Rh3BG127900

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0016364
Physical Location & Seq
Reverse (-)
10713164 .. 10715525
2362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG127900.1

Sequence Viewer

Length: 1392 bp
ATGTGTATTCTGTCATATTTACATTCTGTCGCACGAAACAAATTCAAACAGCAAGTAGAAGAAATTGACAAGAAGAAAGAGTGGCATCGGGAGATTACTGAAAAGCTACACCGCAAGGTGGAAAGTGGTTCTGGATGTCTGAGCATCCACACGGTTCCAACGAGACTTCGACGAGTGAAGGATGAGGCCTACAACTGTCGCATTGTCTCAATTGGTCCTTTGCATCTAGGCAACCATGACCTTGCAGCCATGAGAGAGCGTAAATGGGCTTACATGCATCACTTATTTCAGCGAATTGAAGATCGTCAGCAGTTTCTAGAGTGTTGGAAAGAGTGCACGAGTGCTGTTTATGATTTAGACTCACAAATTCGTGGATGCTACGCTAAAAGGATGAGCCACAAAGACCAGGGACACAAGGAGCTTGCAGAAGTTGTATTAATTGACGGATGCTTCATTCTGGAACTTTTCCTCAGGTATGAGGAACACTTGAATATGACACTGGATTATCAATCTGATCCAATATTCAATAGTGCCTGGATGATCGCGGCTCTTCAGCATGATCTAGCACTACTCGAAAACCAGATCCCCTTTATGGTTCTCGAACGTTTATATGAGGCCATTAAGCCTCATCTAAAGAATAGTCAACCAAACTCTGTCGCTAGCCTTGCTCTTACTTTTTTTCAACCCATGCACTATAAGGCCTTTTTAGAAGATTGGGACACGCAGTACAACCATTTGCTTGATGTCTTGCACAAATTTTACCTCCCAAGTAATTGTGACATCTCAGTAGAAATTGAAATGATTACCGAAATGAAACATCATTCCAGACCTGAACCAGTATTCGGCAGTACTAAAAAATGGGGATTCAATTTCTGTGCTTCAGAGCTTTCGGAGGCTGGAATCGAGTTTCAGAAGCAGCAGAGGACGGACCATTTGTTGAACATAACTTTCAGCAGGGGAGTAATTAAAATTCCACCGATTTTCATTGACGATTCACTCTTGAGGAACTTGATTGCTTTCGAGCAATGCAGTCTTGGCAGCCTGCATCACATGACATCATACGCAATTCTCTTGAAGAGTCTCATTCGTTCCCCGCGAGATATCAAACTACTTAAGCAAAGAGGGATCATAAGTTCCAATTGGGTTAGAGAAGAAGAGTACTTGTCTCAACTCAAAAGTATACTTGATGAAGTTGTTGTGAAGGAGTTCTACTTTGGTGATTTGTGTGACAAAGTGAATGCTTACCATGGCAAGTTCTGGTTTCGCAGAGGTATAAAGTACATTTATCGCACTTATTTCTCCACAGCATGGAGTATGATATCATTTGTTGCAGCCATTATACTTTTCATTCTCACAGCTTTACAAACATTCTTTACTGTTCGCCCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

463

Amino Acids

54.48

Weight (kDa)

