Rh3DG128300

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0016364
Physical Location & Seq
Reverse (-)
10898975 .. 10902853
3879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG128300.1

Sequence Viewer

Length: 1374 bp
ATGACTGAATTCCAGCAGGAAATCCAGCAAGTAGAAGCAATTGACAAGAAGAAAGAGTGGCATCGGGAGATTACTGAAAAGCTTCACCGCAAGGTGGAAAGTGGTTCTGGATGTCTGAGCATCCACACGGTTCCAACGAGGCTTCGACGAGTGAAGGATGAGGCCTACAACTGTCGCATTGTCTCAATTGGTCCTTTGCATCTAGGCAACCATGACCTTGCAGCCATGAGAGAGCGTAAATGGGCTTACATGAATCACTTATTTCAGCGAATTGAAGATCGTCAGCAGTTTCTAGAGTGTTGGACAGAGTGCACGAGTGCTGTTTATGATTTAGACTCACAAATTCGTGGATGCTACGCTAAAAGGATGAGCCACAAAGACAAGGGACACGAGGAGCTTGCAGAAGTTGTATTAATTGACGGATGCTTCATTCTGGAACTTTTCCTCAAGTATGAGGAACACTTGAATATGACACTGGATTATCAATCTGATCCAATATTCAATAGTGCCTGGATGATCGCGGCTCTTCAGCATGATCTAGCACTACTCGAAAACCAGATCCCCTTTATGATTCTCGAACGTTTATATGAGGCCATTAAGCCTCACCTAAAGAATAGTCGACCTCGACCAGACTCTGTCGCTAGCCTTGCTCTTACTTTTTTTCAACCCATGAACTATAAGGCCTTTTTAGAAGATTGGGACACTCAGTACAACCATTTGCTTGATGTCTTGCACAAATTTTACCTCCCAAGTAATTGTGACATCTCAGTAGAAATTGAAATGATTACCGAAATGAAACATCATTCCAGACCTGAACCAGTATTCGGCAGTACTAAAAAATGGGGATTCAATTTTTGTGCTTCAGAGCTTTCGGAGGCTGGAATCGAGTTTCAGAAGCAGCAGAGGACGGACCATTTGTTGAACATAACTTTCAGCAGGGGAGTAATTAAAATTCCACCGATTTTCATTGACGATTCACTCTTGAGGAACTTGATTGCTTTCGAGCAATGCAGTCTTGGCAGCCTGCATCACATGACATCATACGCAATTCTCTTGAAGAGTCTCATTCGTTCCCCGCGAGATATCAGACTACTTATGCAAAGAGGGATCATAAGTTCCGATTGGGTTAGAGAAGAAGAGTACTTGTCTCAACTCAAAAGTATACTTGATGAAGTTGTTGTGAAGGAGTTCTACTTTGGTGATTTGTGTGACAAAGTGAATGCTTACCATGGCAAGTTCTGGTTTCGCAGAGGTATAAAGTACATTTATCGCACTTATTTCTCCACAGCATGGAGTATGATATCATTTGTTGCAGCCATTGTACTTTTCATTCTCACAGCTTTACAAACATTCTATACTGTCCGCCCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

457

Amino Acids

53.77

Weight (kDa)

