Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0010927

Physical Location & Seq

Genomic Coordinates Reverse (-)

32656225 .. 32660693

Gene Structure 4469 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00108610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3210 bp

ATGTCTAAGACTCTTCTCTCTCGCATCAAACCCCTTCACAGCCGCAAACCCACTTGTCCATCTTCCTATTCACCACCCCACGTCAAGAAGCTAGTCTATGACACCATCCGGATTCTCACAACAGATGATAAATGGGAGCAATCCCTCGAAACCGAATTCTCCGAAACCGAAACGCTTGTTTCTGAAGTTGCCCATTTCGTTTTGGACAGAATACCCAACGCAGAGTTGGGTTTGAAGTTCTTTGACTGGGCTTTCCGGCGACCCTATTGCTGCTCTCCAAATGGGTTGGCGTATTCTTCTCTCTTGAAGCTTTTGGCAAGGTTTGGAGTGGTTGCGGAGATTGAGTTGGTCTTGCAGAGCATGAAGTTGGAACAAGTAAAGCCGAGTAAGGATGCTCTGAGCTTTGTGATTCGAGTTTACGCGGATTCTGGGTTCGTCGATAAGGCTCTTGAATTGTATAGTTTTGGGGTGAAGGTGTGTGGTTTTGTTCCGAGTGTTTTCGCATGCAATTCCTTGCTTAACACGCTTGTGAAGATTGGGAGAGTTGATGTTGCACGCCAGGTGTATGATGAAATGTCTGAGAAAGGTGGTGGGGATGGTGAGCATGTGTGTATAGATAACTACAGTACTTGTATAATGGTGAGGGGGTTGTGTAAGGAAGGGAAGGTTGAAGAAGGAAGAAGGTTGATTGAGGAGAGGTGGGGAGAGAATTGTGTGCCGAATGTTGTGTTCTACAATACGCTTATTGATGGATATTGTAAGAGAGGTGAGGTTGAAAATGCTAATGGGATGTTTAAGGAGTTGAAATTGAAGGGGTTTCTGCCTACATTGCAAACCTATGGAGCTTTGATTAATGGGTATTGCAAAGTAGGGAACTTTGTAGCGATTGATAGGCTTTTGATGGAAATGAAGGATAGAGGTTTGAGGGTCAATGTACAGGTACATAATACTATAGTTGAAGCTAGATGTAAGCACGATAGCTGTGCAACCGCGGTTGATACTGTAGCAAGGATGATTGAGAGTGGTTGTGAGCCGGATATTACAACCTATAATATTTTGATTAATAGTTCATGTAAGAAAGGGAAGGTTGAGGAAGCGGAGGAGTTTCTACAGCATGCAATGAAGAGGGGATTGGTTCCGAATGAGTTTAGTTATACTCCTGTTTTTCAAGTCTATTGCAGACGAGGGGAACACCGTAGGGCCCTGGATTTGCTTGTTGAGATGACAAAAAGAGGTCACAAACCTGATTTGGTTGCCTTTGGAGCTTTGATTCATGGACATGTTGTTTCTGGGGAAGTTGATTATGCATTGACAGTTCGGGACAGAATGATAGAAAATGGAATACTCCCCGATGCTTGCATTTACAATGTGTTGATGAGTGGCCTTTGCAAGAAAGGGAGGCTTCCTACTGCCAAACTGCTGCTTGGTCAGATGCTTGACCAAAATGTACTACCTGATGCATTTGTCTACGCCACATTGGTAGATGGGTTAATCAGAAATGGGAACCTTGAGGAGGCTAAAAATATATTCGAGTTAACAATTGAAAAGGGCCTAGATCCTGGTGTTGTGGGGTACAATGCTATGATCAAGGGTTTCTGCAAATTTGGAACAATGAATGATGCACTGTCATTCCTCGAAAAGATGAAGAAAAGGAATCATCATCCTGATGGGTTTACTTACTCCACAATAATTGATGGGTATGTCAAGCAGCATAATTTGGATGCTGCACTAGAAATGTTTAAACAGATGGTGAAACAGAGATGCAGGCCAAATGTCATTACATACACCTCCTTGATCAATGGGTTTTGCCGCAAAGGAGATTCTGATGGAGCTGTAAAAACTTTCGTAGAGATGCAATCTTGTGGTGTGGAGCCAAATATAGTCACGTACAGCATACTTATTGGAAACTTTTGCAAGCAAGGTAAACTTGCAAAAGCAGCCTCCTTTTTTGAACTAATGCTGAGGAAAAAGTGCAGTCCTAATGATGTCACTATCCATTATTTGCTAAATGGGTTTACAAACATTGCACCTGCTGCAATTTCAAGGGAAGTGAATGAATCTCAAGAAAAGGAGAAATCTATTTTTCTTGATTTATATAAAAGGATGATAGCCGATGGATGGTTTCAGAAGTCTGCTGTCTATAATTCCATTCTCATTTGTCTTTGTCAGTATGGAATGGTTGAAACTGCATTACAGTTTAACAGTAAGTTTAGAAGTAGAGGTATTGATCTAGATTCTGTTTCTTTTGCGGCTGTGCTACATGGTCTTTTCTTGGGAGGAAGATCAAAGGACTGGAAGAATATCATTTATTTTAACTTGAAGGAAAAAGAACTCCAAACTGCTGTCAAATACTCGCATATAATAGATGCACAGTTCCATGGAGGAAGAATATCTGAAGCTACACTTGCTTTACAGTCATTAGTTGAAGATGATAAGCCTCAACATCAAGAACTCTCAGATGTGACTGTGGTCTTGTTTCTGCTAGTTGCATCTGGTATGGATCCTTTGGACCTCCGCTTTTGTTGGTTGCCAGGTAAGGAAGAAGTATGGGCAATATTACTGAAATGCAGTGAGCGGTTCAACAGAGCTTTGACGGAGCTAGACAAGTTCGAGATTGAGGAGGCAGTGGCCCCGGAATTGCAGACAATTAAGCAAATGGTGGAAGATGGTTGCGCTGAGAATGCAATTTCTTTGCTGATCTTGGCCAGTAGCATTCTAGGCAAGGTTCTCAAGTACTGCAAGAAGCACATAGATGACAAGGAGGGTGATCCTAAGACTTGGGACACCAATTACATAGAGACTGTGGCGAACATGATCAAGGGAATGATTCCTAGAGAGATTCACAAGACTTTCAACATCAAGAATGACTTCACTCCTGAGGGAAGAAGGTTCCTAAGGAGAACCAATAGTTATTTCATTGTCGGGCATGAGTCTCTTTGCTACTTGCTCGTCGTTGTTCGCCGCCCTCTGGGCGTCGGTGTATTGGGTCCAATATTTCGCGACGGTGGGGAACGGCGTCAGGAGCTGCTCGTAGATCGGCGCAGCCTCCGAGATCTGCAAACGGCGGGTCTGATTGGCGTGGTGGCTGGCCATTGTATTTGTGATTATGGTGAGCCACTTTCCACGGATCGGAGTAAAAGGAGTAGAGAGAGTGAGAGAGTTTTGGAAGAGGAGTGGGAGGTTGCTGCCGAAGCAGATAGGATTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

His_Phos_1 PPR PPR_1 PPR_2 PPR_3 p450

Protein Analysis

1069

Amino Acids

120.52

Weight (kDa)

7.24

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	164 - 219	1.2e-07	PPR repeat family
PPR_3	PF13812	237 - 287	2.5e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	237 - 268	5.6e-11	PPR repeat
PPR_2	PF13041	240 - 289	1.3e-16	PPR repeat family
PPR	PF01535	243 - 273	1.9e-07	PPR repeat
PPR_2	PF13041	279 - 324	2e-07	PPR repeat family
PPR_3	PF13812	298 - 355	2e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	311 - 359	2.8e-08	PPR repeat family
PPR_1	PF12854	342 - 371	1.3e-11	PPR repeat
PPR_2	PF13041	345 - 386	1.1e-10	PPR repeat family
PPR_long	PF17177	367 - 467	8.3e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	384 - 427	3.6e-08	PPR repeat family
PPR_3	PF13812	404 - 460	8.1e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	446 - 478	4.1e-07	PPR repeat
PPR_2	PF13041	450 - 498	5.8e-10	PPR repeat family