8.19

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 49 - 451 2.8e-98 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1308
AccI GTMKAC 1 cut(s) 1180
AccII CGCG 2 cut(s) 545, 1096
AciI CCGC 3 cut(s) 112, 545, 1094
AclI AACGTT 1 cut(s) 604
AclWI GGATC 3 cut(s) 509, 577, 1133
AcsI RAATTY 4 cut(s) 41, 366, 755, 969
AcuI CTGAAG 2 cut(s) 536, 864
AfaI GTAC 4 cut(s) 728, 850, 1160, 1280
AfiI CCNNNNNNNGG 4 cut(s) 118, 592, 842, 1308
AflII CTTAAG 1 cut(s) 1112
AgsI TTSAA 9 cut(s) 46, 299, 490, 526, 683, 797, 868, 940, 1075
AjnI CCWGG 2 cut(s) 405, 533
AloI GAACNNNNNNTCC 2 cut(s) 1117, 1149
AluBI AGCT 4 cut(s) 106, 421, 886, 1358
AluI AGCT 4 cut(s) 106, 421, 886, 1358
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 4 cut(s) 157, 211, 1085, 1170
Alw44I GTGCAC 1 cut(s) 334
AlwI GGATC 3 cut(s) 509, 577, 1133
AoxI GGCC 3 cut(s) 186, 615, 699
ApaLI GTGCAC 1 cut(s) 334
ApeKI GCWGC 4 cut(s) 245, 916, 1038, 1331
ApoI RAATTY 4 cut(s) 41, 366, 755, 969
ArsI GACNNNNNNTTYG 2 cut(s) 916, 948
AseI ATTAAT 1 cut(s) 437
Asp700I GAANNNNTTC 1 cut(s) 1205
AspS9I GGNCC 2 cut(s) 215, 928
AsuHPI GGTGA 1 cut(s) 1229
AsuNHI GCTAGC 1 cut(s) 659
AvaII GGWCC 2 cut(s) 215, 928
AxyI CCTNAGG 1 cut(s) 470
BaeGI GKGCMC 1 cut(s) 338
BarI GAAGNNNNNNTAC 2 cut(s) 1193, 1225
BauI CACGAG 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 338
BbvI GCAGC 4 cut(s) 257, 928, 1050, 1343
BciT130I CCWGG 2 cut(s) 407, 535
BcoDI GTCTC 4 cut(s) 157, 211, 1085, 1170
BfaI CTAG 4 cut(s) 227, 317, 563, 660
BfrI CTTAAG 1 cut(s) 1112
BisI GCNGC 5 cut(s) 246, 546, 917, 1039, 1332
BlsI GCNGC 5 cut(s) 247, 547, 918, 1040, 1333
BmcAI AGTACT 2 cut(s) 850, 1160
Bme1390I CCNGG 2 cut(s) 407, 535
Bme18I GGWCC 2 cut(s) 215, 928
BmgT120I GGNCC 2 cut(s) 215, 928
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 2 cut(s) 407, 535
BmsI GCATC 7 cut(s) 94, 153, 232, 286, 365, 437, 1054
BmtI GCTAGC 1 cut(s) 663
BpuEI CTTGAG 1 cut(s) 1021
BsaJI CCNNGG 2 cut(s) 406, 1246
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 592, 842, 1308
Bse1I ACTGG 2 cut(s) 504, 836
Bse21I CCTNAGG 1 cut(s) 470
Bse3DI GCAATG 1 cut(s) 1031
BseBI CCWGG 2 cut(s) 407, 535
BseDI CCNNGG 2 cut(s) 406, 1246
BseGI GGATG 7 cut(s) 140, 144, 187, 380, 396, 452, 543
BseLI CCNNNNNNNGG 4 cut(s) 118, 592, 842, 1308
BseMI GCAATG 1 cut(s) 1031
BseMII CTCAG 3 cut(s) 131, 484, 798
BseNI ACTGG 2 cut(s) 504, 836
BseSI GKGCMC 1 cut(s) 338
BseXI GCAGC 4 cut(s) 257, 928, 1050, 1343
