Rh2CG476000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0016464
Physical Location & Seq
Reverse (-)
64139404 .. 64140584
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG476000.1

Sequence Viewer

Length: 444 bp
ATGTGGCAACCGCAGCAAAGGAAGAAGAAGCATAGGGTAGAGACCAGAACGACAGCCTCCACGACGGAGAGTATCTTGAAGACGACGTCGTCTATGATGCGCAGCCGCAAGTCGACGCGCTTCCACCGGAACACCGACGATAATTTCCTCGGCCACAGCTCCTTGCTGATCACCTTCTTTAACGACCACGTCTTCGTCGACGTCGACGTCGACGACTTGGAGCTGTTTGAGCTGCTCTTCTCCAGTCTGTCAAATACCTCTCGTAGTTCTCTGTTTTCCATGACGTCAATCCCTGTAGCTCGATCTGTGTTGTTGTTGTTTTTGGGAGAGGAATGTGTGCGTGTATTCGACGAGGTTAAGAAGGTGAGACTGAGATGGTACGTGGTAGTATATATACAAATAAAGGAATTAAGAGGTTGGTGCCTGGCTTTGGTTTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

17.17

Weight (kDa)

9.51

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 88, 206
AatII GACGTC 4 cut(s) 89, 204, 210, 287
Acc16I TGCGCA 1 cut(s) 101
AccB1I GGYRCC 1 cut(s) 420
AccI GTMKAC 4 cut(s) 113, 198, 204, 210
AccII CGCG 1 cut(s) 118
AciI CCGC 2 cut(s) 11, 106
AcoI YGGCCR 1 cut(s) 151
AcyI GRCGYC 4 cut(s) 86, 201, 207, 284
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 1 cut(s) 79
AjiI CACGTC 1 cut(s) 190
AjnI CCWGG 1 cut(s) 423
AluBI AGCT 4 cut(s) 159, 223, 232, 299
AluI AGCT 4 cut(s) 159, 223, 232, 299
Alw26I GTCTC 2 cut(s) 35, 361
AoxI GGCC 1 cut(s) 151
ApeKI GCWGC 3 cut(s) 13, 102, 232
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
AspLEI GCGC 2 cut(s) 102, 120
AsuHPI GGTGA 2 cut(s) 163, 376
BanI GGYRCC 1 cut(s) 420
BbsI GAAGAC 2 cut(s) 86, 184
BbvI GCAGC 3 cut(s) 25, 114, 219
BccI CCATC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 425
BclI TGATCA 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 35, 361
BfmI CTRYAG 1 cut(s) 294
BisI GCNGC 4 cut(s) 14, 103, 106, 233
BlsI GCNGC 4 cut(s) 15, 104, 107, 234
Bme1390I CCNGG 1 cut(s) 425
BmgBI CACGTC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 422
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 1 cut(s) 87
BpiI GAAGAC 2 cut(s) 86, 184
BpmI CTGGAG 1 cut(s) 226
BsaAI YACGTR 1 cut(s) 382
BsaHI GRCGYC 4 cut(s) 86, 201, 207, 284
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 148
BsaWI WCCGGW 1 cut(s) 126
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse1I ACTGG 1 cut(s) 243
BseBI CCWGG 1 cut(s) 425
BseDI CCNNGG 1 cut(s) 148
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 1 cut(s) 243
BseXI GCAGC 3 cut(s) 25, 114, 219
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 1 cut(s) 153
BshNI GGYRCC 1 cut(s) 420
BsiSI CCGG 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 2 cut(s) 35, 361
BsnI GGCC 1 cut(s) 153
Bso31I GGTCTC 1 cut(s) 35
Bsp143I GATC 2 cut(s) 168, 302
BspACI CCGC 2 cut(s) 11, 106
BspANI GGCC 1 cut(s) 153
BspCNI CTCAG 1 cut(s) 363
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 422
BspQI GCTCTTC 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 420
BspTNI GGTCTC 1 cut(s) 35
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 2 cut(s) 168, 302
BssNI GRCGYC 4 cut(s) 86, 201, 207, 284
Bst2UI CCWGG 1 cut(s) 425
Bst6I CTCTTC 1 cut(s) 242
BstACI GRCGYC 4 cut(s) 86, 201, 207, 284
BstBAI YACGTR 1 cut(s) 382
BstDEI CTNAG 1 cut(s) 371
BstFNI CGCG 1 cut(s) 118
BstHHI GCGC 2 cut(s) 102, 120
BstKTI GATC 2 cut(s) 171, 305
BstMAI GTCTC 2 cut(s) 35, 361
BstMBI GATC 2 cut(s) 168, 302
BstMWI GCNNNNNNNGC 2 cut(s) 13, 229
BstNI CCWGG 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 423
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 3 cut(s) 25, 114, 219
BstV2I GAAGAC 2 cut(s) 86, 184
BsuRI GGCC 1 cut(s) 153
BtrI CACGTC 1 cut(s) 190
CfoI GCGC 2 cut(s) 102, 120
CseI GACGC 1 cut(s) 124
Csp6I GTAC 1 cut(s) 379
CviAII CATG 1 cut(s) 280