PPR_3	PF13812	474 - 532	1.1e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	516 - 549	7.9e-09	PPR repeat
PPR_2	PF13041	520 - 569	8.6e-17	PPR repeat family
PPR	PF01535	525 - 552	2.7e-06	PPR repeat
PPR_3	PF13812	545 - 601	1.4e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	552 - 584	3.3e-07	PPR repeat
PPR_2	PF13041	558 - 604	8.3e-17	PPR repeat family
PPR	PF01535	558 - 588	3.7e-06	PPR repeat
PPR_long	PF17177	569 - 670	6.5e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	588 - 619	4.8e-12	PPR repeat
PPR_3	PF13812	590 - 637	2e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	590 - 639	3.2e-18	PPR repeat family
PPR	PF01535	593 - 623	3.7e-08	PPR repeat
PPR_3	PF13812	616 - 672	1.6e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	622 - 653	3.6e-09	PPR repeat
PPR_2	PF13041	628 - 673	7.1e-11	PPR repeat family
Skp1_POZ	PF03931	869 - 916	9.3e-13	Skp1 family, tetramerisation domain
Skp1	PF01466	933 - 969	3.2e-10	Skp1 family, dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010927)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52620
fragaria_vesca	FvH4_6g30030
malus_domestica	MD17G1208200.v1.1
prunus_persica	Prupe.3G082900_v2.0.a1
pyrus_communis	pycom17g21100
rosa_chinensis	RchiOBHm_Chr2g0136061 RchiOBHm_Chr2g0136071
rosa_laevigata	RLG00000019500
rosa_multiflora	Rmu_sc0002070.1_g000001 Rmu_sc0002070.1_g000002 Rmu_sc0002986.1_g000008 Rmu_sc0002986.1_g000009
rosa_roxburghii	Rroxscaffold_2G00108610
rosa_rugosa	Rorug02G0327800
rosa_samantha	Rh2AG379700 Rh2BG386200 Rh2CG365600 Rh2CG365700 Rh2DG402500
rosa_wichuraiana	Rw2G030950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2038
Acc36I	ACCTGC	1 cut(s)	2038
AccBSI	CCGCTC	1 cut(s)	2575
AccI	GTMKAC	1 cut(s)	1467
AccII	CGCG	3 cut(s)	422, 992, 3000
AccIII	TCCGGA	1 cut(s)	108
AclWI	GGATC	5 cut(s)	1550, 2495, 2508, 2762, 3135
AcoI	YGGCCR	2 cut(s)	2703, 3088
AcsI	RAATTY	2 cut(s)	155, 1601
AcuI	CTGAAG	2 cut(s)	204, 2415
AcyI	GRCGYC	2 cut(s)	2973, 3016
AfaI	GTAC	7 cut(s)	628, 936, 942, 1449, 1574, 1889, 2735
AfiI	CCNNNNNNNGG	4 cut(s)	1513, 2118, 2968, 3129
AflIII	ACRYGT	1 cut(s)	1279
AjiI	CACGTC	1 cut(s)	82
AjnI	CCWGG	4 cut(s)	558, 1203, 1558, 2530
AjuI	GAANNNNNNNTTGG	4 cut(s)	1115, 1147, 2897, 2929
AleI	CACNNNNGTG	1 cut(s)	527
AloI	GAACNNNNNNTCC	2 cut(s)	416, 448
Alw26I	GTCTC	2 cut(s)	2792, 2937