6.56

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 41 - 445 4.3e-97 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1290
AccI GTMKAC 2 cut(s) 619, 1162
AccII CGCG 2 cut(s) 521, 1078
AciI CCGC 4 cut(s) 88, 521, 1076, 1363
AclI AACGTT 1 cut(s) 580
AclWI GGATC 3 cut(s) 485, 553, 1115
AcsI RAATTY 4 cut(s) 8, 342, 737, 951
AcuI CTGAAG 2 cut(s) 512, 846
AfaI GTAC 5 cut(s) 710, 832, 1142, 1262, 1323
AfiI CCNNNNNNNGG 3 cut(s) 94, 824, 1290
AgsI TTSAA 8 cut(s) 275, 466, 502, 665, 779, 850, 922, 1057
AjnI CCWGG 1 cut(s) 509
AloI GAACNNNNNNTCC 2 cut(s) 1099, 1131
AluBI AGCT 4 cut(s) 82, 397, 868, 1340
AluI AGCT 4 cut(s) 82, 397, 868, 1340
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 3 cut(s) 187, 1067, 1152
Alw44I GTGCAC 1 cut(s) 310
AlwI GGATC 3 cut(s) 485, 553, 1115
AlwNI CAGNNNCTG 1 cut(s) 635
AoxI GGCC 3 cut(s) 162, 591, 681
ApaLI GTGCAC 1 cut(s) 310
ApeKI GCWGC 4 cut(s) 221, 898, 1020, 1313
ApoI RAATTY 4 cut(s) 8, 342, 737, 951
ArsI GACNNNNNNTTYG 2 cut(s) 898, 930
AseI ATTAAT 1 cut(s) 413
Asp700I GAANNNNTTC 2 cut(s) 81, 1187
AspS9I GGNCC 2 cut(s) 191, 910
AsuHPI GGTGA 3 cut(s) 77, 596, 1211
AsuNHI GCTAGC 1 cut(s) 641
AvaII GGWCC 2 cut(s) 191, 910
BaeGI GKGCMC 1 cut(s) 314
BarI GAAGNNNNNNTAC 2 cut(s) 1175, 1207
BauI CACGAG 2 cut(s) 313, 389
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 4 cut(s) 233, 910, 1032, 1325
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BciT130I CCWGG 1 cut(s) 511
BcoDI GTCTC 3 cut(s) 187, 1067, 1152
BfaI CTAG 4 cut(s) 203, 293, 539, 642
BisI GCNGC 5 cut(s) 222, 522, 899, 1021, 1314
BlsI GCNGC 5 cut(s) 223, 523, 900, 1022, 1315
BmcAI AGTACT 2 cut(s) 832, 1142
Bme1390I CCNGG 1 cut(s) 511
Bme18I GGWCC 2 cut(s) 191, 910
BmgT120I GGNCC 2 cut(s) 191, 910
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 511
BmsI GCATC 6 cut(s) 70, 129, 208, 341, 413, 1036
BmtI GCTAGC 1 cut(s) 645
BpuEI CTTGAG 2 cut(s) 431, 1003
BsaJI CCNNGG 1 cut(s) 1228
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 824, 1290
Bse1I ACTGG 2 cut(s) 480, 818
Bse3DI GCAATG 1 cut(s) 1013
BseBI CCWGG 1 cut(s) 511
BseDI CCNNGG 1 cut(s) 1228
BseGI GGATG 7 cut(s) 116, 120, 163, 356, 372, 428, 519
BseLI CCNNNNNNNGG 3 cut(s) 94, 824, 1290
BseMI GCAATG 1 cut(s) 1013
BseMII CTCAG 3 cut(s) 107, 719, 780
BseNI ACTGG 2 cut(s) 480, 818
BseRI GAGGAG 1 cut(s) 407
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 4 cut(s) 233, 910, 1032, 1325
Bsh1236I CGCG 2 cut(s) 521, 1078
BshFI GGCC 3 cut(s) 164, 593, 683
