Rroxscaffold_6G00416470

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0016364
Physical Location & Seq
Forward (+)
38511106 .. 38513350
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00416470.1

Sequence Viewer

Length: 1368 bp
ATGACTGAATTCCAGCAGGAAATCCAGCAAGTAGAAGCAATTGACAACAAGAAAGAGTGGCATCGGGAAATCACTGAAAAGCTTCACCGCAAGGTGAAAAGTGGTTCTGGATGTCTGAGCATCCACACGGTTCCAACGAGACTTCGACGAGTGAAGGATGAGGCCTACAACTGTCGCATTGTCTCAATTGGTCCTTTGCATCTAGGCAACCATGACCTTGCAGCCATGAGAGAGCGTAAATGGGCCTACATGCATCACTTATTTCAGCGAATTGAAGATCGTCAGCAGTTTCTAGAGTGTTGGAAAGAGTGCACGAGTGCTGTTTATGATTTAGACTCAGAAATTCGTGGATGCTATGCTAAAAGGATGAGCCACAAAGACAAGGGACACAAGGAGCTTGCAGAAGTTGTGCTAGTTGATGGATGCTTCATTCTGGAACTTTTCCTCAAGTATGAGGATCACTTGAATATGACACTGGATTATCAATCTGATGTAATATTCAATAGTGCTTGGATGATCGCGGCTCTTCGGCATGACCTAGCACTACTGGAAAACCAGATCCCCTTTATGATTCTCGAACGTTTATATGAGGCCATTAAGCCTCATCTAAAGAATAGTCGACCAAACTCTGTCGCTAGCCTTGCTCTTACTTTTTTTCAACCCATGAACTATAAGGCCTTTTTAGAAGATTGGGACACGCAGTACAACCATTTGCTTGATGTCTTGCACAAATTTTACCTCCCAAGTAATTGTGACATCTCAGTAGAAATTGAAATGATTACCGAAATGAAACATCATTCCAGACCTGAACCAGTATTCGGCAGTACTAAAAAATGGGGATTCAATTTTTGTGCTTCAGAGCTTTCGGACGCTGGAATCGAGTTTCAGAAGCAGCAGAGGACGGACCATTTGTTGAACATAACTTTCAGCAGGGGAGTAATTAAAATTCCACCGATTTTCATTGACGATTCACTCTTGAGGAACTTGATTGCTTTCGAGCAATGCAGTCTTGGCAGCCTGCATCACATGACATCATACGCAATTCTCTTGAAGAGTCTCATTCGTTACCCGCGAGATATCAGACTACTTAAGAAAAGGGGGATCATATGTTCCAATTGGATTAGAGAAGAAGAGTACTTGTCTCAACTCAAAAGTATACTTGATGAAGTTGTTGTGAAGGAGTTCTACTTTGGTGATTTGTGTGACAAAGTGAATGCTTACCATGGCAAGTTCTGGTTTCGCAGGGGTATAAAGTACATTTATCGCACTTATTTCTCCACAGCATGGAGTATGATATCATTTGTTGCAGCCATTATACTTTTCATTCTCACAGCTTTACAAACATTCTTTACTGTCCGCCCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

455

Amino Acids

53.64

Weight (kDa)

