Rorug03G0065800

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0016364
Physical Location & Seq
Forward (+)
4863494 .. 4865845
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0065800.1

Sequence Viewer

Length: 1308 bp
ATGGCTTCAACAACTGAACCCAAAGGAGAAATCCAAGAAGTATCTGAAGATGTTTCTACTGAAACTCTCAAATACAAGACTTGGGTATTGAAGGTCTCCATTCACTGTGGTGCTTGCAGCAAGAAGGTCACCAAAATCTTGAAGAAAATTGATGGGGTTTACATTACACAGGTCGACTATTTGCAGCAAAAAGTGACGGTCATTGGGAATGTGGAGGCTGAAACTCTGATCAAGACACTGACCAAGAAAACAACGAAACACGTTGAGTTGTTGTGGCCTGACCCGAAAGACAATTCCAAAGACAAGAAACAGAGTAAACCCAAAGGCAATGAGAAACAGAGTGAAATCTCCGAGGAAGCAAAAGCAAACCACGGCGGCGGCGGCGGAGATAAGAAAAAGGGTAAAGAGCCGGTGAAGGATGAAGTTGTTGAAGTGCAAGAGTCTGGTGGGGGTAAGAAGAACTTTGAAGTTGGTAGTTCTAGCAATAGTAAAGGTGTTGAGGAAGGTAATGTTGTCAAACCACATGAGGGTGGCGGCGGTGGTGGGGAAGGAAAATTAAAGGAGGCACAGACTGGCAGCTTGCCCAGCCAGTCACAGCCGGCGGCGGCTGAAAAGCAGAGTGGGGGTGTTGAGAGTGAAGCTGTGGGGGAGACAAGTGGAGGTGGTGCTGGCAGTGGCAGTGGTGGTGGGGGTGGGGGTAAAAAGAAAAAGGGCAAGGGGCAGAACGGGAATCACAATGTCAATGCTGCTGAAGGTGAACTGGTTGTCAATGCACCAAGCACTGATTCACCACCACCGGTTCAGCCCGGGCCTCAGCCTCAGCCTCAGCCTCATGGGCCGCCTAATGTTCAGAGCCAAGTGCCCAATGTGCAGAGGCCTATTCCAGTTCCAGCCTCTCATGGCCCGCCACCTCAGCAGCAGGTGTATGAGCACCCGTACTACCCAAGGCAGCAGCAGCAGTATGAGCATCCTTACCCAAGGCAGCAGCACTATGAGCACCCTTACCCAAGGCAGCAGCAGCAGTACTTTACTCCTGTGCATGCGATGAACTACAATGCGGCACACCCCAGCATTAGCTACGGTGCGGCTCGCTATGCTCCACAACCTGTTTATTCTCAGTCGCACACTTATGCACATTGGGGTTTGGGGAATCAGGTTGATCCACCAGCCTATGATTTGGACAACCTTCCACCTGCGGTGCCATCCCATCCTCCACAGGCCTATCCACCACATCCTTACTATTCCTCTGATTCCGATAACTATGCATTCTTCAGTGATGAAAATCCAAATGGGTGCAGTATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

435

Amino Acids

46.69

Weight (kDa)

