RLG00000020579

rRNA-processing protein FCF1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016464
Physical Location & Seq
Reverse (-)
66421311 .. 66426892
5582 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020579

Sequence Viewer

Length: 810 bp
ATGTGGCAACCGCAGCAAAGGAAGAAGAAGCATAGGGTAGAGACCAGAACGACAGCCTCCACGATGGAGAGTATCTTGAAGACGACGTCGTCTATGATGCGCAGCCGCAAGTCGACGCGCTTCCACCGGAACACCGACGATAATTTCCTCGGCCACTGCTCCTTGCTGATCACCTTCTTTAACGACCACGTCTTCGTCGACGACTTGGAGCTGTTTGAGCTGCTCTTCTGCAGTCTGTCAAATACCTCTCGTAGCTCTCTGTTTTCCATGACGTCAATCCCTGTAGCTCGATCTGTATTGTTGTTGTTTTCGGGTGAGGAATATGTGTGTGTTTTCGACGAGGTTAAGAAGGTGAGACTGAGATGTTCTGCACTCTTAGGTGATCAGCAGACTCTTATATCCTTGTTAGACAAGAAGGGTCTTGCTTTCTTATGGAATGGACTTGAGGTCTTGCACCCACAGTCCATGAGACTGACTAGGGAAGATGATATTCCTGCTGTACTAACTAGCATCATATTGAACTGGAATGTGACCATGTTGGATATTGTTAGCACTCACATGCTTGGTCGATATGGTTTTCTTGCTGAGCTAGATAAGTACAAATGCTATGTTGCTGAGGTTAATGCTATAAAGGTGTCATGCTATAAAGAGGAGGTTTTGAATTCAAAGAAGAATCTCAGCAAAGAAAAGCTACCCAGAAATGTGCCAAATGTTTCTTCGCTACTGTTCTTTAAGTACAACACTGCTATGGAGATGTTTTACCGGGTTTTGATGGATACCAACTTCATCAATTTCTCTATCCAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Fcf1

Protein Analysis

270

Amino Acids

31.2

Weight (kDa)

9.08

Isoelectric Point (pI)

