RLG00000022860

Cysteine-rich repeat secretory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008291
Physical Location & Seq
Forward (+)
13452456 .. 13454214
1759 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022860

Sequence Viewer

Length: 834 bp
ATGGTAGCTATAGTATCTCAAGAAAACGTGGTTTCTCGCACAGGAAGGCACTTGCAGCGCTACAGCAAAGACACCTTCGTCTACGGAGGCTGCACACAGGAGAAGTATGCGCCCAACTCGCCCTACGAGTCCAACATCAACTCGCTCCTGACCTCCCTGGTTAACTCAGCCACCTACTCCTCCTACAACAACTACACCGTCATGGGATCCAGCCCCCAAGACGTCATCTACGGCCTCTACCAATGCCGTGGCGACCTCTCCATGCCCGACTGCGCCACCTGTGTCGCCCGTGCAGTCAGCCAAGTTGGGAGCTTCTGCCCCTACAACTCCGGCGGTGCCCTTCAGCTGCAGGGCTGCTACATCAAGTACGACAACACCACCTTTCTTGGGGTGGAGGACAAGACGGTGGTGCTCAAAAAATGCGGGCCATCGGTCGGGTACGATTCGGACGGAATGAGCCGGAGGGATTCAGTGTTGGCTGCTTTGGTTGGCTCCGGTGGGCCGTACAGGGTGGGAGGGTCCGGGAACGTCCAAGGTTTGGCCCAATGCGTCGGGGATCTGAGCTTCACAGAGTGTCAGGATTGTGTGACGGAGGCAATCGGACGGGTGAAAAGTGAATGCGGTGGTGCTGTTTACGGTGATATGTTCTTGGCCAAGTGTTACGCCAGGTACTCAACTGGCAGTGCTCAATTTTACTCCAAGATGCATCATGATAAATCTAGCAATGAGGGTGAGAAGACATTTGCAATTATCATTGGATTATTAGCAGGAGTTGCTCTGCTCATCATCTTTCTTACTTTCATAAGGAAGGTGTTTGGGGGAAATGGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0009506 GO:0030054 GO:0055044
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stress-antifung

Protein Analysis

278

Amino Acids

29.68

Weight (kDa)

