RLG00000023147

Hepatocellular carcinoma-associated antigen 59

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008705
Physical Location & Seq
Forward (+)
18848571 .. 18850383
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023147

Sequence Viewer

Length: 882 bp
ATGGGGAAGAATTTCAGAAAACGAAGCGCTCCCGCCGACGATAATGACGATCCTGAAAAGCTCAGCGACGGCGAAGAAGAAAGGAGATTGGCGCTGGAGGAGGTCAAGTTTCTTCAGAAGCAGAGGCAGAGGAAGTCTGGGATCCCTGCCCTAACCACTGGTTTTGACGCACACCCTCTTCCTAATGCTAAGGACAAGAACAACGACTCCGGTAAGAGCAAGAACAAGAAAGCTAGTGGCGGCCACTCTCCTGGCAATAATAACGCTGAAGGCGGCGGCGGCGGCGATGGGGATAATGATGATTGGGCTCTTACTCGGACCTTTGCTGAGGAAACTGAAGAAGCCGACGAAGATCCGAATATGTTGAGTTTTATTGAACGAGAGATAGCAAAGAAGAAGGGCAAGAAAGTTGAAGCACCGGATGAAGTCGAGAATGAATTGCAGCGTGCTGAGGATGAGTTGTACAAGATACCTGACCATCTGAAAGTGAAAAAGCGGAACTCGGAAGAGAGCTCGACTCAGTGGACTACTGGGATTGCCGAGGTTCAGCTCCCCATCGAATATAAGTTGAGAAATATCGAGGAGACTGAGGCTGCAAAGAAGTTTTTGCATGAAAAGCGGCTTGTAGGACAGACAAAATCAGAATTTAGCATCCCCTCAAGTTACAGTGCTGATTATTTCCAGCGTGGCAAGGATTATGCTGAAAAACTTAGAAGAGAACATCCTGAGCTCTACAAAGACAAAAGTTCACAGGATAATGGTGGTGTTCCCAACCAAAATGATACTGGCACAGATGCAGCAGGACAAAGGCAAGCTGCAACAGATGAGTTCATGCTTGAGCGCTTCCGCAAAAGAGAGCGTCACCGTGGAATGCGGAGATGA

Protein Analysis

294

Amino Acids

33.24

Weight (kDa)

