Rroxscaffold_1G00063590

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015571
Physical Location & Seq
Reverse (-)
85422190 .. 85424944
2755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063590.1

Sequence Viewer

Length: 1266 bp
ATGAATCTGCTAGTTTCACTCTCCCTAGCTAATCCTATAGAGAGGATAGAGTCGGAGGTCTCAATGGGTACACTGAGAAGCGAAGTGCGGCAACCAGAAACCCTCACTGAGGTCTCAATTCCAACCTGCAGCGACCTGTTCCCGATCACATTCCAGCTCTTCGAGGTCACCAGAAGCAGCAGTCGGACAGAAGAAGAAGCTGAAAATGCTACTAGTGAGGAATTAGAAGAAGCAAAGCTTGTAGCCGAAAGACAAACTAAACCACTCTCTCGTGCCCAGCTATTGTGTTTGAGGACTCATGACACATGGGTAATCTGCACAGGGACTCTTTCCAGCATGGGCGTCCCACAAGATGAGCAGCCTGCTATTGTTGCGAAGATATTCGAGGCCTTGAAAGTCGCCGTCCCCTTCCGGCCTATCTGCGTGGTCATCAAGGACATTGTGACCGTGCAGGAGCGCAGTGGTGGGAATGAAAGTTCGAACAAAGTGAGATTTGATAGCTTGGAAGTTAAGGAAGAGGAACAGGAGGAGGAGGAGGAGGAGGATAACTGTGCTATTTGTTTGGAGAACGTTTCTCACTTTGATAATGAGTCTGCTACTCGCTTACTTCCCTGCTCCCACTTATTTCATACAGATTGCATAACGCCGTGGCTGGATACTAATAACTTCTGTCCCCTGTGCCGTCAGTCGGTGGATGTTGCGGAGGTGGTCGACGAGTCATCAAGACCCTCAAGCCCCAGACCAGACGAAAACAACCAACACAATGATATGCATCGGCCTGTCGCGGGGGGGCCATCATTACCATCAAGTTCTCTACAAGAGTACTGGTGGAACTACCCACCAAACTCAAGTCCGCAGTTGGCCTTTGTGGGCGAGCCATTAGTACCGTTAGGCGGGTGGGCCAGGTACGAGAACCACCCACAAATCCCATGTCTGCCGTGGCCTGTTGTGGCCGAGCCACCGAGACCGTTACCCCCCACACATGGCCAGTGGGTCATAGTCGAAAACCACCCGCAAACCTCAAGCCTACAATTGCCTGTCCCCGCTGGGCCATGGACACCCATTATTTCGCCGCCACCCCCAAGACCTCGAGGGAATGATAATCTAATGATCACGTTCACTGTGTGTTTAGTATTTTTGTGCGTTTGTATACTTATGCCGCCAATGCAGTCACTTGGGGGGTTCTTTGATGGAGTGAAGAAAAACGTTATGGTTGTTACTGTTGTTTTGGCGATCATCTTTTTGCTAACTGGAGGCAGTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

421

Amino Acids

46.72

Weight (kDa)

4.69

Isoelectric Point (pI)