7.91

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 31 - 125 1.8e-21 Salt stress response/antifungal
Stress-antifung PF01657 173 - 226 3.3e-09 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
AatII GACGTC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 335
AccB7I CCANNNNNTGG 1 cut(s) 538
AccI GTMKAC 1 cut(s) 81
AciI CCGC 3 cut(s) 333, 423, 621
AclWI GGATC 3 cut(s) 201, 214, 564
AcoI YGGCCR 1 cut(s) 651
AcuI CTGAAG 1 cut(s) 326
AcyI GRCGYC 1 cut(s) 222
AdeI CACNNNGTG 1 cut(s) 573
AfaI GTAC 4 cut(s) 368, 440, 506, 671
AfeI AGCGCT 1 cut(s) 59
AfiI CCNNNNNNNGG 3 cut(s) 387, 434, 538
AjnI CCWGG 2 cut(s) 156, 665
AluBI AGCT 4 cut(s) 8, 312, 346, 564
AluI AGCT 4 cut(s) 8, 312, 346, 564
Alw21I GWGCWC 2 cut(s) 414, 688
AlwI GGATC 3 cut(s) 201, 214, 564
Aor51HI AGCGCT 1 cut(s) 59
AoxI GGCC 5 cut(s) 232, 425, 500, 540, 651
ApeKI GCWGC 5 cut(s) 55, 90, 346, 354, 479
AspLEI GCGC 3 cut(s) 60, 112, 275
AspS9I GGNCC 4 cut(s) 425, 500, 519, 541
AsuC2I CCSGG 1 cut(s) 523
AsuHPI GGTGA 3 cut(s) 619, 650, 743
AvaII GGWCC 1 cut(s) 519
BaeGI GKGCMC 1 cut(s) 340
BalI TGGCCA 1 cut(s) 653
BamHI GGATCC 1 cut(s) 206
BanI GGYRCC 1 cut(s) 335
BbsI GAAGAC 1 cut(s) 743
Bbv12I GWGCWC 2 cut(s) 414, 688
BbvI GCAGC 5 cut(s) 67, 77, 333, 341, 466
BccI CCATC 1 cut(s) 436
BceAI ACGGC 3 cut(s) 231, 247, 487
BciT130I CCWGG 2 cut(s) 158, 667
BcnI CCSGG 1 cut(s) 523
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 3 cut(s) 9, 61, 347
BfoI RGCGCY 1 cut(s) 61
BisI GCNGC 5 cut(s) 56, 91, 347, 355, 480
BlsI GCNGC 5 cut(s) 57, 92, 348, 356, 481
Bme1390I CCNGG 3 cut(s) 158, 523, 667
Bme18I GGWCC 1 cut(s) 519
BmgT120I GGNCC 4 cut(s) 425, 500, 519, 541
BmiI GGNNCC 4 cut(s) 208, 337, 493, 520
BmrFI CCNGG 3 cut(s) 158, 523, 667
BmsI GCATC 2 cut(s) 693, 715
BpiI GAAGAC 1 cut(s) 743
BpuMI CCSGG 1 cut(s) 523
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 156, 247, 532
BsaWI WCCGGW 1 cut(s) 494
Bsc4I CCNNNNNNNGG 3 cut(s) 387, 434, 538
Bse1I ACTGG 1 cut(s) 682
Bse3DI GCAATG 1 cut(s) 730
BseBI CCWGG 2 cut(s) 158, 667
BseDI CCNNGG 3 cut(s) 156, 247, 532
BseLI CCNNNNNNNGG 3 cut(s) 387, 434, 538
BseMI GCAATG 1 cut(s) 730
BseMII CTCAG 2 cut(s) 180, 551
BseNI ACTGG 1 cut(s) 682
BseRI GAGGAG 1 cut(s) 169
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 5 cut(s) 67, 77, 333, 341, 466
BsgI GTGCAG 2 cut(s) 76, 312
Bsh1285I CGRYCG 1 cut(s) 435
BshFI GGCC 5 cut(s) 234, 427, 502, 542, 653
BshNI GGYRCC 1 cut(s) 335
BsiEI CGRYCG 1 cut(s) 435
BsiHKAI GWGCWC 2 cut(s) 414, 688
BsiSI CCGG 4 cut(s) 330, 460, 495, 522
BslI CCNNNNNNNGG 3 cut(s) 387, 434, 538
BsmI GAATGC 1 cut(s) 623
BsnI GGCC 5 cut(s) 234, 427, 502, 542, 653
Bsp1286I GDGCHC 3 cut(s) 340, 414, 688
Bsp143I GATC 2 cut(s) 206, 556
BspACI CCGC 3 cut(s) 333, 423, 621
BspANI GGCC 5 cut(s) 234, 427, 502, 542, 653
BspCNI CTCAG 2 cut(s) 179, 552
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 4 cut(s) 208, 337, 493, 520
BspMAI CTGCAG 1 cut(s) 351
BspPI GGATC 3 cut(s) 201, 214, 564
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 730
