RLG00000031975

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015571
Physical Location & Seq
Forward (+)
9938497 .. 9942649
4153 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031975

Sequence Viewer

Length: 1089 bp
ATGATAAGACACTATTTCCATAGCGAAGTGTGGCAACCAGAAACCCTCACTGAGGTTTCAACTCCAAGCTGCAGCGACCTGTTCCCGATCAAATTCCAGCTCTTCGAGGTCACCAGAAGCAGCAGTCGGACAGAAGAAGAAGATGAAAATGCTACTAGTGAGGAATTAGAAGAAGCAAAGCTTGTAGCCGAAAGACAAACTAAACCACTCTCTCGTGCCCAGCCATTGTGTTTGGGGACTCAGGACACAAGGGTAATCTGCACAGGCACTCTTTCCAGCATGGGCGTCCCACGAGATGAGCAGCCTGCTATTGTTGCAAAGATATTCGAGGCCTTGAAAGTCGCCGTCCCCTTCCGGCCTATCTGCGTGGTCATCAAGGACATTGTGAGCGTGCAAGAGCGCAGTGGTGGGGATGAAAGTTCGAACAAAGTGAGATTTGATAGCTTGGAAGTTAAGGAAGAGGAACAGGAGGAGGAGGAGGAGTGTCACCAATACATATATGAAGAAGATATCGACACGGACCCGCTACCCGAACCCTTGTGGTGCGAGGGCGGGTTGATTGAGGTGGCTGCTGGCTGGACTTCGGTGGATGTTGCGGAGGTGGTCGACGAGTCATCAAGACCCTCTAGCCCCAGACGAGACGAAAACAACCAACACGATGATATGCATCGGCCTGTCGCGGGGGGGCCATCATTACCATCAAGTTCTCTACAAGAGTACTGGTGGAACTACCCACCAAACTCAAGTCCGCAGTTGGCCTTTGTGGGCGAGCCATTAGTACCGTTAGCCCACCCGCCAGGATCATTGTTATGGCTCGCCGGCGTGCAGCCATCTGGACCCTCGAGGCTGCAGTGGGATATGTATAAGGTTCTCATGTTGGCTGGGAGGTTGATGCTTGTTTACGTCTTGTGCTACCTTTTAATTGAGCAGCTAACTAAGCCGCTTGTTGAGTACATCTTTCATGCGCTCGCGTATGCCTTTCAGGCGCTGCTCATGTATGTTCTTCATGCACTGCTCATCTATGTCATACCTATTACTTTGGTCATCATCTTCTTCAGAATCATCTTCTTTGGATACAAGCAGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

363

Amino Acids

41.08

Weight (kDa)

4.67

Isoelectric Point (pI)