26.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
AatII GACGTC 2 cut(s) 89, 275
Acc16I TGCGCA 1 cut(s) 101
AccI GTMKAC 2 cut(s) 113, 198
AccII CGCG 1 cut(s) 118
AciI CCGC 2 cut(s) 11, 106
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 1 cut(s) 661
AcyI GRCGYC 2 cut(s) 86, 272
AfaI GTAC 3 cut(s) 501, 599, 737
AgsI TTSAA 4 cut(s) 79, 520, 661, 666
AhdI GACNNNNNGTC 1 cut(s) 446
AjiI CACGTC 1 cut(s) 190
AjuI GAANNNNNNNTTGG 2 cut(s) 700, 732
AluBI AGCT 6 cut(s) 211, 220, 255, 287, 589, 691
AluI AGCT 6 cut(s) 211, 220, 255, 287, 589, 691
Alw26I GTCTC 3 cut(s) 35, 349, 463
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 3 cut(s) 13, 102, 220
ApoI RAATTY 1 cut(s) 661
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AspLEI GCGC 2 cut(s) 102, 120
AsuC2I CCSGG 1 cut(s) 764
AsuHPI GGTGA 4 cut(s) 163, 326, 364, 392
BbsI GAAGAC 2 cut(s) 86, 184
BbvCI CCTCAGC 1 cut(s) 615
BbvI GCAGC 3 cut(s) 25, 114, 207
BccI CCATC 2 cut(s) 58, 766
BciVI GTATCC 1 cut(s) 769
BclI TGATCA 2 cut(s) 168, 382
BcnI CCSGG 1 cut(s) 764
BcoDI GTCTC 3 cut(s) 35, 349, 463
BfaI CTAG 3 cut(s) 477, 507, 590
BfmI CTRYAG 2 cut(s) 229, 282
BfuI GTATCC 1 cut(s) 769
BisI GCNGC 4 cut(s) 14, 103, 106, 221
BlpI GCTNAGC 1 cut(s) 585
BlsI GCNGC 4 cut(s) 15, 104, 107, 222
Bme1390I CCNGG 1 cut(s) 764
BmeRI GACNNNNNGTC 1 cut(s) 446
BmgBI CACGTC 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 764
BmsI GCATC 2 cut(s) 87, 519
BpiI GAAGAC 2 cut(s) 86, 184
Bpu10I CCTNAGC 1 cut(s) 615
Bpu1102I GCTNAGC 1 cut(s) 585
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 764
BsaHI GRCGYC 2 cut(s) 86, 272
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 148
BsaWI WCCGGW 1 cut(s) 126
Bse1I ACTGG 1 cut(s) 527
BseDI CCNNGG 1 cut(s) 148
BseMII CTCAG 4 cut(s) 350, 576, 606, 691
BseNI ACTGG 1 cut(s) 527
BseRI GAGGAG 1 cut(s) 665
BseXI GCAGC 3 cut(s) 25, 114, 207
BsgI GTGCAG 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 1 cut(s) 153
BsiSI CCGG 2 cut(s) 127, 763
BsmAI GTCTC 3 cut(s) 35, 349, 463
BsnI GGCC 1 cut(s) 153
Bso31I GGTCTC 1 cut(s) 35
Bsp143I GATC 3 cut(s) 168, 290, 382
Bsp1720I GCTNAGC 1 cut(s) 585
BspACI CCGC 2 cut(s) 11, 106
BspANI GGCC 1 cut(s) 153
BspCNI CTCAG 4 cut(s) 351, 577, 607, 690
BspFNI CGCG 1 cut(s) 118
BspMAI CTGCAG 1 cut(s) 233
BspQI GCTCTTC 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 35
BsrI ACTGG 1 cut(s) 527
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 3 cut(s) 168, 290, 382
BssNI GRCGYC 2 cut(s) 86, 272
Bst4CI ACNGT 2 cut(s) 462, 726
Bst6I CTCTTC 1 cut(s) 230
BstACI GRCGYC 2 cut(s) 86, 272
BstDEI CTNAG 5 cut(s) 359, 376, 585, 615, 677
BstFNI CGCG 1 cut(s) 118
BstHHI GCGC 2 cut(s) 102, 120
BstKTI GATC 3 cut(s) 171, 293, 385
BstMAI GTCTC 3 cut(s) 35, 349, 463
BstMBI GATC 3 cut(s) 168, 290, 382
BstMWI GCNNNNNNNGC 2 cut(s) 13, 217
BstNSI RCATGY 1 cut(s) 562
BstSCI CCNGG 1 cut(s) 762
BstSFI CTRYAG 2 cut(s) 229, 282
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 3 cut(s) 25, 114, 207
BstV2I GAAGAC 2 cut(s) 86, 184
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 1 cut(s) 153
BtrI CACGTC 1 cut(s) 190
BtsI GCAGTG 2 cut(s) 154, 741
BtsIMutI CAGTG 2 cut(s) 154, 741
CfoI GCGC 2 cut(s) 102, 120
CseI GACGC 1 cut(s) 124
Csp6I GTAC 3 cut(s) 500, 598, 736
CviAII CATG 5 cut(s) 268, 466, 535, 559, 639
CviJI RGCY 9 cut(s) 56, 105, 153, 211, 220, 255, 287, 589, 691
CviKI_1 RGCY 9 cut(s) 56, 105, 153, 211, 220, 255, 287, 589, 691
CviQI GTAC 3 cut(s) 500, 598, 736
DdeI CTNAG 5 cut(s) 359, 376, 585, 615, 677
DpnI GATC 3 cut(s) 170, 292, 384
DpnII GATC 3 cut(s) 168, 290, 382
DrdI GACNNNNNNGTC 1 cut(s) 88
DriI GACNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 230
Eam1105I GACNNNNNGTC 1 cut(s) 446
EarI CTCTTC 1 cut(s) 230
Eco31I GGTCTC 1 cut(s) 35
EcoRI GAATTC 1 cut(s) 661
FaeI CATG 5 cut(s) 271, 469, 538, 562, 642
FatI CATG 5 cut(s) 267, 465, 534, 558, 638