BsiHKAI GWGCWC 1 cut(s) 314
BslFI GGGAC 2 cut(s) 399, 713
BslI CCNNNNNNNGG 3 cut(s) 94, 824, 1290
BsmAI GTCTC 3 cut(s) 187, 1067, 1152
BsmFI GGGAC 2 cut(s) 399, 713
BsmI GAATGC 1 cut(s) 1225
BsnI GGCC 3 cut(s) 164, 593, 683
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 6 cut(s) 277, 490, 516, 535, 558, 1107
Bsp19I CCATGG 1 cut(s) 1228
BspACI CCGC 4 cut(s) 88, 521, 1076, 1363
BspANI GGCC 3 cut(s) 164, 593, 683
BspCNI CTCAG 3 cut(s) 108, 718, 779
BspFNI CGCG 2 cut(s) 521, 1078
BspLI GGNNCC 1 cut(s) 132
BspOI GCTAGC 1 cut(s) 645
BspPI GGATC 3 cut(s) 485, 553, 1115
BspQI GCTCTTC 1 cut(s) 531
BsrDI GCAATG 1 cut(s) 1013
BsrI ACTGG 2 cut(s) 480, 818
BssECI CCNNGG 1 cut(s) 1228
BssMI GATC 6 cut(s) 277, 490, 516, 535, 558, 1107
BssNAI GTATAC 1 cut(s) 1163
BssSI CACGAG 2 cut(s) 313, 389
BssT1I CCWWGG 1 cut(s) 1228
Bst1107I GTATAC 1 cut(s) 1163
Bst2BI CACGAG 2 cut(s) 313, 389
Bst2UI CCWGG 1 cut(s) 511
Bst4CI ACNGT 3 cut(s) 130, 173, 1360
Bst6I CTCTTC 3 cut(s) 531, 1052, 1131
BstC8I GCNNGC 3 cut(s) 399, 643, 1025
BstDEI CTNAG 3 cut(s) 116, 705, 766
BstDSI CCRYGG 1 cut(s) 1228
BstF5I GGATG 7 cut(s) 116, 120, 163, 356, 372, 428, 519
BstFNI CGCG 2 cut(s) 521, 1078
BstKTI GATC 6 cut(s) 280, 493, 519, 538, 561, 1110
BstMAI GTCTC 3 cut(s) 187, 1067, 1152
BstMBI GATC 6 cut(s) 277, 490, 516, 535, 558, 1107
BstMWI GCNNNNNNNGC 2 cut(s) 647, 1017
BstNI CCWGG 1 cut(s) 511
BstSCI CCNGG 1 cut(s) 509
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 2 cut(s) 521, 1078
BstV1I GCAGC 4 cut(s) 233, 910, 1032, 1325
BstX2I RGATCY 1 cut(s) 558
BstYI RGATCY 1 cut(s) 558
BstZ17I GTATAC 1 cut(s) 1163
BsuRI GGCC 3 cut(s) 164, 593, 683
BtgI CCRYGG 1 cut(s) 1228
BtsCI GGATG 7 cut(s) 116, 120, 163, 356, 372, 428, 519
BtsIMutI CAGTG 1 cut(s) 473
Cac8I GCNNGC 3 cut(s) 399, 643, 1025
CaiI CAGNNNCTG 1 cut(s) 635
Cfr13I GGNCC 2 cut(s) 191, 910
Csp6I GTAC 5 cut(s) 709, 831, 1141, 1261, 1322
CviAII CATG 8 cut(s) 212, 226, 250, 533, 670, 1033, 1229, 1290
CviQI GTAC 5 cut(s) 709, 831, 1141, 1261, 1322
DdeI CTNAG 3 cut(s) 116, 705, 766
DpnI GATC 6 cut(s) 279, 492, 518, 537, 560, 1109
DpnII GATC 6 cut(s) 277, 490, 516, 535, 558, 1107
Eam1104I CTCTTC 3 cut(s) 531, 1052, 1131
EarI CTCTTC 3 cut(s) 531, 1052, 1131
EciI GGCGGA 1 cut(s) 1352
Eco130I CCWWGG 1 cut(s) 1228
Eco147I AGGCCT 2 cut(s) 164, 683
Eco32I GATATC 2 cut(s) 1084, 1302
Eco47I GGWCC 2 cut(s) 191, 910
Eco57I CTGAAG 2 cut(s) 512, 846
EcoRI GAATTC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 509