6.63

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 30 - 82 3.1e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 910, 1201
Acc36I ACCTGC 2 cut(s) 910, 1201
AccB1I GGYRCC 1 cut(s) 1198
AccI GTMKAC 1 cut(s) 174
AclWI GGATC 1 cut(s) 1154
AcuI CTGAAG 3 cut(s) 66, 771, 1255
AfaI GTAC 2 cut(s) 938, 1025
AfiI CCNNNNNNNGG 1 cut(s) 527
AflIII ACRYGT 1 cut(s) 259
AgeI ACCGGT 1 cut(s) 796
AgsI TTSAA 5 cut(s) 9, 91, 142, 431, 467
AjuI GAANNNNNNNTTGG 2 cut(s) 769, 801
AleI CACNNNNGTG 1 cut(s) 108
AluBI AGCT 3 cut(s) 579, 641, 1077
AluI AGCT 3 cut(s) 579, 641, 1077
Alw21I GWGCWC 2 cut(s) 933, 999
Alw26I GTCTC 2 cut(s) 100, 644
AlwI GGATC 1 cut(s) 1154
Ama87I CYCGRG 1 cut(s) 806
AoxI GGCC 6 cut(s) 275, 809, 836, 875, 901, 1218
AsiGI ACCGGT 1 cut(s) 796
AspS9I GGNCC 3 cut(s) 809, 836, 902
AsuC2I CCSGG 2 cut(s) 807, 808
AsuHPI GGTGA 4 cut(s) 121, 424, 767, 780
AvaI CYCGRG 1 cut(s) 806
BaeGI GKGCMC 1 cut(s) 864
BanI GGYRCC 1 cut(s) 1198
Bbv12I GWGCWC 2 cut(s) 933, 999
BbvCI CCTCAGC 4 cut(s) 813, 819, 825, 912
BccI CCATC 3 cut(s) 146, 1210, 1215
BceAI ACGGC 1 cut(s) 388
BcgI CGANNNNNNTGC 2 cut(s) 1244, 1278
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 2 cut(s) 807, 808
BcoDI GTCTC 2 cut(s) 100, 644
BfaI CTAG 1 cut(s) 480
BfuAI ACCTGC 2 cut(s) 910, 1201
BglI GCCNNNNNGGC 1 cut(s) 835
BmcAI AGTACT 1 cut(s) 1025
Bme1390I CCNGG 2 cut(s) 807, 808
BmeT110I CYCGRG 1 cut(s) 806
BmgT120I GGNCC 3 cut(s) 809, 836, 902
BmiI GGNNCC 1 cut(s) 1200
BmrFI CCNGG 2 cut(s) 807, 808
BmsI GCATC 1 cut(s) 976
Bpu10I CCTNAGC 4 cut(s) 813, 819, 825, 912
BpuMI CCSGG 2 cut(s) 807, 808
BsaBI GATNNNNATC 1 cut(s) 1281
BsaI GGTCTC 1 cut(s) 100
BsaJI CCNNGG 6 cut(s) 351, 370, 806, 944, 977, 1007
BsaWI WCCGGW 1 cut(s) 796
Bsc4I CCNNNNNNNGG 1 cut(s) 527
Bse118I RCCGGY 3 cut(s) 409, 598, 796
Bse1I ACTGG 4 cut(s) 577, 589, 765, 884
Bse3DI GCAATG 1 cut(s) 334
Bse8I GATNNNNATC 1 cut(s) 1281
BseDI CCNNGG 6 cut(s) 351, 370, 806, 944, 977, 1007
BseGI GGATG 5 cut(s) 424, 967, 1202, 1207, 1231
BseJI GATNNNNATC 1 cut(s) 1281
BseLI CCNNNNNNNGG 1 cut(s) 527
BseMI GCAATG 1 cut(s) 334
BseMII CTCAG 5 cut(s) 827, 833, 839, 926, 1130
BseNI ACTGG 4 cut(s) 577, 589, 765, 884
BseSI GKGCMC 1 cut(s) 864
BseYI CCCAGC 2 cut(s) 584, 1067
BsgI GTGCAG 1 cut(s) 890
BshFI GGCC 6 cut(s) 277, 811, 838, 877, 903, 1220
BshNI GGYRCC 1 cut(s) 1198
BshTI ACCGGT 1 cut(s) 796
BsiHKAI GWGCWC 2 cut(s) 933, 999
BsiHKCI CYCGRG 1 cut(s) 806
BsiSI CCGG 4 cut(s) 410, 599, 797, 807
BslI CCNNNNNNNGG 1 cut(s) 527
BsmAI GTCTC 2 cut(s) 100, 644
BsmI GAATGC 1 cut(s) 1265
BsnI GGCC 6 cut(s) 277, 811, 838, 877, 903, 1220
Bso31I GGTCTC 1 cut(s) 100
BsoBI CYCGRG 1 cut(s) 806
Bsp1286I GDGCHC 3 cut(s) 864, 933, 999
Bsp143I GATC 2 cut(s) 228, 1159
BspANI GGCC 6 cut(s) 277, 811, 838, 877, 903, 1220
BspCNI CTCAG 5 cut(s) 826, 832, 838, 925, 1129
BspLI GGNNCC 1 cut(s) 1200
BspMI ACCTGC 2 cut(s) 910, 1201
BspPI GGATC 1 cut(s) 1154