AlwI	GGATC	5 cut(s)	1550, 2495, 2508, 2762, 3135
AlwNI	CAGNNNCTG	1 cut(s)	3025
Aor13HI	TCCGGA	1 cut(s)	108
AoxI	GGCC	7 cut(s)	1200, 1381, 1549, 1766, 2628, 2703, 3088
ApaI	GGGCCC	1 cut(s)	1204
ApeKI	GCWGC	9 cut(s)	270, 1420, 1708, 1724, 1937, 2033, 3025, 3042, 3185
ApoI	RAATTY	2 cut(s)	155, 1601
ArsI	GACNNNNNNTTYG	2 cut(s)	3052, 3084
AseI	ATTAAT	2 cut(s)	852, 1062
Asp700I	GAANNNNTTC	1 cut(s)	2867
AspLEI	GCGC	2 cut(s)	2675, 3042
AspS9I	GGNCC	6 cut(s)	1200, 1201, 1549, 2509, 2629, 2987
AsuC2I	CCSGG	1 cut(s)	2633
AsuHPI	GGTGA	8 cut(s)	63, 481, 611, 652, 779, 1762, 2777, 3122
AvaII	GGWCC	2 cut(s)	2509, 2987
AxyI	CCTNAGG	2 cut(s)	2877, 2894
BaeGI	GKGCMC	1 cut(s)	1204
BalI	TGGCCA	2 cut(s)	2705, 3090
BamHI	GGATCC	1 cut(s)	2500
BanII	GRGCYC	1 cut(s)	1204
BbvCI	CCTCAGC	1 cut(s)	1961
BbvI	GCAGC	9 cut(s)	257, 1407, 1711, 1720, 1949, 2020, 3012, 3054, 3172
BceAI	ACGGC	2 cut(s)	3029, 3078
BcgI	CGANNNNNNTGC	4 cut(s)	249, 283, 965, 999
BciT130I	CCWGG	4 cut(s)	560, 1205, 1560, 2532
BclI	TGATCA	3 cut(s)	1584, 1794, 2814
BcnI	CCSGG	1 cut(s)	2633
BcoDI	GTCTC	2 cut(s)	2792, 2937
BfaI	CTAG	9 cut(s)	92, 963, 1553, 1730, 2231, 2483, 2600, 2717, 2832
BfmI	CTRYAG	4 cut(s)	622, 951, 1002, 1109
BfuAI	ACCTGC	1 cut(s)	2038
BglI	GCCNNNNNGGC	1 cut(s)	2970
BglII	AGATCT	1 cut(s)	3052
BmcAI	AGTACT	2 cut(s)	628, 2735
Bme1390I	CCNGG	5 cut(s)	560, 1205, 1560, 2532, 2633
Bme18I	GGWCC	2 cut(s)	2509, 2987
BmgBI	CACGTC	1 cut(s)	82
BmgT120I	GGNCC	6 cut(s)	1200, 1201, 1549, 2509, 2629, 2987
BmiI	GGNNCC	8 cut(s)	1137, 1202, 1505, 1872, 2502, 2631, 2891, 2988
BmrFI	CCNGG	5 cut(s)	560, 1205, 1560, 2532, 2633
BmrI	ACTGGG	1 cut(s)	256
BmuI	ACTGGG	1 cut(s)	256
BoxI	GACNNNNGTC	1 cut(s)	2468
Bpu10I	CCTNAGC	1 cut(s)	1961
BpuEI	CTTGAG	3 cut(s)	1529, 2046, 2714
BpuMI	CCSGG	1 cut(s)	2633
BsaAI	YACGTR	1 cut(s)	1887
BsaHI	GRCGYC	2 cut(s)	2973, 3016
BsaJI	CCNNGG	5 cut(s)	990, 1203, 2377, 2631, 3123
BsaWI	WCCGGW	1 cut(s)	108
BsaXI	ACNNNNNCTCC	2 cut(s)	329, 359
Bsc4I	CCNNNNNNNGG	4 cut(s)	1513, 2118, 2968, 3129
Bse1I	ACTGG	3 cut(s)	251, 2297, 2706
Bse21I	CCTNAGG	2 cut(s)	2877, 2894
Bse3DI	GCAATG	3 cut(s)	827, 1125, 2022
BseAI	TCCGGA	1 cut(s)	108
BseBI	CCWGG	4 cut(s)	560, 1205, 1560, 2532
BseDI	CCNNGG	5 cut(s)	990, 1203, 2377, 2631, 3123
