Rw0G010500

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00452
OG0016364
Physical Location & Seq
Forward (+)
10100 .. 12247
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010500.1

Sequence Viewer

Length: 1611 bp
ATGTGCCTACCATTCAACGACCTAAGTCAATTAAGTGAGCTTAGTTTGACAGATGAGAAAGCAAAGCATTCACGAAATAATCAGAAGTGGAGCCAGAATACAACTAATTTGGAGTCGCTTGTTCTAGGGTGTTCCTATTCTCATATATCCATCTTCATAGTTGGCTACAAATCAAAGCCGATATGCAGTGATTGGGGAAAAAGCTCAAATTGGTGGTCCGACGACAAAGGAAAACCTGCCAAGATGATTGAATTCCAGCAGGAAATCCAGCAAGTAGAAGAAATTGACAAGAAGAAAGAGTGGCATCGGGAGATTACTGAAAAGCTACACCGCAAGGTGGAAAGTGGTTCTGGATGTCTGAGCATCCACACGGTTCCAACGAGACTTCGACGAGTGAAGGATGAGGCCTACAACTGTCGCATTGTCTCAATTGGTCCTTTGCATCTAGGCAACCATGACCTTGCAGCCATGAGAGAGCGTAAATGGGCTTACATGCATCACTTATTTCAGCGAATTGAAGATCGTCAGCAGTTTCTAGAGTGTTGGAAAGAGTGCACGAGTGCTGTTTATGATTTAGACTCACAAATTCGTGGATGCTACGCTAAAAGGATGAGCCACAAAGACCAGGGACACAAGGAGCTTGCAGAAGTTGTATTAATTGACGGATGCTTCATTCTGGAACTTTTCCTCAGGTATGAGGAACACTTGAATATGACACTGGATTATCAATCTGATCCAATATTCAATAGTGCCTGGATGATCGCGGCTCTTCAGCATGATCTAGCACTACTCGAAAACCAGATCCCCTTTATGGTTCTCGAACGTTTATATGAGGCCATTAAGCCTCATCTAAAGAATAGTCGACCAAACTCTGTCGCTAGCCTTGCTCTTACTTTTTTTCAACCCATGCACTATAAGGCCTTTTTAGAAGATTGGGACACGCAGTACAACCATTTGCTTGATGTCTTGCACAAATTTTACCTCCCAAGTAATTGTGACATCTCAGTAGAAATTGAAATGATTACCGAAATGAAACATCATTCCAGACCTGAACCAGTATTCGGCAGTACTAAAAAATGGGGATTCAATTTCTGTGCTTCAGAGCTTTCGGAGGCTGGAATCGAGTTTCAGAAGCAGCAGAGGACGGACCATTTGTTGAACATAACTTTCAGCAGGGGAGTAATTAAAATTCCACCGATTTTCATTGACGATTCACTCTTGAGGAACTTGATTGCTTTCGAGCAATGCAGTCTTGGCAGCCTGCATCACATGACATCATACGCAATTCTCTTGAAGAGTCTCATTCGTTCCCCGCGAGATATCAAACTACTTAAGCAAAGAGGGATCATAAGTTCCAATTGGGTTAGAGAAGAAGAGTACTTGTCTCAACTCAAAAGTATACTTGATGAAGTTGTTGTGAAGGAGTTCTACTTTGGTGATTTGTGTGACAAAGTGAATGCTTACCATGGCAAGTTCTGGTTTCGCAGAGGTATAAAGTACATTTATCGCACTTATTTCTCCACAGCATGGAGTATGATATCATTTGTTGCAGCCATTATACTTTTCATTCTCACAGCTTTACAAACATTCTTTACTGTTCGCCCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

536

Amino Acids

62.8

Weight (kDa)