68.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 841
AccI GTMKAC 1 cut(s) 606
AccII CGCG 2 cut(s) 680, 971
AciI CCGC 7 cut(s) 524, 552, 596, 680, 749, 794, 941
AclWI GGATC 1 cut(s) 808
AcsI RAATTY 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 1039
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 3 cut(s) 719, 780, 953
AfeI AGCGCT 1 cut(s) 1085
AfiI CCNNNNNNNGG 2 cut(s) 52, 680
AgsI TTSAA 2 cut(s) 60, 337
AhlI ACTAGT 1 cut(s) 155
AjnI CCWGG 1 cut(s) 796
AluBI AGCT 5 cut(s) 69, 100, 181, 444, 931
AluI AGCT 5 cut(s) 69, 100, 181, 444, 931
Alw26I GTCTC 1 cut(s) 633
AlwI GGATC 1 cut(s) 808
AlwNI CAGNNNCTG 1 cut(s) 988
Ama87I CYCGRG 1 cut(s) 841
Aor51HI AGCGCT 1 cut(s) 1085
AoxI GGCC 5 cut(s) 330, 356, 671, 686, 756
ApoI RAATTY 1 cut(s) 92
AspLEI GCGC 4 cut(s) 402, 967, 988, 1086
AspS9I GGNCC 3 cut(s) 520, 686, 836
AsuHPI GGTGA 2 cut(s) 103, 479
AsuII TTCGAA 1 cut(s) 422
AvaI CYCGRG 1 cut(s) 841
AvaII GGWCC 2 cut(s) 520, 836
BaeGI GKGCMC 1 cut(s) 220
BauI CACGAG 2 cut(s) 213, 291
BbvI GCAGC 9 cut(s) 56, 84, 132, 313, 556, 834, 838, 940, 975
BccI CCATC 3 cut(s) 697, 706, 838
BceAI ACGGC 1 cut(s) 329
BciT130I CCWGG 1 cut(s) 798
BciVI GTATCC 1 cut(s) 1067
BcoDI GTCTC 1 cut(s) 633
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 3 cut(s) 156, 627, 1087
BfmI CTRYAG 2 cut(s) 70, 848
BfoI RGCGCY 2 cut(s) 989, 1087
BfuI GTATCC 1 cut(s) 1067
BglI GCCNNNNNGGC 1 cut(s) 983
BmcAI AGTACT 1 cut(s) 719
Bme1390I CCNGG 1 cut(s) 798
Bme18I GGWCC 2 cut(s) 520, 836
BmeT110I CYCGRG 1 cut(s) 841
BmgT120I GGNCC 3 cut(s) 520, 686, 836
BmiI GGNNCC 3 cut(s) 522, 687, 838
BmrFI CCNGG 1 cut(s) 798
BmsI GCATC 2 cut(s) 676, 882
Bpu14I TTCGAA 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 727
BsaBI GATNNNNATC 1 cut(s) 666
BsaHI GRCGYC 1 cut(s) 285
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 680
Bse118I RCCGGY 1 cut(s) 818
Bse1I ACTGG 1 cut(s) 725
Bse8I GATNNNNATC 1 cut(s) 666
BseBI CCWGG 1 cut(s) 798
BseGI GGATG 2 cut(s) 418, 595
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 2 cut(s) 52, 680
BseMII CTCAG 2 cut(s) 42, 254
BseNI ACTGG 1 cut(s) 725
BseRI GAGGAG 4 cut(s) 485, 488, 491, 494
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 9 cut(s) 56, 84, 132, 313, 556, 834, 838, 940, 975
BseYI CCCAGC 2 cut(s) 219, 881
BsgI GTGCAG 2 cut(s) 244, 845
Bsh1236I CGCG 2 cut(s) 680, 971
BshFI GGCC 5 cut(s) 332, 358, 673, 688, 758
BsiHKCI CYCGRG 1 cut(s) 841
BsiSI CCGG 2 cut(s) 355, 819
BslFI GGGAC 3 cut(s) 250, 272, 332
BslI CCNNNNNNNGG 2 cut(s) 52, 680
BsmAI GTCTC 1 cut(s) 633
BsmBI CGTCTC 1 cut(s) 633
BsmFI GGGAC 3 cut(s) 250, 272, 332
BsnI GGCC 5 cut(s) 332, 358, 673, 688, 758
BsoBI CYCGRG 1 cut(s) 841
Bsp119I TTCGAA 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 2 cut(s) 87, 800
BspACI CCGC 7 cut(s) 524, 552, 596, 680, 749, 794, 941
BspANI GGCC 5 cut(s) 332, 358, 673, 688, 758
BspCNI CTCAG 2 cut(s) 43, 253
BspFNI CGCG 2 cut(s) 680, 971
BspLI GGNNCC 3 cut(s) 522, 687, 838
BspMAI CTGCAG 2 cut(s) 74, 852
BspPI GGATC 1 cut(s) 808
BspQI GCTCTTC 1 cut(s) 107
BspT104I TTCGAA 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 818
BsrI ACTGG 1 cut(s) 725
BssAI RCCGGY 1 cut(s) 818
BssMI GATC 2 cut(s) 87, 800
BssNI GRCGYC 1 cut(s) 285
BssSI CACGAG 2 cut(s) 213, 291