64.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 181 - 232 1e-05 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 182 - 228 5e-13 Ring finger domain
zf-rbx1 PF12678 182 - 228 5.2e-08 RING-H2 zinc finger domain
zf-RING_11 PF17123 183 - 214 6.7e-09 RING-like zinc finger
zf-C3HC4 PF00097 184 - 227 3.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 184 - 227 2.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 184 - 225 1e-05 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1089
Acc36I ACCTGC 1 cut(s) 134
AccI GTMKAC 2 cut(s) 711, 1150
AccII CGCG 1 cut(s) 785
AciI CCGC 9 cut(s) 88, 701, 785, 854, 894, 1013, 1044, 1073, 1160
AclI AACGTT 2 cut(s) 570, 1206
AcoI YGGCCR 2 cut(s) 951, 985
AcyI GRCGYC 1 cut(s) 342
AdeI CACNNNGTG 1 cut(s) 1125
AfaI GTAC 4 cut(s) 70, 824, 885, 908
AfiI CCNNNNNNNGG 5 cut(s) 109, 688, 785, 893, 983
AgsI TTSAA 1 cut(s) 394
AhlI ACTAGT 1 cut(s) 212
AjnI CCWGG 1 cut(s) 902
AloI GAACNNNNNNTCC 2 cut(s) 460, 492
AluBI AGCT 6 cut(s) 29, 157, 200, 238, 280, 501
AluI AGCT 6 cut(s) 29, 157, 200, 238, 280, 501
Alw26I GTCTC 3 cut(s) 64, 118, 958
Ama87I CYCGRG 1 cut(s) 1089
ApeKI GCWGC 3 cut(s) 129, 177, 358
ArsI GACNNNNNNTTYG 2 cut(s) 916, 948
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 3 cut(s) 791, 900, 1049
AsuHPI GGTGA 1 cut(s) 160
AsuII TTCGAA 1 cut(s) 479
AvaI CYCGRG 1 cut(s) 1089
BaeGI GKGCMC 1 cut(s) 277
BalI TGGCCA 1 cut(s) 987
BauI CACGAG 1 cut(s) 270
BbvI GCAGC 3 cut(s) 141, 189, 370
BccI CCATC 3 cut(s) 802, 811, 1184
BceAI ACGGC 4 cut(s) 386, 631, 666, 922
BciT130I CCWGG 1 cut(s) 904
BciVI GTATCC 1 cut(s) 649
BclI TGATCA 1 cut(s) 1110
BcoDI GTCTC 3 cut(s) 64, 118, 958
BcuI ACTAGT 1 cut(s) 212
BfaI CTAG 3 cut(s) 11, 26, 213
BfmI CTRYAG 2 cut(s) 36, 127
BfuAI ACCTGC 1 cut(s) 134
BfuI GTATCC 1 cut(s) 649
BisI GCNGC 6 cut(s) 89, 130, 178, 359, 1073, 1160
BlsI GCNGC 6 cut(s) 90, 131, 179, 360, 1074, 1161
BmcAI AGTACT 1 cut(s) 824
Bme1390I CCNGG 1 cut(s) 904
BmeT110I CYCGRG 1 cut(s) 1089
BmgT120I GGNCC 3 cut(s) 791, 900, 1049
BmiI GGNNCC 1 cut(s) 792
BmrFI CCNGG 1 cut(s) 904
BmsI GCATC 1 cut(s) 781
Bpu14I TTCGAA 1 cut(s) 479
BpuEI CTTGAG 3 cut(s) 715, 832, 1006
BsaBI GATNNNNATC 1 cut(s) 771
BsaHI GRCGYC 1 cut(s) 342
BsaI GGTCTC 3 cut(s) 64, 118, 958
BsaJI CCNNGG 3 cut(s) 647, 938, 1052
BsaXI ACNNNNNCTCC 1 cut(s) 1245
Bsc4I CCNNNNNNNGG 5 cut(s) 109, 688, 785, 893, 983
Bse1I ACTGG 3 cut(s) 830, 988, 1255
Bse8I GATNNNNATC 1 cut(s) 771
BseBI CCWGG 1 cut(s) 904
BseDI CCNNGG 3 cut(s) 647, 938, 1052
BseGI GGATG 1 cut(s) 700
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 5 cut(s) 109, 688, 785, 893, 983
BseMII CTCAG 2 cut(s) 65, 99
BseNI ACTGG 3 cut(s) 830, 988, 1255