8.19

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 41 - 443 1.3e-95 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1284
AccI GTMKAC 2 cut(s) 619, 1156
AccII CGCG 2 cut(s) 521, 1072
AciI CCGC 4 cut(s) 88, 521, 1070, 1357
AclI AACGTT 1 cut(s) 580
AclWI GGATC 3 cut(s) 465, 553, 1109
AcsI RAATTY 4 cut(s) 8, 342, 731, 945
AcuI CTGAAG 1 cut(s) 840
AfaI GTAC 4 cut(s) 704, 826, 1136, 1256
AfiI CCNNNNNNNGG 2 cut(s) 818, 1284
AflII CTTAAG 1 cut(s) 1088
AgsI TTSAA 8 cut(s) 275, 466, 502, 659, 773, 844, 916, 1051
AloI GAACNNNNNNTCC 2 cut(s) 1093, 1125
AluBI AGCT 4 cut(s) 82, 397, 862, 1334
AluI AGCT 4 cut(s) 82, 397, 862, 1334
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 4 cut(s) 133, 187, 1061, 1146
Alw44I GTGCAC 1 cut(s) 310
AlwI GGATC 3 cut(s) 465, 553, 1109
AoxI GGCC 4 cut(s) 162, 243, 591, 675
ApaLI GTGCAC 1 cut(s) 310
ApeKI GCWGC 4 cut(s) 221, 892, 1014, 1307
ApoI RAATTY 4 cut(s) 8, 342, 731, 945
ArsI GACNNNNNNTTYG 2 cut(s) 892, 924
Asp700I GAANNNNTTC 2 cut(s) 81, 1181
AspS9I GGNCC 3 cut(s) 191, 243, 904
AsuHPI GGTGA 3 cut(s) 77, 106, 1205
AsuNHI GCTAGC 1 cut(s) 635
AvaII GGWCC 2 cut(s) 191, 904
BaeGI GKGCMC 1 cut(s) 314
BarI GAAGNNNNNNTAC 2 cut(s) 1169, 1201
BauI CACGAG 1 cut(s) 313
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 4 cut(s) 233, 904, 1026, 1319
BccI CCATC 1 cut(s) 413
BcoDI GTCTC 4 cut(s) 133, 187, 1061, 1146
BfaI CTAG 5 cut(s) 203, 293, 413, 539, 636
BfrI CTTAAG 1 cut(s) 1088
BisI GCNGC 5 cut(s) 222, 522, 893, 1015, 1308
BlsI GCNGC 5 cut(s) 223, 523, 894, 1016, 1309
BmcAI AGTACT 2 cut(s) 826, 1136
Bme18I GGWCC 2 cut(s) 191, 904
BmgT120I GGNCC 3 cut(s) 191, 243, 904
BmiI GGNNCC 1 cut(s) 132
BmsI GCATC 7 cut(s) 70, 129, 208, 262, 341, 413, 1030
BmtI GCTAGC 1 cut(s) 639
BpuEI CTTGAG 2 cut(s) 431, 997
BsaJI CCNNGG 1 cut(s) 1222
Bsc4I CCNNNNNNNGG 2 cut(s) 818, 1284
Bse1I ACTGG 3 cut(s) 480, 552, 812
Bse3DI GCAATG 1 cut(s) 1007
BseDI CCNNGG 1 cut(s) 1222
BseGI GGATG 7 cut(s) 116, 120, 163, 356, 372, 428, 519
BseLI CCNNNNNNNGG 2 cut(s) 818, 1284
BseMI GCAATG 1 cut(s) 1007
BseMII CTCAG 3 cut(s) 107, 351, 774
BseNI ACTGG 3 cut(s) 480, 552, 812
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 4 cut(s) 233, 904, 1026, 1319
Bsh1236I CGCG 2 cut(s) 521, 1072
BshFI GGCC 4 cut(s) 164, 245, 593, 677
BsiHKAI GWGCWC 1 cut(s) 314
BslFI GGGAC 2 cut(s) 399, 707
BslI CCNNNNNNNGG 2 cut(s) 818, 1284
BsmAI GTCTC 4 cut(s) 133, 187, 1061, 1146
