RLG00000022164

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016010
Physical Location & Seq
Reverse (-)
81466269 .. 81467544
1276 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022164

Sequence Viewer

Length: 1119 bp
ATGGATCAGTACAAGAACTCTTCAAGCAACCAATCCCACACAAAAAACCCTTTACATATCAAAGAAAAAGAACTTGGAGAGCTTCCTTCCATGGAGGAACTTCTGCTCCAAAATCTAGTGACCGGAGACGCTGAAGCGCAGATTCGGGCTGCTACTCAACTCGGTAACCTAAGCAGCAAGCAAAGGCACAGTTTGGCTGACAGAGGTGTTATGCTCCCATTGATATCAATGCTTCATTCCCAAGACTTTGAAGCCATTGAAGCTGCTCTCTTAGCTCTGCTTAGTCTTGCCTTTGGTAGTGAAAGGAACAAGAGATGGATTTTGAAATCCGGGGCTTTACCGGCGGTGCTAAGCCTCCTTCAATGTGGAAGCAAAGCGTTGATGGAGCTCACAATAACAGCTTTGATGATTCTCTCATCTTGCAAGGCAAACAAGTTATCAATTGCCTCGTCTGGAGCCATTGAGCAAATCATTGAAATTCTCAATCGAGACTATGCTAATGCTAATGGCATGAACACAAACAAGGACACGATCACTATCAGCATGCAAGCCAGACTTGATGCCGTAGCTACACTCCACAACCTCTCAACTTGCCATCATATTATGCCATCTTTGGTCTCTTCTGGTGTAGTTTTCTCCTTGCTGCAAATAATCCGCAGCTGTGAAAAACCATCCCAACTGGTTGAGAAGGCTATTGCATTACTTGATGACATTGTTTCTTCATCCAAGATTCCGCTCAAGGAAACTGCAGGAACCTGTGGGGCAATTGGGGCATTAGTTGAGACCCTTGAAGAGGGATCAATGGAAAGCAAAGAGCACGCGGTGAGAATCCTGCTGTTCATATGCCAGAGCTGCAGAGAAAACTACGGAGGATTGATTTTGAGGGAAGGAGTAATGCCCGGGTTGCTGCAGTTGAAAGTAGATGGAACATGGAGGGCAAAAACTATGGCAGAGGAATTGCTGTTGCTTTTGAGGGACTGCTCGAGTTATGGTCCGAGTAATCAACAGTCCAGGAATGAGGTTATAGAGCAGATTATGCAAGAAATTGATGCAGAAGGAGAGGAAGTTGCTGGGAGTACAGCACTGAGGCTGCTTGCGAAGCTTAGCATCATGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm KAP

Protein Analysis

373

Amino Acids

40.44

Weight (kDa)

