RLG00000027235

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012653
Physical Location & Seq
Reverse (-)
9801021 .. 9801705
685 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027235

Sequence Viewer

Length: 603 bp
ATGAAGAAGAACCAGAATGGCGTCGTTGGGGAGCATGACACGTCGAAACCTCCCAAGGTCAATACGTTTTACCCTTCATGGAGCGACGAAGGGATTCTTCAACTTGAATTTAGCATCATTCGATTTACGTATATGGTGGATCCCTATAATAGTTGCATCACATTGTCAACAACCAGGAAGAAACCAGCTGTTATTCGCAGAGCCAAGGAGTTTCTTCAACTTGTAGCGGCACACATGCCCGTACATGTAGCATTTACACTCTTTACTGGACGGCTATCATTTTATATCATCAAGATCTGGCGTCAAGATGGTGGCCTTTGCAGAGACATAGGGGTCAGCGGGGAACAATTTCATGAACGGAGGAATCTTTTCCGGGACTCCATAGACGCACTTGAAAGGTTGACGTCCTGCAAAATTATCATACAGTCTACGACTATTGTGGTTATCGGTAAATCCGAAGGAATAAAGATAGTCAGGGATATGGTGAAAGACTGCTTTGTTTCCAATGAGTCTCCTGCACCAAAGATCAGGAGAGCCGAGAGGGAACTGGCGAGGAAGGATATCGAGGCTGTGCGCCGCCGACTTCATGAGAAGCAGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

23.07

Weight (kDa)

9.72

Isoelectric Point (pI)

