Rroxscaffold_4G00290210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012669
Physical Location & Seq
Reverse (-)
10747457 .. 10748907
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00290210.1

Sequence Viewer

Length: 552 bp
ATGGATGATGATACATCCGAAGTGGGTGTGCCATTTTTGGATCTGAATCAACTAGAGGAGGAGGCTTGGAGAGTCTCGGCCAAGTTCGCGGCGGGACAACGGGACTACGCGTTGGCTGCAGCGATGGTTGGATATCTTGCGTCGGTCACATCGGTGGGCGTTTTCACTCCCAAACCTTCCAATGGGAGCCGTCTCGACCTGGTCATGAACAGTTACATGCTTCCATTCCGGAGGGATGAGGATGTTGGGATGACGGCTTTGATAAAAGTCAGTCCTCATCGGCATACCGTGGATGGAGAAGTGCAGCATGATGACGATTACATTCGGCATAGGATGAAAGTTAGATATGTAGTCACAAAGTCATTTGAGATTGTTGTTGCTCTGGAGAAGATTGTTATTAATCCTCTTGACTGGGAATTTCATCATATTGGAGCTAATAGGAGAGTTGAACATCTCATTATAGAAACAGTTGCAAGAAATGGTGCTATGGACATCCCGAGAAAAGAAGTGCTTGAGCAACTCCAACAGATTCGTATAATTTTTGTCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.86

Weight (kDa)

