RLG00000028624

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0011387
Physical Location & Seq
Reverse (-)
25255011 .. 25257667
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028624

Sequence Viewer

Length: 1365 bp
ATGAGCCAGTGTGTTCCCAGCTGGGATCTTGATGACAGTAGTCCACCGGCGAGGCTCTCTCTTCGTTCTCACTCCAATTCCACCGCTCCTGATGTTCCCATGTTCGACTATGAACTAGCAGAGCTGACATGGGAAAATGGCCAGGTGGCCATGCACGGCTTGGGTCTACCGCGCTTGCCTTCCAAACCTGCATCCTGGGACAAGCCACGCGCTACTGGTACTCTGGAGTCCATAGTCAACCAAGCCACCTGTACTTTGCCTCGGTTTTCCAAGCCTCCCTTTGATAACACCATCACTTCTGCCACTGGCGGTGGTGCTAACAAAGCAGTGACCTGGTTTGATCATCACTGTCGTGTTGCCACCTCCGGTGCTCCCCCCAACACCATGACCATGGACGCGTTGGTCCCTTGCAGGAACAACCAACCTGATGAACCAAACAGTAGCCTTATGATAGACTCTGTAGTTGCCGTGCCAGGAGTGCTCGGCAGTTCTGTCACGGGCATGATCAATGGGTGCTCCACTCGAGTGGGGTCGTGCAGCGCTACCGCGACCCACAACGGGGACACCCGCCGACTGGGAAAACACAAGGCGGGCGTTGCACGTGTGCCTGCTACACCCGAGTGCAGATCCCCCAGCGTGAGCGGTAGTGCCACGTTTGGGATGGATAGCCAGCAAGCCACACTTGACACTAAGGGAAGCGACCATGACTCAGTTTGTCACAGTAGACCACAGAGAGAGGAAAGCGACGAGGAGGAGAGAAAGAAGCGAAGTACTGGAAAATCCTCGGTTTCTACAAAAAGGAGTAGGGCTGCTGCTATACATAACCAATCTGAACGTAAAAGGAGAGATAAGATCAACCAGAGGATGAAGACGCTGCAGAAGCTGGTCCCAAATTCCAGTAAGACTGATAAAGCTTCAATGCTGGACGAAGTGATTGAATACTTGAAACAATTGCAAGCTCAAGTCCATGTGATAAGCAGAATGAACATGCCAGCCATGATGTTGCCGATGGCTATGCAGCAACAACTTCAAATGTCTATGATGGCCCCAAGAAATATGGGCATGGGGATTGGGATGGGAATGGGAATGGGAATGGGAATGGACATGAACAACATCGGCCGCCCCAACATAGCGGGAGTCCCTCCGGTTCTTCACCCAACTGCATTCATGCCCATGGCTTCATGGGATGGCTCTGCCTGCGGCGCTGAGAGGTTACAAGCTCCTTCAACGACTGTGATGCCTGATCCCTTGCCAGCATTTCTTGCATGCCAATCGCAGCCGATGACCATGGATGCTTACAGCATGATGGCGGCTATGTATCAACAATTCCATCAGCAACCAGCAGCATCTAGTTCTAAGAGCTAA

Protein Analysis

455

Amino Acids

48.97

Weight (kDa)

8.77

Isoelectric Point (pI)

