Rh5BG096900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0015571
Physical Location & Seq
Forward (+)
8779413 .. 8785584
6172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG096900.1

Sequence Viewer

Length: 510 bp
ATGCATCGGCCTGTCGCGGGGGAGCCATCATTACCCTCAAGTTCTCTACAAGAGTACTGGTGGAACTACCCACCAAACTCAAGTCCGCAATTGGCCTTTGTGGGCGAGCCATTAGTACCGTTAGCCCCCCCGTCATGCGGGTGGGCCAGGTACGAGAACCACTCACAAATCCCAAGTCTGCCGTGGCCTGTTGTGGCCGAGCCACCGAGACCGTTACCCCCCACACATGGCCAGTGGATCATAGTCGAAAACCAACCGCAAACCTCAAGCCTACAATTGCCTGTCCCCGCTGGGCCATGGACACCCATTTTTTCGCCGCCGCCACCTTCAAGACCTCGAGGGAATGATAATCTAATGATCATGTTCACTCAGAATATAAATTACATGGGTCAGATGTTAATTAATAAGTTCATAAAATATATACTTCGCCATAACAAACATCAAAACATTGGTAAGAAGTGCATTTTAGATCTCCTAATCTATCTAGGATTAGCTAACAGTTTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

19.05

Weight (kDa)

9.23

Isoelectric Point (pI)