EcoRV GATATC 2 cut(s) 1084, 1302
EcoT14I CCWWGG 1 cut(s) 1228
ErhI CCWWGG 1 cut(s) 1228
FaeI CATG 8 cut(s) 215, 229, 253, 536, 673, 1036, 1232, 1293
FaqI GGGAC 2 cut(s) 399, 713
FatI CATG 8 cut(s) 211, 225, 249, 532, 669, 1032, 1228, 1289
FauI CCCGC 1 cut(s) 1083
FblI GTMKAC 2 cut(s) 619, 1162
Fnu4HI GCNGC 5 cut(s) 222, 522, 899, 1021, 1314
FokI GGATG 7 cut(s) 107, 123, 170, 363, 379, 435, 526
Fsp4HI GCNGC 5 cut(s) 222, 522, 899, 1021, 1314
FspBI CTAG 4 cut(s) 203, 293, 539, 642
GluI GCNGC 5 cut(s) 222, 522, 899, 1021, 1314
HaeIII GGCC 3 cut(s) 164, 593, 683
Hin1II CATG 8 cut(s) 215, 229, 253, 536, 673, 1036, 1232, 1293
HincII GTYRAC 1 cut(s) 620
HindII GTYRAC 1 cut(s) 620
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 8 cut(s) 253, 335, 571, 632, 846, 882, 974, 1060
HphI GGTGA 3 cut(s) 77, 596, 1211
Hpy166II GTNNAC 3 cut(s) 312, 620, 1163
Hpy188I TCNGA 7 cut(s) 117, 490, 865, 874, 894, 1088, 1120
Hpy188III TCNNGA 8 cut(s) 65, 108, 293, 434, 575, 807, 982, 1054
Hpy8I GTNNAC 3 cut(s) 312, 620, 1163
Hpy99I CGWCG 1 cut(s) 150
HpyAV CCTTC 2 cut(s) 148, 1177
HpyCH4III ACNGT 3 cut(s) 130, 173, 1360
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 9 cut(s) 199, 221, 312, 401, 733, 1011, 1027, 1099, 1313
HpyF10VI GCNNNNNNNGC 2 cut(s) 647, 1017
HpyF3I CTNAG 3 cut(s) 116, 705, 766
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 8 cut(s) 215, 229, 253, 536, 673, 1036, 1232, 1293
Kzo9I GATC 6 cut(s) 277, 490, 516, 535, 558, 1107
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 1 cut(s) 394
Lsp1109I GCAGC 4 cut(s) 233, 910, 1032, 1325
LweI GCATC 6 cut(s) 70, 129, 208, 341, 413, 1036
MaeI CTAG 4 cut(s) 203, 293, 539, 642
MaeII ACGT 1 cut(s) 580
MaeIII GTNAC 2 cut(s) 758, 1208
MalI GATC 6 cut(s) 279, 492, 518, 537, 560, 1109
MboI GATC 6 cut(s) 277, 490, 516, 535, 558, 1107
MboII GAAGA 7 cut(s) 61, 287, 518, 704, 1069, 1145, 1148
MfeI CAATTG 2 cut(s) 39, 186
MflI RGATCY 1 cut(s) 558
MhlI GDGCHC 1 cut(s) 314
MlyI GAGTC 3 cut(s) 329, 626, 1069
MmeI TCCRAC 2 cut(s) 158, 281
MroXI GAANNNNTTC 2 cut(s) 81, 1187
MseI TTAA 3 cut(s) 413, 597, 948
MspR9I CCNGG 1 cut(s) 511
MunI CAATTG 2 cut(s) 39, 186
Mva1269I GAATGC 1 cut(s) 1225
MvaI CCWGG 1 cut(s) 511
MvnI CGCG 2 cut(s) 521, 1078
MwoI GCNNNNNNNGC 2 cut(s) 647, 1017
NcoI CCATGG 1 cut(s) 1228
NdeII GATC 6 cut(s) 277, 490, 516, 535, 558, 1107
NheI GCTAGC 1 cut(s) 641
NlaIII CATG 8 cut(s) 215, 229, 253, 536, 673, 1036, 1232, 1293