Bsh1236I CGCG 2 cut(s) 545, 1096
BshFI GGCC 3 cut(s) 188, 617, 701
BsiHKAI GWGCWC 1 cut(s) 338
BslFI GGGAC 2 cut(s) 423, 731
BslI CCNNNNNNNGG 4 cut(s) 118, 592, 842, 1308
BsmAI GTCTC 4 cut(s) 157, 211, 1085, 1170
BsmFI GGGAC 2 cut(s) 423, 731
BsmI GAATGC 1 cut(s) 1243
BsnI GGCC 3 cut(s) 188, 617, 701
Bsp1286I GDGCHC 1 cut(s) 338
Bsp143I GATC 6 cut(s) 301, 514, 540, 559, 582, 1125
Bsp19I CCATGG 1 cut(s) 1246
BspACI CCGC 3 cut(s) 112, 545, 1094
BspANI GGCC 3 cut(s) 188, 617, 701
BspCNI CTCAG 3 cut(s) 132, 483, 797
BspFNI CGCG 2 cut(s) 545, 1096
BspLI GGNNCC 1 cut(s) 156
BspOI GCTAGC 1 cut(s) 663
BspPI GGATC 3 cut(s) 509, 577, 1133
BspQI GCTCTTC 1 cut(s) 555
BspTI CTTAAG 1 cut(s) 1112
BsrDI GCAATG 1 cut(s) 1031
BsrI ACTGG 2 cut(s) 504, 836
BssECI CCNNGG 2 cut(s) 406, 1246
BssMI GATC 6 cut(s) 301, 514, 540, 559, 582, 1125
BssNAI GTATAC 1 cut(s) 1181
BssSI CACGAG 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 1246
Bst1107I GTATAC 1 cut(s) 1181
Bst2BI CACGAG 1 cut(s) 337
Bst2UI CCWGG 2 cut(s) 407, 535
Bst4CI ACNGT 3 cut(s) 154, 197, 1378
Bst6I CTCTTC 3 cut(s) 555, 1070, 1149
BstAFI CTTAAG 1 cut(s) 1112
BstC8I GCNNGC 3 cut(s) 423, 661, 1043
BstDEI CTNAG 3 cut(s) 140, 470, 784
BstDSI CCRYGG 1 cut(s) 1246
BstF5I GGATG 7 cut(s) 140, 144, 187, 380, 396, 452, 543
BstFNI CGCG 2 cut(s) 545, 1096
BstKTI GATC 6 cut(s) 304, 517, 543, 562, 585, 1128
BstMAI GTCTC 4 cut(s) 157, 211, 1085, 1170
BstMBI GATC 6 cut(s) 301, 514, 540, 559, 582, 1125
BstMWI GCNNNNNNNGC 2 cut(s) 665, 1035
BstNI CCWGG 2 cut(s) 407, 535
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 405, 533
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 2 cut(s) 545, 1096
BstV1I GCAGC 4 cut(s) 257, 928, 1050, 1343
BstX2I RGATCY 1 cut(s) 582
BstYI RGATCY 1 cut(s) 582
BstZ17I GTATAC 1 cut(s) 1181
Bsu36I CCTNAGG 1 cut(s) 470
BsuRI GGCC 3 cut(s) 188, 617, 701
BtgI CCRYGG 1 cut(s) 1246
BtsCI GGATG 7 cut(s) 140, 144, 187, 380, 396, 452, 543
BtsIMutI CAGTG 1 cut(s) 497
Cac8I GCNNGC 3 cut(s) 423, 661, 1043
Cfr13I GGNCC 2 cut(s) 215, 928
Csp6I GTAC 4 cut(s) 727, 849, 1159, 1279
CviAII CATG 8 cut(s) 236, 250, 274, 557, 688, 1051, 1247, 1308
CviQI GTAC 4 cut(s) 727, 849, 1159, 1279
DdeI CTNAG 3 cut(s) 140, 470, 784
DpnI GATC 6 cut(s) 303, 516, 542, 561, 584, 1127
DpnII GATC 6 cut(s) 301, 514, 540, 559, 582, 1125
Eam1104I CTCTTC 3 cut(s) 555, 1070, 1149
EarI CTCTTC 3 cut(s) 555, 1070, 1149
Eco130I CCWWGG 1 cut(s) 1246
Eco147I AGGCCT 2 cut(s) 188, 701