Bst2BI CACGAG 2 cut(s) 213, 291
Bst2UI CCWGG 1 cut(s) 798
Bst4CI ACNGT 1 cut(s) 783
Bst6I CTCTTC 2 cut(s) 107, 453
BstACI GRCGYC 1 cut(s) 285
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 8 cut(s) 306, 392, 574, 770, 816, 820, 824, 969
BstDEI CTNAG 3 cut(s) 51, 240, 936
BstEII GGTNACC 1 cut(s) 109
BstENI CCTNNNNNAGG 1 cut(s) 50
BstF5I GGATG 2 cut(s) 418, 595
BstFNI CGCG 2 cut(s) 680, 971
BstH2I RGCGCY 2 cut(s) 989, 1087
BstHHI GCGC 4 cut(s) 402, 967, 988, 1086
BstKTI GATC 2 cut(s) 90, 803
BstMAI GTCTC 1 cut(s) 633
BstMBI GATC 2 cut(s) 87, 800
BstMWI GCNNNNNNNGC 3 cut(s) 314, 937, 983
BstNI CCWGG 1 cut(s) 798
BstPI GGTNACC 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 796
BstSFI CTRYAG 2 cut(s) 70, 848
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 2 cut(s) 680, 971
BstV1I GCAGC 9 cut(s) 56, 84, 132, 313, 556, 834, 838, 940, 975
BsuI GTATCC 1 cut(s) 1067
BsuRI GGCC 5 cut(s) 332, 358, 673, 688, 758
BtsCI GGATG 2 cut(s) 418, 595
BtsI GCAGTG 3 cut(s) 409, 857, 1010
BtsIMutI CAGTG 4 cut(s) 48, 409, 857, 1010
Cac8I GCNNGC 8 cut(s) 306, 392, 574, 770, 816, 820, 824, 969
CaiI CAGNNNCTG 1 cut(s) 988
CfoI GCGC 4 cut(s) 402, 967, 988, 1086
Cfr10I RCCGGY 1 cut(s) 818
Cfr13I GGNCC 3 cut(s) 520, 686, 836
CseI GACGC 1 cut(s) 274
Csp6I GTAC 3 cut(s) 718, 779, 952
CviAII CATG 5 cut(s) 280, 874, 962, 994, 1007
CviQI GTAC 3 cut(s) 718, 779, 952
DdeI CTNAG 3 cut(s) 51, 240, 936
DpnI GATC 2 cut(s) 89, 802
DpnII GATC 2 cut(s) 87, 800
Eam1104I CTCTTC 2 cut(s) 107, 453
EarI CTCTTC 2 cut(s) 107, 453
Eco147I AGGCCT 1 cut(s) 332
Eco32I GATATC 1 cut(s) 511
Eco47I GGWCC 2 cut(s) 520, 836
Eco47III AGCGCT 1 cut(s) 1085
Eco57I CTGAAG 1 cut(s) 1039
Eco88I CYCGRG 1 cut(s) 841
Eco91I GGTNACC 1 cut(s) 109
EcoNI CCTNNNNNAGG 1 cut(s) 50
EcoO65I GGTNACC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 796
EcoRV GATATC 1 cut(s) 511
EcoT22I ATGCAT 1 cut(s) 669
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 5 cut(s) 283, 877, 965, 997, 1010
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FaqI GGGAC 3 cut(s) 250, 272, 332
FatI CATG 5 cut(s) 279, 873, 961, 993, 1006
FauI CCCGC 4 cut(s) 531, 545, 673, 801
FblI GTMKAC 1 cut(s) 606
FokI GGATG 2 cut(s) 425, 602
FspBI CTAG 3 cut(s) 156, 627, 1087
GlaI GCGC 4 cut(s) 401, 966, 987, 1085
GsaI CCCAGC 2 cut(s) 223, 885
HaeII RGCGCY 2 cut(s) 989, 1087
HaeIII GGCC 5 cut(s) 332, 358, 673, 688, 758
HapII CCGG 2 cut(s) 355, 819
HgaI GACGC 1 cut(s) 274
HhaI GCGC 4 cut(s) 402, 967, 988, 1086
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 5 cut(s) 283, 877, 965, 997, 1010
Hin6I GCGC 4 cut(s) 400, 965, 986, 1084
HinP1I GCGC 4 cut(s) 400, 965, 986, 1084
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HindIII AAGCTT 1 cut(s) 179
HinfI GANTC 3 cut(s) 238, 611, 1059
HpaII CCGG 2 cut(s) 355, 819
HphI GGTGA 2 cut(s) 103, 479
Hpy166II GTNNAC 2 cut(s) 607, 901
Hpy188I TCNGA 2 cut(s) 129, 1058
Hpy188III TCNNGA 4 cut(s) 85, 242, 618, 834
Hpy8I GTNNAC 2 cut(s) 607, 901
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 1 cut(s) 783
HpyCH4IV ACGT 1 cut(s) 903
HpyCH4V TGCA 8 cut(s) 72, 261, 317, 394, 667, 826, 850, 1010
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 937, 983
HpyF3I CTNAG 3 cut(s) 51, 240, 936
HpySE526I ACGT 1 cut(s) 903
