RLG00000033478

Proton pump-interactor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008759
Physical Location & Seq
Forward (+)
27853768 .. 27858087
4320 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033478

Sequence Viewer

Length: 1176 bp
ATGATTAAGGCTCTGAGATCTGACAACCAGCAAGTTAGAAAAATTGCGGATGAGAAATTTAAGGAGATTGAGCCTCTGCAGAAGGATCTCAAAAAGCTCCGCACTGCAGATCATGCCGGTCGCAATGGTGGTTTGTGCTCATCTGAGGGAGAGCTTGATGCTCGGATCAAAGGCTTGCAGTATATCATACAACATGAGAGCATTCGGTTGTCTGAGGAGAAGAAAATCCTTAAAGAGATCAAACAACTTGAAAACACACGTGGAGAAATTCAGGAATCAGTGGTTCATATGGACAAAGAGGCCCTTCAAGACCTGGTTAAACTTATAGATGGTGATTTGGAGGGAGTAAGGAAAGGGCAACAAGCAGTTAGGTCCAAAATTCAGCAACTTGATAATGCAATTAAGGCCGTAGATAAGCAAATTTCATCAATACGGGATGAGCTGAAGATTGTAACTGATAAGAGGGATCAAGCACAACAGAGCATTTTTACATTTAGGCAGCGGCTTGATCAGCAAAATGCCTCCTTTTACCTAAGCCGTAATATCTTGAACAAAGCAAGAGGTTTGGCTATCAAGAATGATGTTAAAGCACTTGAAGAGCTTACTCATGCAGATAGGTGGAGAAATTTGTCCCAGTGGAATAGTAAAAAGGATTTTAGGGATGAATATGAGAAAAGAATTTTGTCGTCTCTGGGTGGTCGGCAATTGAGTACGGATGGACGAATAAGAAACCCAGATGAAAAGCCATTGGTGGTTCATGAACTAATACAAAAACAAGTCAAGGAAGATGGCAGATCTGCTCCATTAGATAGTTTGCCTGTGCAGAAGGTTCGGAAAGAAGCCAAAAGTAAAGCAGTGCCTCCAAAATCCACTGTAGACGAAGACAAGGAGATATATGGGCTCGAGAAGCCGAAAAAGGACAGTAAAGAAAGAAAAGTTGATGAAGCAAAGTTGAAGGAGATGAAAAGAGAGGAGGAAATTGCTAAAGCTAAGCAGGCCATGGAAAGGAAGAAGAGAAAGCTGCAGCTAAAGCAGAAATTAGAGCTCAAAAAGAAGCCGAAAAGAAGCTCTAGGAGCGTGAAAAGAAAGCAAAGAAGAGGGCTGCATTGGCAACTGAGAGCAATCCTGATGAGGCAACGGAACCAATTTTTGAAGTTGCAGAACCAGAAAAGGTAA

Protein Analysis

392

Amino Acids

45.68

Weight (kDa)