Eco32I GATATC 2 cut(s) 1102, 1320
Eco47I GGWCC 2 cut(s) 215, 928
Eco57I CTGAAG 2 cut(s) 536, 864
Eco81I CCTNAGG 1 cut(s) 470
EcoRII CCWGG 2 cut(s) 405, 533
EcoRV GATATC 2 cut(s) 1102, 1320
EcoT14I CCWWGG 1 cut(s) 1246
EcoT22I ATGCAT 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 1246
FaeI CATG 8 cut(s) 239, 253, 277, 560, 691, 1054, 1250, 1311
FaqI GGGAC 2 cut(s) 423, 731
FatI CATG 8 cut(s) 235, 249, 273, 556, 687, 1050, 1246, 1307
FauI CCCGC 1 cut(s) 1101
FblI GTMKAC 1 cut(s) 1180
Fnu4HI GCNGC 5 cut(s) 246, 546, 917, 1039, 1332
FokI GGATG 7 cut(s) 131, 147, 194, 387, 403, 459, 550
Fsp4HI GCNGC 5 cut(s) 246, 546, 917, 1039, 1332
FspBI CTAG 4 cut(s) 227, 317, 563, 660
GluI GCNGC 5 cut(s) 246, 546, 917, 1039, 1332
HaeIII GGCC 3 cut(s) 188, 617, 701
Hin1II CATG 8 cut(s) 239, 253, 277, 560, 691, 1054, 1250, 1311
HincII GTYRAC 1 cut(s) 644
HindII GTYRAC 1 cut(s) 644
HinfI GANTC 5 cut(s) 359, 864, 900, 992, 1078
HphI GGTGA 1 cut(s) 1229
Hpy166II GTNNAC 3 cut(s) 336, 644, 1181
Hpy188I TCNGA 5 cut(s) 141, 514, 883, 892, 912
Hpy188III TCNNGA 8 cut(s) 89, 132, 317, 458, 599, 825, 1000, 1072
Hpy8I GTNNAC 3 cut(s) 336, 644, 1181
Hpy99I CGWCG 1 cut(s) 174
HpyAV CCTTC 2 cut(s) 172, 1195
HpyCH4III ACNGT 3 cut(s) 154, 197, 1378
HpyCH4IV ACGT 1 cut(s) 604
HpyF10VI GCNNNNNNNGC 2 cut(s) 665, 1035
HpyF3I CTNAG 3 cut(s) 140, 470, 784
HpySE526I ACGT 1 cut(s) 604
Hsp92II CATG 8 cut(s) 239, 253, 277, 560, 691, 1054, 1250, 1311
Kzo9I GATC 6 cut(s) 301, 514, 540, 559, 582, 1125
LguI GCTCTTC 1 cut(s) 555
LmnI GCTCC 1 cut(s) 418
Lsp1109I GCAGC 4 cut(s) 257, 928, 1050, 1343
LweI GCATC 7 cut(s) 94, 153, 232, 286, 365, 437, 1054
MaeI CTAG 4 cut(s) 227, 317, 563, 660
MaeII ACGT 1 cut(s) 604
MaeIII GTNAC 2 cut(s) 776, 1226
MalI GATC 6 cut(s) 303, 516, 542, 561, 584, 1127
MboI GATC 6 cut(s) 301, 514, 540, 559, 582, 1125
MboII GAAGA 8 cut(s) 71, 85, 311, 542, 722, 1087, 1163, 1166
MfeI CAATTG 2 cut(s) 210, 1138
MflI RGATCY 1 cut(s) 582
MhlI GDGCHC 1 cut(s) 338
MlyI GAGTC 2 cut(s) 353, 1087
MmeI TCCRAC 2 cut(s) 182, 305
Mph1103I ATGCAT 1 cut(s) 279
MroXI GAANNNNTTC 1 cut(s) 1205
MseI TTAA 4 cut(s) 437, 621, 966, 1113
MspCI CTTAAG 1 cut(s) 1112
MspR9I CCNGG 2 cut(s) 407, 535
MunI CAATTG 2 cut(s) 210, 1138
Mva1269I GAATGC 1 cut(s) 1243
MvaI CCWGG 2 cut(s) 407, 535
MvnI CGCG 2 cut(s) 545, 1096
MwoI GCNNNNNNNGC 2 cut(s) 665, 1035
NcoI CCATGG 1 cut(s) 1246
NdeII GATC 6 cut(s) 301, 514, 540, 559, 582, 1125
NheI GCTAGC 1 cut(s) 659
