RLG00000024772

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016364
Physical Location & Seq
Forward (+)
38998076 .. 39000192
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024772

Sequence Viewer

Length: 1650 bp
ATGAGAAAGCAAAGCATTCACGAAATAGTTTGTTACAGATATGACCATTCTTTTCCTCTTGCACTGGAGGCGGTTCAGAATTGGTCAAATGTATGTAATAAATTTTGTATACGTACAGATCAGAAGTGGAGCCAGAATACAACTAATTTGGAGTCACTTGTTCCAGGGTGTTCCTATTCTCATATATCCATCTTCATAGTTGGCTACAAATCAAAGCCGATATGCAGTGATTGGGGAAAGAGCTCAAATTGGTGGTCCGACGACAAAGGAAAACCTGCCGAGATGACTGAATTCCAGCAGGAAATCCAGCACGTAGAAGCAATTGACAAGAAGAAAGAGTGGCATCGGGAGATTACTGAAAAGCTTCACCGCAAGGTGGAACGTGGTTCTGGATGTCTGAGCATCCACACGGTTCCAACGAGACTTCGACGAGTGAAGGATGAGGCCTACAACTGTCGCGTTGTCTCAATTGGTCCTCTGCATCTAGGCAACCACGACCTTGCAGCCATGAGAGAGCGTAAATGGGCTTACACGCATCACTTATTTCAGCGAATTGAAGATCGTCAGCAGTTTCTAGAGTGTTGGAAAGAGTGCACGAGTGCTGTTTATGATTTAGACTCAGAAATTCGTGGATGCTACGCTAAAAGGATGAGCCACAAAGACAAGGGACACAAGGAGCTTGCAGAAGTTGTATTAATTGACGGATGCTTCATTCTGGAACTTTTCCTCAAGTATGAGGAACACTTGAATATGACACTGGATTATCAATCTGATGCAATATTCAATAGTGCTTGGATGATCGCGGCTCTTCAGCATGATCTAGCACTGCTCGAAAACCAGATCCCCTTTATGATTCTCGAACGTTTATATGAGGCCATTAGACCTCATCTAAAGGATAGTCGACCAAACTCTGTTGCGAGCCTTGCTCTTAATTTTTTTCAACCTATGAACTATAAGGCATTTTTAGAAGATTGGGATACGCAGTACAACCATTTGCTTGATGTCTTGCACAAATTTTACCTCCCAAGTAATTGTGACATCTCAGTAGAAATTGAAATGATTACCGAAATGAAACATCATTCCAGACCTGAACCAGTATTCGGCAGTACTAAAAAATGGGGATTCAATTTTTGTGCTTCAGAGCTTTCGGAGGCTGGAATCGAGTTTCAGAAGCAGCAGAGGACGGACCATTTGTTGAACATAACTTTCAGGAGGGGAGTAATTAAAATTCCACCGATTTTCATTGACGATTCACTCTTGAGGAACTTGATTGCTTTCGAGCAATGCAGTCTTGGCAGCCTGCATCACATGACATCATACGCAATTCTCTTGAAGAGTCTCATTCGTTACCCGCGAGATATCAGACTACTTAAGCAAAGAGGGATCATAAGTTCCAATTGGATTAGAGAAGAAGAGTACTTGTCTCAACTCAAAAGTATACTTGATGAAGTTGTTGTGAAGGAGTTCTACTTTGGTGATTTGTGTGACAAAGTGAATGCTTACCATGGCAAGTTCTGGTTTCGCAGAGGTATAAAGTACATTTATCGCACTTATTTCTCCACAGCATGGAGTATGATATCATTCGTTGCAGCCATTATACTTTTCATTCTCACAGCTTTACAAACATTTTTTACTGTCCGCCCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

550

Amino Acids

64.64

Weight (kDa)