BseRI GAGGAG 5 cut(s) 542, 545, 548, 551, 554
BseSI GKGCMC 1 cut(s) 277
BseXI GCAGC 3 cut(s) 141, 189, 370
BseYI CCCAGC 2 cut(s) 276, 1046
BsgI GTGCAG 2 cut(s) 301, 470
Bsh1236I CGCG 1 cut(s) 785
BsiHKCI CYCGRG 1 cut(s) 1089
BsiSI CCGG 1 cut(s) 412
BslFI GGGAC 5 cut(s) 329, 337, 389, 657, 1025
BslI CCNNNNNNNGG 5 cut(s) 109, 688, 785, 893, 983
BsmAI GTCTC 3 cut(s) 64, 118, 958
BsmFI GGGAC 5 cut(s) 329, 337, 389, 657, 1025
Bso31I GGTCTC 3 cut(s) 64, 118, 958
BsoBI CYCGRG 1 cut(s) 1089
Bsp119I TTCGAA 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 277
Bsp143I GATC 3 cut(s) 144, 1110, 1233
Bsp19I CCATGG 1 cut(s) 1052
BspACI CCGC 9 cut(s) 88, 701, 785, 854, 894, 1013, 1044, 1073, 1160
BspCNI CTCAG 2 cut(s) 66, 100
BspFNI CGCG 1 cut(s) 785
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 792
BspMAI CTGCAG 1 cut(s) 131
BspMI ACCTGC 1 cut(s) 134
BspQI GCTCTTC 1 cut(s) 164
BspT104I TTCGAA 1 cut(s) 479
BspTNI GGTCTC 3 cut(s) 64, 118, 958
BsrI ACTGG 3 cut(s) 830, 988, 1255
BssECI CCNNGG 3 cut(s) 647, 938, 1052
BssMI GATC 3 cut(s) 144, 1110, 1233
BssNAI GTATAC 1 cut(s) 1151
BssNI GRCGYC 1 cut(s) 342
BssSI CACGAG 1 cut(s) 270
BssT1I CCWWGG 1 cut(s) 1052
Bst1107I GTATAC 1 cut(s) 1151
Bst2BI CACGAG 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 904
Bst4CI ACNGT 6 cut(s) 448, 551, 888, 969, 1123, 1222
Bst6I CTCTTC 2 cut(s) 164, 510
BstACI GRCGYC 1 cut(s) 342
BstBI TTCGAA 1 cut(s) 479
BstC8I GCNNGC 2 cut(s) 363, 875
BstDEI CTNAG 2 cut(s) 74, 108
BstDSI CCRYGG 3 cut(s) 647, 938, 1052
BstEII GGTNACC 1 cut(s) 166
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 1 cut(s) 700
BstFNI CGCG 1 cut(s) 785
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 3 cut(s) 147, 1113, 1236
BstMAI GTCTC 3 cut(s) 64, 118, 958
BstMBI GATC 3 cut(s) 144, 1110, 1233
BstMWI GCNNNNNNNGC 3 cut(s) 206, 371, 1165
BstNI CCWGG 1 cut(s) 904
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 902
BstSFI CTRYAG 2 cut(s) 36, 127
BstSLI GKGCMC 1 cut(s) 277
BstUI CGCG 1 cut(s) 785
BstV1I GCAGC 3 cut(s) 141, 189, 370
BstZ17I GTATAC 1 cut(s) 1151
BsuI GTATCC 1 cut(s) 649
BtgI CCRYGG 3 cut(s) 647, 938, 1052
BtsCI GGATG 1 cut(s) 700
BtsI GCAGTG 2 cut(s) 466, 1264
BtsIMutI CAGTG 6 cut(s) 71, 105, 466, 995, 1119, 1264
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 2 cut(s) 363, 875
CciI TCATGA 1 cut(s) 298
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 3 cut(s) 791, 900, 1049
CseI GACGC 1 cut(s) 331
Csp6I GTAC 4 cut(s) 69, 823, 884, 907
CviAII CATG 6 cut(s) 299, 306, 337, 930, 983, 1053
CviQI GTAC 4 cut(s) 69, 823, 884, 907
DdeI CTNAG 2 cut(s) 74, 108
DpnI GATC 3 cut(s) 146, 1112, 1235
DpnII GATC 3 cut(s) 144, 1110, 1233
DraIII CACNNNGTG 1 cut(s) 1125
