RLG00000027234

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012653
Physical Location & Seq
Reverse (-)
9794679 .. 9795337
659 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027234

Sequence Viewer

Length: 552 bp
ATGGAGAAGAACCAGAATGGCGTCGTTGGGGAGCATGACACGTCGAAACCTCCCAAGTTTAGCATCATTCGATTTACGTATATGGTGGATCCCTATAATAGTTGCATCACATTGTCAACAACCAGGAAGAAACCAGCTGTTATTCGCAGAGCCAAGGAGTTTCTTCAACTTGTAGCGGCACACATGCCCGTACATGTAGCATTTACACTCTTTACTGGACGGCTATCATCTTATATCATCAAGATCTGGCGTCAAGATGGTGGCCTTTGCAGAGACATAGGGGTCAGCGGGGAACAATTTCATGAACGGAGGAATCTCTTCCGGGACTCCGTAGACGCACTTGAAAGGTTGACGTCCTGCACAATTATCATACAGTCTATGACTATCGTGGTTATCGGTGAATCCGAAGGAATAAAGATAGTCAGGGATATGGTGAAAGACTGCTTTGTTTCCGATGAGTCTCCTGCACCAAAGATCAGGAGAGCCGAGGGGGAACTGGCGAGAAAGGATATCGAGGCTGTGCGCCGCCGACTTCATGAGAAGCAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.93

Weight (kDa)