BsmFI GGGAC 2 cut(s) 399, 707
BsmI GAATGC 1 cut(s) 1219
BsnI GGCC 4 cut(s) 164, 245, 593, 677
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 5 cut(s) 277, 457, 516, 558, 1101
Bsp19I CCATGG 1 cut(s) 1222
BspACI CCGC 4 cut(s) 88, 521, 1070, 1357
BspANI GGCC 4 cut(s) 164, 245, 593, 677
BspCNI CTCAG 3 cut(s) 108, 350, 773
BspFNI CGCG 2 cut(s) 521, 1072
BspLI GGNNCC 1 cut(s) 132
BspOI GCTAGC 1 cut(s) 639
BspPI GGATC 3 cut(s) 465, 553, 1109
BspQI GCTCTTC 1 cut(s) 531
BspTI CTTAAG 1 cut(s) 1088
BsrDI GCAATG 1 cut(s) 1007
BsrI ACTGG 3 cut(s) 480, 552, 812
BssECI CCNNGG 1 cut(s) 1222
BssMI GATC 5 cut(s) 277, 457, 516, 558, 1101
BssNAI GTATAC 1 cut(s) 1157
BssSI CACGAG 1 cut(s) 313
BssT1I CCWWGG 1 cut(s) 1222
Bst1107I GTATAC 1 cut(s) 1157
Bst2BI CACGAG 1 cut(s) 313
Bst4CI ACNGT 3 cut(s) 130, 173, 1354
Bst6I CTCTTC 3 cut(s) 531, 1046, 1125
BstAFI CTTAAG 1 cut(s) 1088
BstC8I GCNNGC 3 cut(s) 399, 637, 1019
BstDEI CTNAG 3 cut(s) 116, 337, 760
BstDSI CCRYGG 1 cut(s) 1222
BstF5I GGATG 7 cut(s) 116, 120, 163, 356, 372, 428, 519
BstFNI CGCG 2 cut(s) 521, 1072
BstKTI GATC 5 cut(s) 280, 460, 519, 561, 1104
BstMAI GTCTC 4 cut(s) 133, 187, 1061, 1146
BstMBI GATC 5 cut(s) 277, 457, 516, 558, 1101
BstMWI GCNNNNNNNGC 2 cut(s) 641, 1011
BstNSI RCATGY 1 cut(s) 253
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 2 cut(s) 521, 1072
BstV1I GCAGC 4 cut(s) 233, 904, 1026, 1319
BstX2I RGATCY 1 cut(s) 558
BstYI RGATCY 1 cut(s) 558
BstZ17I GTATAC 1 cut(s) 1157
BsuRI GGCC 4 cut(s) 164, 245, 593, 677
BtgI CCRYGG 1 cut(s) 1222
BtsCI GGATG 7 cut(s) 116, 120, 163, 356, 372, 428, 519
BtsIMutI CAGTG 2 cut(s) 72, 473
Cac8I GCNNGC 3 cut(s) 399, 637, 1019
Cfr13I GGNCC 3 cut(s) 191, 243, 904
CseI GACGC 1 cut(s) 878
Csp6I GTAC 4 cut(s) 703, 825, 1135, 1255
CviAII CATG 8 cut(s) 212, 226, 250, 533, 664, 1027, 1223, 1284
CviQI GTAC 4 cut(s) 703, 825, 1135, 1255
DdeI CTNAG 3 cut(s) 116, 337, 760
DpnI GATC 5 cut(s) 279, 459, 518, 560, 1103
DpnII GATC 5 cut(s) 277, 457, 516, 558, 1101
Eam1104I CTCTTC 3 cut(s) 531, 1046, 1125
EarI CTCTTC 3 cut(s) 531, 1046, 1125
EciI GGCGGA 1 cut(s) 1346
Eco130I CCWWGG 1 cut(s) 1222
Eco147I AGGCCT 2 cut(s) 164, 677
Eco32I GATATC 2 cut(s) 1078, 1296
Eco47I GGWCC 2 cut(s) 191, 904
Eco57I CTGAAG 1 cut(s) 840
EcoRI GAATTC 1 cut(s) 8
EcoRV GATATC 2 cut(s) 1078, 1296
EcoT14I CCWWGG 1 cut(s) 1222
EcoT22I ATGCAT 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 1222
