RLG00000027348

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012669
Physical Location & Seq
Reverse (-)
10830196 .. 10831028
833 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027348

Sequence Viewer

Length: 552 bp
ATGGTGAATTCAAGGTTGTTTTTGATCGGCAGACAACTTGCTCCCAACAACGACATGGATGATGATCCATCCGAAGTGGGTGTGTCGTTTTTGGATCTGAATCAACTAGAGGAGGCTTGGAGAGTCGCAGCCAGTATGTCAATCTGCGGGGGATCGAGGGCAGAGTTAACGCGATGTGTCACTGTCATCTGGAGATTAGTGGAACATTGGCTCACGAATATAAAGATGCGGTGGATGCATTGTCCGGTGCAAGGCCTGGTCATGAACAGTTACATGCTTCCATTCCAGAGGGATCAGGATGTTGGGATGACGGCTTTGATAAAAGTCGGTTCTCATCGATATGCACTCGTGTGGTCTCGCGAGTACACCGTTGAGGACTTTCAATTCGAAATTCATTGGTGTAAGTTTGATCTTCGACTGCATCACGAAGTCATTGAAGGAAAAACTGTATTGGATGATTACTTCTCAGCCGTATGGGTGGCACATGGGATGTTTAGGTTTGAAGATGTTGGATTTGGCACAACCTTGCTTAACATCACCTCCGATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

21.22

Weight (kDa)