9.41

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_2nd PF21800 99 - 161 9.8e-10 KRR1 small subunit processome component, second KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
AatII GACGTC 1 cut(s) 356
AccI GTMKAC 1 cut(s) 333
AciI CCGC 3 cut(s) 176, 288, 526
AclWI GGATC 2 cut(s) 83, 96
AcyI GRCGYC 3 cut(s) 21, 250, 353
AfaI GTAC 1 cut(s) 192
AflIII ACRYGT 2 cut(s) 39, 193
AgsI TTSAA 2 cut(s) 167, 344
AjiI CACGTC 1 cut(s) 42
AjnI CCWGG 1 cut(s) 122
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw26I GTCTC 2 cut(s) 267, 465
AlwI GGATC 2 cut(s) 83, 96
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 1 cut(s) 544
Asp700I GAANNNNTTC 2 cut(s) 297, 317
AspLEI GCGC 1 cut(s) 525
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 2 cut(s) 410, 445
BamHI GGATCC 1 cut(s) 88
BccI CCATC 1 cut(s) 251
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 124
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 2 cut(s) 267, 465
BglII AGATCT 1 cut(s) 243
BisI GCNGC 3 cut(s) 177, 526, 545
BlsI GCNGC 3 cut(s) 178, 527, 546
Bme1390I CCNGG 2 cut(s) 124, 323
BmgBI CACGTC 1 cut(s) 42
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 2 cut(s) 124, 323
BmsI GCATC 2 cut(s) 72, 114
BpuMI CCSGG 1 cut(s) 323
BsaAI YACGTR 1 cut(s) 78
BsaHI GRCGYC 3 cut(s) 21, 250, 353
BsaJI CCNNGG 2 cut(s) 153, 486
Bse1I ACTGG 2 cut(s) 220, 501
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 153, 486
BseNI ACTGG 2 cut(s) 220, 501
BsgI GTGCAG 2 cut(s) 343, 450
BshFI GGCC 1 cut(s) 264
BsiSI CCGG 1 cut(s) 322
BslFI GGGAC 1 cut(s) 338
BsmAI GTCTC 2 cut(s) 267, 465
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 1 cut(s) 264
Bsp143I GATC 3 cut(s) 88, 243, 474
BspACI CCGC 3 cut(s) 176, 288, 526
BspANI GGCC 1 cut(s) 264
BspHI TCATGA 2 cut(s) 301, 535
BspLI GGNNCC 1 cut(s) 90
BspPI GGATC 2 cut(s) 83, 96
BsrI ACTGG 2 cut(s) 220, 501
BssECI CCNNGG 2 cut(s) 153, 486
BssMI GATC 3 cut(s) 88, 243, 474
BssNI GRCGYC 3 cut(s) 21, 250, 353
BssT1I CCWWGG 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 1 cut(s) 375
Bst6I CTCTTC 1 cut(s) 323
BstACI GRCGYC 3 cut(s) 21, 250, 353
BstBAI YACGTR 1 cut(s) 78
BstHHI GCGC 1 cut(s) 525
BstKTI GATC 3 cut(s) 91, 246, 477
BstMAI GTCTC 2 cut(s) 267, 465
BstMBI GATC 3 cut(s) 88, 243, 474
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 2 cut(s) 187, 197
BstSCI CCNGG 2 cut(s) 122, 321
BstSNI TACGTA 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 88, 243
BstYI RGATCY 2 cut(s) 88, 243
BsuRI GGCC 1 cut(s) 264
BtrI CACGTC 1 cut(s) 42
CciI TCATGA 2 cut(s) 301, 535
CfoI GCGC 1 cut(s) 525
CseI GACGC 3 cut(s) 10, 239, 344
Csp6I GTAC 1 cut(s) 191
CviAII CATG 5 cut(s) 35, 184, 194, 302, 536
CviJI RGCY 6 cut(s) 137, 152, 223, 264, 485, 518
CviKI_1 RGCY 6 cut(s) 137, 152, 223, 264, 485, 518
CviQI GTAC 1 cut(s) 191
DpnI GATC 3 cut(s) 90, 245, 476
DpnII GATC 3 cut(s) 88, 243, 474
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
Eam1104I CTCTTC 1 cut(s) 323
EarI CTCTTC 1 cut(s) 323
Eco105I TACGTA 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 153
Eco32I GATATC 1 cut(s) 511
EcoRII CCWGG 1 cut(s) 122
EcoRV GATATC 1 cut(s) 511
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 5 cut(s) 38, 187, 197, 305, 539
FaqI GGGAC 1 cut(s) 338
FatI CATG 5 cut(s) 34, 183, 193, 301, 535
FauI CCCGC 1 cut(s) 281
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 3 cut(s) 177, 526, 545
Fsp4HI GCNGC 3 cut(s) 177, 526, 545
GlaI GCGC 1 cut(s) 524
GluI GCNGC 3 cut(s) 177, 526, 545
HaeIII GGCC 1 cut(s) 264
HapII CCGG 1 cut(s) 322
HgaI GACGC 3 cut(s) 10, 239, 344
HhaI GCGC 1 cut(s) 525
Hin1I GRCGYC 3 cut(s) 21, 250, 353
Hin1II CATG 5 cut(s) 38, 187, 197, 305, 539
Hin6I GCGC 1 cut(s) 523
HinP1I GCGC 1 cut(s) 523
HincII GTYRAC 2 cut(s) 117, 351
HindII GTYRAC 2 cut(s) 117, 351
HinfI GANTC 4 cut(s) 313, 326, 401, 458
HpaII CCGG 1 cut(s) 322
HphI GGTGA 2 cut(s) 410, 445
Hpy166II GTNNAC 3 cut(s) 117, 334, 351
Hpy188I TCNGA 2 cut(s) 406, 454
Hpy188III TCNNGA 5 cut(s) 241, 254, 302, 478, 536
Hpy8I GTNNAC 3 cut(s) 117, 334, 351
Hpy99I CGWCG 2 cut(s) 26, 46
HpyAV CCTTC 1 cut(s) 401
HpyCH4III ACNGT 1 cut(s) 375
HpyCH4IV ACGT 3 cut(s) 41, 77, 353
HpyCH4V TGCA 4 cut(s) 105, 270, 360, 467
HpySE526I ACGT 3 cut(s) 41, 77, 353
Hsp92I GRCGYC 3 cut(s) 21, 250, 353
Hsp92II CATG 5 cut(s) 38, 187, 197, 305, 539
HspAI GCGC 1 cut(s) 523
Kzo9I GATC 3 cut(s) 88, 243, 474
LmnI GCTCC 1 cut(s) 31
LweI GCATC 2 cut(s) 72, 114
MaeII ACGT 3 cut(s) 41, 77, 353
MalI GATC 3 cut(s) 90, 245, 476
MboI GATC 3 cut(s) 88, 243, 474
MboII GAAGA 4 cut(s) 19, 139, 155, 310
MflI RGATCY 2 cut(s) 88, 243
MluCI AATT 2 cut(s) 296, 363
MlyI GAGTC 2 cut(s) 320, 467
MnlI CCTC 4 cut(s) 60, 303, 481, 508
MroXI GAANNNNTTC 2 cut(s) 297, 317
MspA1I CMGCKG 2 cut(s) 137, 288
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 2 cut(s) 124, 323
MvaI CCWGG 1 cut(s) 124
NciI CCSGG 1 cut(s) 323
NdeII GATC 3 cut(s) 88, 243, 474
NlaIII CATG 5 cut(s) 38, 187, 197, 305, 539
NlaIV GGNNCC 1 cut(s) 90
NmeAIII GCCGAG 1 cut(s) 511
NspI RCATGY 2 cut(s) 187, 197
PagI TCATGA 2 cut(s) 301, 535
PciI ACATGT 1 cut(s) 193
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PdmI GAANNNNTTC 2 cut(s) 297, 317
PfeI GAWTC 2 cut(s) 313, 401
PfoI TCCNGGA 1 cut(s) 321
PkrI GCNGC 3 cut(s) 178, 527, 546
PleI GAGTC 2 cut(s) 320, 466
PpsI GAGTC 2 cut(s) 320, 466
Ppu21I YACGTR 1 cut(s) 78
PscI ACATGT 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 1 cut(s) 90
PsuI RGATCY 2 cut(s) 88, 243
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
SatI GCNGC 3 cut(s) 177, 526, 545
Sau3AI GATC 3 cut(s) 88, 243, 474
SchI GAGTC 2 cut(s) 320, 467
ScrFI CCNGG 2 cut(s) 124, 323
SetI ASST 6 cut(s) 44, 52, 80, 139, 350, 356
SfaNI GCATC 2 cut(s) 72, 114
SnaBI TACGTA 1 cut(s) 78
Sse9I AATT 2 cut(s) 296, 363
SsiI CCGC 3 cut(s) 176, 288, 526
StyD4I CCNGG 2 cut(s) 122, 321
StyI CCWWGG 1 cut(s) 153
TaaI ACNGT 1 cut(s) 375
TaiI ACGT 3 cut(s) 44, 80, 356
TaqI TCGA 3 cut(s) 44, 70, 513
TasI AATT 2 cut(s) 296, 363
TauI GCSGC 2 cut(s) 179, 528
TfiI GAWTC 2 cut(s) 313, 401
TseI GCWGC 1 cut(s) 544
TspDTI ATGAA 3 cut(s) 290, 318, 524
TspGWI ACGGA 2 cut(s) 319, 322
XceI RCATGY 2 cut(s) 187, 197
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 2 cut(s) 297, 317
ZraI GACGTC 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.