49.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_2nd PF21800 116 - 181 4.8e-11 KRR1 small subunit processome component, second KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
AatII GACGTC 1 cut(s) 407
AccI GTMKAC 1 cut(s) 428
AciI CCGC 3 cut(s) 227, 339, 577
AclWI GGATC 2 cut(s) 134, 147
AcsI RAATTY 1 cut(s) 107
AcyI GRCGYC 3 cut(s) 21, 301, 404
AfaI GTAC 1 cut(s) 243
AflIII ACRYGT 2 cut(s) 39, 244
AgsI TTSAA 4 cut(s) 101, 107, 218, 395
AjiI CACGTC 1 cut(s) 42
AjnI CCWGG 1 cut(s) 173
AluBI AGCT 1 cut(s) 188
AluI AGCT 1 cut(s) 188
Alw26I GTCTC 2 cut(s) 318, 516
AlwI GGATC 2 cut(s) 134, 147
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 1 cut(s) 595
ApoI RAATTY 1 cut(s) 107
Asp700I GAANNNNTTC 3 cut(s) 93, 348, 368
AspLEI GCGC 1 cut(s) 576
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 1 cut(s) 496
BamHI GGATCC 1 cut(s) 139
BccI CCATC 1 cut(s) 302
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 1 cut(s) 175
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 2 cut(s) 318, 516
BglII AGATCT 1 cut(s) 294
BisI GCNGC 3 cut(s) 228, 577, 596
BlsI GCNGC 3 cut(s) 229, 578, 597
Bme1390I CCNGG 2 cut(s) 175, 374
BmgBI CACGTC 1 cut(s) 42
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 2 cut(s) 175, 374
BmsI GCATC 2 cut(s) 123, 165
BpuMI CCSGG 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 129
BsaHI GRCGYC 3 cut(s) 21, 301, 404
BsaJI CCNNGG 2 cut(s) 54, 204
Bse1I ACTGG 2 cut(s) 271, 552
BseBI CCWGG 1 cut(s) 175
BseDI CCNNGG 2 cut(s) 54, 204
BseNI ACTGG 2 cut(s) 271, 552
BsgI GTGCAG 1 cut(s) 501
BshFI GGCC 1 cut(s) 315
BsiSI CCGG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 389
BsmAI GTCTC 2 cut(s) 318, 516
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 1 cut(s) 315
Bsp143I GATC 3 cut(s) 139, 294, 525
BspACI CCGC 3 cut(s) 227, 339, 577
BspANI GGCC 1 cut(s) 315
BspHI TCATGA 2 cut(s) 352, 586
BspLI GGNNCC 1 cut(s) 141
BspPI GGATC 2 cut(s) 134, 147
BsrI ACTGG 2 cut(s) 271, 552
BssECI CCNNGG 2 cut(s) 54, 204
BssMI GATC 3 cut(s) 139, 294, 525
BssNI GRCGYC 3 cut(s) 21, 301, 404
BssT1I CCWWGG 2 cut(s) 54, 204
Bst2UI CCWGG 1 cut(s) 175
Bst4CI ACNGT 1 cut(s) 426
BstACI GRCGYC 3 cut(s) 21, 301, 404
BstBAI YACGTR 1 cut(s) 129
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 3 cut(s) 142, 297, 528
BstMAI GTCTC 2 cut(s) 318, 516
BstMBI GATC 3 cut(s) 139, 294, 525
BstNI CCWGG 1 cut(s) 175
BstNSI RCATGY 2 cut(s) 238, 248
BstSCI CCNGG 2 cut(s) 173, 372
BstSNI TACGTA 1 cut(s) 129
BstX2I RGATCY 2 cut(s) 139, 294
BstYI RGATCY 2 cut(s) 139, 294
BsuRI GGCC 1 cut(s) 315
BtrI CACGTC 1 cut(s) 42
CciI TCATGA 2 cut(s) 352, 586
CfoI GCGC 1 cut(s) 576
CseI GACGC 3 cut(s) 10, 290, 395
Csp6I GTAC 1 cut(s) 242
CviAII CATG 6 cut(s) 35, 78, 235, 245, 353, 587
CviJI RGCY 6 cut(s) 188, 203, 274, 315, 536, 569
CviKI_1 RGCY 6 cut(s) 188, 203, 274, 315, 536, 569
CviQI GTAC 1 cut(s) 242
DpnI GATC 3 cut(s) 141, 296, 527
DpnII GATC 3 cut(s) 139, 294, 525
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
Eco105I TACGTA 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 54, 204
Eco32I GATATC 1 cut(s) 562
EcoRII CCWGG 1 cut(s) 173
EcoRV GATATC 1 cut(s) 562
EcoT14I CCWWGG 2 cut(s) 54, 204
ErhI CCWWGG 2 cut(s) 54, 204
FaeI CATG 6 cut(s) 38, 81, 238, 248, 356, 590