7.62

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 122 - 524 6.1e-98 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 244
AccB7I CCANNNNNTGG 1 cut(s) 1527
AccI GTMKAC 2 cut(s) 862, 1399
AccII CGCG 2 cut(s) 764, 1315
AciI CCGC 3 cut(s) 331, 764, 1313
AclI AACGTT 1 cut(s) 823
AclWI GGATC 3 cut(s) 728, 796, 1352
AcsI RAATTY 4 cut(s) 251, 585, 974, 1188
AcuI CTGAAG 2 cut(s) 755, 1083
AfaI GTAC 4 cut(s) 947, 1069, 1379, 1499
AfiI CCNNNNNNNGG 4 cut(s) 337, 811, 1061, 1527
AflII CTTAAG 1 cut(s) 1331
AjnI CCWGG 2 cut(s) 624, 752
AloI GAACNNNNNNTCC 2 cut(s) 1336, 1368
AluBI AGCT 6 cut(s) 40, 204, 325, 640, 1105, 1577
AluI AGCT 6 cut(s) 40, 204, 325, 640, 1105, 1577
Alw21I GWGCWC 1 cut(s) 557
Alw26I GTCTC 4 cut(s) 376, 430, 1304, 1389
Alw44I GTGCAC 1 cut(s) 553
AlwI GGATC 3 cut(s) 728, 796, 1352
AoxI GGCC 3 cut(s) 405, 834, 918
ApaLI GTGCAC 1 cut(s) 553
ApeKI GCWGC 4 cut(s) 464, 1135, 1257, 1550
ApoI RAATTY 4 cut(s) 251, 585, 974, 1188
ArsI GACNNNNNNTTYG 4 cut(s) 200, 232, 1135, 1167
AseI ATTAAT 1 cut(s) 656
Asp700I GAANNNNTTC 1 cut(s) 1424
AspS9I GGNCC 3 cut(s) 216, 434, 1147
AsuHPI GGTGA 1 cut(s) 1448
AsuNHI GCTAGC 1 cut(s) 878
AvaII GGWCC 3 cut(s) 216, 434, 1147
AxyI CCTNAGG 1 cut(s) 689
BaeGI GKGCMC 1 cut(s) 557
BarI GAAGNNNNNNTAC 2 cut(s) 1412, 1444
BauI CACGAG 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 557
BbvI GCAGC 4 cut(s) 476, 1147, 1269, 1562
BccI CCATC 1 cut(s) 158
BciT130I CCWGG 2 cut(s) 626, 754
BcoDI GTCTC 4 cut(s) 376, 430, 1304, 1389
BfaI CTAG 5 cut(s) 125, 446, 536, 782, 879
BfrI CTTAAG 1 cut(s) 1331
BfuAI ACCTGC 1 cut(s) 244
BisI GCNGC 5 cut(s) 465, 765, 1136, 1258, 1551
BlsI GCNGC 5 cut(s) 466, 766, 1137, 1259, 1552
BmcAI AGTACT 2 cut(s) 1069, 1379
Bme1390I CCNGG 2 cut(s) 626, 754
Bme18I GGWCC 3 cut(s) 216, 434, 1147
BmgT120I GGNCC 3 cut(s) 216, 434, 1147
BmiI GGNNCC 2 cut(s) 92, 375
BmrFI CCNGG 2 cut(s) 626, 754
BmsI GCATC 7 cut(s) 313, 372, 451, 505, 584, 656, 1273
BmtI GCTAGC 1 cut(s) 882
BoxI GACNNNNGTC 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 1240
BsaJI CCNNGG 2 cut(s) 625, 1465
Bsc4I CCNNNNNNNGG 4 cut(s) 337, 811, 1061, 1527
Bse1I ACTGG 2 cut(s) 723, 1055
Bse21I CCTNAGG 1 cut(s) 689
Bse3DI GCAATG 1 cut(s) 1250
BseBI CCWGG 2 cut(s) 626, 754
BseDI CCNNGG 2 cut(s) 625, 1465
BseGI GGATG 7 cut(s) 359, 363, 406, 599, 615, 671, 762
BseLI CCNNNNNNNGG 4 cut(s) 337, 811, 1061, 1527
BseMI GCAATG 1 cut(s) 1250
BseMII CTCAG 3 cut(s) 350, 703, 1017
BseNI ACTGG 2 cut(s) 723, 1055
BseSI GKGCMC 1 cut(s) 557
BseXI GCAGC 4 cut(s) 476, 1147, 1269, 1562
Bsh1236I CGCG 2 cut(s) 764, 1315