EaeI YGGCCR 2 cut(s) 951, 985
Eam1104I CTCTTC 2 cut(s) 164, 510
EarI CTCTTC 2 cut(s) 164, 510
Eco130I CCWWGG 1 cut(s) 1052
Eco147I AGGCCT 1 cut(s) 389
Eco31I GGTCTC 3 cut(s) 64, 118, 958
Eco88I CYCGRG 1 cut(s) 1089
Eco91I GGTNACC 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 902
EcoT14I CCWWGG 1 cut(s) 1052
EcoT22I ATGCAT 1 cut(s) 774
ErhI CCWWGG 1 cut(s) 1052
FaeI CATG 6 cut(s) 302, 309, 340, 933, 986, 1056
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FaqI GGGAC 5 cut(s) 329, 337, 389, 657, 1025
FatI CATG 6 cut(s) 298, 305, 336, 929, 982, 1052
FauI CCCGC 4 cut(s) 778, 887, 1020, 1051
FbaI TGATCA 1 cut(s) 1110
FblI GTMKAC 2 cut(s) 711, 1150
Fnu4HI GCNGC 6 cut(s) 89, 130, 178, 359, 1073, 1160
FokI GGATG 1 cut(s) 707
Fsp4HI GCNGC 6 cut(s) 89, 130, 178, 359, 1073, 1160
FspBI CTAG 3 cut(s) 11, 26, 213
GlaI GCGC 1 cut(s) 458
GluI GCNGC 6 cut(s) 89, 130, 178, 359, 1073, 1160
GsaI CCCAGC 2 cut(s) 280, 1050
HapII CCGG 1 cut(s) 412
HgaI GACGC 1 cut(s) 331
HhaI GCGC 1 cut(s) 459
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 6 cut(s) 302, 309, 340, 933, 986, 1056
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 6 cut(s) 4, 50, 295, 325, 590, 716
HpaII CCGG 1 cut(s) 412
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 4 cut(s) 71, 712, 1119, 1151
Hpy188I TCNGA 2 cut(s) 55, 186
Hpy188III TCNNGA 3 cut(s) 142, 299, 723
Hpy8I GTNNAC 4 cut(s) 71, 712, 1119, 1151
Hpy99I CGWCG 1 cut(s) 716
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 6 cut(s) 448, 551, 888, 969, 1123, 1222
HpyCH4IV ACGT 3 cut(s) 570, 1115, 1206
HpyCH4V TGCA 6 cut(s) 129, 318, 451, 639, 772, 1168
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 371, 1165
HpyF3I CTNAG 2 cut(s) 74, 108
HpySE526I ACGT 3 cut(s) 570, 1115, 1206
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 6 cut(s) 302, 309, 340, 933, 986, 1056
HspAI GCGC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 1110
Kzo9I GATC 3 cut(s) 144, 1110, 1233
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 454, 620
Lsp1109I GCAGC 3 cut(s) 141, 189, 370
LweI GCATC 1 cut(s) 781
MaeI CTAG 3 cut(s) 11, 26, 213
MaeII ACGT 3 cut(s) 570, 1115, 1206
MaeIII GTNAC 5 cut(s) 166, 442, 969, 1170, 1216
MalI GATC 3 cut(s) 146, 1112, 1235
MboI GATC 3 cut(s) 144, 1110, 1233
MboII GAAGA 7 cut(s) 151, 203, 206, 239, 388, 527, 1210
MfeI CAATTG 1 cut(s) 1031
MhlI GDGCHC 1 cut(s) 277
MlsI TGGCCA 1 cut(s) 987
MluCI AATT 3 cut(s) 117, 221, 1031
MluNI TGGCCA 1 cut(s) 987
MlyI GAGTC 5 cut(s) 59, 289, 319, 599, 725
MmeI TCCRAC 3 cut(s) 33, 146, 164
Mox20I TGGCCA 1 cut(s) 987
Mph1103I ATGCAT 1 cut(s) 774
MroXI GAANNNNTTC 1 cut(s) 380
MscI TGGCCA 1 cut(s) 987
MseI TTAA 1 cut(s) 510
Msp20I TGGCCA 1 cut(s) 987