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 5 cut(s) 283, 877, 965, 997, 1010
HspAI GCGC 4 cut(s) 400, 965, 986, 1084
KroI GCCGGC 1 cut(s) 818
KroNI GCCGGC 1 cut(s) 820
Kzo9I GATC 2 cut(s) 87, 800
LguI GCTCTTC 1 cut(s) 107
Lsp1109I GCAGC 9 cut(s) 56, 84, 132, 313, 556, 834, 838, 940, 975
LweI GCATC 2 cut(s) 676, 882
MaeI CTAG 3 cut(s) 156, 627, 1087
MaeII ACGT 1 cut(s) 903
MaeIII GTNAC 2 cut(s) 109, 485
MalI GATC 2 cut(s) 89, 802
MboI GATC 2 cut(s) 87, 800
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 3 cut(s) 92, 164, 921
MlyI GAGTC 2 cut(s) 232, 620
MmeI TCCRAC 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 669
MreI CGCCGGCG 1 cut(s) 818
MroNI GCCGGC 1 cut(s) 818
MseI TTAA 2 cut(s) 453, 920
MslI CAYNNNNRTG 1 cut(s) 808
MspI CCGG 2 cut(s) 355, 819
MspR9I CCNGG 1 cut(s) 798
MvaI CCWGG 1 cut(s) 798
MvnI CGCG 2 cut(s) 680, 971
MwoI GCNNNNNNNGC 3 cut(s) 314, 937, 983
NaeI GCCGGC 1 cut(s) 820
NdeII GATC 2 cut(s) 87, 800
NgoMIV GCCGGC 1 cut(s) 818
NlaIII CATG 5 cut(s) 283, 877, 965, 997, 1010
NlaIV GGNNCC 3 cut(s) 522, 687, 838
NmuCI GTSAC 2 cut(s) 109, 485
NsiI ATGCAT 1 cut(s) 669
NspV TTCGAA 1 cut(s) 422
PaeR7I CTCGAG 1 cut(s) 841
PceI AGGCCT 1 cut(s) 332
PciSI GCTCTTC 1 cut(s) 107
PdiI GCCGGC 1 cut(s) 820
PfeI GAWTC 1 cut(s) 1059
PleI GAGTC 2 cut(s) 232, 619
PpsI GAGTC 2 cut(s) 232, 619
Psp6I CCWGG 1 cut(s) 796
PspEI GGTNACC 1 cut(s) 109
PspFI CCCAGC 2 cut(s) 219, 881
PspGI CCWGG 1 cut(s) 796
PspN4I GGNNCC 3 cut(s) 522, 687, 838
PspPI GGNCC 3 cut(s) 520, 686, 836
PspXI VCTCGAGB 1 cut(s) 841
PstI CTGCAG 2 cut(s) 74, 852
PstNI CAGNNNCTG 1 cut(s) 988
RsaI GTAC 3 cut(s) 719, 780, 953
RsaNI GTAC 3 cut(s) 718, 779, 952
RseI CAYNNNNRTG 1 cut(s) 808
SalI GTCGAC 1 cut(s) 605
SapI GCTCTTC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 453, 920
Sau3AI GATC 2 cut(s) 87, 800
Sau96I GGNCC 3 cut(s) 520, 686, 836
ScaI AGTACT 1 cut(s) 719
SchI GAGTC 2 cut(s) 232, 620
ScrFI CCNGG 1 cut(s) 798
SduI GDGCHC 1 cut(s) 220
SfaNI GCATC 2 cut(s) 676, 882
SfcI CTRYAG 2 cut(s) 70, 848
Sfr274I CTCGAG 1 cut(s) 841
SfuI TTCGAA 1 cut(s) 422
SgrAI CRCCGGYG 1 cut(s) 818
SinI GGWCC 2 cut(s) 520, 836
SlaI CTCGAG 1 cut(s) 841
SmiMI CAYNNNNRTG 1 cut(s) 808
SmlI CTYRAG 2 cut(s) 742, 841
SmoI CTYRAG 2 cut(s) 742, 841
SpeI ACTAGT 1 cut(s) 155
Sse9I AATT 3 cut(s) 92, 164, 921
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 7 cut(s) 524, 552, 596, 680, 749, 794, 941
SspMI CTAG 3 cut(s) 156, 627, 1087
StuI AGGCCT 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 796
TaaI ACNGT 1 cut(s) 783
TaiI ACGT 1 cut(s) 906
TaqI TCGA 6 cut(s) 105, 327, 422, 513, 606, 842
TasI AATT 3 cut(s) 92, 164, 921
TatI WGTACW 2 cut(s) 717, 951
TauI GCSGC 1 cut(s) 943
TfiI GAWTC 1 cut(s) 1059
Tru1I TTAA 2 cut(s) 453, 920
Tru9I TTAA 2 cut(s) 453, 920
TscAI CASTG 4 cut(s) 55, 409, 857, 1017
TseFI GTSAC 2 cut(s) 109, 485
Tsp45I GTSAC 2 cut(s) 109, 485
TspDTI ATGAA 5 cut(s) 159, 429, 516, 950, 995
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 4 cut(s) 55, 409, 857, 1017
VpaK11BI GGWCC 2 cut(s) 520, 836
XagI CCTNNNNNAGG 1 cut(s) 50
XapI RAATTY 1 cut(s) 92
XhoI CTCGAG 1 cut(s) 841
XmiI GTMKAC 1 cut(s) 606
XspI CTAG 3 cut(s) 156, 627, 1087
ZrmI AGTACT 1 cut(s) 719
Zsp2I ATGCAT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.