64.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 274 - 319 4.3e-14 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 277 - 324 1.9e-08 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011387)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00050 AT4G00050 AT4G00050
fragaria_vesca FvH4_7g10840
malus_domestica MD07G1113200.v1.1
prunus_persica Prupe.2G136300_v2.0.a1 Prupe.2G136300_v2.0.a1 Prupe.2G136300_v2.0.a1
pyrus_communis pycom07g09800
rosa_chinensis RchiOBHm_Chr1g0348781
rosa_laevigata RLG00000028624
rosa_multiflora Rmu_co8207186.1_g000001 Rmu_sc0002970.1_g000013
rosa_roxburghii Rroxscaffold_4G00306600
rosa_rugosa Rorug01G0197900
rosa_samantha Rh1AG215200 Rh1BG181500 Rh1CG199600 Rh1DG211400
rosa_wichuraiana Rw1G018230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 196
AccBSI CCGCTC 2 cut(s) 86, 642
AccI GTMKAC 2 cut(s) 166, 724
AccII CGCG 4 cut(s) 172, 210, 398, 548
AclWI GGATC 3 cut(s) 33, 621, 1238
AcoI YGGCCR 3 cut(s) 139, 147, 1117
AcsI RAATTY 1 cut(s) 892
AcvI CACGTG 1 cut(s) 602
AfaI GTAC 3 cut(s) 220, 253, 772
AfeI AGCGCT 1 cut(s) 541
AfiI CCNNNNNNNGG 4 cut(s) 558, 559, 574, 657
AflIII ACRYGT 2 cut(s) 396, 601
AgsI TTSAA 5 cut(s) 918, 938, 946, 1031, 1227
AjnI CCWGG 4 cut(s) 141, 194, 332, 472
AleI CACNNNNGTG 3 cut(s) 351, 524, 619
AluBI AGCT 7 cut(s) 21, 124, 883, 914, 959, 1220, 1362
AluI AGCT 7 cut(s) 21, 124, 883, 914, 959, 1220, 1362
Alw21I GWGCWC 3 cut(s) 373, 483, 518
AlwI GGATC 3 cut(s) 33, 621, 1238
AlwNI CAGNNNCTG 1 cut(s) 883
Ama87I CYCGRG 2 cut(s) 522, 617
Aor51HI AGCGCT 1 cut(s) 541
AoxI GGCC 4 cut(s) 139, 147, 1044, 1117
ApeKI GCWGC 7 cut(s) 537, 809, 812, 874, 1018, 1276, 1343
ApoI RAATTY 1 cut(s) 892
AspLEI GCGC 4 cut(s) 174, 212, 542, 1205
AspS9I GGNCC 3 cut(s) 403, 886, 1045
AsuHPI GGTGA 1 cut(s) 1145
AvaI CYCGRG 2 cut(s) 522, 617
AvaII GGWCC 2 cut(s) 403, 886
BalI TGGCCA 2 cut(s) 141, 149
BbrPI CACGTG 1 cut(s) 602
BbsI GAAGAC 1 cut(s) 875
Bbv12I GWGCWC 3 cut(s) 373, 483, 518
BbvI GCAGC 7 cut(s) 549, 796, 799, 861, 1030, 1288, 1355
BccI CCATC 8 cut(s) 299, 655, 1003, 1036, 1069, 1181, 1300, 1338
BceAI ACGGC 2 cut(s) 172, 452
BcgI CGANNNNNNTGC 6 cut(s) 997, 1031, 1219, 1253, 1254, 1288
BciT130I CCWGG 4 cut(s) 143, 196, 334, 474
BclI TGATCA 2 cut(s) 340, 504
BfaI CTAG 2 cut(s) 116, 1350
BfmI CTRYAG 2 cut(s) 459, 875
BfoI RGCGCY 2 cut(s) 543, 1206
BfuAI ACCTGC 1 cut(s) 196
BmcAI AGTACT 1 cut(s) 772