MspA1I CMGCKG 1 cut(s) 1046
MspI CCGG 1 cut(s) 412
MspR9I CCNGG 1 cut(s) 904
MunI CAATTG 1 cut(s) 1031
MvaI CCWGG 1 cut(s) 904
MvnI CGCG 1 cut(s) 785
MwoI GCNNNNNNNGC 3 cut(s) 206, 371, 1165
NcoI CCATGG 1 cut(s) 1052
NdeII GATC 3 cut(s) 144, 1110, 1233
NlaIII CATG 6 cut(s) 302, 309, 340, 933, 986, 1056
NlaIV GGNNCC 1 cut(s) 792
NmeAIII GCCGAG 1 cut(s) 979
NmuCI GTSAC 3 cut(s) 166, 442, 1170
NsiI ATGCAT 1 cut(s) 774
NspV TTCGAA 1 cut(s) 479
PaeR7I CTCGAG 1 cut(s) 1089
PagI TCATGA 1 cut(s) 298
PceI AGGCCT 1 cut(s) 389
PciSI GCTCTTC 1 cut(s) 164
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 6 cut(s) 90, 131, 179, 360, 1074, 1161
PleI GAGTC 5 cut(s) 58, 289, 319, 598, 724
PpsI GAGTC 5 cut(s) 58, 289, 319, 598, 724
Psp1406I AACGTT 2 cut(s) 570, 1206
Psp6I CCWGG 1 cut(s) 902
PspEI GGTNACC 1 cut(s) 166
PspFI CCCAGC 2 cut(s) 276, 1046
PspGI CCWGG 1 cut(s) 902
PspN4I GGNNCC 1 cut(s) 792
PspPI GGNCC 3 cut(s) 791, 900, 1049
PspXI VCTCGAGB 1 cut(s) 1089
PstI CTGCAG 1 cut(s) 131
RsaI GTAC 4 cut(s) 70, 824, 885, 908
RsaNI GTAC 4 cut(s) 69, 823, 884, 907
SalI GTCGAC 1 cut(s) 710
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 6 cut(s) 89, 130, 178, 359, 1073, 1160
Sau3AI GATC 3 cut(s) 144, 1110, 1233
Sau96I GGNCC 3 cut(s) 791, 900, 1049
ScaI AGTACT 1 cut(s) 824
SchI GAGTC 5 cut(s) 59, 289, 319, 599, 725
ScrFI CCNGG 1 cut(s) 904
SduI GDGCHC 1 cut(s) 277
SfaNI GCATC 1 cut(s) 781
SfcI CTRYAG 2 cut(s) 36, 127
Sfr274I CTCGAG 1 cut(s) 1089
SfuI TTCGAA 1 cut(s) 479
SlaI CTCGAG 1 cut(s) 1089
SmlI CTYRAG 4 cut(s) 730, 847, 1021, 1089
SmoI CTYRAG 4 cut(s) 730, 847, 1021, 1089
SpeI ACTAGT 1 cut(s) 212
Sse9I AATT 3 cut(s) 117, 221, 1031
SseBI AGGCCT 1 cut(s) 389
SsiI CCGC 9 cut(s) 88, 701, 785, 854, 894, 1013, 1044, 1073, 1160
SspMI CTAG 3 cut(s) 11, 26, 213
StuI AGGCCT 1 cut(s) 389
StyD4I CCNGG 1 cut(s) 902
StyI CCWWGG 1 cut(s) 1052
TaaI ACNGT 6 cut(s) 448, 551, 888, 969, 1123, 1222
TaiI ACGT 3 cut(s) 573, 1118, 1209
TaqI TCGA 6 cut(s) 162, 384, 479, 711, 1002, 1090
TasI AATT 3 cut(s) 117, 221, 1031
TatI WGTACW 1 cut(s) 822
TauI GCSGC 3 cut(s) 91, 1075, 1162
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TscAI CASTG 6 cut(s) 78, 112, 466, 995, 1126, 1264
TseFI GTSAC 3 cut(s) 166, 442, 1170
TseI GCWGC 3 cut(s) 129, 177, 358
Tsp45I GTSAC 3 cut(s) 166, 442, 1170
TspDTI ATGAA 3 cut(s) 17, 486, 617
TspRI CASTG 6 cut(s) 78, 112, 466, 995, 1126, 1264
XagI CCTNNNNNAGG 1 cut(s) 107
XcmI CCANNNNNNNNNTGG 1 cut(s) 936
XhoI CTCGAG 1 cut(s) 1089
XmiI GTMKAC 2 cut(s) 711, 1150
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 3 cut(s) 11, 26, 213
ZrmI AGTACT 1 cut(s) 824
Zsp2I ATGCAT 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.