NlaIII CATG 8 cut(s) 239, 253, 277, 560, 691, 1054, 1250, 1311
NlaIV GGNNCC 1 cut(s) 156
NmuCI GTSAC 2 cut(s) 776, 1226
NsiI ATGCAT 1 cut(s) 279
NspI RCATGY 1 cut(s) 277
PceI AGGCCT 2 cut(s) 188, 701
PciSI GCTCTTC 1 cut(s) 555
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 1093
PctI GAATGC 1 cut(s) 1243
PdmI GAANNNNTTC 1 cut(s) 1205
PfeI GAWTC 3 cut(s) 864, 900, 992
PflMI CCANNNNNTGG 1 cut(s) 1308
PkrI GCNGC 5 cut(s) 247, 547, 918, 1040, 1333
PleI GAGTC 2 cut(s) 353, 1086
PpsI GAGTC 2 cut(s) 353, 1086
PshBI ATTAAT 1 cut(s) 437
Psp1406I AACGTT 1 cut(s) 604
Psp6I CCWGG 2 cut(s) 405, 533
PspGI CCWGG 2 cut(s) 405, 533
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 2 cut(s) 215, 928
PsuI RGATCY 1 cut(s) 582
RsaI GTAC 4 cut(s) 728, 850, 1160, 1280
RsaNI GTAC 4 cut(s) 727, 849, 1159, 1279
SapI GCTCTTC 1 cut(s) 555
SaqAI TTAA 4 cut(s) 437, 621, 966, 1113
SatI GCNGC 5 cut(s) 246, 546, 917, 1039, 1332
Sau3AI GATC 6 cut(s) 301, 514, 540, 559, 582, 1125
Sau96I GGNCC 2 cut(s) 215, 928
ScaI AGTACT 2 cut(s) 850, 1160
SchI GAGTC 2 cut(s) 353, 1087
ScrFI CCNGG 2 cut(s) 407, 535
SduI GDGCHC 1 cut(s) 338
SfaNI GCATC 7 cut(s) 94, 153, 232, 286, 365, 437, 1054
SinI GGWCC 2 cut(s) 215, 928
SmlI CTYRAG 2 cut(s) 1000, 1112
SmoI CTYRAG 2 cut(s) 1000, 1112
SseBI AGGCCT 2 cut(s) 188, 701
SsiI CCGC 3 cut(s) 112, 545, 1094
SspI AATATT 1 cut(s) 522
SspMI CTAG 4 cut(s) 227, 317, 563, 660
StuI AGGCCT 2 cut(s) 188, 701
StyD4I CCNGG 2 cut(s) 405, 533
StyI CCWWGG 1 cut(s) 1246
TaaI ACNGT 3 cut(s) 154, 197, 1378
TaiI ACGT 1 cut(s) 607
TaqI TCGA 5 cut(s) 169, 573, 600, 903, 1020
TatI WGTACW 4 cut(s) 726, 848, 1158, 1278
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 3 cut(s) 864, 900, 992
Tru1I TTAA 4 cut(s) 437, 621, 966, 1113
Tru9I TTAA 4 cut(s) 437, 621, 966, 1113
TscAI CASTG 1 cut(s) 504
TseFI GTSAC 2 cut(s) 776, 1226
TseI GCWGC 4 cut(s) 245, 916, 1038, 1331
Tsp45I GTSAC 2 cut(s) 776, 1226
TspDTI ATGAA 5 cut(s) 442, 827, 973, 1203, 1336
TspGWI ACGGA 2 cut(s) 459, 941
TspRI CASTG 1 cut(s) 504
Van91I CCANNNNNTGG 1 cut(s) 1308
Vha464I CTTAAG 1 cut(s) 1112
VneI GTGCAC 1 cut(s) 334
VpaK11BI GGWCC 2 cut(s) 215, 928
VspI ATTAAT 1 cut(s) 437
XapI RAATTY 4 cut(s) 41, 366, 755, 969
XbaI TCTAGA 1 cut(s) 316
XceI RCATGY 1 cut(s) 277
XmiI GTMKAC 1 cut(s) 1180
XmnI GAANNNNTTC 1 cut(s) 1205
XspI CTAG 4 cut(s) 227, 317, 563, 660
ZrmI AGTACT 2 cut(s) 850, 1160
Zsp2I ATGCAT 1 cut(s) 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.