FbaI TGATCA 2 cut(s) 168, 382
FblI GTMKAC 2 cut(s) 113, 198
Fnu4HI GCNGC 4 cut(s) 14, 103, 106, 221
Fsp4HI GCNGC 4 cut(s) 14, 103, 106, 221
FspBI CTAG 3 cut(s) 477, 507, 590
FspI TGCGCA 1 cut(s) 101
GlaI GCGC 2 cut(s) 101, 119
GluI GCNGC 4 cut(s) 14, 103, 106, 221
HaeIII GGCC 1 cut(s) 153
HapII CCGG 2 cut(s) 127, 763
HgaI GACGC 1 cut(s) 124
HhaI GCGC 2 cut(s) 102, 120
Hin1I GRCGYC 2 cut(s) 86, 272
Hin1II CATG 5 cut(s) 271, 469, 538, 562, 642
Hin6I GCGC 2 cut(s) 100, 118
HinP1I GCGC 2 cut(s) 100, 118
HincII GTYRAC 2 cut(s) 114, 199
HindII GTYRAC 2 cut(s) 114, 199
HinfI GANTC 2 cut(s) 391, 673
HpaII CCGG 2 cut(s) 127, 763
HphI GGTGA 4 cut(s) 163, 326, 364, 392
Hpy166II GTNNAC 2 cut(s) 114, 199
Hpy188III TCNNGA 2 cut(s) 76, 802
Hpy8I GTNNAC 2 cut(s) 114, 199
Hpy99I CGWCG 7 cut(s) 88, 91, 118, 140, 200, 203, 341
HpyAV CCTTC 3 cut(s) 184, 343, 409
HpyCH4III ACNGT 2 cut(s) 462, 726
HpyCH4IV ACGT 3 cut(s) 86, 189, 272
HpyCH4V TGCA 3 cut(s) 231, 371, 454
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 217
HpyF3I CTNAG 5 cut(s) 359, 376, 585, 615, 677
HpySE526I ACGT 3 cut(s) 86, 189, 272
Hsp92I GRCGYC 2 cut(s) 86, 272
Hsp92II CATG 5 cut(s) 271, 469, 538, 562, 642
HspAI GCGC 2 cut(s) 100, 118
Ksp22I TGATCA 2 cut(s) 168, 382
Kzo9I GATC 3 cut(s) 168, 290, 382
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 2 cut(s) 164, 208
LpnPI CCDG 7 cut(s) 58, 140, 294, 507, 508, 709, 776
Lsp1109I GCAGC 3 cut(s) 25, 114, 207
LweI GCATC 2 cut(s) 87, 519
MaeI CTAG 3 cut(s) 477, 507, 590
MaeII ACGT 3 cut(s) 86, 189, 272
MaeIII GTNAC 1 cut(s) 529
MalI GATC 3 cut(s) 170, 292, 384
MboI GATC 3 cut(s) 168, 290, 382
MboII GAAGA 8 cut(s) 34, 37, 91, 184, 217, 494, 682, 708
MluCI AATT 3 cut(s) 142, 661, 790
MlyI GAGTC 1 cut(s) 385
MmeI TCCRAC 1 cut(s) 519
MseI TTAA 4 cut(s) 180, 345, 621, 732
MslI CAYNNNNRTG 2 cut(s) 557, 746
MspI CCGG 2 cut(s) 127, 763
MspR9I CCNGG 1 cut(s) 764
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 2 cut(s) 13, 217
NciI CCSGG 1 cut(s) 764
NdeII GATC 3 cut(s) 168, 290, 382
NlaIII CATG 5 cut(s) 271, 469, 538, 562, 642
NmeAIII GCCGAG 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 529
NsbI TGCGCA 1 cut(s) 101
NspI RCATGY 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 230
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 1 cut(s) 673
PflFI GACNNNGTC 3 cut(s) 85, 88, 188
PkrI GCNGC 4 cut(s) 15, 104, 107, 222
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
PstI CTGCAG 1 cut(s) 233
PsyI GACNNNGTC 3 cut(s) 85, 88, 188
RsaI GTAC 3 cut(s) 501, 599, 737
RsaNI GTAC 3 cut(s) 500, 598, 736
RseI CAYNNNNRTG 2 cut(s) 557, 746
SalI GTCGAC 2 cut(s) 112, 197
SapI GCTCTTC 1 cut(s) 230
SaqAI TTAA 4 cut(s) 180, 345, 621, 732
SatI GCNGC 4 cut(s) 14, 103, 106, 221
Sau3AI GATC 3 cut(s) 168, 290, 382
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 1 cut(s) 764
SfaNI GCATC 2 cut(s) 87, 519
SfcI CTRYAG 2 cut(s) 229, 282
SgrDI CGTCGACG 1 cut(s) 197
SmiMI CAYNNNNRTG 2 cut(s) 557, 746
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
Sse9I AATT 3 cut(s) 142, 661, 790
SsiI CCGC 2 cut(s) 11, 106
SspMI CTAG 3 cut(s) 477, 507, 590
StyD4I CCNGG 1 cut(s) 762
TaaI ACNGT 2 cut(s) 462, 726
TaiI ACGT 3 cut(s) 89, 192, 275
TaqI TCGA 5 cut(s) 113, 198, 289, 336, 568
TasI AATT 3 cut(s) 142, 661, 790
TatI WGTACW 3 cut(s) 499, 597, 735
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 1 cut(s) 673
Tru1I TTAA 4 cut(s) 180, 345, 621, 732
Tru9I TTAA 4 cut(s) 180, 345, 621, 732
TscAI CASTG 2 cut(s) 161, 748
TseFI GTSAC 1 cut(s) 529
TseI GCWGC 3 cut(s) 13, 102, 220
Tsp45I GTSAC 1 cut(s) 529
TspDTI ATGAA 1 cut(s) 775
TspRI CASTG 2 cut(s) 161, 748
Tth111I GACNNNGTC 3 cut(s) 85, 88, 188
XapI RAATTY 1 cut(s) 661
XceI RCATGY 1 cut(s) 562
XmiI GTMKAC 2 cut(s) 113, 198
XspI CTAG 3 cut(s) 477, 507, 590
ZraI GACGTC 2 cut(s) 87, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.