BsrI ACTGG 1 cut(s) 682
BssECI CCNNGG 3 cut(s) 156, 247, 532
BssMI GATC 2 cut(s) 206, 556
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 532
Bst2UI CCWGG 2 cut(s) 158, 667
Bst4CI ACNGT 3 cut(s) 199, 406, 638
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 1 cut(s) 425
BstDEI CTNAG 2 cut(s) 166, 560
BstDSI CCRYGG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 3 cut(s) 60, 112, 275
BstKTI GATC 2 cut(s) 209, 559
BstMBI GATC 2 cut(s) 206, 556
BstMCI CGRYCG 1 cut(s) 435
BstMWI GCNNNNNNNGC 3 cut(s) 55, 118, 773
BstNI CCWGG 2 cut(s) 158, 667
BstSCI CCNGG 3 cut(s) 156, 521, 665
BstSFI CTRYAG 3 cut(s) 9, 61, 347
BstSLI GKGCMC 1 cut(s) 340
BstV1I GCAGC 5 cut(s) 67, 77, 333, 341, 466
BstV2I GAAGAC 1 cut(s) 743
BstX2I RGATCY 2 cut(s) 206, 556
BstXI CCANNNNNNTGG 1 cut(s) 248
BstYI RGATCY 2 cut(s) 206, 556
BsuRI GGCC 5 cut(s) 234, 427, 502, 542, 653
BtgI CCRYGG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 688
BtsIMutI CAGTG 2 cut(s) 477, 688
Cac8I GCNNGC 1 cut(s) 425
CciI TCATGA 1 cut(s) 709
CfoI GCGC 3 cut(s) 60, 112, 275
Cfr13I GGNCC 4 cut(s) 425, 500, 519, 541
CseI GACGC 1 cut(s) 538
Csp6I GTAC 4 cut(s) 367, 439, 505, 670
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviAII CATG 3 cut(s) 202, 262, 710
CviQI GTAC 4 cut(s) 367, 439, 505, 670
DdeI CTNAG 2 cut(s) 166, 560
DpnI GATC 2 cut(s) 208, 558
DpnII GATC 2 cut(s) 206, 556
DraIII CACNNNGTG 1 cut(s) 573
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 651
Eco130I CCWWGG 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 519
Eco47III AGCGCT 1 cut(s) 59
Eco57I CTGAAG 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 156, 665
EcoT14I CCWWGG 1 cut(s) 532
EcoT22I ATGCAT 1 cut(s) 708
ErhI CCWWGG 1 cut(s) 532
FaeI CATG 3 cut(s) 205, 265, 713
FaiI YATR 7 cut(s) 11, 108, 203, 263, 644, 711, 803
FatI CATG 3 cut(s) 201, 261, 709
FauI CCCGC 1 cut(s) 416
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 5 cut(s) 56, 91, 347, 355, 480
Fsp4HI GCNGC 5 cut(s) 56, 91, 347, 355, 480
FspBI CTAG 1 cut(s) 720
GlaI GCGC 3 cut(s) 59, 111, 274
GluI GCNGC 5 cut(s) 56, 91, 347, 355, 480
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 5 cut(s) 234, 427, 502, 542, 653
HapII CCGG 4 cut(s) 330, 460, 495, 522
HgaI GACGC 1 cut(s) 538
HhaI GCGC 3 cut(s) 60, 112, 275
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 3 cut(s) 205, 265, 713
Hin6I GCGC 3 cut(s) 58, 110, 273
HinP1I GCGC 3 cut(s) 58, 110, 273
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 3 cut(s) 128, 443, 467
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 4 cut(s) 330, 460, 495, 522
HphI GGTGA 3 cut(s) 619, 650, 743
Hpy166II GTNNAC 3 cut(s) 82, 163, 634
Hpy188I TCNGA 3 cut(s) 448, 561, 602
Hpy188III TCNNGA 4 cut(s) 20, 148, 578, 710
Hpy8I GTNNAC 3 cut(s) 82, 163, 634
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 4 cut(s) 39, 85, 350, 802
HpyCH4III ACNGT 3 cut(s) 199, 406, 638
HpyCH4IV ACGT 3 cut(s) 27, 222, 528
HpyCH4V TGCA 6 cut(s) 55, 93, 293, 349, 706, 746
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 118, 773