BspT107I GGYRCC 1 cut(s) 1198
BspTNI GGTCTC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 334
BsrFI RCCGGY 3 cut(s) 409, 598, 796
BsrI ACTGG 4 cut(s) 577, 589, 765, 884
BssAI RCCGGY 3 cut(s) 409, 598, 796
BssECI CCNNGG 6 cut(s) 351, 370, 806, 944, 977, 1007
BssMI GATC 2 cut(s) 228, 1159
BssT1I CCWWGG 3 cut(s) 944, 977, 1007
Bst4CI ACNGT 3 cut(s) 107, 199, 1082
BstC8I GCNNGC 7 cut(s) 115, 581, 600, 670, 905, 1041, 1090
BstDEI CTNAG 5 cut(s) 813, 819, 825, 912, 1116
BstDSI CCRYGG 1 cut(s) 370
BstEII GGTNACC 1 cut(s) 127
BstF5I GGATG 5 cut(s) 424, 967, 1202, 1207, 1231
BstKTI GATC 2 cut(s) 231, 1162
BstMAI GTCTC 2 cut(s) 100, 644
BstMBI GATC 2 cut(s) 228, 1159
BstNSI RCATGY 1 cut(s) 1043
BstPI GGTNACC 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 805, 806
BstSLI GKGCMC 1 cut(s) 864
BsuRI GGCC 6 cut(s) 277, 811, 838, 877, 903, 1220
BtgI CCRYGG 1 cut(s) 370
BtgZI GCGATG 1 cut(s) 1058
BtsCI GGATG 5 cut(s) 424, 967, 1202, 1207, 1231
BtsI GCAGTG 2 cut(s) 679, 685
BtsIMutI CAGTG 6 cut(s) 103, 236, 679, 685, 780, 1279
BveI ACCTGC 2 cut(s) 910, 1201
Cac8I GCNNGC 7 cut(s) 115, 581, 600, 670, 905, 1041, 1090
Cfr10I RCCGGY 3 cut(s) 409, 598, 796
Cfr13I GGNCC 3 cut(s) 809, 836, 902
Cfr9I CCCGGG 1 cut(s) 806
Csp6I GTAC 2 cut(s) 937, 1024
CspAI ACCGGT 1 cut(s) 796
CviAII CATG 5 cut(s) 524, 833, 899, 1040, 1303
CviQI GTAC 2 cut(s) 937, 1024
DdeI CTNAG 5 cut(s) 813, 819, 825, 912, 1116
DpnI GATC 2 cut(s) 230, 1161
DpnII GATC 2 cut(s) 228, 1159
EciI GGCGGA 1 cut(s) 399
Eco130I CCWWGG 3 cut(s) 944, 977, 1007
Eco147I AGGCCT 2 cut(s) 877, 1220
Eco31I GGTCTC 1 cut(s) 100
Eco57I CTGAAG 3 cut(s) 66, 771, 1255
Eco88I CYCGRG 1 cut(s) 806
Eco91I GGTNACC 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 127
EcoT14I CCWWGG 3 cut(s) 944, 977, 1007
EcoT22I ATGCAT 1 cut(s) 1267
ErhI CCWWGG 3 cut(s) 944, 977, 1007
FaeI CATG 5 cut(s) 527, 836, 902, 1043, 1306
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FatI CATG 5 cut(s) 523, 832, 898, 1039, 1302
FauI CCCGC 1 cut(s) 912
FbaI TGATCA 1 cut(s) 228
FblI GTMKAC 1 cut(s) 174
FokI GGATG 5 cut(s) 431, 954, 1189, 1194, 1218
FspBI CTAG 1 cut(s) 480
GsaI CCCAGC 2 cut(s) 588, 1071
HaeIII GGCC 6 cut(s) 277, 811, 838, 877, 903, 1220
HapII CCGG 4 cut(s) 410, 599, 797, 807
Hin1II CATG 5 cut(s) 527, 836, 902, 1043, 1306
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 5 cut(s) 440, 730, 785, 1150, 1250
HpaII CCGG 4 cut(s) 410, 599, 797, 807
HphI GGTGA 4 cut(s) 121, 424, 767, 780
Hpy166II GTNNAC 4 cut(s) 160, 175, 317, 758
Hpy188I TCNGA 6 cut(s) 46, 228, 352, 852, 1249, 1255
Hpy188III TCNNGA 2 cut(s) 139, 232
Hpy8I GTNNAC 4 cut(s) 160, 175, 317, 758
HpyAV CCTTC 7 cut(s) 85, 118, 409, 497, 542, 746, 1196
HpyCH4III ACNGT 3 cut(s) 107, 199, 1082
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 9 cut(s) 117, 184, 436, 773, 871, 1039, 1133, 1265, 1296
HpyF3I CTNAG 5 cut(s) 813, 819, 825, 912, 1116