5.59

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 47 - 169 2.3e-07 ARMC5
ARM_PUB PF25598 155 - 328 1.4e-06 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016010)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14510
malus_domestica MD09G1024900.v1.1 MD17G1026200.v1.1
prunus_persica Prupe.3G292500_v2.0.a1
pyrus_communis pycom17g02130
rosa_chinensis RchiOBHm_Chr2g0173151
rosa_laevigata RLG00000022164
rosa_multiflora Rmu_sc0020537.1_g000001
rosa_roxburghii Rroxscaffold_2G00079180
rosa_rugosa Rorug02G0565700
rosa_samantha Rh2AG645900 Rh2BG656600 Rh2CG621700 Rh2DG670000
rosa_wichuraiana Rw2G053040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 736
AccII CGCG 1 cut(s) 821
AciI CCGC 4 cut(s) 344, 655, 734, 821
AclWI GGATC 2 cut(s) 12, 805
AcsI RAATTY 1 cut(s) 477
AcuI CTGAAG 1 cut(s) 153
AdeI CACNNNGTG 1 cut(s) 823
AfaI GTAC 2 cut(s) 11, 1078
AfiI CCNNNNNNNGG 1 cut(s) 793
AgsI TTSAA 8 cut(s) 24, 251, 260, 325, 362, 476, 791, 916
AjnI CCWGG 1 cut(s) 1010
AjuI GAANNNNNNNTTGG 2 cut(s) 57, 89
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 9 cut(s) 82, 263, 275, 388, 401, 569, 660, 852, 1102
AluI AGCT 9 cut(s) 82, 263, 275, 388, 401, 569, 660, 852, 1102
Alw21I GWGCWC 2 cut(s) 390, 819
Alw26I GTCTC 4 cut(s) 120, 483, 622, 776
AlwI GGATC 2 cut(s) 12, 805
Ama87I CYCGRG 2 cut(s) 899, 982
ApeKI GCWGC 8 cut(s) 149, 174, 263, 643, 657, 852, 907, 1090
ApoI RAATTY 1 cut(s) 477
AspLEI GCGC 1 cut(s) 139
AspS9I GGNCC 1 cut(s) 992
AsuC2I CCSGG 3 cut(s) 331, 900, 901
AsuHPI GGTGA 1 cut(s) 835
AvaI CYCGRG 2 cut(s) 899, 982
AvaII GGWCC 1 cut(s) 992
BanII GRGCYC 1 cut(s) 390
Bbv12I GWGCWC 2 cut(s) 390, 819
BbvI GCAGC 8 cut(s) 136, 186, 250, 630, 669, 839, 894, 1077
BccI CCATC 6 cut(s) 309, 376, 603, 616, 679, 917
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 1 cut(s) 1012
BcnI CCSGG 3 cut(s) 331, 900, 901
BcoDI GTCTC 4 cut(s) 120, 483, 622, 776
BfaI CTAG 1 cut(s) 116
BfmI CTRYAG 3 cut(s) 747, 853, 908
BisI GCNGC 8 cut(s) 150, 175, 264, 644, 658, 853, 908, 1091
BlpI GCTNAGC 2 cut(s) 350, 1103
BlsI GCNGC 8 cut(s) 151, 176, 265, 645, 659, 854, 909, 1092
Bme1390I CCNGG 4 cut(s) 331, 900, 901, 1012
Bme18I GGWCC 1 cut(s) 992
BmeT110I CYCGRG 2 cut(s) 899, 982
BmgT120I GGNCC 1 cut(s) 992
BmiI GGNNCC 2 cut(s) 457, 754
BmrFI CCNGG 4 cut(s) 331, 900, 901, 1012
BmsI GCATC 2 cut(s) 550, 1039
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 1 cut(s) 170
Bpu1102I GCTNAGC 2 cut(s) 350, 1103
BpuEI CTTGAG 1 cut(s) 722
BpuMI CCSGG 3 cut(s) 331, 900, 901
BsaBI GATNNNNATC 1 cut(s) 536
BsaI GGTCTC 2 cut(s) 622, 776
BsaJI CCNNGG 3 cut(s) 90, 330, 899
BsaWI WCCGGW 1 cut(s) 122
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 1050, 1080
Bsc4I CCNNNNNNNGG 1 cut(s) 793
Bse118I RCCGGY 1 cut(s) 340
Bse1I ACTGG 1 cut(s) 684
Bse8I GATNNNNATC 1 cut(s) 536
BseBI CCWGG 1 cut(s) 1012
BseDI CCNNGG 3 cut(s) 90, 330, 899
BseGI GGATG 2 cut(s) 671, 722
BseJI GATNNNNATC 1 cut(s) 536
BseLI CCNNNNNNNGG 1 cut(s) 793
BseMII CTCAG 1 cut(s) 1076
BseNI ACTGG 1 cut(s) 684
BseXI GCAGC 8 cut(s) 136, 186, 250, 630, 669, 839, 894, 1077
BseYI CCCAGC 1 cut(s) 1070
Bsh1236I CGCG 1 cut(s) 821
BsiHKAI GWGCWC 2 cut(s) 390, 819
BsiHKCI CYCGRG 2 cut(s) 899, 982
BsiSI CCGG 4 cut(s) 123, 330, 341, 900
BslFI GGGAC 1 cut(s) 989
BslI CCNNNNNNNGG 1 cut(s) 793
BsmAI GTCTC 4 cut(s) 120, 483, 622, 776
BsmBI CGTCTC 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 989