NlaIV GGNNCC 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 758, 1208
PceI AGGCCT 2 cut(s) 164, 683
PciSI GCTCTTC 1 cut(s) 531
PcsI WCGNNNNNNNCGW 2 cut(s) 134, 1075
PctI GAATGC 1 cut(s) 1225
PdmI GAANNNNTTC 2 cut(s) 81, 1187
PfeI GAWTC 5 cut(s) 253, 571, 846, 882, 974
PflFI GACNNNGTC 1 cut(s) 635
PflMI CCANNNNNTGG 1 cut(s) 1290
PkrI GCNGC 5 cut(s) 223, 523, 900, 1022, 1315
PleI GAGTC 3 cut(s) 329, 626, 1068
PpsI GAGTC 3 cut(s) 329, 626, 1068
PshBI ATTAAT 1 cut(s) 413
Psp1406I AACGTT 1 cut(s) 580
Psp6I CCWGG 1 cut(s) 509
PspGI CCWGG 1 cut(s) 509
PspN4I GGNNCC 1 cut(s) 132
PspPI GGNCC 2 cut(s) 191, 910
PstNI CAGNNNCTG 1 cut(s) 635
PsuI RGATCY 1 cut(s) 558
PsyI GACNNNGTC 1 cut(s) 635
RsaI GTAC 5 cut(s) 710, 832, 1142, 1262, 1323
RsaNI GTAC 5 cut(s) 709, 831, 1141, 1261, 1322
SalI GTCGAC 1 cut(s) 618
SapI GCTCTTC 1 cut(s) 531
SaqAI TTAA 3 cut(s) 413, 597, 948
SatI GCNGC 5 cut(s) 222, 522, 899, 1021, 1314
Sau3AI GATC 6 cut(s) 277, 490, 516, 535, 558, 1107
Sau96I GGNCC 2 cut(s) 191, 910
ScaI AGTACT 2 cut(s) 832, 1142
SchI GAGTC 3 cut(s) 329, 626, 1069
ScrFI CCNGG 1 cut(s) 511
SduI GDGCHC 1 cut(s) 314
SfaNI GCATC 6 cut(s) 70, 129, 208, 341, 413, 1036
SinI GGWCC 2 cut(s) 191, 910
SmlI CTYRAG 2 cut(s) 446, 982
SmoI CTYRAG 2 cut(s) 446, 982
SseBI AGGCCT 2 cut(s) 164, 683
SsiI CCGC 4 cut(s) 88, 521, 1076, 1363
SspI AATATT 1 cut(s) 498
SspMI CTAG 4 cut(s) 203, 293, 539, 642
StuI AGGCCT 2 cut(s) 164, 683
StyD4I CCNGG 1 cut(s) 509
StyI CCWWGG 1 cut(s) 1228
TaaI ACNGT 3 cut(s) 130, 173, 1360
TaiI ACGT 1 cut(s) 583
TaqI TCGA 7 cut(s) 145, 549, 576, 619, 625, 885, 1002
TatI WGTACW 5 cut(s) 708, 830, 1140, 1260, 1321
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 5 cut(s) 253, 571, 846, 882, 974
Tru1I TTAA 3 cut(s) 413, 597, 948
Tru9I TTAA 3 cut(s) 413, 597, 948
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 2 cut(s) 758, 1208
TseI GCWGC 4 cut(s) 221, 898, 1020, 1313
Tsp45I GTSAC 2 cut(s) 758, 1208
TspDTI ATGAA 7 cut(s) 266, 418, 686, 809, 955, 1185, 1318
TspGWI ACGGA 2 cut(s) 435, 923
TspRI CASTG 1 cut(s) 480
Tth111I GACNNNGTC 1 cut(s) 635
Van91I CCANNNNNTGG 1 cut(s) 1290
VneI GTGCAC 1 cut(s) 310
VpaK11BI GGWCC 2 cut(s) 191, 910
VspI ATTAAT 1 cut(s) 413
XapI RAATTY 4 cut(s) 8, 342, 737, 951
XbaI TCTAGA 1 cut(s) 292
XmiI GTMKAC 2 cut(s) 619, 1162
XmnI GAANNNNTTC 2 cut(s) 81, 1187
XspI CTAG 4 cut(s) 203, 293, 539, 642
ZrmI AGTACT 2 cut(s) 832, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.