BshFI GGCC 3 cut(s) 407, 836, 920
BsiHKAI GWGCWC 1 cut(s) 557
BslFI GGGAC 2 cut(s) 642, 950
BslI CCNNNNNNNGG 4 cut(s) 337, 811, 1061, 1527
BsmAI GTCTC 4 cut(s) 376, 430, 1304, 1389
BsmFI GGGAC 2 cut(s) 642, 950
BsmI GAATGC 2 cut(s) 67, 1462
BsnI GGCC 3 cut(s) 407, 836, 920
Bsp1286I GDGCHC 1 cut(s) 557
Bsp143I GATC 6 cut(s) 520, 733, 759, 778, 801, 1344
Bsp19I CCATGG 1 cut(s) 1465
BspACI CCGC 3 cut(s) 331, 764, 1313
BspANI GGCC 3 cut(s) 407, 836, 920
BspCNI CTCAG 3 cut(s) 351, 702, 1016
BspFNI CGCG 2 cut(s) 764, 1315
BspLI GGNNCC 2 cut(s) 92, 375
BspMI ACCTGC 1 cut(s) 244
BspOI GCTAGC 1 cut(s) 882
BspPI GGATC 3 cut(s) 728, 796, 1352
BspQI GCTCTTC 1 cut(s) 774
BspTI CTTAAG 1 cut(s) 1331
BsrDI GCAATG 1 cut(s) 1250
BsrI ACTGG 2 cut(s) 723, 1055
BssECI CCNNGG 2 cut(s) 625, 1465
BssMI GATC 6 cut(s) 520, 733, 759, 778, 801, 1344
BssNAI GTATAC 1 cut(s) 1400
BssSI CACGAG 1 cut(s) 556
BssT1I CCWWGG 1 cut(s) 1465
Bst1107I GTATAC 1 cut(s) 1400
Bst2BI CACGAG 1 cut(s) 556
Bst2UI CCWGG 2 cut(s) 626, 754
Bst4CI ACNGT 3 cut(s) 373, 416, 1597
Bst6I CTCTTC 3 cut(s) 774, 1289, 1368
BstAFI CTTAAG 1 cut(s) 1331
BstC8I GCNNGC 3 cut(s) 642, 880, 1262
BstDEI CTNAG 5 cut(s) 23, 41, 359, 689, 1003
BstDSI CCRYGG 1 cut(s) 1465
BstF5I GGATG 7 cut(s) 359, 363, 406, 599, 615, 671, 762
BstFNI CGCG 2 cut(s) 764, 1315
BstKTI GATC 6 cut(s) 523, 736, 762, 781, 804, 1347
BstMAI GTCTC 4 cut(s) 376, 430, 1304, 1389
BstMBI GATC 6 cut(s) 520, 733, 759, 778, 801, 1344
BstMWI GCNNNNNNNGC 2 cut(s) 884, 1254
BstNI CCWGG 2 cut(s) 626, 754
BstNSI RCATGY 1 cut(s) 496
BstPAI GACNNNNGTC 1 cut(s) 24
BstSCI CCNGG 2 cut(s) 624, 752
BstSLI GKGCMC 1 cut(s) 557
BstUI CGCG 2 cut(s) 764, 1315
BstV1I GCAGC 4 cut(s) 476, 1147, 1269, 1562
BstX2I RGATCY 1 cut(s) 801
BstYI RGATCY 1 cut(s) 801
BstZ17I GTATAC 1 cut(s) 1400
Bsu36I CCTNAGG 1 cut(s) 689
BsuRI GGCC 3 cut(s) 407, 836, 920
BtgI CCRYGG 1 cut(s) 1465
BtsCI GGATG 7 cut(s) 359, 363, 406, 599, 615, 671, 762
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 2 cut(s) 193, 716
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 3 cut(s) 642, 880, 1262
Cfr13I GGNCC 3 cut(s) 216, 434, 1147
Csp6I GTAC 4 cut(s) 946, 1068, 1378, 1498
CviAII CATG 8 cut(s) 455, 469, 493, 776, 907, 1270, 1466, 1527
CviQI GTAC 4 cut(s) 946, 1068, 1378, 1498
DdeI CTNAG 5 cut(s) 23, 41, 359, 689, 1003
DpnI GATC 6 cut(s) 522, 735, 761, 780, 803, 1346
DpnII GATC 6 cut(s) 520, 733, 759, 778, 801, 1344
Eam1104I CTCTTC 3 cut(s) 774, 1289, 1368
EarI CTCTTC 3 cut(s) 774, 1289, 1368
Eco130I CCWWGG 1 cut(s) 1465
Eco147I AGGCCT 2 cut(s) 407, 920