8.08

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 135 - 537 2.8e-96 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 283
AccB7I CCANNNNNTGG 1 cut(s) 1566
AccI GTMKAC 3 cut(s) 109, 901, 1438
AccII CGCG 3 cut(s) 459, 803, 1354
AciI CCGC 5 cut(s) 71, 370, 803, 1352, 1639
AclI AACGTT 1 cut(s) 862
AclWI GGATC 2 cut(s) 835, 1391
AcsI RAATTY 5 cut(s) 101, 290, 624, 1013, 1227
AcuI CTGAAG 2 cut(s) 794, 1122
AfaI GTAC 5 cut(s) 115, 986, 1108, 1418, 1538
AfiI CCNNNNNNNGG 3 cut(s) 376, 1100, 1566
AflII CTTAAG 1 cut(s) 1370
AgsI TTSAA 8 cut(s) 557, 748, 784, 941, 1055, 1126, 1198, 1333
AjnI CCWGG 1 cut(s) 163
AloI GAACNNNNNNTCC 2 cut(s) 1375, 1407
AluBI AGCT 5 cut(s) 243, 364, 679, 1144, 1616
AluI AGCT 5 cut(s) 243, 364, 679, 1144, 1616
Alw21I GWGCWC 2 cut(s) 245, 596
Alw26I GTCTC 4 cut(s) 415, 469, 1343, 1428
Alw44I GTGCAC 1 cut(s) 592
AlwI GGATC 2 cut(s) 835, 1391
AoxI GGCC 2 cut(s) 444, 873
ApaLI GTGCAC 1 cut(s) 592
ApeKI GCWGC 4 cut(s) 503, 1174, 1296, 1589
ApoI RAATTY 5 cut(s) 101, 290, 624, 1013, 1227
ArsI GACNNNNNNTTYG 4 cut(s) 239, 271, 1174, 1206
AseI ATTAAT 1 cut(s) 695
Asp700I GAANNNNTTC 2 cut(s) 363, 1463
AspS9I GGNCC 3 cut(s) 255, 473, 1186
AsuHPI GGTGA 2 cut(s) 359, 1487
AvaII GGWCC 3 cut(s) 255, 473, 1186
BaeGI GKGCMC 1 cut(s) 596
BanII GRGCYC 1 cut(s) 245
BarI GAAGNNNNNNTAC 2 cut(s) 1451, 1483
BauI CACGAG 1 cut(s) 595
Bbv12I GWGCWC 2 cut(s) 245, 596
BbvI GCAGC 4 cut(s) 515, 1186, 1308, 1601
BccI CCATC 1 cut(s) 197
BciT130I CCWGG 1 cut(s) 165
BciVI GTATCC 1 cut(s) 970
BcoDI GTCTC 4 cut(s) 415, 469, 1343, 1428
BfaI CTAG 3 cut(s) 485, 575, 821
BfrI CTTAAG 1 cut(s) 1370
BfuAI ACCTGC 1 cut(s) 283
BfuI GTATCC 1 cut(s) 970
BisI GCNGC 5 cut(s) 504, 804, 1175, 1297, 1590
BlsI GCNGC 5 cut(s) 505, 805, 1176, 1298, 1591
BmcAI AGTACT 2 cut(s) 1108, 1418
Bme1390I CCNGG 1 cut(s) 165
Bme18I GGWCC 3 cut(s) 255, 473, 1186
BmgT120I GGNCC 3 cut(s) 255, 473, 1186
BmiI GGNNCC 2 cut(s) 131, 414
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 8 cut(s) 352, 411, 490, 544, 623, 695, 763, 1312
BplI GAGNNNNNCTC 2 cut(s) 910, 942
BpmI CTGGAG 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 713, 1279
BsaAI YACGTR 2 cut(s) 113, 313
BsaJI CCNNGG 2 cut(s) 164, 1504
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 1100, 1566
Bse1I ACTGG 3 cut(s) 69, 762, 1094
Bse3DI GCAATG 1 cut(s) 1289
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 164, 1504
BseGI GGATG 7 cut(s) 398, 402, 445, 638, 654, 710, 801
BseLI CCNNNNNNNGG 3 cut(s) 376, 1100, 1566
BseMI GCAATG 1 cut(s) 1289
BseMII CTCAG 3 cut(s) 389, 633, 1056
BseNI ACTGG 3 cut(s) 69, 762, 1094
BseSI GKGCMC 1 cut(s) 596
BseXI GCAGC 4 cut(s) 515, 1186, 1308, 1601