BseGI	GGATG	9 cut(s)	105, 397, 601, 795, 1017, 1660, 1726, 2109, 2123
BseLI	CCNNNNNNNGG	4 cut(s)	1513, 2118, 2968, 3129
BseMI	GCAATG	3 cut(s)	827, 1125, 2022
BseMII	CTCAG	6 cut(s)	389, 570, 1952, 2469, 2667, 2868
BseNI	ACTGG	3 cut(s)	251, 2297, 2706
BseRI	GAGGAG	5 cut(s)	707, 1115, 1526, 2633, 3185
BseSI	GKGCMC	1 cut(s)	1204
BseXI	GCAGC	9 cut(s)	257, 1407, 1711, 1720, 1949, 2020, 3012, 3054, 3172
BsgI	GTGCAG	2 cut(s)	1710, 1993
Bsh1236I	CGCG	3 cut(s)	422, 992, 3000
BshFI	GGCC	7 cut(s)	1202, 1383, 1551, 1768, 2630, 2705, 3090
BsiSI	CCGG	4 cut(s)	109, 256, 1034, 2633
BslFI	GGGAC	2 cut(s)	1334, 2795
BslI	CCNNNNNNNGG	4 cut(s)	1513, 2118, 2968, 3129
BsmAI	GTCTC	2 cut(s)	2792, 2937
BsmFI	GGGAC	2 cut(s)	1334, 2795
BsmI	GAATGC	2 cut(s)	2686, 2712
BsnI	GGCC	7 cut(s)	1202, 1383, 1551, 1768, 2630, 2705, 3090
Bsp120I	GGGCCC	1 cut(s)	1200
Bsp1286I	GDGCHC	1 cut(s)	1204
Bsp13I	TCCGGA	1 cut(s)	108
Bsp1407I	TGTACA	1 cut(s)	934
Bsp19I	CCATGG	1 cut(s)	2377
Bsp68I	TCGCGA	1 cut(s)	3000
BspANI	GGCC	7 cut(s)	1202, 1383, 1551, 1768, 2630, 2705, 3090
BspCNI	CTCAG	6 cut(s)	390, 571, 1953, 2468, 2668, 2869
BspEI	TCCGGA	1 cut(s)	108
BspFNI	CGCG	3 cut(s)	422, 992, 3000
BspLI	GGNNCC	8 cut(s)	1137, 1202, 1505, 1872, 2502, 2631, 2891, 2988
BspMI	ACCTGC	1 cut(s)	2038
BspPI	GGATC	5 cut(s)	1550, 2495, 2508, 2762, 3135
BsrBI	CCGCTC	1 cut(s)	2575
BsrDI	GCAATG	3 cut(s)	827, 1125, 2022
BsrGI	TGTACA	1 cut(s)	934
BsrI	ACTGG	3 cut(s)	251, 2297, 2706
BssECI	CCNNGG	5 cut(s)	990, 1203, 2377, 2631, 3123
BssNI	GRCGYC	2 cut(s)	2973, 3016
BssT1I	CCWWGG	1 cut(s)	2377
Bst2UI	CCWGG	4 cut(s)	560, 1205, 1560, 2532
Bst6I	CTCTTC	3 cut(s)	18, 1118, 3162
BstACI	GRCGYC	2 cut(s)	2973, 3016
BstAPI	GCANNNNNTGC	1 cut(s)	2033
BstAUI	TGTACA	1 cut(s)	934
BstBAI	YACGTR	1 cut(s)	1887
BstC8I	GCNNGC	7 cut(s)	505, 556, 1116, 1357, 1766, 1916, 3088
BstDEI	CTNAG	9 cut(s)	6, 398, 579, 1961, 2455, 2676, 2772, 2877, 2894
BstDSI	CCRYGG	3 cut(s)	990, 2377, 3123
BstENI	CCTNNNNNAGG	1 cut(s)	1511
BstF5I	GGATG	9 cut(s)	105, 397, 601, 795, 1017, 1660, 1726, 2109, 2123
BstFNI	CGCG	3 cut(s)	422, 992, 3000
BstHHI	GCGC	2 cut(s)	2675, 3042
BstMAI	GTCTC	2 cut(s)	2792, 2937
BstNI	CCWGG	4 cut(s)	560, 1205, 1560, 2532
BstNSI	RCATGY	4 cut(s)	507, 608, 1118, 1283
BstPAI	GACNNNNGTC	1 cut(s)	2468
BstSCI	CCNGG	5 cut(s)	558, 1203, 1558, 2530, 2631