5.86

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hep_59 PF07052 107 - 203 3.2e-26 Hepatocellular carcinoma-associated antigen 59
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 44, 136, 149, 347
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 2 cut(s) 10, 644
AcuI CTGAAG 3 cut(s) 98, 288, 357
AfaI GTAC 1 cut(s) 464
AfeI AGCGCT 2 cut(s) 28, 842
AgsI TTSAA 2 cut(s) 377, 413
AjnI CCWGG 1 cut(s) 250
AloI GAACNNNNNNTCC 2 cut(s) 191, 223
AluBI AGCT 6 cut(s) 61, 233, 513, 550, 730, 815
AluI AGCT 6 cut(s) 61, 233, 513, 550, 730, 815
Alw21I GWGCWC 2 cut(s) 515, 732
Alw26I GTCTC 1 cut(s) 578
AlwI GGATC 4 cut(s) 44, 136, 149, 347
Aor51HI AGCGCT 2 cut(s) 28, 842
AoxI GGCC 1 cut(s) 241
ApeKI GCWGC 4 cut(s) 442, 593, 797, 815
ApoI RAATTY 2 cut(s) 10, 644
Asp700I GAANNNNTTC 1 cut(s) 11
AspLEI GCGC 3 cut(s) 29, 94, 843
AspS9I GGNCC 1 cut(s) 318
AsuHPI GGTGA 1 cut(s) 854
AvaII GGWCC 1 cut(s) 318
BamHI GGATCC 1 cut(s) 141
BanII GRGCYC 3 cut(s) 310, 515, 732
Bbv12I GWGCWC 2 cut(s) 515, 732
BbvCI CCTCAGC 2 cut(s) 327, 450
BbvI GCAGC 4 cut(s) 454, 580, 802, 809
BccI CCATC 3 cut(s) 281, 486, 563
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 578
BfaI CTAG 1 cut(s) 234
BfoI RGCGCY 3 cut(s) 30, 95, 844
BlpI GCTNAGC 1 cut(s) 62
Bme1390I CCNGG 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 318
BmgT120I GGNCC 1 cut(s) 318
BmiI GGNNCC 1 cut(s) 143
BmrFI CCNGG 1 cut(s) 252
BmrI ACTGGG 1 cut(s) 540
BmsI GCATC 2 cut(s) 660, 784
BmuI ACTGGG 1 cut(s) 540
BplI GAGNNNNNCTC 2 cut(s) 502, 534
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 4 cut(s) 189, 327, 450, 726
Bpu1102I GCTNAGC 1 cut(s) 62
BpuEI CTTGAG 2 cut(s) 643, 857
BsaJI CCNNGG 2 cut(s) 540, 865
BsaWI WCCGGW 2 cut(s) 209, 418
BsaXI ACNNNNNCTCC 4 cut(s) 92, 122, 191, 221
Bse1I ACTGG 3 cut(s) 163, 535, 790
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 540, 865
BseGI GGATG 4 cut(s) 427, 460, 651, 721
BseMII CTCAG 6 cut(s) 76, 318, 441, 533, 579, 717
BseNI ACTGG 3 cut(s) 163, 535, 790
BseRI GAGGAG 2 cut(s) 113, 596
BseXI GCAGC 4 cut(s) 454, 580, 802, 809
BshFI GGCC 1 cut(s) 243
BsiHKAI GWGCWC 2 cut(s) 515, 732
BsiSI CCGG 2 cut(s) 210, 419
BsmAI GTCTC 1 cut(s) 578
BsmI GAATGC 1 cut(s) 876
BsnI GGCC 1 cut(s) 243
Bsp1286I GDGCHC 3 cut(s) 310, 515, 732
Bsp1407I TGTACA 1 cut(s) 462
Bsp143I GATC 3 cut(s) 49, 141, 352
Bsp1720I GCTNAGC 1 cut(s) 62
BspANI GGCC 1 cut(s) 243
BspCNI CTCAG 6 cut(s) 75, 319, 442, 532, 580, 718
BspLI GGNNCC 1 cut(s) 143
BspPI GGATC 4 cut(s) 44, 136, 149, 347
BsrGI TGTACA 1 cut(s) 462
BsrI ACTGG 3 cut(s) 163, 535, 790
BssECI CCNNGG 2 cut(s) 540, 865
BssMI GATC 3 cut(s) 49, 141, 352
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 2 cut(s) 668, 866
Bst6I CTCTTC 3 cut(s) 183, 501, 709
BstAUI TGTACA 1 cut(s) 462
BstC8I GCNNGC 2 cut(s) 447, 813
BstDEI CTNAG 8 cut(s) 62, 189, 327, 450, 519, 588, 710, 726
BstDSI CCRYGG 1 cut(s) 865
BstF5I GGATG 4 cut(s) 427, 460, 651, 721
BstH2I RGCGCY 3 cut(s) 30, 95, 844
BstHHI GCGC 3 cut(s) 29, 94, 843
BstKTI GATC 3 cut(s) 52, 144, 355
BstMAI GTCTC 1 cut(s) 578
BstMBI GATC 3 cut(s) 49, 141, 352
BstMWI GCNNNNNNNGC 3 cut(s) 279, 282, 616
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstV1I GCAGC 4 cut(s) 454, 580, 802, 809
BstX2I RGATCY 2 cut(s) 141, 352
BstXI CCANNNNNNTGG 1 cut(s) 251
BstYI RGATCY 2 cut(s) 141, 352
BsuRI GGCC 1 cut(s) 243
BtgI CCRYGG 1 cut(s) 865
BtgZI GCGATG 1 cut(s) 300
BtsCI GGATG 4 cut(s) 427, 460, 651, 721
BtsIMutI CAGTG 3 cut(s) 156, 527, 673
Cac8I GCNNGC 2 cut(s) 447, 813
CfoI GCGC 3 cut(s) 29, 94, 843
Cfr13I GGNCC 1 cut(s) 318
CseI GACGC 2 cut(s) 176, 848
Csp6I GTAC 1 cut(s) 463
CspCI CAANNNNNGTGG 2 cut(s) 145, 180
CviAII CATG 2 cut(s) 611, 832