Bme1390I CCNGG 4 cut(s) 143, 196, 334, 474
Bme18I GGWCC 2 cut(s) 403, 886
BmeT110I CYCGRG 2 cut(s) 522, 617
BmgT120I GGNCC 3 cut(s) 403, 886, 1045
BmiI GGNNCC 3 cut(s) 405, 888, 1047
BmrFI CCNGG 4 cut(s) 143, 196, 334, 474
BmrI ACTGGG 1 cut(s) 584
BmsI GCATC 4 cut(s) 200, 1227, 1282, 1355
BmuI ACTGGG 1 cut(s) 584
BoxI GACNNNNGTC 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 875
BplI GAGNNNNNCTC 2 cut(s) 43, 75
BpmI CTGGAG 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 945
BsaAI YACGTR 1 cut(s) 602
BsaJI CCNNGG 6 cut(s) 195, 260, 390, 783, 1173, 1287
BsaWI WCCGGW 2 cut(s) 365, 1144
Bsc4I CCNNNNNNNGG 4 cut(s) 558, 559, 574, 657
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 6 cut(s) 7, 220, 310, 579, 778, 897
BseBI CCWGG 4 cut(s) 143, 196, 334, 474
BseDI CCNNGG 6 cut(s) 195, 260, 390, 783, 1173, 1287
BseGI GGATG 6 cut(s) 191, 666, 870, 1080, 1192, 1297
BseLI CCNNNNNNNGG 4 cut(s) 558, 559, 574, 657
BseMII CTCAG 2 cut(s) 723, 1197
BseNI ACTGG 6 cut(s) 7, 220, 310, 579, 778, 897
BseRI GAGGAG 2 cut(s) 764, 767
BseX3I CGGCCG 1 cut(s) 1117
BseXI GCAGC 7 cut(s) 549, 796, 799, 861, 1030, 1288, 1355
BseYI CCCAGC 3 cut(s) 17, 21, 632
BsgI GTGCAG 2 cut(s) 556, 643
Bsh1236I CGCG 4 cut(s) 172, 210, 398, 548
Bsh1285I CGRYCG 1 cut(s) 1120
BshFI GGCC 4 cut(s) 141, 149, 1046, 1119
BsiEI CGRYCG 1 cut(s) 1120
BsiHKAI GWGCWC 3 cut(s) 373, 483, 518
BsiHKCI CYCGRG 2 cut(s) 522, 617
BsiSI CCGG 3 cut(s) 47, 366, 1145
BslFI GGGAC 5 cut(s) 212, 389, 575, 872, 1124
BslI CCNNNNNNNGG 4 cut(s) 558, 559, 574, 657
BsmFI GGGAC 5 cut(s) 212, 389, 575, 872, 1124
BsmI GAATGC 1 cut(s) 1163
BsnI GGCC 4 cut(s) 141, 149, 1046, 1119
BsoBI CYCGRG 2 cut(s) 522, 617
Bsp1286I GDGCHC 3 cut(s) 373, 483, 518
Bsp143I GATC 6 cut(s) 25, 340, 504, 626, 852, 1243
Bsp19I CCATGG 3 cut(s) 390, 1173, 1287
BspANI GGCC 4 cut(s) 141, 149, 1046, 1119
BspCNI CTCAG 2 cut(s) 722, 1198
BspFNI CGCG 4 cut(s) 172, 210, 398, 548
BspLI GGNNCC 3 cut(s) 405, 888, 1047
BspMAI CTGCAG 1 cut(s) 879
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 3 cut(s) 33, 621, 1238
BsrBI CCGCTC 2 cut(s) 86, 642
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 6 cut(s) 7, 220, 310, 579, 778, 897
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 6 cut(s) 195, 260, 390, 783, 1173, 1287
BssMI GATC 6 cut(s) 25, 340, 504, 626, 852, 1243
BssT1I CCWWGG 3 cut(s) 390, 1173, 1287
Bst2UI CCWGG 4 cut(s) 143, 196, 334, 474