FaeI CATG 8 cut(s) 215, 229, 253, 536, 667, 1030, 1226, 1287
FaqI GGGAC 2 cut(s) 399, 707
FatI CATG 8 cut(s) 211, 225, 249, 532, 663, 1026, 1222, 1283
FauI CCCGC 1 cut(s) 1077
FauNDI CATATG 1 cut(s) 1106
FblI GTMKAC 2 cut(s) 619, 1156
Fnu4HI GCNGC 5 cut(s) 222, 522, 893, 1015, 1308
FokI GGATG 7 cut(s) 107, 123, 170, 363, 379, 435, 526
Fsp4HI GCNGC 5 cut(s) 222, 522, 893, 1015, 1308
FspBI CTAG 5 cut(s) 203, 293, 413, 539, 636
GluI GCNGC 5 cut(s) 222, 522, 893, 1015, 1308
HaeIII GGCC 4 cut(s) 164, 245, 593, 677
HgaI GACGC 1 cut(s) 878
Hin1II CATG 8 cut(s) 215, 229, 253, 536, 667, 1030, 1226, 1287
HincII GTYRAC 1 cut(s) 620
HindII GTYRAC 1 cut(s) 620
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 6 cut(s) 335, 571, 840, 876, 968, 1054
HphI GGTGA 3 cut(s) 77, 106, 1205
Hpy166II GTNNAC 3 cut(s) 312, 620, 1157
Hpy188I TCNGA 7 cut(s) 117, 340, 490, 859, 868, 888, 1082
Hpy188III TCNNGA 8 cut(s) 65, 108, 293, 434, 575, 801, 976, 1048
Hpy8I GTNNAC 3 cut(s) 312, 620, 1157
Hpy99I CGWCG 1 cut(s) 150
HpyAV CCTTC 2 cut(s) 148, 1171
HpyCH4III ACNGT 3 cut(s) 130, 173, 1354
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 9 cut(s) 199, 221, 253, 312, 401, 727, 1005, 1021, 1307
HpyF10VI GCNNNNNNNGC 2 cut(s) 641, 1011
HpyF3I CTNAG 3 cut(s) 116, 337, 760
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 8 cut(s) 215, 229, 253, 536, 667, 1030, 1226, 1287
Kzo9I GATC 5 cut(s) 277, 457, 516, 558, 1101
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 1 cut(s) 394
Lsp1109I GCAGC 4 cut(s) 233, 904, 1026, 1319
LweI GCATC 7 cut(s) 70, 129, 208, 262, 341, 413, 1030
MaeI CTAG 5 cut(s) 203, 293, 413, 539, 636
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 3 cut(s) 752, 1064, 1202
MalI GATC 5 cut(s) 279, 459, 518, 560, 1103
MboI GATC 5 cut(s) 277, 457, 516, 558, 1101
MboII GAAGA 6 cut(s) 287, 518, 698, 1063, 1139, 1142
MfeI CAATTG 3 cut(s) 39, 186, 1114
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 1 cut(s) 314
MlyI GAGTC 2 cut(s) 329, 1063
MmeI TCCRAC 2 cut(s) 158, 281
MnlI CCTC 8 cut(s) 154, 448, 455, 583, 612, 749, 891, 972
Mph1103I ATGCAT 1 cut(s) 255
MroXI GAANNNNTTC 2 cut(s) 81, 1181
MseI TTAA 3 cut(s) 597, 942, 1089
MspCI CTTAAG 1 cut(s) 1088
MunI CAATTG 3 cut(s) 39, 186, 1114
Mva1269I GAATGC 1 cut(s) 1219
MvnI CGCG 2 cut(s) 521, 1072
MwoI GCNNNNNNNGC 2 cut(s) 641, 1011
NcoI CCATGG 1 cut(s) 1222
NdeI CATATG 1 cut(s) 1106