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 8 cut(s) 62, 189, 327, 450, 519, 588, 710, 726
DpnI GATC 3 cut(s) 51, 143, 354
DpnII GATC 3 cut(s) 49, 141, 352
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 3 cut(s) 183, 501, 709
EarI CTCTTC 3 cut(s) 183, 501, 709
Ecl136II GAGCTC 2 cut(s) 513, 730
Eco24I GRGCYC 3 cut(s) 310, 515, 732
Eco47I GGWCC 1 cut(s) 318
Eco47III AGCGCT 2 cut(s) 28, 842
Eco53kI GAGCTC 2 cut(s) 513, 730
Eco57I CTGAAG 3 cut(s) 98, 288, 357
EcoICRI GAGCTC 2 cut(s) 513, 730
EcoRII CCWGG 1 cut(s) 250
EcoT38I GRGCYC 3 cut(s) 310, 515, 732
FaeI CATG 2 cut(s) 614, 835
FaiI YATR 5 cut(s) 362, 564, 612, 699, 833
FatI CATG 2 cut(s) 610, 831
FauI CCCGC 1 cut(s) 40
FokI GGATG 4 cut(s) 434, 467, 638, 708
FriOI GRGCYC 3 cut(s) 310, 515, 732
FspBI CTAG 1 cut(s) 234
GlaI GCGC 3 cut(s) 28, 93, 842
GsuI CTGGAG 1 cut(s) 116
HaeII RGCGCY 3 cut(s) 30, 95, 844
HaeIII GGCC 1 cut(s) 243
HapII CCGG 2 cut(s) 210, 419
HgaI GACGC 2 cut(s) 176, 848
HhaI GCGC 3 cut(s) 29, 94, 843
Hin1II CATG 2 cut(s) 614, 835
Hin6I GCGC 3 cut(s) 27, 92, 841
HinP1I GCGC 3 cut(s) 27, 92, 841
HinfI GANTC 2 cut(s) 206, 517
HpaII CCGG 2 cut(s) 210, 419
HphI GGTGA 1 cut(s) 854
Hpy166II GTNNAC 2 cut(s) 525, 749
Hpy188I TCNGA 7 cut(s) 17, 117, 318, 357, 483, 505, 643
Hpy188III TCNNGA 3 cut(s) 53, 430, 725
Hpy8I GTNNAC 2 cut(s) 525, 749
Hpy99I CGWCG 3 cut(s) 41, 71, 350
HpyAV CCTTC 2 cut(s) 263, 391
HpyCH4III ACNGT 2 cut(s) 668, 866
HpyCH4V TGCA 5 cut(s) 442, 596, 610, 797, 818
HpyF10VI GCNNNNNNNGC 3 cut(s) 279, 282, 616
HpyF3I CTNAG 8 cut(s) 62, 189, 327, 450, 519, 588, 710, 726
Hsp92II CATG 2 cut(s) 614, 835
HspAI GCGC 3 cut(s) 27, 92, 841
Kzo9I GATC 3 cut(s) 49, 141, 352
LmnI GCTCC 2 cut(s) 34, 555
Lsp1109I GCAGC 4 cut(s) 454, 580, 802, 809
LweI GCATC 2 cut(s) 660, 784
MaeI CTAG 1 cut(s) 234
MaeIII GTNAC 2 cut(s) 662, 860
MalI GATC 3 cut(s) 51, 143, 354
MboI GATC 3 cut(s) 49, 141, 352
MflI RGATCY 2 cut(s) 141, 352
MhlI GDGCHC 3 cut(s) 310, 515, 732
MluCI AATT 3 cut(s) 10, 437, 644
MlyI GAGTC 2 cut(s) 200, 511
MroXI GAANNNNTTC 1 cut(s) 11
MspI CCGG 2 cut(s) 210, 419
MspR9I CCNGG 1 cut(s) 252
Mva1269I GAATGC 1 cut(s) 876
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 3 cut(s) 279, 282, 616
NdeII GATC 3 cut(s) 49, 141, 352
NlaIII CATG 2 cut(s) 614, 835
NlaIV GGNNCC 1 cut(s) 143
NmeAIII GCCGAG 1 cut(s) 565
NmuCI GTSAC 1 cut(s) 860
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 1 cut(s) 876
PdmI GAANNNNTTC 1 cut(s) 11
PleI GAGTC 2 cut(s) 200, 511
PpsI GAGTC 2 cut(s) 200, 511
Psp124BI GAGCTC 2 cut(s) 515, 732
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 1 cut(s) 318
PsuI RGATCY 2 cut(s) 141, 352
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SacI GAGCTC 2 cut(s) 515, 732
Sau3AI GATC 3 cut(s) 49, 141, 352
Sau96I GGNCC 1 cut(s) 318
SchI GAGTC 2 cut(s) 200, 511
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 3 cut(s) 310, 515, 732
SfaNI GCATC 2 cut(s) 660, 784
SinI GGWCC 1 cut(s) 318
SmlI CTYRAG 2 cut(s) 658, 836
SmoI CTYRAG 2 cut(s) 658, 836
Sse9I AATT 3 cut(s) 10, 437, 644
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 2 cut(s) 515, 732
StyD4I CCNGG 1 cut(s) 250
TaaI ACNGT 2 cut(s) 668, 866
TaqI TCGA 4 cut(s) 429, 515, 558, 579
TasI AATT 3 cut(s) 10, 437, 644
TatI WGTACW 1 cut(s) 462
TauI GCSGC 6 cut(s) 243, 276, 279, 282, 285, 622
TscAI CASTG 3 cut(s) 163, 527, 673
TseFI GTSAC 1 cut(s) 860
TseI GCWGC 4 cut(s) 442, 593, 797, 815
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 4 cut(s) 438, 450, 627, 820
TspRI CASTG 3 cut(s) 163, 527, 673
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 2 cut(s) 10, 644
XcmI CCANNNNNNNNNTGG 1 cut(s) 782
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.