5.63

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 89, 110
AccIII TCCGGA 1 cut(s) 228
AciI CCGC 2 cut(s) 89, 92
AclWI GGATC 1 cut(s) 48
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 182
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 1 cut(s) 449
AjnI CCWGG 1 cut(s) 198
AleI CACNNNNGTG 1 cut(s) 152
AluBI AGCT 1 cut(s) 434
AluI AGCT 1 cut(s) 434
Alw26I GTCTC 2 cut(s) 79, 197
AlwI GGATC 1 cut(s) 48
Ama87I CYCGRG 1 cut(s) 496
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 3 cut(s) 116, 119, 304
ApoI RAATTY 1 cut(s) 416
AseI ATTAAT 1 cut(s) 399
AvaI CYCGRG 1 cut(s) 496
BbvI GCAGC 3 cut(s) 103, 131, 316
BccI CCATC 2 cut(s) 118, 287
BceAI ACGGC 2 cut(s) 174, 270
BciT130I CCWGG 1 cut(s) 200
BcoDI GTCTC 2 cut(s) 79, 197
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 117
BisI GCNGC 4 cut(s) 90, 117, 120, 305
BlsI GCNGC 4 cut(s) 91, 118, 121, 306
Bme1390I CCNGG 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 496
BmiI GGNNCC 1 cut(s) 188
BmrFI CCNGG 1 cut(s) 200
BmrI ACTGGG 1 cut(s) 421
BmuI ACTGGG 1 cut(s) 421
BpmI CTGGAG 1 cut(s) 404
BpuEI CTTGAG 1 cut(s) 533
BsaBI GATNNNNATC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 416
Bse8I GATNNNNATC 1 cut(s) 45
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 8 cut(s) 10, 14, 241, 247, 255, 298, 339, 492
BseJI GATNNNNATC 1 cut(s) 45
BseLI CCNNNNNNNGG 1 cut(s) 182
BseNI ACTGG 1 cut(s) 416
BseRI GAGGAG 2 cut(s) 71, 74
BseXI GCAGC 3 cut(s) 103, 131, 316
BsgI GTGCAG 1 cut(s) 323
Bsh1236I CGCG 2 cut(s) 89, 110
BshFI GGCC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 496
BsiSI CCGG 1 cut(s) 229
BslFI GGGAC 2 cut(s) 108, 116
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 2 cut(s) 79, 197
BsmBI CGTCTC 1 cut(s) 197
BsmFI GGGAC 2 cut(s) 108, 116
BsnI GGCC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 496
Bsp13I TCCGGA 1 cut(s) 228
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 2 cut(s) 89, 92
BspANI GGCC 1 cut(s) 80
BspEI TCCGGA 1 cut(s) 228
BspFNI CGCG 2 cut(s) 89, 110
BspHI TCATGA 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 188
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 1 cut(s) 48
BsrI ACTGG 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 3 cut(s) 212, 289, 469
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 8 cut(s) 10, 14, 241, 247, 255, 298, 339, 492
BstFNI CGCG 2 cut(s) 89, 110
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 2 cut(s) 79, 197
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 86, 116
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 220
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 1 cut(s) 117
BstUI CGCG 2 cut(s) 89, 110
BstV1I GCAGC 3 cut(s) 103, 131, 316
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BsuRI GGCC 1 cut(s) 80
BtgI CCRYGG 1 cut(s) 288
BtgZI GCGATG 1 cut(s) 137
BtsCI GGATG 8 cut(s) 10, 14, 241, 247, 255, 298, 339, 492
CciI TCATGA 1 cut(s) 204
CseI GACGC 1 cut(s) 129
CsiI ACCWGGT 1 cut(s) 198
CviAII CATG 3 cut(s) 205, 217, 308
CviJI RGCY 6 cut(s) 65, 80, 116, 189, 257, 434
CviKI_1 RGCY 6 cut(s) 65, 80, 116, 189, 257, 434
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 78
Eco32I GATATC 1 cut(s) 134
Eco88I CYCGRG 1 cut(s) 496
EcoRII CCWGG 1 cut(s) 198
EcoRV GATATC 1 cut(s) 134
Esp3I CGTCTC 1 cut(s) 197
FaeI CATG 3 cut(s) 208, 220, 311
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 2 cut(s) 108, 116
FatI CATG 3 cut(s) 204, 216, 307
FauI CCCGC 1 cut(s) 85
Fnu4HI GCNGC 4 cut(s) 90, 117, 120, 305
FokI GGATG 7 cut(s) 17, 248, 254, 262, 305, 346, 479
Fsp4HI GCNGC 4 cut(s) 90, 117, 120, 305
FspBI CTAG 1 cut(s) 53
GluI GCNGC 4 cut(s) 90, 117, 120, 305
GsuI CTGGAG 1 cut(s) 404
HaeIII GGCC 1 cut(s) 80
HapII CCGG 1 cut(s) 229
HgaI GACGC 1 cut(s) 129
Hin1II CATG 3 cut(s) 208, 220, 311
HinfI GANTC 3 cut(s) 46, 72, 529
HpaII CCGG 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 19, 45
Hpy188III TCNNGA 6 cut(s) 194, 205, 229, 383, 407, 496
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 1 cut(s) 186
HpyCH4III ACNGT 3 cut(s) 212, 289, 469
HpyCH4V TGCA 3 cut(s) 119, 304, 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 116
Hsp92II CATG 3 cut(s) 208, 220, 311
Kpn2I TCCGGA 1 cut(s) 228
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 2 cut(s) 186, 431
LpnPI CCDG 5 cut(s) 185, 212, 242, 368, 397
Lsp1109I GCAGC 3 cut(s) 103, 131, 316
MabI ACCWGGT 1 cut(s) 198
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 3 cut(s) 145, 212, 352
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 1 cut(s) 400
MflI RGATCY 1 cut(s) 40
MluCI AATT 2 cut(s) 416, 537
MluI ACGCGT 1 cut(s) 108
MlyI GAGTC 1 cut(s) 81
MmeI TCCRAC 2 cut(s) 109, 547
MnlI CCTC 7 cut(s) 49, 52, 55, 225, 232, 285, 414
MroI TCCGGA 1 cut(s) 228
MseI TTAA 1 cut(s) 399
MslI CAYNNNNRTG 1 cut(s) 152
MspI CCGG 1 cut(s) 229
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 2 cut(s) 89, 110
MwoI GCNNNNNNNGC 2 cut(s) 86, 116
NdeII GATC 1 cut(s) 40
NlaIII CATG 3 cut(s) 208, 220, 311
NlaIV GGNNCC 1 cut(s) 188
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 2 cut(s) 145, 352
NspI RCATGY 1 cut(s) 220
OliI CACNNNNGTG 1 cut(s) 152
PagI TCATGA 1 cut(s) 204
PfeI GAWTC 2 cut(s) 46, 529
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 4 cut(s) 91, 118, 121, 306
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PshBI ATTAAT 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 188
PstI CTGCAG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 40
PsyI GACNNNGTC 1 cut(s) 200
RseI CAYNNNNRTG 1 cut(s) 152
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 4 cut(s) 90, 117, 120, 305
Sau3AI GATC 1 cut(s) 40
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 200
SetI ASST 3 cut(s) 178, 201, 436
SexAI ACCWGGT 1 cut(s) 198
SfcI CTRYAG 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 2 cut(s) 416, 537
SsiI CCGC 2 cut(s) 89, 92
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 198
TaaI ACNGT 3 cut(s) 212, 289, 469
TaqI TCGA 1 cut(s) 195
TaqII GACCGA 1 cut(s) 133
TasI AATT 2 cut(s) 416, 537
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 2 cut(s) 46, 529
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TseFI GTSAC 2 cut(s) 145, 352
TseI GCWGC 3 cut(s) 116, 119, 304
Tsp45I GTSAC 2 cut(s) 145, 352
TspDTI ATGAA 3 cut(s) 221, 350, 410
Tth111I GACNNNGTC 1 cut(s) 200
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 1 cut(s) 416
XceI RCATGY 1 cut(s) 220
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.