NdeII GATC 5 cut(s) 277, 457, 516, 558, 1101
NheI GCTAGC 1 cut(s) 635
NlaIII CATG 8 cut(s) 215, 229, 253, 536, 667, 1030, 1226, 1287
NlaIV GGNNCC 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 752, 1202
NsiI ATGCAT 1 cut(s) 255
NspI RCATGY 1 cut(s) 253
PceI AGGCCT 2 cut(s) 164, 677
PciSI GCTCTTC 1 cut(s) 531
PcsI WCGNNNNNNNCGW 3 cut(s) 134, 876, 1069
PctI GAATGC 1 cut(s) 1219
PdmI GAANNNNTTC 2 cut(s) 81, 1181
PfeI GAWTC 4 cut(s) 571, 840, 876, 968
PflMI CCANNNNNTGG 1 cut(s) 1284
PkrI GCNGC 5 cut(s) 223, 523, 894, 1016, 1309
PleI GAGTC 2 cut(s) 329, 1062
PpsI GAGTC 2 cut(s) 329, 1062
Psp1406I AACGTT 1 cut(s) 580
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 3 cut(s) 191, 243, 904
PsuI RGATCY 1 cut(s) 558
RsaI GTAC 4 cut(s) 704, 826, 1136, 1256
RsaNI GTAC 4 cut(s) 703, 825, 1135, 1255
SalI GTCGAC 1 cut(s) 618
SapI GCTCTTC 1 cut(s) 531
SaqAI TTAA 3 cut(s) 597, 942, 1089
SatI GCNGC 5 cut(s) 222, 522, 893, 1015, 1308
Sau3AI GATC 5 cut(s) 277, 457, 516, 558, 1101
Sau96I GGNCC 3 cut(s) 191, 243, 904
ScaI AGTACT 2 cut(s) 826, 1136
SchI GAGTC 2 cut(s) 329, 1063
SduI GDGCHC 1 cut(s) 314
SfaNI GCATC 7 cut(s) 70, 129, 208, 262, 341, 413, 1030
SinI GGWCC 2 cut(s) 191, 904
SmlI CTYRAG 3 cut(s) 446, 976, 1088
SmoI CTYRAG 3 cut(s) 446, 976, 1088
SseBI AGGCCT 2 cut(s) 164, 677
SsiI CCGC 4 cut(s) 88, 521, 1070, 1357
SspI AATATT 1 cut(s) 498
SspMI CTAG 5 cut(s) 203, 293, 413, 539, 636
StuI AGGCCT 2 cut(s) 164, 677
StyI CCWWGG 1 cut(s) 1222
TaaI ACNGT 3 cut(s) 130, 173, 1354
TaiI ACGT 1 cut(s) 583
TaqI TCGA 5 cut(s) 145, 576, 619, 879, 996
TatI WGTACW 4 cut(s) 702, 824, 1134, 1254
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 4 cut(s) 571, 840, 876, 968
Tru1I TTAA 3 cut(s) 597, 942, 1089
Tru9I TTAA 3 cut(s) 597, 942, 1089
TscAI CASTG 2 cut(s) 79, 480
TseFI GTSAC 2 cut(s) 752, 1202
TseI GCWGC 4 cut(s) 221, 892, 1014, 1307
Tsp45I GTSAC 2 cut(s) 752, 1202
TspDTI ATGAA 6 cut(s) 418, 680, 803, 949, 1179, 1312
TspGWI ACGGA 1 cut(s) 917
TspRI CASTG 2 cut(s) 79, 480
Van91I CCANNNNNTGG 1 cut(s) 1284
Vha464I CTTAAG 1 cut(s) 1088
VneI GTGCAC 1 cut(s) 310
VpaK11BI GGWCC 2 cut(s) 191, 904
XapI RAATTY 4 cut(s) 8, 342, 731, 945
XbaI TCTAGA 1 cut(s) 292
XceI RCATGY 1 cut(s) 253
XmiI GTMKAC 2 cut(s) 619, 1156
XmnI GAANNNNTTC 2 cut(s) 81, 1181
XspI CTAG 5 cut(s) 203, 293, 413, 539, 636
ZrmI AGTACT 2 cut(s) 826, 1136
Zsp2I ATGCAT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.