BstSFI	CTRYAG	4 cut(s)	622, 951, 1002, 1109
BstSLI	GKGCMC	1 cut(s)	1204
BstUI	CGCG	3 cut(s)	422, 992, 3000
BstV1I	GCAGC	9 cut(s)	257, 1407, 1711, 1720, 1949, 2020, 3012, 3054, 3172
BstX2I	RGATCY	3 cut(s)	1555, 2500, 3052
BstYI	RGATCY	3 cut(s)	1555, 2500, 3052
Bsu36I	CCTNAGG	2 cut(s)	2877, 2894
BsuRI	GGCC	7 cut(s)	1202, 1383, 1551, 1768, 2630, 2705, 3090
BtgI	CCRYGG	3 cut(s)	990, 2377, 3123
BtrI	CACGTC	1 cut(s)	82
BtsCI	GGATG	9 cut(s)	105, 397, 601, 795, 1017, 1660, 1726, 2109, 2123
BtsI	GCAGTG	2 cut(s)	2575, 2631
BtsIMutI	CAGTG	3 cut(s)	1622, 2575, 2631
BtuMI	TCGCGA	1 cut(s)	3000
BveI	ACCTGC	1 cut(s)	2038
Cac8I	GCNNGC	7 cut(s)	505, 556, 1116, 1357, 1766, 1916, 3088
CaiI	CAGNNNCTG	1 cut(s)	3025
CfoI	GCGC	2 cut(s)	2675, 3042
Cfr13I	GGNCC	6 cut(s)	1200, 1201, 1549, 2509, 2629, 2987
Cfr42I	CCGCGG	1 cut(s)	993
CseI	GACGC	2 cut(s)	2962, 3005
Csp6I	GTAC	7 cut(s)	627, 935, 941, 1448, 1573, 1888, 2734
CspCI	CAANNNNNGTGG	2 cut(s)	1672, 1707
CviQI	GTAC	7 cut(s)	627, 935, 941, 1448, 1573, 1888, 2734
DdeI	CTNAG	9 cut(s)	6, 398, 579, 1961, 2455, 2676, 2772, 2877, 2894
DraI	TTTAAA	1 cut(s)	1741
EaeI	YGGCCR	2 cut(s)	2703, 3088
Eam1104I	CTCTTC	3 cut(s)	18, 1118, 3162
EarI	CTCTTC	3 cut(s)	18, 1118, 3162
Eco130I	CCWWGG	1 cut(s)	2377
Eco24I	GRGCYC	1 cut(s)	1204
Eco47I	GGWCC	2 cut(s)	2509, 2987
Eco57I	CTGAAG	2 cut(s)	204, 2415
Eco81I	CCTNAGG	2 cut(s)	2877, 2894
EcoNI	CCTNNNNNAGG	1 cut(s)	1511
EcoO109I	RGGNCCY	3 cut(s)	1200, 1201, 1549
EcoRI	GAATTC	1 cut(s)	155
EcoRII	CCWGG	4 cut(s)	558, 1203, 1558, 2530
EcoT14I	CCWWGG	1 cut(s)	2377
EcoT22I	ATGCAT	2 cut(s)	1309, 1462
EcoT38I	GRGCYC	1 cut(s)	1204
ErhI	CCWWGG	1 cut(s)	2377
FalI	AAGNNNNNCTT	8 cut(s)	1386, 1418, 1911, 1943, 2388, 2420, 2852, 2884
FaqI	GGGAC	2 cut(s)	1334, 2795
FauI	CCCGC	1 cut(s)	3058
FbaI	TGATCA	3 cut(s)	1584, 1794, 2814
FblI	GTMKAC	1 cut(s)	1467
FokI	GGATG	9 cut(s)	92, 404, 608, 802, 1024, 1647, 1733, 2116, 2130
FriOI	GRGCYC	1 cut(s)	1204
FspBI	CTAG	9 cut(s)	92, 963, 1553, 1730, 2231, 2483, 2600, 2717, 2832
GlaI	GCGC	2 cut(s)	2674, 3041
HaeIII	GGCC	7 cut(s)	1202, 1383, 1551, 1768, 2630, 2705, 3090
HapII	CCGG	4 cut(s)	109, 256, 1034, 2633
HgaI	GACGC	2 cut(s)	2962, 3005
HhaI	GCGC	2 cut(s)	2675, 3042
Hin1I	GRCGYC	2 cut(s)	2973, 3016
Hin6I	GCGC	2 cut(s)	2673, 3040