Bst4CI ACNGT 5 cut(s) 38, 350, 440, 722, 1234
Bst6I CTCTTC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstBAI YACGTR 1 cut(s) 602
BstDEI CTNAG 4 cut(s) 690, 709, 1206, 1356
BstDSI CCRYGG 3 cut(s) 390, 1173, 1287
BstF5I GGATG 6 cut(s) 191, 666, 870, 1080, 1192, 1297
BstFNI CGCG 4 cut(s) 172, 210, 398, 548
BstH2I RGCGCY 2 cut(s) 543, 1206
BstHHI GCGC 4 cut(s) 174, 212, 542, 1205
BstKTI GATC 6 cut(s) 28, 343, 507, 629, 855, 1246
BstMBI GATC 6 cut(s) 25, 340, 504, 626, 852, 1243
BstMCI CGRYCG 1 cut(s) 1120
BstMWI GCNNNNNNNGC 7 cut(s) 323, 478, 596, 880, 1197, 1202, 1262
BstNI CCWGG 4 cut(s) 143, 196, 334, 474
BstNSI RCATGY 2 cut(s) 991, 1269
BstPAI GACNNNNGTC 1 cut(s) 39
BstSCI CCNGG 4 cut(s) 141, 194, 332, 472
BstSFI CTRYAG 2 cut(s) 459, 875
BstUI CGCG 4 cut(s) 172, 210, 398, 548
BstV1I GCAGC 7 cut(s) 549, 796, 799, 861, 1030, 1288, 1355
BstV2I GAAGAC 1 cut(s) 875
BstX2I RGATCY 2 cut(s) 25, 626
BstXI CCANNNNNNTGG 2 cut(s) 391, 526
BstYI RGATCY 2 cut(s) 25, 626
BstZI CGGCCG 1 cut(s) 1117
BsuRI GGCC 4 cut(s) 141, 149, 1046, 1119
BtgI CCRYGG 3 cut(s) 390, 1173, 1287
BtsCI GGATG 6 cut(s) 191, 666, 870, 1080, 1192, 1297
BtsI GCAGTG 1 cut(s) 333
BtsIMutI CAGTG 4 cut(s) 14, 303, 333, 346
BveI ACCTGC 1 cut(s) 196
CaiI CAGNNNCTG 1 cut(s) 883
CfoI GCGC 4 cut(s) 174, 212, 542, 1205
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 3 cut(s) 403, 886, 1045
CseI GACGC 2 cut(s) 404, 880
CsiI ACCWGGT 1 cut(s) 332
Csp6I GTAC 3 cut(s) 219, 252, 771
CviQI GTAC 3 cut(s) 219, 252, 771
DdeI CTNAG 4 cut(s) 690, 709, 1206, 1356
DpnI GATC 6 cut(s) 27, 342, 506, 628, 854, 1245
DpnII GATC 6 cut(s) 25, 340, 504, 626, 852, 1243
DrdI GACNNNNNNGTC 1 cut(s) 401
DseDI GACNNNNNNGTC 1 cut(s) 401
EaeI YGGCCR 3 cut(s) 139, 147, 1117
EagI CGGCCG 1 cut(s) 1117
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
EclXI CGGCCG 1 cut(s) 1117
Eco130I CCWWGG 3 cut(s) 390, 1173, 1287
Eco47I GGWCC 2 cut(s) 403, 886
Eco47III AGCGCT 1 cut(s) 541
Eco52I CGGCCG 1 cut(s) 1117
Eco72I CACGTG 1 cut(s) 602
Eco88I CYCGRG 2 cut(s) 522, 617
EcoRII CCWGG 4 cut(s) 141, 194, 332, 472
EcoT14I CCWWGG 3 cut(s) 390, 1173, 1287
ErhI CCWWGG 3 cut(s) 390, 1173, 1287
FalI AAGNNNNNCTT 4 cut(s) 263, 295, 666, 698
FaqI GGGAC 5 cut(s) 212, 389, 575, 872, 1124
FauI CCCGC 3 cut(s) 575, 583, 1126
FbaI TGATCA 2 cut(s) 340, 504
FblI GTMKAC 2 cut(s) 166, 724
FokI GGATG 6 cut(s) 178, 673, 877, 1087, 1199, 1304