Bsh1236I CGCG 3 cut(s) 459, 803, 1354
BshFI GGCC 2 cut(s) 446, 875
BsiHKAI GWGCWC 2 cut(s) 245, 596
BslFI GGGAC 1 cut(s) 681
BslI CCNNNNNNNGG 3 cut(s) 376, 1100, 1566
BsmAI GTCTC 4 cut(s) 415, 469, 1343, 1428
BsmFI GGGAC 1 cut(s) 681
BsmI GAATGC 2 cut(s) 15, 1501
BsnI GGCC 2 cut(s) 446, 875
Bsp1286I GDGCHC 2 cut(s) 245, 596
Bsp143I GATC 6 cut(s) 118, 559, 798, 817, 840, 1383
Bsp19I CCATGG 1 cut(s) 1504
BspACI CCGC 5 cut(s) 71, 370, 803, 1352, 1639
BspANI GGCC 2 cut(s) 446, 875
BspCNI CTCAG 3 cut(s) 390, 632, 1055
BspFNI CGCG 3 cut(s) 459, 803, 1354
BspLI GGNNCC 2 cut(s) 131, 414
BspMI ACCTGC 1 cut(s) 283
BspPI GGATC 2 cut(s) 835, 1391
BspQI GCTCTTC 1 cut(s) 813
BspTI CTTAAG 1 cut(s) 1370
BsrDI GCAATG 1 cut(s) 1289
BsrI ACTGG 3 cut(s) 69, 762, 1094
BssECI CCNNGG 2 cut(s) 164, 1504
BssMI GATC 6 cut(s) 118, 559, 798, 817, 840, 1383
BssNAI GTATAC 2 cut(s) 110, 1439
BssSI CACGAG 1 cut(s) 595
BssT1I CCWWGG 1 cut(s) 1504
Bst1107I GTATAC 2 cut(s) 110, 1439
Bst2BI CACGAG 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 3 cut(s) 412, 455, 1636
Bst6I CTCTTC 3 cut(s) 813, 1328, 1407
BstAFI CTTAAG 1 cut(s) 1370
BstBAI YACGTR 2 cut(s) 113, 313
BstC8I GCNNGC 3 cut(s) 681, 919, 1301
BstDEI CTNAG 3 cut(s) 398, 619, 1042
BstDSI CCRYGG 1 cut(s) 1504
BstF5I GGATG 7 cut(s) 398, 402, 445, 638, 654, 710, 801
BstFNI CGCG 3 cut(s) 459, 803, 1354
BstKTI GATC 6 cut(s) 121, 562, 801, 820, 843, 1386
BstMAI GTCTC 4 cut(s) 415, 469, 1343, 1428
BstMBI GATC 6 cut(s) 118, 559, 798, 817, 840, 1383
BstMWI GCNNNNNNNGC 3 cut(s) 68, 923, 1293
BstNI CCWGG 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 163
BstSLI GKGCMC 1 cut(s) 596
BstSNI TACGTA 1 cut(s) 113
BstUI CGCG 3 cut(s) 459, 803, 1354
BstV1I GCAGC 4 cut(s) 515, 1186, 1308, 1601
BstX2I RGATCY 1 cut(s) 840
BstYI RGATCY 1 cut(s) 840
BstZ17I GTATAC 2 cut(s) 110, 1439
BsuI GTATCC 1 cut(s) 970
BsuRI GGCC 2 cut(s) 446, 875
BtgI CCRYGG 1 cut(s) 1504
BtsCI GGATG 7 cut(s) 398, 402, 445, 638, 654, 710, 801
BtsI GCAGTG 2 cut(s) 232, 824
BtsIMutI CAGTG 4 cut(s) 62, 232, 755, 824
BveI ACCTGC 1 cut(s) 283
Cac8I GCNNGC 3 cut(s) 681, 919, 1301
Cfr13I GGNCC 3 cut(s) 255, 473, 1186
Csp6I GTAC 5 cut(s) 114, 985, 1107, 1417, 1537
CviAII CATG 5 cut(s) 508, 815, 1309, 1505, 1566
CviQI GTAC 5 cut(s) 114, 985, 1107, 1417, 1537
DdeI CTNAG 3 cut(s) 398, 619, 1042
DpnI GATC 6 cut(s) 120, 561, 800, 819, 842, 1385
DpnII GATC 6 cut(s) 118, 559, 798, 817, 840, 1383
Eam1104I CTCTTC 3 cut(s) 813, 1328, 1407
EarI CTCTTC 3 cut(s) 813, 1328, 1407
EciI GGCGGA 1 cut(s) 1628
Ecl136II GAGCTC 1 cut(s) 243
Eco105I TACGTA 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 1504