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 1 cut(s) 389
FatI CATG 6 cut(s) 34, 77, 234, 244, 352, 586
FauI CCCGC 1 cut(s) 332
FblI GTMKAC 1 cut(s) 428
Fnu4HI GCNGC 3 cut(s) 228, 577, 596
Fsp4HI GCNGC 3 cut(s) 228, 577, 596
GlaI GCGC 1 cut(s) 575
GluI GCNGC 3 cut(s) 228, 577, 596
HaeIII GGCC 1 cut(s) 315
HapII CCGG 1 cut(s) 373
HgaI GACGC 3 cut(s) 10, 290, 395
HhaI GCGC 1 cut(s) 576
Hin1I GRCGYC 3 cut(s) 21, 301, 404
Hin1II CATG 6 cut(s) 38, 81, 238, 248, 356, 590
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HincII GTYRAC 2 cut(s) 168, 402
HindII GTYRAC 2 cut(s) 168, 402
HinfI GANTC 4 cut(s) 94, 364, 377, 509
HpaII CCGG 1 cut(s) 373
HphI GGTGA 1 cut(s) 496
Hpy166II GTNNAC 3 cut(s) 168, 402, 429
Hpy188I TCNGA 1 cut(s) 457
Hpy188III TCNNGA 5 cut(s) 292, 305, 353, 529, 587
Hpy8I GTNNAC 3 cut(s) 168, 402, 429
Hpy99I CGWCG 3 cut(s) 26, 46, 89
HpyAV CCTTC 4 cut(s) 83, 84, 452, 550
HpyCH4III ACNGT 1 cut(s) 426
HpyCH4IV ACGT 4 cut(s) 41, 65, 128, 404
HpyCH4V TGCA 4 cut(s) 156, 321, 411, 518
HpySE526I ACGT 4 cut(s) 41, 65, 128, 404
Hsp92I GRCGYC 3 cut(s) 21, 301, 404
Hsp92II CATG 6 cut(s) 38, 81, 238, 248, 356, 590
HspAI GCGC 1 cut(s) 574
Kzo9I GATC 3 cut(s) 139, 294, 525
LmnI GCTCC 2 cut(s) 31, 81
LweI GCATC 2 cut(s) 123, 165
MaeII ACGT 4 cut(s) 41, 65, 128, 404
MalI GATC 3 cut(s) 141, 296, 527
MboI GATC 3 cut(s) 139, 294, 525
MboII GAAGA 5 cut(s) 16, 19, 89, 190, 206
MflI RGATCY 2 cut(s) 139, 294
MluCI AATT 3 cut(s) 107, 347, 414
MlyI GAGTC 2 cut(s) 371, 518
MnlI CCTC 5 cut(s) 60, 354, 534, 546, 559
MroXI GAANNNNTTC 3 cut(s) 93, 348, 368
MspA1I CMGCKG 2 cut(s) 188, 339
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 2 cut(s) 175, 374
MvaI CCWGG 1 cut(s) 175
NciI CCSGG 1 cut(s) 374
NdeII GATC 3 cut(s) 139, 294, 525
NlaIII CATG 6 cut(s) 38, 81, 238, 248, 356, 590
NlaIV GGNNCC 1 cut(s) 141
NmeAIII GCCGAG 1 cut(s) 562
NspI RCATGY 2 cut(s) 238, 248
PagI TCATGA 2 cut(s) 352, 586
PciI ACATGT 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PdmI GAANNNNTTC 3 cut(s) 93, 348, 368
PfeI GAWTC 2 cut(s) 94, 364
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 3 cut(s) 229, 578, 597
PleI GAGTC 2 cut(s) 371, 517
PpsI GAGTC 2 cut(s) 371, 517
Ppu21I YACGTR 1 cut(s) 129
PscI ACATGT 1 cut(s) 244
Psp6I CCWGG 1 cut(s) 173
PspGI CCWGG 1 cut(s) 173
PspN4I GGNNCC 1 cut(s) 141
PsuI RGATCY 2 cut(s) 139, 294
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
SatI GCNGC 3 cut(s) 228, 577, 596
Sau3AI GATC 3 cut(s) 139, 294, 525
SchI GAGTC 2 cut(s) 371, 518
ScrFI CCNGG 2 cut(s) 175, 374
SetI ASST 8 cut(s) 44, 52, 60, 68, 131, 190, 401, 407
SfaNI GCATC 2 cut(s) 123, 165
SnaBI TACGTA 1 cut(s) 129
Sse9I AATT 3 cut(s) 107, 347, 414
SsiI CCGC 3 cut(s) 227, 339, 577
StyD4I CCNGG 2 cut(s) 173, 372
StyI CCWWGG 2 cut(s) 54, 204
TaaI ACNGT 1 cut(s) 426
TaiI ACGT 4 cut(s) 44, 68, 131, 407
TaqI TCGA 3 cut(s) 44, 121, 564
TasI AATT 3 cut(s) 107, 347, 414
TauI GCSGC 2 cut(s) 230, 579
TfiI GAWTC 2 cut(s) 94, 364
TseI GCWGC 1 cut(s) 595
TspDTI ATGAA 5 cut(s) 17, 66, 341, 369, 575
TspGWI ACGGA 1 cut(s) 373
XapI RAATTY 1 cut(s) 107
XceI RCATGY 2 cut(s) 238, 248
XmiI GTMKAC 1 cut(s) 428
XmnI GAANNNNTTC 3 cut(s) 93, 348, 368
ZraI GACGTC 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.