CviJI RGCY 8 cut(s) 56, 105, 153, 159, 223, 232, 299, 428
CviKI_1 RGCY 8 cut(s) 56, 105, 153, 159, 223, 232, 299, 428
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 1 cut(s) 371
DpnI GATC 2 cut(s) 170, 304
DpnII GATC 2 cut(s) 168, 302
DrdI GACNNNNNNGTC 2 cut(s) 88, 206
DseDI GACNNNNNNGTC 2 cut(s) 88, 206
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
Eco31I GGTCTC 1 cut(s) 35
EcoRII CCWGG 1 cut(s) 423
FaeI CATG 1 cut(s) 283
FaiI YATR 7 cut(s) 33, 95, 281, 391, 393, 395, 442
FatI CATG 1 cut(s) 279
FbaI TGATCA 1 cut(s) 168
FblI GTMKAC 4 cut(s) 113, 198, 204, 210
Fnu4HI GCNGC 4 cut(s) 14, 103, 106, 233
Fsp4HI GCNGC 4 cut(s) 14, 103, 106, 233
FspI TGCGCA 1 cut(s) 101
GlaI GCGC 2 cut(s) 101, 119
GluI GCNGC 4 cut(s) 14, 103, 106, 233
GsuI CTGGAG 1 cut(s) 226
HaeIII GGCC 1 cut(s) 153
HapII CCGG 1 cut(s) 127
HgaI GACGC 1 cut(s) 124
HhaI GCGC 2 cut(s) 102, 120
Hin1I GRCGYC 4 cut(s) 86, 201, 207, 284
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 2 cut(s) 100, 118
HinP1I GCGC 2 cut(s) 100, 118
HincII GTYRAC 4 cut(s) 114, 199, 205, 211
HindII GTYRAC 4 cut(s) 114, 199, 205, 211
HpaII CCGG 1 cut(s) 127
HphI GGTGA 2 cut(s) 163, 376
Hpy166II GTNNAC 4 cut(s) 114, 199, 205, 211
Hpy188III TCNNGA 1 cut(s) 76
Hpy8I GTNNAC 4 cut(s) 114, 199, 205, 211
HpyAV CCTTC 2 cut(s) 184, 355
HpyCH4IV ACGT 6 cut(s) 86, 189, 201, 207, 284, 381
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 229
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 6 cut(s) 86, 189, 201, 207, 284, 381
Hsp92I GRCGYC 4 cut(s) 86, 201, 207, 284
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 2 cut(s) 100, 118
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 2 cut(s) 168, 302
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 2 cut(s) 164, 220
LpnPI CCDG 6 cut(s) 58, 140, 256, 306, 410, 437
Lsp1109I GCAGC 3 cut(s) 25, 114, 219
LweI GCATC 1 cut(s) 87
MaeII ACGT 6 cut(s) 86, 189, 201, 207, 284, 381
MalI GATC 2 cut(s) 170, 304
MboI GATC 2 cut(s) 168, 302
MboII GAAGA 6 cut(s) 34, 37, 91, 184, 229, 429
MluCI AATT 2 cut(s) 142, 407
MnlI CCTC 6 cut(s) 67, 158, 268, 322, 346, 407
MseI TTAA 3 cut(s) 180, 357, 410
MspI CCGG 1 cut(s) 127
MspR9I CCNGG 1 cut(s) 425
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 2 cut(s) 13, 229
NdeII GATC 2 cut(s) 168, 302
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 1 cut(s) 422
NmeAIII GCCGAG 1 cut(s) 129
NsbI TGCGCA 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 242
PcsI WCGNNNNNNNCGW 5 cut(s) 195, 201, 204, 207, 210
PflFI GACNNNGTC 3 cut(s) 85, 88, 188
PkrI GCNGC 4 cut(s) 15, 104, 107, 234
Ppu21I YACGTR 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 423
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 1 cut(s) 422
PsyI GACNNNGTC 3 cut(s) 85, 88, 188
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SalI GTCGAC 4 cut(s) 112, 197, 203, 209
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 3 cut(s) 180, 357, 410
SatI GCNGC 4 cut(s) 14, 103, 106, 233
Sau3AI GATC 2 cut(s) 168, 302
ScrFI CCNGG 1 cut(s) 425
SfaNI GCATC 1 cut(s) 87
SfcI CTRYAG 1 cut(s) 294
SgrDI CGTCGACG 3 cut(s) 197, 203, 209
Sse9I AATT 2 cut(s) 142, 407
SsiI CCGC 2 cut(s) 11, 106
StyD4I CCNGG 1 cut(s) 423
TaiI ACGT 6 cut(s) 89, 192, 204, 210, 287, 384
TaqI TCGA 6 cut(s) 113, 198, 204, 210, 301, 348
TasI AATT 2 cut(s) 142, 407
TauI GCSGC 1 cut(s) 108
Tru1I TTAA 3 cut(s) 180, 357, 410
Tru9I TTAA 3 cut(s) 180, 357, 410
TseI GCWGC 3 cut(s) 13, 102, 232
TspDTI ATGAA 1 cut(s) 429
TspGWI ACGGA 1 cut(s) 80
Tth111I GACNNNGTC 3 cut(s) 85, 88, 188
XmiI GTMKAC 4 cut(s) 113, 198, 204, 210
ZraI GACGTC 4 cut(s) 87, 202, 208, 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.