HinP1I	GCGC	2 cut(s)	2673, 3040
HincII	GTYRAC	1 cut(s)	1536
HindII	GTYRAC	1 cut(s)	1536
HindIII	AAGCTT	1 cut(s)	308
HpaI	GTTAAC	1 cut(s)	1536
HpaII	CCGG	4 cut(s)	109, 256, 1034, 2633
HphI	GGTGA	8 cut(s)	63, 481, 611, 652, 779, 1762, 2777, 3122
Hpy166II	GTNNAC	6 cut(s)	418, 1468, 1536, 1674, 1925, 2016
Hpy8I	GTNNAC	6 cut(s)	418, 1468, 1536, 1674, 1925, 2016
Hpy99I	CGWCG	4 cut(s)	440, 2954, 2978, 3005
HpyCH4IV	ACGT	2 cut(s)	81, 1886
HpyF3I	CTNAG	9 cut(s)	6, 398, 579, 1961, 2455, 2676, 2772, 2877, 2894
HpySE526I	ACGT	2 cut(s)	81, 1886
Hsp92I	GRCGYC	2 cut(s)	2973, 3016
HspAI	GCGC	2 cut(s)	2673, 3040
Kpn2I	TCCGGA	1 cut(s)	108
Ksp22I	TGATCA	3 cut(s)	1584, 1794, 2814
KspAI	GTTAAC	1 cut(s)	1536
KspI	CCGCGG	1 cut(s)	993
LmnI	GCTCC	7 cut(s)	136, 842, 1262, 1829, 1870, 2596, 3022
Lsp1109I	GCAGC	9 cut(s)	257, 1407, 1711, 1720, 1949, 2020, 3012, 3054, 3172
MaeI	CTAG	9 cut(s)	92, 963, 1553, 1730, 2231, 2483, 2600, 2717, 2832
MaeII	ACGT	2 cut(s)	81, 1886
MaeIII	GTNAC	4 cut(s)	1235, 1882, 1987, 2461
MbiI	CCGCTC	1 cut(s)	2575
MfeI	CAATTG	1 cut(s)	1539
MflI	RGATCY	3 cut(s)	1555, 2500, 3052
MhlI	GDGCHC	1 cut(s)	1204
MlsI	TGGCCA	2 cut(s)	2705, 3090
MluNI	TGGCCA	2 cut(s)	2705, 3090
MlyI	GAGTC	2 cut(s)	4, 2939
MmeI	TCCRAC	1 cut(s)	348
Mox20I	TGGCCA	2 cut(s)	2705, 3090
Mph1103I	ATGCAT	2 cut(s)	1309, 1462
MroI	TCCGGA	1 cut(s)	108
MroXI	GAANNNNTTC	1 cut(s)	2867
MscI	TGGCCA	2 cut(s)	2705, 3090
MslI	CAYNNNNRTG	4 cut(s)	527, 1278, 1665, 2751
Msp20I	TGGCCA	2 cut(s)	2705, 3090
MspA1I	CMGCKG	1 cut(s)	992
MspI	CCGG	4 cut(s)	109, 256, 1034, 2633
MspR9I	CCNGG	5 cut(s)	560, 1205, 1560, 2532, 2633
MssI	GTTTAAAC	1 cut(s)	1741
MunI	CAATTG	1 cut(s)	1539
Mva1269I	GAATGC	2 cut(s)	2686, 2712
MvaI	CCWGG	4 cut(s)	560, 1205, 1560, 2532
MvnI	CGCG	3 cut(s)	422, 992, 3000
NciI	CCSGG	1 cut(s)	2633
NcoI	CCATGG	1 cut(s)	2377
NlaIV	GGNNCC	8 cut(s)	1137, 1202, 1505, 1872, 2502, 2631, 2891, 2988
NmeAIII	GCCGAG	1 cut(s)	408
NmuCI	GTSAC	4 cut(s)	1235, 1882, 1987, 2461
NruI	TCGCGA	1 cut(s)	3000
NsiI	ATGCAT	2 cut(s)	1309, 1462
NspI	RCATGY	4 cut(s)	507, 608, 1118, 1283
OliI	CACNNNNGTG	1 cut(s)	527
PaeI	GCATGC	2 cut(s)	507, 1118
PaqCI	CACCTGC	1 cut(s)	2038
PciI	ACATGT	1 cut(s)	1279
PctI	GAATGC	2 cut(s)	2686, 2712
PdmI	GAANNNNTTC	1 cut(s)	2867
PleI	GAGTC	2 cut(s)	4, 2938