5.28

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 172, 360
AciI CCGC 2 cut(s) 147, 229
AclWI GGATC 4 cut(s) 59, 102, 160, 300
AcsI RAATTY 2 cut(s) 7, 390
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 1 cut(s) 251
AgsI TTSAA 4 cut(s) 12, 383, 437, 503
AjnI CCWGG 1 cut(s) 255
AleI CACNNNNGTG 1 cut(s) 349
Alw26I GTCTC 1 cut(s) 360
AlwI GGATC 4 cut(s) 59, 102, 160, 300
AoxI GGCC 1 cut(s) 253
ApeKI GCWGC 1 cut(s) 128
ApoI RAATTY 2 cut(s) 7, 390
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AsuHPI GGTGA 2 cut(s) 16, 529
AsuII TTCGAA 1 cut(s) 387
BauI CACGAG 1 cut(s) 347
BbvI GCAGC 1 cut(s) 140
BccI CCATC 1 cut(s) 76
BceAI ACGGC 2 cut(s) 327, 455
BciT130I CCWGG 1 cut(s) 257
BcoDI GTCTC 1 cut(s) 360
BfaI CTAG 1 cut(s) 107
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
Bme1390I CCNGG 1 cut(s) 257
BmrFI CCNGG 1 cut(s) 257
BmsI GCATC 3 cut(s) 216, 225, 430
BpmI CTGGAG 1 cut(s) 211
Bpu14I TTCGAA 1 cut(s) 387
Bsa29I ATCGAT 1 cut(s) 337
BsaBI GATNNNNATC 2 cut(s) 63, 99
BsaI GGTCTC 1 cut(s) 360
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 1 cut(s) 524
Bsc4I CCNNNNNNNGG 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 132
Bse8I GATNNNNATC 2 cut(s) 63, 99
BseBI CCWGG 1 cut(s) 257
BseCI ATCGAT 1 cut(s) 337
BseGI GGATG 7 cut(s) 64, 68, 240, 304, 312, 460, 495
BseJI GATNNNNATC 2 cut(s) 63, 99
BseLI CCNNNNNNNGG 1 cut(s) 251
BseMII CTCAG 1 cut(s) 480
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 140
Bsh1236I CGCG 2 cut(s) 172, 360
BshFI GGCC 1 cut(s) 255
BshVI ATCGAT 1 cut(s) 337
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 251
BsmAI GTCTC 1 cut(s) 360
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 1 cut(s) 360
Bsp119I TTCGAA 1 cut(s) 387
Bsp143I GATC 6 cut(s) 24, 64, 94, 152, 292, 409
Bsp68I TCGCGA 1 cut(s) 360
BspACI CCGC 2 cut(s) 147, 229
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 1 cut(s) 479
BspDI ATCGAT 1 cut(s) 337
BspFNI CGCG 2 cut(s) 172, 360
BspHI TCATGA 1 cut(s) 261
BspPI GGATC 4 cut(s) 59, 102, 160, 300
BspT104I TTCGAA 1 cut(s) 387
BspTNI GGTCTC 1 cut(s) 360
BsrI ACTGG 1 cut(s) 132
BssMI GATC 6 cut(s) 24, 64, 94, 152, 292, 409
BssSI CACGAG 1 cut(s) 347
Bst2BI CACGAG 1 cut(s) 347
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 4 cut(s) 184, 269, 370, 448
BstBI TTCGAA 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 466
BstF5I GGATG 7 cut(s) 64, 68, 240, 304, 312, 460, 495
BstFNI CGCG 2 cut(s) 172, 360
BstKTI GATC 6 cut(s) 27, 67, 97, 155, 295, 412
BstMAI GTCTC 1 cut(s) 360
BstMBI GATC 6 cut(s) 24, 64, 94, 152, 292, 409
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstNI CCWGG 1 cut(s) 257
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 1 cut(s) 255
BstUI CGCG 2 cut(s) 172, 360
BstV1I GCAGC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
Bsu15I ATCGAT 1 cut(s) 337
BsuRI GGCC 1 cut(s) 255
BsuTUI ATCGAT 1 cut(s) 337
BtgZI GCGATG 1 cut(s) 187
BtsCI GGATG 7 cut(s) 64, 68, 240, 304, 312, 460, 495
BtsIMutI CAGTG 1 cut(s) 180
BtuMI TCGCGA 1 cut(s) 360
CciI TCATGA 1 cut(s) 261
ClaI ATCGAT 1 cut(s) 337
Csp6I GTAC 1 cut(s) 364
CviAII CATG 4 cut(s) 55, 262, 274, 485
CviJI RGCY 6 cut(s) 116, 131, 211, 255, 314, 470
CviKI_1 RGCY 6 cut(s) 116, 131, 211, 255, 314, 470
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 466
DpnI GATC 6 cut(s) 26, 66, 96, 154, 294, 411
DpnII GATC 6 cut(s) 24, 64, 94, 152, 292, 409
Eco147I AGGCCT 1 cut(s) 255
Eco31I GGTCTC 1 cut(s) 360
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 255
EcoT22I ATGCAT 1 cut(s) 240
FaeI CATG 4 cut(s) 58, 265, 277, 488
FaiI YATR 8 cut(s) 56, 137, 221, 263, 275, 342, 475, 486
FatI CATG 4 cut(s) 54, 261, 273, 484
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 7 cut(s) 55, 71, 247, 311, 319, 467, 502
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 1 cut(s) 107
GluI GCNGC 1 cut(s) 129
GsuI CTGGAG 1 cut(s) 211
HaeIII GGCC 1 cut(s) 255
HapII CCGG 1 cut(s) 245
Hin1II CATG 4 cut(s) 58, 265, 277, 488
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HinfI GANTC 2 cut(s) 100, 123
HpaI GTTAAC 1 cut(s) 168
HpaII CCGG 1 cut(s) 245
HphI GGTGA 2 cut(s) 16, 529
Hpy166II GTNNAC 2 cut(s) 168, 366
Hpy188I TCNGA 3 cut(s) 73, 99, 544
Hpy188III TCNNGA 7 cut(s) 190, 214, 262, 286, 296, 359, 425
Hpy8I GTNNAC 2 cut(s) 168, 366
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 4 cut(s) 184, 269, 370, 448
HpyCH4V TGCA 4 cut(s) 238, 250, 344, 421
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
HpyF3I CTNAG 1 cut(s) 466
Hsp92II CATG 4 cut(s) 58, 265, 277, 488
KspAI GTTAAC 1 cut(s) 168
Kzo9I GATC 6 cut(s) 24, 64, 94, 152, 292, 409
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 7 cut(s) 145, 175, 242, 258, 269, 281, 299
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 3 cut(s) 216, 225, 430
MaeI CTAG 1 cut(s) 107
MaeIII GTNAC 2 cut(s) 178, 269
MalI GATC 6 cut(s) 26, 66, 96, 154, 294, 411
MboI GATC 6 cut(s) 24, 64, 94, 152, 292, 409
MboII GAAGA 2 cut(s) 404, 515
MflI RGATCY 1 cut(s) 94
MluCI AATT 3 cut(s) 7, 383, 390
MlyI GAGTC 1 cut(s) 132
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 6 cut(s) 103, 106, 150, 282, 367, 550
Mph1103I ATGCAT 1 cut(s) 240
MseI TTAA 2 cut(s) 167, 531
MslI CAYNNNNRTG 2 cut(s) 339, 349
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 1 cut(s) 257
MvaI CCWGG 1 cut(s) 257
MvnI CGCG 2 cut(s) 172, 360
MwoI GCNNNNNNNGC 1 cut(s) 235
NdeII GATC 6 cut(s) 24, 64, 94, 152, 292, 409
NlaIII CATG 4 cut(s) 58, 265, 277, 488
NmuCI GTSAC 1 cut(s) 178
NruI TCGCGA 1 cut(s) 360
NsiI ATGCAT 1 cut(s) 240
NspI RCATGY 1 cut(s) 277
NspV TTCGAA 1 cut(s) 387
OliI CACNNNNGTG 1 cut(s) 349
PagI TCATGA 1 cut(s) 261
PceI AGGCCT 1 cut(s) 255
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 1 cut(s) 130
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PsuI RGATCY 1 cut(s) 94
RruI TCGCGA 1 cut(s) 360
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 2 cut(s) 339, 349
SaqAI TTAA 2 cut(s) 167, 531
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 6 cut(s) 24, 64, 94, 152, 292, 409
SchI GAGTC 1 cut(s) 132
ScrFI CCNGG 1 cut(s) 257
SetI ASST 4 cut(s) 17, 500, 527, 542
SfaNI GCATC 3 cut(s) 216, 225, 430
SfuI TTCGAA 1 cut(s) 387
SmiMI CAYNNNNRTG 2 cut(s) 339, 349
Sse9I AATT 3 cut(s) 7, 383, 390
SseBI AGGCCT 1 cut(s) 255
SsiI CCGC 2 cut(s) 147, 229
SspMI CTAG 1 cut(s) 107
StuI AGGCCT 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 255
TaaI ACNGT 4 cut(s) 184, 269, 370, 448
TaqI TCGA 4 cut(s) 155, 337, 387, 415
TasI AATT 3 cut(s) 7, 383, 390
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 2 cut(s) 167, 531
Tru9I TTAA 2 cut(s) 167, 531
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 2 cut(s) 278, 383
TspRI CASTG 1 cut(s) 187
XapI RAATTY 2 cut(s) 7, 390
XceI RCATGY 1 cut(s) 277
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XspI CTAG 1 cut(s) 107
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.