Eco32I GATATC 2 cut(s) 1321, 1539
Eco47I GGWCC 3 cut(s) 216, 434, 1147
Eco57I CTGAAG 2 cut(s) 755, 1083
Eco81I CCTNAGG 1 cut(s) 689
EcoRI GAATTC 1 cut(s) 251
EcoRII CCWGG 2 cut(s) 624, 752
EcoRV GATATC 2 cut(s) 1321, 1539
EcoT14I CCWWGG 1 cut(s) 1465
EcoT22I ATGCAT 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 1465
FaeI CATG 8 cut(s) 458, 472, 496, 779, 910, 1273, 1469, 1530
FaqI GGGAC 2 cut(s) 642, 950
FatI CATG 8 cut(s) 454, 468, 492, 775, 906, 1269, 1465, 1526
FauI CCCGC 1 cut(s) 1320
FblI GTMKAC 2 cut(s) 862, 1399
Fnu4HI GCNGC 5 cut(s) 465, 765, 1136, 1258, 1551
FokI GGATG 7 cut(s) 350, 366, 413, 606, 622, 678, 769
Fsp4HI GCNGC 5 cut(s) 465, 765, 1136, 1258, 1551
FspBI CTAG 5 cut(s) 125, 446, 536, 782, 879
GluI GCNGC 5 cut(s) 465, 765, 1136, 1258, 1551
HaeIII GGCC 3 cut(s) 407, 836, 920
Hin1II CATG 8 cut(s) 458, 472, 496, 779, 910, 1273, 1469, 1530
HincII GTYRAC 1 cut(s) 863
HindII GTYRAC 1 cut(s) 863
HinfI GANTC 6 cut(s) 113, 578, 1083, 1119, 1211, 1297
HphI GGTGA 1 cut(s) 1448
Hpy166II GTNNAC 3 cut(s) 555, 863, 1400
Hpy188I TCNGA 7 cut(s) 84, 220, 360, 733, 1102, 1111, 1131
Hpy188III TCNNGA 9 cut(s) 72, 308, 351, 536, 677, 818, 1044, 1219, 1291
Hpy8I GTNNAC 3 cut(s) 555, 863, 1400
Hpy99I CGWCG 2 cut(s) 224, 393
HpyAV CCTTC 2 cut(s) 391, 1414
HpyCH4III ACNGT 3 cut(s) 373, 416, 1597
HpyCH4IV ACGT 1 cut(s) 823
HpyF10VI GCNNNNNNNGC 2 cut(s) 884, 1254
HpyF3I CTNAG 5 cut(s) 23, 41, 359, 689, 1003
HpySE526I ACGT 1 cut(s) 823
Hsp92II CATG 8 cut(s) 458, 472, 496, 779, 910, 1273, 1469, 1530
Kzo9I GATC 6 cut(s) 520, 733, 759, 778, 801, 1344
LguI GCTCTTC 1 cut(s) 774
LmnI GCTCC 2 cut(s) 90, 637
Lsp1109I GCAGC 4 cut(s) 476, 1147, 1269, 1562
LweI GCATC 7 cut(s) 313, 372, 451, 505, 584, 656, 1273
MaeI CTAG 5 cut(s) 125, 446, 536, 782, 879
MaeII ACGT 1 cut(s) 823
MaeIII GTNAC 2 cut(s) 995, 1445
MalI GATC 6 cut(s) 522, 735, 761, 780, 803, 1346
MboI GATC 6 cut(s) 520, 733, 759, 778, 801, 1344
MboII GAAGA 9 cut(s) 145, 290, 304, 530, 761, 941, 1306, 1382, 1385
MfeI CAATTG 2 cut(s) 429, 1357
MflI RGATCY 1 cut(s) 801
MhlI GDGCHC 1 cut(s) 557
MlyI GAGTC 3 cut(s) 122, 572, 1306
MmeI TCCRAC 3 cut(s) 243, 401, 524
Mph1103I ATGCAT 1 cut(s) 498
MroXI GAANNNNTTC 1 cut(s) 1424
MseI TTAA 5 cut(s) 32, 656, 840, 1185, 1332
MspCI CTTAAG 1 cut(s) 1331
MspR9I CCNGG 2 cut(s) 626, 754
MunI CAATTG 2 cut(s) 429, 1357
Mva1269I GAATGC 2 cut(s) 67, 1462
MvaI CCWGG 2 cut(s) 626, 754
MvnI CGCG 2 cut(s) 764, 1315
MwoI GCNNNNNNNGC 2 cut(s) 884, 1254
NcoI CCATGG 1 cut(s) 1465
NdeII GATC 6 cut(s) 520, 733, 759, 778, 801, 1344
NheI GCTAGC 1 cut(s) 878