Bso31I GGTCTC 2 cut(s) 622, 776
BsoBI CYCGRG 2 cut(s) 899, 982
Bsp1286I GDGCHC 2 cut(s) 390, 819
Bsp143I GATC 3 cut(s) 4, 531, 797
Bsp1720I GCTNAGC 2 cut(s) 350, 1103
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 4 cut(s) 344, 655, 734, 821
BspCNI CTCAG 1 cut(s) 1077
BspFNI CGCG 1 cut(s) 821
BspLI GGNNCC 2 cut(s) 457, 754
BspMAI CTGCAG 3 cut(s) 751, 857, 912
BspPI GGATC 2 cut(s) 12, 805
BspTNI GGTCTC 2 cut(s) 622, 776
BsrBI CCGCTC 1 cut(s) 736
BsrFI RCCGGY 1 cut(s) 340
BsrI ACTGG 1 cut(s) 684
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 3 cut(s) 90, 330, 899
BssMI GATC 3 cut(s) 4, 531, 797
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 1012
Bst4CI ACNGT 2 cut(s) 191, 1008
Bst6I CTCTTC 3 cut(s) 25, 625, 786
BstAPI GCANNNNNTGC 1 cut(s) 1036
BstC8I GCNNGC 5 cut(s) 179, 545, 549, 819, 1095
BstDEI CTNAG 6 cut(s) 170, 271, 281, 350, 1085, 1103
BstDSI CCRYGG 1 cut(s) 90
BstEII GGTNACC 1 cut(s) 164
BstENI CCTNNNNNAGG 1 cut(s) 791
BstF5I GGATG 2 cut(s) 671, 722
BstFNI CGCG 1 cut(s) 821
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 3 cut(s) 7, 534, 800
BstMAI GTCTC 4 cut(s) 120, 483, 622, 776
BstMBI GATC 3 cut(s) 4, 531, 797
BstMWI GCNNNNNNNGC 8 cut(s) 260, 272, 341, 770, 852, 904, 1036, 1099
BstNI CCWGG 1 cut(s) 1012
BstNSI RCATGY 1 cut(s) 547
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 4 cut(s) 329, 898, 899, 1010
BstSFI CTRYAG 3 cut(s) 747, 853, 908
BstUI CGCG 1 cut(s) 821
BstV1I GCAGC 8 cut(s) 136, 186, 250, 630, 669, 839, 894, 1077
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 2 cut(s) 671, 722
BtsIMutI CAGTG 1 cut(s) 1082
Cac8I GCNNGC 5 cut(s) 179, 545, 549, 819, 1095
CfoI GCGC 1 cut(s) 139
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 1 cut(s) 992
Cfr9I CCCGGG 1 cut(s) 899
CseI GACGC 1 cut(s) 137
Csp6I GTAC 2 cut(s) 10, 1077
CviAII CATG 5 cut(s) 91, 511, 544, 930, 1111
CviQI GTAC 2 cut(s) 10, 1077
DdeI CTNAG 6 cut(s) 170, 271, 281, 350, 1085, 1103
DpnI GATC 3 cut(s) 6, 533, 799
DpnII GATC 3 cut(s) 4, 531, 797
DraIII CACNNNGTG 1 cut(s) 823
Eam1104I CTCTTC 3 cut(s) 25, 625, 786
EarI CTCTTC 3 cut(s) 25, 625, 786
Ecl136II GAGCTC 1 cut(s) 388
Eco130I CCWWGG 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 390
Eco31I GGTCTC 2 cut(s) 622, 776
Eco32I GATATC 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 992
Eco53kI GAGCTC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 153
Eco88I CYCGRG 2 cut(s) 899, 982
Eco91I GGTNACC 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 388
EcoNI CCTNNNNNAGG 1 cut(s) 791
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 1 cut(s) 1010
EcoRV GATATC 1 cut(s) 225
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 120
FaeI CATG 5 cut(s) 94, 514, 547, 933, 1114
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 1 cut(s) 989
FatI CATG 5 cut(s) 90, 510, 543, 929, 1110
FauNDI CATATG 1 cut(s) 842
Fnu4HI GCNGC 8 cut(s) 150, 175, 264, 644, 658, 853, 908, 1091
FokI GGATG 2 cut(s) 658, 709
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 8 cut(s) 150, 175, 264, 644, 658, 853, 908, 1091
FspBI CTAG 1 cut(s) 116
GlaI GCGC 1 cut(s) 138
GluI GCNGC 8 cut(s) 150, 175, 264, 644, 658, 853, 908, 1091
GsaI CCCAGC 1 cut(s) 1074
GsuI CTGGAG 1 cut(s) 474
HapII CCGG 4 cut(s) 123, 330, 341, 900
HgaI GACGC 1 cut(s) 137
HhaI GCGC 1 cut(s) 139
Hin1II CATG 5 cut(s) 94, 514, 547, 933, 1114