FspBI CTAG 2 cut(s) 116, 1350
GlaI GCGC 4 cut(s) 173, 211, 541, 1204
GsaI CCCAGC 3 cut(s) 21, 25, 636
GsuI CTGGAG 1 cut(s) 245
HaeII RGCGCY 2 cut(s) 543, 1206
HaeIII GGCC 4 cut(s) 141, 149, 1046, 1119
HapII CCGG 3 cut(s) 47, 366, 1145
HgaI GACGC 2 cut(s) 404, 880
HhaI GCGC 4 cut(s) 174, 212, 542, 1205
Hin6I GCGC 4 cut(s) 172, 210, 540, 1203
HinP1I GCGC 4 cut(s) 172, 210, 540, 1203
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HindIII AAGCTT 1 cut(s) 912
HinfI GANTC 4 cut(s) 227, 455, 707, 1137
HpaII CCGG 3 cut(s) 47, 366, 1145
HphI GGTGA 1 cut(s) 1145
Hpy166II GTNNAC 4 cut(s) 44, 167, 238, 725
Hpy188I TCNGA 1 cut(s) 832
Hpy188III TCNNGA 3 cut(s) 29, 89, 224
Hpy8I GTNNAC 4 cut(s) 44, 167, 238, 725
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 189, 1233
HpyCH4III ACNGT 5 cut(s) 38, 350, 440, 722, 1234
HpyCH4IV ACGT 3 cut(s) 601, 653, 835
HpyF10VI GCNNNNNNNGC 7 cut(s) 323, 478, 596, 880, 1197, 1202, 1262
HpyF3I CTNAG 4 cut(s) 690, 709, 1206, 1356
HpySE526I ACGT 3 cut(s) 601, 653, 835
HspAI GCGC 4 cut(s) 172, 210, 540, 1203
Ksp22I TGATCA 2 cut(s) 340, 504
Kzo9I GATC 6 cut(s) 25, 340, 504, 626, 852, 1243
LmnI GCTCC 4 cut(s) 91, 376, 521, 1225
Lsp1109I GCAGC 7 cut(s) 549, 796, 799, 861, 1030, 1288, 1355
LweI GCATC 4 cut(s) 200, 1227, 1282, 1355
MabI ACCWGGT 1 cut(s) 332
MaeI CTAG 2 cut(s) 116, 1350
MaeII ACGT 3 cut(s) 601, 653, 835
MaeIII GTNAC 4 cut(s) 328, 493, 716, 1212
MalI GATC 6 cut(s) 27, 342, 506, 628, 854, 1245
MbiI CCGCTC 2 cut(s) 86, 642
MboI GATC 6 cut(s) 25, 340, 504, 626, 852, 1243
MboII GAAGA 3 cut(s) 53, 880, 1142
MfeI CAATTG 1 cut(s) 950
MflI RGATCY 2 cut(s) 25, 626
MhlI GDGCHC 3 cut(s) 373, 483, 518
MlsI TGGCCA 2 cut(s) 141, 149
MluCI AATT 4 cut(s) 76, 892, 950, 1325
MluI ACGCGT 1 cut(s) 396
MluNI TGGCCA 2 cut(s) 141, 149
MlyI GAGTC 4 cut(s) 236, 449, 701, 1146
Mox20I TGGCCA 2 cut(s) 141, 149
MscI TGGCCA 2 cut(s) 141, 149
MslI CAYNNNNRTG 6 cut(s) 351, 389, 500, 524, 619, 1172
Msp20I TGGCCA 2 cut(s) 141, 149
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 3 cut(s) 47, 366, 1145
MspR9I CCNGG 4 cut(s) 143, 196, 334, 474
MunI CAATTG 1 cut(s) 950
Mva1269I GAATGC 1 cut(s) 1163
MvaI CCWGG 4 cut(s) 143, 196, 334, 474
MvnI CGCG 4 cut(s) 172, 210, 398, 548
MwoI GCNNNNNNNGC 7 cut(s) 323, 478, 596, 880, 1197, 1202, 1262
NcoI CCATGG 3 cut(s) 390, 1173, 1287
NdeII GATC 6 cut(s) 25, 340, 504, 626, 852, 1243
NlaIV GGNNCC 3 cut(s) 405, 888, 1047