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 5 cut(s) 527, 836, 902, 1043, 1306
KroI GCCGGC 1 cut(s) 598
KroNI GCCGGC 1 cut(s) 600
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 2 cut(s) 228, 1159
LmnI GCTCC 1 cut(s) 1102
LweI GCATC 1 cut(s) 976
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 3 cut(s) 127, 193, 591
MalI GATC 2 cut(s) 230, 1161
MboI GATC 2 cut(s) 228, 1159
MboII GAAGA 4 cut(s) 59, 154, 469, 1261
MhlI GDGCHC 3 cut(s) 864, 933, 999
MluCI AATT 3 cut(s) 147, 292, 554
MlyI GAGTC 1 cut(s) 449
Mph1103I ATGCAT 1 cut(s) 1267
MroNI GCCGGC 1 cut(s) 598
MseI TTAA 1 cut(s) 557
MslI CAYNNNNRTG 3 cut(s) 108, 528, 1128
MspI CCGG 4 cut(s) 410, 599, 797, 807
MspR9I CCNGG 2 cut(s) 807, 808
Mva1269I GAATGC 1 cut(s) 1265
NaeI GCCGGC 1 cut(s) 600
NciI CCSGG 2 cut(s) 807, 808
NdeII GATC 2 cut(s) 228, 1159
NgoMIV GCCGGC 1 cut(s) 598
NlaIII CATG 5 cut(s) 527, 836, 902, 1043, 1306
NlaIV GGNNCC 1 cut(s) 1200
NmuCI GTSAC 3 cut(s) 127, 193, 591
NsiI ATGCAT 1 cut(s) 1267
NspI RCATGY 1 cut(s) 1043
OliI CACNNNNGTG 1 cut(s) 108
PaeI GCATGC 1 cut(s) 1043
PaqCI CACCTGC 2 cut(s) 910, 1201
PceI AGGCCT 2 cut(s) 877, 1220
PctI GAATGC 1 cut(s) 1265
PdiI GCCGGC 1 cut(s) 600
PfeI GAWTC 4 cut(s) 730, 785, 1150, 1250
PinAI ACCGGT 1 cut(s) 796
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PspEI GGTNACC 1 cut(s) 127
PspFI CCCAGC 2 cut(s) 584, 1067
PspN4I GGNNCC 1 cut(s) 1200
PspPI GGNCC 3 cut(s) 809, 836, 902
RsaI GTAC 2 cut(s) 938, 1025
RsaNI GTAC 2 cut(s) 937, 1024
RseI CAYNNNNRTG 3 cut(s) 108, 528, 1128
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 557
Sau3AI GATC 2 cut(s) 228, 1159
Sau96I GGNCC 3 cut(s) 809, 836, 902
ScaI AGTACT 1 cut(s) 1025
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 2 cut(s) 807, 808
SduI GDGCHC 3 cut(s) 864, 933, 999
SfaNI GCATC 1 cut(s) 976
SmaI CCCGGG 1 cut(s) 808
SmiMI CAYNNNNRTG 3 cut(s) 108, 528, 1128
SphI GCATGC 1 cut(s) 1043
SrfI GCCCGGGC 1 cut(s) 808
Sse9I AATT 3 cut(s) 147, 292, 554
SseBI AGGCCT 2 cut(s) 877, 1220
SspMI CTAG 1 cut(s) 480
StuI AGGCCT 2 cut(s) 877, 1220
StyD4I CCNGG 2 cut(s) 805, 806
StyI CCWWGG 3 cut(s) 944, 977, 1007
TaaI ACNGT 3 cut(s) 107, 199, 1082
TaiI ACGT 1 cut(s) 264
TaqI TCGA 1 cut(s) 174
TasI AATT 3 cut(s) 147, 292, 554
TatI WGTACW 1 cut(s) 1023
TauI GCSGC 9 cut(s) 378, 381, 384, 537, 605, 608, 841, 1061, 1088
TfiI GAWTC 4 cut(s) 730, 785, 1150, 1250
Tru1I TTAA 1 cut(s) 557
Tru9I TTAA 1 cut(s) 557
TscAI CASTG 6 cut(s) 110, 243, 679, 685, 787, 1279
TseFI GTSAC 3 cut(s) 127, 193, 591
Tsp45I GTSAC 3 cut(s) 127, 193, 591
TspDTI ATGAA 3 cut(s) 435, 1061, 1293
TspMI CCCGGG 1 cut(s) 806
TspRI CASTG 6 cut(s) 110, 243, 679, 685, 787, 1279
XceI RCATGY 1 cut(s) 1043
XmaI CCCGGG 1 cut(s) 806
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 480
ZrmI AGTACT 1 cut(s) 1025
Zsp2I ATGCAT 1 cut(s) 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.