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HindIII AAGCTT 1 cut(s) 1100
HinfI GANTC 4 cut(s) 142, 409, 730, 828
HpaII CCGG 4 cut(s) 123, 330, 341, 900
HphI GGTGA 1 cut(s) 835
Hpy188I TCNGA 1 cut(s) 996
Hpy188III TCNNGA 2 cut(s) 453, 488
HpyAV CCTTC 5 cut(s) 96, 368, 682, 881, 1049
HpyCH4III ACNGT 2 cut(s) 191, 1008
HpyF10VI GCNNNNNNNGC 8 cut(s) 260, 272, 341, 770, 852, 904, 1036, 1099
HpyF3I CTNAG 6 cut(s) 170, 271, 281, 350, 1085, 1103
Hsp92II CATG 5 cut(s) 94, 514, 547, 933, 1114
HspAI GCGC 1 cut(s) 137
Kzo9I GATC 3 cut(s) 4, 531, 797
LmnI GCTCC 4 cut(s) 111, 219, 385, 455
Lsp1109I GCAGC 8 cut(s) 136, 186, 250, 630, 669, 839, 894, 1077
LweI GCATC 2 cut(s) 550, 1039
MaeI CTAG 1 cut(s) 116
MaeIII GTNAC 2 cut(s) 118, 164
MalI GATC 3 cut(s) 6, 533, 799
MbiI CCGCTC 1 cut(s) 736
MboI GATC 3 cut(s) 4, 531, 797
MboII GAAGA 4 cut(s) 12, 612, 711, 803
MfeI CAATTG 2 cut(s) 441, 765
MhlI GDGCHC 2 cut(s) 390, 819
MluCI AATT 5 cut(s) 441, 477, 765, 956, 1044
MspA1I CMGCKG 1 cut(s) 660
MspI CCGG 4 cut(s) 123, 330, 341, 900
MspR9I CCNGG 4 cut(s) 331, 900, 901, 1012
MunI CAATTG 2 cut(s) 441, 765
MvaI CCWGG 1 cut(s) 1012
MvnI CGCG 1 cut(s) 821
MwoI GCNNNNNNNGC 8 cut(s) 260, 272, 341, 770, 852, 904, 1036, 1099
NciI CCSGG 3 cut(s) 331, 900, 901
NcoI CCATGG 1 cut(s) 90
NdeI CATATG 1 cut(s) 842
NdeII GATC 3 cut(s) 4, 531, 797
NlaIII CATG 5 cut(s) 94, 514, 547, 933, 1114
NlaIV GGNNCC 2 cut(s) 457, 754
NmuCI GTSAC 1 cut(s) 118
NspI RCATGY 1 cut(s) 547
PaeI GCATGC 1 cut(s) 547
PaeR7I CTCGAG 1 cut(s) 982
PfeI GAWTC 4 cut(s) 142, 409, 730, 828
PfoI TCCNGGA 1 cut(s) 1010
PkrI GCNGC 8 cut(s) 151, 176, 265, 645, 659, 854, 909, 1092
Psp124BI GAGCTC 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 1010
PspEI GGTNACC 1 cut(s) 164
PspFI CCCAGC 1 cut(s) 1070
PspGI CCWGG 1 cut(s) 1010
PspN4I GGNNCC 2 cut(s) 457, 754
PspPI GGNCC 1 cut(s) 992
PspXI VCTCGAGB 1 cut(s) 982
PstI CTGCAG 3 cut(s) 751, 857, 912
PvuII CAGCTG 1 cut(s) 660
RsaI GTAC 2 cut(s) 11, 1078
RsaNI GTAC 2 cut(s) 10, 1077
SacI GAGCTC 1 cut(s) 390
SatI GCNGC 8 cut(s) 150, 175, 264, 644, 658, 853, 908, 1091
Sau3AI GATC 3 cut(s) 4, 531, 797
Sau96I GGNCC 1 cut(s) 992
ScrFI CCNGG 4 cut(s) 331, 900, 901, 1012
SduI GDGCHC 2 cut(s) 390, 819
SfaNI GCATC 2 cut(s) 550, 1039
SfcI CTRYAG 3 cut(s) 747, 853, 908
Sfr274I CTCGAG 1 cut(s) 982
SinI GGWCC 1 cut(s) 992
SlaI CTCGAG 1 cut(s) 982
SmaI CCCGGG 1 cut(s) 901
SmlI CTYRAG 2 cut(s) 737, 982
SmoI CTYRAG 2 cut(s) 737, 982
SphI GCATGC 1 cut(s) 547
Sse9I AATT 5 cut(s) 441, 477, 765, 956, 1044
SsiI CCGC 4 cut(s) 344, 655, 734, 821
SspMI CTAG 1 cut(s) 116
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 4 cut(s) 329, 898, 899, 1010
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 191, 1008
TaqI TCGA 2 cut(s) 487, 983
TasI AATT 5 cut(s) 441, 477, 765, 956, 1044
TatI WGTACW 2 cut(s) 9, 1076
TfiI GAWTC 4 cut(s) 142, 409, 730, 828
TscAI CASTG 1 cut(s) 1089
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 8 cut(s) 149, 174, 263, 643, 657, 852, 907, 1090
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 4 cut(s) 224, 527, 711, 829
TspGWI ACGGA 1 cut(s) 882
TspMI CCCGGG 1 cut(s) 899
TspRI CASTG 1 cut(s) 1089
VpaK11BI GGWCC 1 cut(s) 992
XagI CCTNNNNNAGG 1 cut(s) 791
XapI RAATTY 1 cut(s) 477
XceI RCATGY 1 cut(s) 547
XhoI CTCGAG 1 cut(s) 982
XmaI CCCGGG 1 cut(s) 899
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.