PmeI	GTTTAAAC	1 cut(s)	1741
PpsI	GAGTC	2 cut(s)	4, 2938
Ppu21I	YACGTR	1 cut(s)	1887
PscI	ACATGT	1 cut(s)	1279
PshAI	GACNNNNGTC	1 cut(s)	2468
PshBI	ATTAAT	2 cut(s)	852, 1062
Psp6I	CCWGG	4 cut(s)	558, 1203, 1558, 2530
PspGI	CCWGG	4 cut(s)	558, 1203, 1558, 2530
PspN4I	GGNNCC	8 cut(s)	1137, 1202, 1505, 1872, 2502, 2631, 2891, 2988
PspOMI	GGGCCC	1 cut(s)	1200
PspPI	GGNCC	6 cut(s)	1200, 1201, 1549, 2509, 2629, 2987
PstNI	CAGNNNCTG	1 cut(s)	3025
PsuI	RGATCY	3 cut(s)	1555, 2500, 3052
RruI	TCGCGA	1 cut(s)	3000
RsaI	GTAC	7 cut(s)	628, 936, 942, 1449, 1574, 1889, 2735
RsaNI	GTAC	7 cut(s)	627, 935, 941, 1448, 1573, 1888, 2734
RseI	CAYNNNNRTG	4 cut(s)	527, 1278, 1665, 2751
SacII	CCGCGG	1 cut(s)	993
Sau96I	GGNCC	6 cut(s)	1200, 1201, 1549, 2509, 2629, 2987
ScaI	AGTACT	2 cut(s)	628, 2735
SchI	GAGTC	2 cut(s)	4, 2939
ScrFI	CCNGG	5 cut(s)	560, 1205, 1560, 2532, 2633
SduI	GDGCHC	1 cut(s)	1204
SfcI	CTRYAG	4 cut(s)	622, 951, 1002, 1109
Sfr303I	CCGCGG	1 cut(s)	993
SgrBI	CCGCGG	1 cut(s)	993
SinI	GGWCC	2 cut(s)	2509, 2987
SmiMI	CAYNNNNRTG	4 cut(s)	527, 1278, 1665, 2751
SmlI	CTYRAG	3 cut(s)	1508, 2061, 2729
SmoI	CTYRAG	3 cut(s)	1508, 2061, 2729
SphI	GCATGC	2 cut(s)	507, 1118
SspI	AATATT	3 cut(s)	1054, 2556, 2994
SspMI	CTAG	9 cut(s)	92, 963, 1553, 1730, 2231, 2483, 2600, 2717, 2832
StyD4I	CCNGG	5 cut(s)	558, 1203, 1558, 2530, 2631
StyI	CCWWGG	1 cut(s)	2377
TaiI	ACGT	2 cut(s)	84, 1889
TaqI	TCGA	6 cut(s)	147, 412, 438, 1530, 1635, 2610
TatI	WGTACW	4 cut(s)	626, 934, 1447, 2733
TauI	GCSGC	4 cut(s)	45, 1812, 2252, 2964
TscAI	CASTG	3 cut(s)	1629, 2575, 2631
TseFI	GTSAC	4 cut(s)	1235, 1882, 1987, 2461
TseI	GCWGC	9 cut(s)	270, 1420, 1708, 1724, 1937, 2033, 3025, 3042, 3185
Tsp45I	GTSAC	4 cut(s)	1235, 1882, 1987, 2461
TspGWI	ACGGA	2 cut(s)	2609, 3140
TspRI	CASTG	3 cut(s)	1629, 2575, 2631
VpaK11BI	GGWCC	2 cut(s)	2509, 2987
VspI	ATTAAT	2 cut(s)	852, 1062
XagI	CCTNNNNNAGG	1 cut(s)	1511
XapI	RAATTY	2 cut(s)	155, 1601
XbaI	TCTAGA	1 cut(s)	2230
XceI	RCATGY	4 cut(s)	507, 608, 1118, 1283
XcmI	CCANNNNNNNNNTGG	1 cut(s)	223
XmiI	GTMKAC	1 cut(s)	1467
XmnI	GAANNNNTTC	1 cut(s)	2867
XspI	CTAG	9 cut(s)	92, 963, 1553, 1730, 2231, 2483, 2600, 2717, 2832
ZrmI	AGTACT	2 cut(s)	628, 2735
Zsp2I	ATGCAT	2 cut(s)	1309, 1462

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.