NmeAIII GCCGAG 1 cut(s) 462
NmuCI GTSAC 3 cut(s) 328, 493, 716
NspI RCATGY 2 cut(s) 991, 1269
OliI CACNNNNGTG 3 cut(s) 351, 524, 619
PaeI GCATGC 1 cut(s) 1269
PaeR7I CTCGAG 1 cut(s) 522
PctI GAATGC 1 cut(s) 1163
PleI GAGTC 4 cut(s) 235, 449, 701, 1145
PmaCI CACGTG 1 cut(s) 602
PmlI CACGTG 1 cut(s) 602
PpsI GAGTC 4 cut(s) 235, 449, 701, 1145
Ppu21I YACGTR 1 cut(s) 602
PshAI GACNNNNGTC 1 cut(s) 39
Psp6I CCWGG 4 cut(s) 141, 194, 332, 472
PspCI CACGTG 1 cut(s) 602
PspFI CCCAGC 3 cut(s) 17, 21, 632
PspGI CCWGG 4 cut(s) 141, 194, 332, 472
PspN4I GGNNCC 3 cut(s) 405, 888, 1047
PspPI GGNCC 3 cut(s) 403, 886, 1045
PspXI VCTCGAGB 1 cut(s) 522
PstI CTGCAG 1 cut(s) 879
PstNI CAGNNNCTG 1 cut(s) 883
PsuI RGATCY 2 cut(s) 25, 626
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 3 cut(s) 220, 253, 772
RsaNI GTAC 3 cut(s) 219, 252, 771
RseI CAYNNNNRTG 6 cut(s) 351, 389, 500, 524, 619, 1172
Sau3AI GATC 6 cut(s) 25, 340, 504, 626, 852, 1243
Sau96I GGNCC 3 cut(s) 403, 886, 1045
ScaI AGTACT 1 cut(s) 772
SchI GAGTC 4 cut(s) 236, 449, 701, 1146
ScrFI CCNGG 4 cut(s) 143, 196, 334, 474
SduI GDGCHC 3 cut(s) 373, 483, 518
SexAI ACCWGGT 1 cut(s) 332
SfaNI GCATC 4 cut(s) 200, 1227, 1282, 1355
SfcI CTRYAG 2 cut(s) 459, 875
Sfr274I CTCGAG 1 cut(s) 522
SgrAI CRCCGGYG 1 cut(s) 46
SinI GGWCC 2 cut(s) 403, 886
SlaI CTCGAG 1 cut(s) 522
SmiMI CAYNNNNRTG 6 cut(s) 351, 389, 500, 524, 619, 1172
SmlI CTYRAG 2 cut(s) 522, 960
SmoI CTYRAG 2 cut(s) 522, 960
SphI GCATGC 1 cut(s) 1269
Sse9I AATT 4 cut(s) 76, 892, 950, 1325
SspMI CTAG 2 cut(s) 116, 1350
StyD4I CCNGG 4 cut(s) 141, 194, 332, 472
StyI CCWWGG 3 cut(s) 390, 1173, 1287
TaaI ACNGT 5 cut(s) 38, 350, 440, 722, 1234
TaiI ACGT 3 cut(s) 604, 656, 838
TaqI TCGA 2 cut(s) 105, 523
TasI AATT 4 cut(s) 76, 892, 950, 1325
TatI WGTACW 2 cut(s) 251, 770
TauI GCSGC 3 cut(s) 1122, 1203, 1313
TscAI CASTG 4 cut(s) 14, 310, 333, 353
TseFI GTSAC 3 cut(s) 328, 493, 716
TseI GCWGC 7 cut(s) 537, 809, 812, 874, 1018, 1276, 1343
Tsp45I GTSAC 3 cut(s) 328, 493, 716
TspDTI ATGAA 7 cut(s) 126, 444, 881, 998, 1121, 1156, 1170
TspRI CASTG 4 cut(s) 14, 310, 333, 353
VpaK11BI GGWCC 2 cut(s) 403, 886
XapI RAATTY 1 cut(s) 892
XceI RCATGY 2 cut(s) 991, 1269
XcmI CCANNNNNNNNNTGG 3 cut(s) 157, 397, 658
XhoI CTCGAG 1 cut(s) 522
XmiI GTMKAC 2 cut(s) 166, 724
XspI CTAG 2 cut(s) 116, 1350
ZrmI AGTACT 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.