Eco147I AGGCCT 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 245
Eco32I GATATC 2 cut(s) 1360, 1578
Eco47I GGWCC 3 cut(s) 255, 473, 1186
Eco53kI GAGCTC 1 cut(s) 243
Eco57I CTGAAG 2 cut(s) 794, 1122
EcoICRI GAGCTC 1 cut(s) 243
EcoRI GAATTC 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 163
EcoRV GATATC 2 cut(s) 1360, 1578
EcoT14I CCWWGG 1 cut(s) 1504
EcoT38I GRGCYC 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 1504
FaeI CATG 5 cut(s) 511, 818, 1312, 1508, 1569
FaqI GGGAC 1 cut(s) 681
FatI CATG 5 cut(s) 507, 814, 1308, 1504, 1565
FauI CCCGC 1 cut(s) 1359
FblI GTMKAC 3 cut(s) 109, 901, 1438
Fnu4HI GCNGC 5 cut(s) 504, 804, 1175, 1297, 1590
FokI GGATG 7 cut(s) 389, 405, 452, 645, 661, 717, 808
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 5 cut(s) 504, 804, 1175, 1297, 1590
FspBI CTAG 3 cut(s) 485, 575, 821
GluI GCNGC 5 cut(s) 504, 804, 1175, 1297, 1590
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 2 cut(s) 446, 875
Hin1II CATG 5 cut(s) 511, 818, 1312, 1508, 1569
HincII GTYRAC 1 cut(s) 902
HindII GTYRAC 1 cut(s) 902
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 7 cut(s) 152, 617, 853, 1122, 1158, 1250, 1336
HphI GGTGA 2 cut(s) 359, 1487
Hpy166II GTNNAC 4 cut(s) 110, 594, 902, 1439
Hpy8I GTNNAC 4 cut(s) 110, 594, 902, 1439
Hpy99I CGWCG 2 cut(s) 263, 432
HpyAV CCTTC 2 cut(s) 430, 1453
HpyCH4III ACNGT 3 cut(s) 412, 455, 1636
HpyCH4IV ACGT 4 cut(s) 112, 312, 382, 862
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 923, 1293
HpyF3I CTNAG 3 cut(s) 398, 619, 1042
HpySE526I ACGT 4 cut(s) 112, 312, 382, 862
Hsp92II CATG 5 cut(s) 511, 818, 1312, 1508, 1569
Kzo9I GATC 6 cut(s) 118, 559, 798, 817, 840, 1383
LguI GCTCTTC 1 cut(s) 813
LmnI GCTCC 2 cut(s) 129, 676
Lsp1109I GCAGC 4 cut(s) 515, 1186, 1308, 1601
LweI GCATC 8 cut(s) 352, 411, 490, 544, 623, 695, 763, 1312
MaeI CTAG 3 cut(s) 485, 575, 821
MaeII ACGT 4 cut(s) 112, 312, 382, 862
MaeIII GTNAC 5 cut(s) 32, 153, 1034, 1346, 1484
MalI GATC 6 cut(s) 120, 561, 800, 819, 842, 1385
MboI GATC 6 cut(s) 118, 559, 798, 817, 840, 1383
MboII GAAGA 8 cut(s) 184, 343, 569, 800, 980, 1345, 1421, 1424
MfeI CAATTG 3 cut(s) 321, 468, 1396
MflI RGATCY 1 cut(s) 840
MhlI GDGCHC 2 cut(s) 245, 596
MlyI GAGTC 3 cut(s) 161, 611, 1345
MmeI TCCRAC 3 cut(s) 282, 440, 563
MroXI GAANNNNTTC 2 cut(s) 363, 1463
MseI TTAA 4 cut(s) 695, 930, 1224, 1371
MspCI CTTAAG 1 cut(s) 1370
MspR9I CCNGG 1 cut(s) 165
MunI CAATTG 3 cut(s) 321, 468, 1396
Mva1269I GAATGC 2 cut(s) 15, 1501
MvaI CCWGG 1 cut(s) 165
MvnI CGCG 3 cut(s) 459, 803, 1354
MwoI GCNNNNNNNGC 3 cut(s) 68, 923, 1293
NcoI CCATGG 1 cut(s) 1504
NdeII GATC 6 cut(s) 118, 559, 798, 817, 840, 1383
NlaIII CATG 5 cut(s) 511, 818, 1312, 1508, 1569
