 1740, 2199, 2313
TscAI	CASTG	2 cut(s)	1199, 1629
TseFI	GTSAC	3 cut(s)	1235, 1882, 1987
TseI	GCWGC	8 cut(s)	270, 352, 1420, 1708, 1724, 1937, 2033, 2186
Tsp45I	GTSAC	3 cut(s)	1235, 1882, 1987
TspDTI	ATGAA	9 cut(s)	377, 585, 923, 1059, 1136, 1262, 1628, 1658, 2070
TspRI	CASTG	2 cut(s)	1199, 1629
VspI	ATTAAT	2 cut(s)	852, 1062
XagI	CCTNNNNNAGG	1 cut(s)	1511
XapI	RAATTY	1 cut(s)	1601
XbaI	TCTAGA	1 cut(s)	2230
XceI	RCATGY	4 cut(s)	507, 608, 1118, 1283
XcmI	CCANNNNNNNNNTGG	1 cut(s)	223
XmiI	GTMKAC	1 cut(s)	1467
XspI	CTAG	5 cut(s)	92, 963, 1553, 1730, 2231
ZrmI	AGTACT	1 cut(s)	628
Zsp2I	ATGCAT	2 cut(s)	1309, 1462

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.