HpyF3I CTNAG 2 cut(s) 166, 560
HpySE526I ACGT 3 cut(s) 27, 222, 528
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 3 cut(s) 205, 265, 713
HspAI GCGC 3 cut(s) 58, 110, 273
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 2 cut(s) 206, 556
LmnI GCTCC 3 cut(s) 150, 309, 497
Lsp1109I GCAGC 5 cut(s) 67, 77, 333, 341, 466
LweI GCATC 2 cut(s) 693, 715
MaeI CTAG 1 cut(s) 720
MaeII ACGT 3 cut(s) 27, 222, 528
MaeIII GTNAC 2 cut(s) 586, 659
MalI GATC 2 cut(s) 208, 558
MboI GATC 2 cut(s) 206, 556
MboII GAAGA 1 cut(s) 748
MflI RGATCY 2 cut(s) 206, 556
MhlI GDGCHC 3 cut(s) 340, 414, 688
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 2 cut(s) 689, 747
MluNI TGGCCA 1 cut(s) 653
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 156
Mox20I TGGCCA 1 cut(s) 653
Mph1103I ATGCAT 1 cut(s) 708
MscI TGGCCA 1 cut(s) 653
MseI TTAA 1 cut(s) 162
MslI CAYNNNNRTG 1 cut(s) 200
Msp20I TGGCCA 1 cut(s) 653
MspA1I CMGCKG 1 cut(s) 346
MspI CCGG 4 cut(s) 330, 460, 495, 522
MspR9I CCNGG 3 cut(s) 158, 523, 667
Mva1269I GAATGC 1 cut(s) 623
MvaI CCWGG 2 cut(s) 158, 667
MwoI GCNNNNNNNGC 3 cut(s) 55, 118, 773
NciI CCSGG 1 cut(s) 523
NdeII GATC 2 cut(s) 206, 556
NlaIII CATG 3 cut(s) 205, 265, 713
NlaIV GGNNCC 4 cut(s) 208, 337, 493, 520
NmuCI GTSAC 1 cut(s) 586
NsiI ATGCAT 1 cut(s) 708
PagI TCATGA 1 cut(s) 709
PctI GAATGC 1 cut(s) 623
PfeI GAWTC 2 cut(s) 443, 467
PflMI CCANNNNNTGG 1 cut(s) 538
PfoI TCCNGGA 1 cut(s) 521
PkrI GCNGC 5 cut(s) 57, 92, 348, 356, 481
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
Psp6I CCWGG 2 cut(s) 156, 665
PspGI CCWGG 2 cut(s) 156, 665
PspN4I GGNNCC 4 cut(s) 208, 337, 493, 520
PspPI GGNCC 4 cut(s) 425, 500, 519, 541
PstI CTGCAG 1 cut(s) 351
PsuI RGATCY 2 cut(s) 206, 556
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 4 cut(s) 368, 440, 506, 671
RsaNI GTAC 4 cut(s) 367, 439, 505, 670
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 5 cut(s) 56, 91, 347, 355, 480
Sau3AI GATC 2 cut(s) 206, 556
Sau96I GGNCC 4 cut(s) 425, 500, 519, 541
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 3 cut(s) 158, 523, 667
SduI GDGCHC 3 cut(s) 340, 414, 688
SfaNI GCATC 2 cut(s) 693, 715
SfcI CTRYAG 3 cut(s) 9, 61, 347
SinI GGWCC 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 2 cut(s) 689, 747
SsiI CCGC 3 cut(s) 333, 423, 621
SspMI CTAG 1 cut(s) 720
StyD4I CCNGG 3 cut(s) 156, 521, 665
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 3 cut(s) 199, 406, 638
TaiI ACGT 3 cut(s) 30, 225, 531
TaqII GACCGA 1 cut(s) 421
TasI AATT 2 cut(s) 689, 747
TfiI GAWTC 2 cut(s) 443, 467
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 2 cut(s) 477, 688
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 5 cut(s) 55, 90, 346, 354, 479
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 1 cut(s) 790
TspGWI ACGGA 3 cut(s) 99, 465, 605
TspRI CASTG 2 cut(s) 477, 688
Van91I CCANNNNNTGG 1 cut(s) 538
VpaK11BI GGWCC 1 cut(s) 519
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 720
ZraI GACGTC 1 cut(s) 223
Zsp2I ATGCAT 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.