NlaIV GGNNCC 2 cut(s) 131, 414
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 3 cut(s) 153, 1034, 1484
PceI AGGCCT 1 cut(s) 446
PciSI GCTCTTC 1 cut(s) 813
PcsI WCGNNNNNNNCGW 2 cut(s) 416, 1351
PctI GAATGC 2 cut(s) 15, 1501
PdmI GAANNNNTTC 2 cut(s) 363, 1463
PfeI GAWTC 4 cut(s) 853, 1122, 1158, 1250
PflMI CCANNNNNTGG 1 cut(s) 1566
PkrI GCNGC 5 cut(s) 505, 805, 1176, 1298, 1591
PleI GAGTC 3 cut(s) 160, 611, 1344
PpsI GAGTC 3 cut(s) 160, 611, 1344
Ppu21I YACGTR 2 cut(s) 113, 313
PshBI ATTAAT 1 cut(s) 695
Psp124BI GAGCTC 1 cut(s) 245
Psp1406I AACGTT 1 cut(s) 862
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 2 cut(s) 131, 414
PspPI GGNCC 3 cut(s) 255, 473, 1186
PsuI RGATCY 1 cut(s) 840
RsaI GTAC 5 cut(s) 115, 986, 1108, 1418, 1538
RsaNI GTAC 5 cut(s) 114, 985, 1107, 1417, 1537
SacI GAGCTC 1 cut(s) 245
SalI GTCGAC 1 cut(s) 900
SapI GCTCTTC 1 cut(s) 813
SaqAI TTAA 4 cut(s) 695, 930, 1224, 1371
SatI GCNGC 5 cut(s) 504, 804, 1175, 1297, 1590
Sau3AI GATC 6 cut(s) 118, 559, 798, 817, 840, 1383
Sau96I GGNCC 3 cut(s) 255, 473, 1186
ScaI AGTACT 2 cut(s) 1108, 1418
SchI GAGTC 3 cut(s) 161, 611, 1345
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 2 cut(s) 245, 596
SfaNI GCATC 8 cut(s) 352, 411, 490, 544, 623, 695, 763, 1312
SinI GGWCC 3 cut(s) 255, 473, 1186
SmlI CTYRAG 3 cut(s) 728, 1258, 1370
SmoI CTYRAG 3 cut(s) 728, 1258, 1370
SnaBI TACGTA 1 cut(s) 113
SseBI AGGCCT 1 cut(s) 446
SsiI CCGC 5 cut(s) 71, 370, 803, 1352, 1639
SspI AATATT 1 cut(s) 780
SspMI CTAG 3 cut(s) 485, 575, 821
SstI GAGCTC 1 cut(s) 245
StuI AGGCCT 1 cut(s) 446
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 1 cut(s) 1504
TaaI ACNGT 3 cut(s) 412, 455, 1636
TaiI ACGT 4 cut(s) 115, 315, 385, 865
TaqI TCGA 6 cut(s) 427, 831, 858, 901, 1161, 1278
TatI WGTACW 4 cut(s) 984, 1106, 1416, 1536
TauI GCSGC 1 cut(s) 806
TfiI GAWTC 4 cut(s) 853, 1122, 1158, 1250
Tru1I TTAA 4 cut(s) 695, 930, 1224, 1371
Tru9I TTAA 4 cut(s) 695, 930, 1224, 1371
TscAI CASTG 4 cut(s) 69, 232, 762, 831
TseFI GTSAC 3 cut(s) 153, 1034, 1484
TseI GCWGC 4 cut(s) 503, 1174, 1296, 1589
Tsp45I GTSAC 3 cut(s) 153, 1034, 1484
TspDTI ATGAA 7 cut(s) 184, 700, 962, 1085, 1231, 1461, 1594
TspGWI ACGGA 2 cut(s) 717, 1199
TspRI CASTG 4 cut(s) 69, 232, 762, 831
Van91I CCANNNNNTGG 1 cut(s) 1566
Vha464I CTTAAG 1 cut(s) 1370
VneI GTGCAC 1 cut(s) 592
VpaK11BI GGWCC 3 cut(s) 255, 473, 1186
VspI ATTAAT 1 cut(s) 695
XapI RAATTY 5 cut(s) 101, 290, 624, 1013, 1227
XbaI TCTAGA 1 cut(s) 574
XmiI GTMKAC 3 cut(s) 109, 901, 1438
XmnI GAANNNNTTC 2 cut(s) 363, 1463
XspI CTAG 3 cut(s) 485, 575, 821
ZrmI AGTACT 2 cut(s) 1108, 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.