76.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 336
AccII CGCG 1 cut(s) 17
AciI CCGC 7 cut(s) 17, 86, 138, 257, 288, 317, 320
AclWI GGATC 1 cut(s) 245
AcoI YGGCCR 2 cut(s) 195, 229
AfaI GTAC 3 cut(s) 56, 117, 152
AfiI CCNNNNNNNGG 3 cut(s) 17, 137, 227
AgsI TTSAA 1 cut(s) 330
AjnI CCWGG 1 cut(s) 146
AluBI AGCT 1 cut(s) 494
AluI AGCT 1 cut(s) 494
Alw26I GTCTC 1 cut(s) 202
AlwI GGATC 1 cut(s) 245
Ama87I CYCGRG 1 cut(s) 336
AoxI GGCC 7 cut(s) 8, 93, 144, 185, 195, 229, 293
ArsI GACNNNNNNTTYG 2 cut(s) 160, 192
AseI ATTAAT 1 cut(s) 402
AspS9I GGNCC 2 cut(s) 144, 293
AvaI CYCGRG 1 cut(s) 336
BalI TGGCCA 1 cut(s) 231
BccI CCATC 1 cut(s) 34
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 1 cut(s) 148
BclI TGATCA 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 202
BfaI CTAG 1 cut(s) 485
BglII AGATCT 1 cut(s) 469
BisI GCNGC 2 cut(s) 317, 320
BlsI GCNGC 2 cut(s) 318, 321
BmcAI AGTACT 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 336
BmgT120I GGNCC 2 cut(s) 144, 293
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 148
BmsI GCATC 1 cut(s) 13
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BpuEI CTTGAG 3 cut(s) 22, 64, 250
BsaI GGTCTC 1 cut(s) 202
BsaJI CCNNGG 2 cut(s) 182, 296
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 137, 227
Bse1I ACTGG 2 cut(s) 62, 232
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 2 cut(s) 182, 296
BseLI CCNNNNNNNGG 3 cut(s) 17, 137, 227
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 2 cut(s) 62, 232
BseYI CCCAGC 1 cut(s) 290
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 7 cut(s) 10, 95, 146, 187, 197, 231, 295
BsiHKCI CYCGRG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 3 cut(s) 17, 137, 227
BsmAI GTCTC 1 cut(s) 202
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 7 cut(s) 10, 95, 146, 187, 197, 231, 295
Bso31I GGTCTC 1 cut(s) 202
BsoBI CYCGRG 1 cut(s) 336
Bsp143I GATC 3 cut(s) 237, 357, 469
Bsp19I CCATGG 1 cut(s) 296
BspACI CCGC 7 cut(s) 17, 86, 138, 257, 288, 317, 320
BspANI GGCC 7 cut(s) 10, 95, 146, 187, 197, 231, 295
BspCNI CTCAG 1 cut(s) 382
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 1 cut(s) 24
BspPI GGATC 1 cut(s) 245
BspTNI GGTCTC 1 cut(s) 202
BsrI ACTGG 2 cut(s) 62, 232
BssECI CCNNGG 2 cut(s) 182, 296
BssMI GATC 3 cut(s) 237, 357, 469
BssT1I CCWWGG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 148
Bst4CI ACNGT 3 cut(s) 120, 213, 500
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 1 cut(s) 369
BstDSI CCRYGG 2 cut(s) 182, 296
BstFNI CGCG 1 cut(s) 17
BstKTI GATC 3 cut(s) 240, 360, 472
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 3 cut(s) 237, 357, 469
BstNI CCWGG 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 146
BstUI CGCG 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BsuRI GGCC 7 cut(s) 10, 95, 146, 187, 197, 231, 295
BtgI CCRYGG 2 cut(s) 182, 296
BtsIMutI CAGTG 1 cut(s) 239
Cac8I GCNNGC 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 144, 293
Csp6I GTAC 3 cut(s) 55, 116, 151
CviAII CATG 5 cut(s) 135, 227, 297, 361, 385
CviQI GTAC 3 cut(s) 55, 116, 151
DdeI CTNAG 1 cut(s) 369
DpnI GATC 3 cut(s) 239, 359, 471
DpnII GATC 3 cut(s) 237, 357, 469
DraI TTTAAA 1 cut(s) 504
EaeI YGGCCR 2 cut(s) 195, 229
Eco130I CCWWGG 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 336
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 296
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 296
FaeI CATG 5 cut(s) 138, 230, 300, 364, 388
FaqI GGGAC 1 cut(s) 269
FatI CATG 5 cut(s) 134, 226, 296, 360, 384
FauI CCCGC 3 cut(s) 10, 131, 295
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 317, 320
Fsp4HI GCNGC 2 cut(s) 317, 320
FspBI CTAG 1 cut(s) 485
GluI GCNGC 2 cut(s) 317, 320
GsaI CCCAGC 1 cut(s) 294
HaeIII GGCC 7 cut(s) 10, 95, 146, 187, 197, 231, 295
Hin1II CATG 5 cut(s) 138, 230, 300, 364, 388
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 2 cut(s) 372, 393
Hpy188III TCNNGA 1 cut(s) 330
Hpy8I GTNNAC 1 cut(s) 366
HpyAV CCTTC 1 cut(s) 336
HpyCH4III ACNGT 3 cut(s) 120, 213, 500
HpyCH4V TGCA 2 cut(s) 4, 462
HpyF3I CTNAG 1 cut(s) 369
Hsp92II CATG 5 cut(s) 138, 230, 300, 364, 388
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 3 cut(s) 237, 357, 469
LmnI GCTCC 1 cut(s) 22
LpnPI CCDG 8 cut(s) 24, 43, 133, 160, 201, 245, 276, 294
LweI GCATC 1 cut(s) 13
MaeI CTAG 1 cut(s) 485
MaeIII GTNAC 1 cut(s) 213
MalI GATC 3 cut(s) 239, 359, 471
MboI GATC 3 cut(s) 237, 357, 469
MfeI CAATTG 2 cut(s) 89, 275
MflI RGATCY 1 cut(s) 469
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 4 cut(s) 89, 275, 379, 399
MluNI TGGCCA 1 cut(s) 231
MnlI CCTC 4 cut(s) 46, 274, 332, 345
Mox20I TGGCCA 1 cut(s) 231
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 231
MseI TTAA 3 cut(s) 398, 402, 503
MslI CAYNNNNRTG 1 cut(s) 139
Msp20I TGGCCA 1 cut(s) 231
MspA1I CMGCKG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 148
MunI CAATTG 2 cut(s) 89, 275
MvaI CCWGG 1 cut(s) 148
MvnI CGCG 1 cut(s) 17
NcoI CCATGG 1 cut(s) 296
NdeII GATC 3 cut(s) 237, 357, 469
NlaIII CATG 5 cut(s) 138, 230, 300, 364, 388
NlaIV GGNNCC 1 cut(s) 24
NmeAIII GCCGAG 1 cut(s) 223
NsiI ATGCAT 1 cut(s) 6
PacI TTAATTAA 1 cut(s) 402
PaeR7I CTCGAG 1 cut(s) 336
PkrI GCNGC 2 cut(s) 318, 321
PshBI ATTAAT 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 146
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 24
PspPI GGNCC 2 cut(s) 144, 293
PspXI VCTCGAGB 1 cut(s) 336
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 3 cut(s) 56, 117, 152
RsaNI GTAC 3 cut(s) 55, 116, 151
RseI CAYNNNNRTG 1 cut(s) 139
SaqAI TTAA 3 cut(s) 398, 402, 503
SatI GCNGC 2 cut(s) 317, 320
Sau3AI GATC 3 cut(s) 237, 357, 469
Sau96I GGNCC 2 cut(s) 144, 293
ScaI AGTACT 1 cut(s) 56
ScrFI CCNGG 1 cut(s) 148
SetI ASST 5 cut(s) 152, 266, 328, 337, 496
SfaNI GCATC 1 cut(s) 13
Sfr274I CTCGAG 1 cut(s) 336
SlaI CTCGAG 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 139
SmlI CTYRAG 4 cut(s) 37, 79, 265, 336
SmoI CTYRAG 4 cut(s) 37, 79, 265, 336
Sse9I AATT 4 cut(s) 89, 275, 379, 399
SsiI CCGC 7 cut(s) 17, 86, 138, 257, 288, 317, 320
SspMI CTAG 1 cut(s) 485
StyD4I CCNGG 1 cut(s) 146
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 3 cut(s) 120, 213, 500
TaqI TCGA 2 cut(s) 246, 337
TasI AATT 4 cut(s) 89, 275, 379, 399
TatI WGTACW 1 cut(s) 54
TauI GCSGC 2 cut(s) 319, 322
Tru1I TTAA 3 cut(s) 398, 402, 503
Tru9I TTAA 3 cut(s) 398, 402, 503
TscAI CASTG 1 cut(s) 239
TspDTI ATGAA 1 cut(s) 400
TspRI CASTG 1 cut(s) 239
VspI ATTAAT 1 cut(s) 402
XcmI CCANNNNNNNNNTGG 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 336
XspI CTAG 1 cut(s) 485
ZrmI AGTACT 1 cut(s) 56
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.