NlaIII CATG 8 cut(s) 458, 472, 496, 779, 910, 1273, 1469, 1530
NlaIV GGNNCC 2 cut(s) 92, 375
NmuCI GTSAC 2 cut(s) 995, 1445
NsiI ATGCAT 1 cut(s) 498
NspI RCATGY 1 cut(s) 496
PceI AGGCCT 2 cut(s) 407, 920
PciSI GCTCTTC 1 cut(s) 774
PcsI WCGNNNNNNNCGW 2 cut(s) 377, 1312
PctI GAATGC 2 cut(s) 67, 1462
PdmI GAANNNNTTC 1 cut(s) 1424
PfeI GAWTC 3 cut(s) 1083, 1119, 1211
PflMI CCANNNNNTGG 1 cut(s) 1527
PkrI GCNGC 5 cut(s) 466, 766, 1137, 1259, 1552
PleI GAGTC 3 cut(s) 121, 572, 1305
PpsI GAGTC 3 cut(s) 121, 572, 1305
PshAI GACNNNNGTC 1 cut(s) 24
PshBI ATTAAT 1 cut(s) 656
Psp1406I AACGTT 1 cut(s) 823
Psp6I CCWGG 2 cut(s) 624, 752
PspGI CCWGG 2 cut(s) 624, 752
PspN4I GGNNCC 2 cut(s) 92, 375
PspPI GGNCC 3 cut(s) 216, 434, 1147
PsuI RGATCY 1 cut(s) 801
RsaI GTAC 4 cut(s) 947, 1069, 1379, 1499
RsaNI GTAC 4 cut(s) 946, 1068, 1378, 1498
SalI GTCGAC 1 cut(s) 861
SapI GCTCTTC 1 cut(s) 774
SaqAI TTAA 5 cut(s) 32, 656, 840, 1185, 1332
SatI GCNGC 5 cut(s) 465, 765, 1136, 1258, 1551
Sau3AI GATC 6 cut(s) 520, 733, 759, 778, 801, 1344
Sau96I GGNCC 3 cut(s) 216, 434, 1147
ScaI AGTACT 2 cut(s) 1069, 1379
SchI GAGTC 3 cut(s) 122, 572, 1306
ScrFI CCNGG 2 cut(s) 626, 754
SduI GDGCHC 1 cut(s) 557
SfaNI GCATC 7 cut(s) 313, 372, 451, 505, 584, 656, 1273
SinI GGWCC 3 cut(s) 216, 434, 1147
SmlI CTYRAG 2 cut(s) 1219, 1331
SmoI CTYRAG 2 cut(s) 1219, 1331
SseBI AGGCCT 2 cut(s) 407, 920
SsiI CCGC 3 cut(s) 331, 764, 1313
SspI AATATT 1 cut(s) 741
SspMI CTAG 5 cut(s) 125, 446, 536, 782, 879
StuI AGGCCT 2 cut(s) 407, 920
StyD4I CCNGG 2 cut(s) 624, 752
StyI CCWWGG 1 cut(s) 1465
TaaI ACNGT 3 cut(s) 373, 416, 1597
TaiI ACGT 1 cut(s) 826
TaqI TCGA 6 cut(s) 388, 792, 819, 862, 1122, 1239
TatI WGTACW 4 cut(s) 945, 1067, 1377, 1497
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 3 cut(s) 1083, 1119, 1211
Tru1I TTAA 5 cut(s) 32, 656, 840, 1185, 1332
Tru9I TTAA 5 cut(s) 32, 656, 840, 1185, 1332
TscAI CASTG 2 cut(s) 193, 723
TseFI GTSAC 2 cut(s) 995, 1445
TseI GCWGC 4 cut(s) 464, 1135, 1257, 1550
Tsp45I GTSAC 2 cut(s) 995, 1445
TspDTI ATGAA 6 cut(s) 145, 661, 1046, 1192, 1422, 1555
TspGWI ACGGA 2 cut(s) 678, 1160
TspRI CASTG 2 cut(s) 193, 723
Van91I CCANNNNNTGG 1 cut(s) 1527
Vha464I CTTAAG 1 cut(s) 1331
VneI GTGCAC 1 cut(s) 553
VpaK11BI GGWCC 3 cut(s) 216, 434, 1147
VspI ATTAAT 1 cut(s) 656
XapI RAATTY 4 cut(s) 251, 585, 974, 1188
XbaI TCTAGA 1 cut(s) 535
XceI RCATGY 1 cut(s) 496
XmiI GTMKAC 2 cut(s) 862, 1399
XmnI GAANNNNTTC 1 cut(s) 1424
XspI CTAG 5 cut(s) 125, 446, 536, 782, 879
ZrmI AGTACT 2 cut(s) 1069, 1379
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.