10.02

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7121 PF23435 10 - 185 1.2e-06 Family of unknown function (DUF7121)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 876
AciI CCGC 3 cut(s) 47, 100, 502
AclWI GGATC 3 cut(s) 93, 173, 474
AcsI RAATTY 6 cut(s) 56, 267, 378, 420, 625, 678
AcuI CTGAAG 1 cut(s) 464
AcvI CACGTG 1 cut(s) 260
AfaI GTAC 1 cut(s) 712
AfiI CCNNNNNNNGG 1 cut(s) 1005
AflIII ACRYGT 1 cut(s) 257
AgsI TTSAA 6 cut(s) 251, 308, 550, 596, 955, 1153
AjnI CCWGG 1 cut(s) 312
AluBI AGCT 9 cut(s) 97, 154, 442, 601, 989, 1021, 1027, 1045, 1068
AluI AGCT 9 cut(s) 97, 154, 442, 601, 989, 1021, 1027, 1045, 1068
Alw21I GWGCWC 2 cut(s) 140, 1047
Alw26I GTCTC 1 cut(s) 693
AlwI GGATC 3 cut(s) 93, 173, 474
Ama87I CYCGRG 1 cut(s) 902
AoxI GGCC 3 cut(s) 300, 405, 996
ApeKI GCWGC 4 cut(s) 499, 1021, 1024, 1102
ApoI RAATTY 6 cut(s) 56, 267, 378, 420, 625, 678
AspS9I GGNCC 2 cut(s) 301, 372
AsuHPI GGTGA 1 cut(s) 344
AvaI CYCGRG 1 cut(s) 902
AvaII GGWCC 1 cut(s) 372
BanII GRGCYC 2 cut(s) 903, 1047
BbrPI CACGTG 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 888
Bbv12I GWGCWC 2 cut(s) 140, 1047
BbvI GCAGC 4 cut(s) 511, 1008, 1036, 1089
BccI CCATC 3 cut(s) 323, 710, 782
BceAI ACGGC 2 cut(s) 392, 522
BcgI CGANNNNNNTGC 2 cut(s) 812, 846
BciT130I CCWGG 1 cut(s) 314
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 693
BfaI CTAG 1 cut(s) 1071
BfmI CTRYAG 4 cut(s) 77, 105, 873, 1022
BglII AGATCT 2 cut(s) 17, 794
BisI GCNGC 5 cut(s) 500, 503, 1022, 1025, 1103
BlpI GCTNAGC 1 cut(s) 990
BlsI GCNGC 5 cut(s) 501, 504, 1023, 1026, 1104
Bme1390I CCNGG 1 cut(s) 314
Bme18I GGWCC 1 cut(s) 372
BmeT110I CYCGRG 1 cut(s) 902
BmgT120I GGNCC 2 cut(s) 301, 372
BmiI GGNNCC 1 cut(s) 1142
BmrFI CCNGG 1 cut(s) 314
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 1 cut(s) 148
BmuI ACTGGG 1 cut(s) 628
BpiI GAAGAC 1 cut(s) 888
Bpu10I CCTNAGC 1 cut(s) 533
Bpu1102I GCTNAGC 1 cut(s) 990
BsaAI YACGTR 1 cut(s) 260
BsaJI CCNNGG 1 cut(s) 999
Bsc4I CCNNNNNNNGG 1 cut(s) 1005
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 634
Bse3DI GCAATG 1 cut(s) 130
BseBI CCWGG 1 cut(s) 314
BseDI CCNNGG 1 cut(s) 999
BseGI GGATG 4 cut(s) 55, 442, 667, 721
BseLI CCNNNNNNNGG 1 cut(s) 1005
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 4 cut(s) 5, 135, 204, 1106
BseNI ACTGG 1 cut(s) 634
BseRI GAGGAG 2 cut(s) 230, 986
BseXI GCAGC 4 cut(s) 511, 1008, 1036, 1089
BsgI GTGCAG 1 cut(s) 842
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 3 cut(s) 302, 407, 998
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 2 cut(s) 140, 1047
BsiHKCI CYCGRG 1 cut(s) 902
BsiSI CCGG 1 cut(s) 117
BslFI GGGAC 1 cut(s) 616
BslI CCNNNNNNNGG 1 cut(s) 1005
BsmAI GTCTC 1 cut(s) 693
BsmBI CGTCTC 1 cut(s) 693
BsmFI GGGAC 1 cut(s) 616
BsmI GAATGC 1 cut(s) 201
BsnI GGCC 3 cut(s) 302, 407, 998
BsoBI CYCGRG 1 cut(s) 902
Bsp1286I GDGCHC 3 cut(s) 140, 903, 1047
Bsp143I GATC 8 cut(s) 17, 85, 109, 165, 237, 466, 508, 794
Bsp1720I GCTNAGC 1 cut(s) 990
Bsp19I CCATGG 1 cut(s) 999
BspACI CCGC 3 cut(s) 47, 100, 502
BspANI GGCC 3 cut(s) 302, 407, 998
BspCNI CTCAG 4 cut(s) 6, 136, 205, 1107
BspHI TCATGA 1 cut(s) 757
BspLI GGNNCC 1 cut(s) 1142
BspMAI CTGCAG 3 cut(s) 81, 109, 1026
BspPI GGATC 3 cut(s) 93, 173, 474
BspQI GCTCTTC 1 cut(s) 591
BsrDI GCAATG 1 cut(s) 130
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 1 cut(s) 634
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 1 cut(s) 999
BssMI GATC 8 cut(s) 17, 85, 109, 165, 237, 466, 508, 794
BssT1I CCWWGG 1 cut(s) 999
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 874, 923
Bst6I CTCTTC 3 cut(s) 591, 1007, 1090
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 260
BstC8I GCNNGC 2 cut(s) 176, 996
BstDEI CTNAG 6 cut(s) 14, 144, 213, 533, 990, 1115
BstDSI CCRYGG 1 cut(s) 999
BstF5I GGATG 4 cut(s) 55, 442, 667, 721
BstKTI GATC 8 cut(s) 20, 88, 112, 168, 240, 469, 511, 797
BstMAI GTCTC 1 cut(s) 693
BstMBI GATC 8 cut(s) 17, 85, 109, 165, 237, 466, 508, 794
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 8 cut(s) 113, 404, 511, 907, 995, 1030, 1074, 1108
BstNI CCWGG 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 312
BstSFI CTRYAG 4 cut(s) 77, 105, 873, 1022
BstV1I GCAGC 4 cut(s) 511, 1008, 1036, 1089
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 3 cut(s) 17, 85, 794
BstYI RGATCY 3 cut(s) 17, 85, 794
BsuRI GGCC 3 cut(s) 302, 407, 998
BtgI CCRYGG 1 cut(s) 999
BtsCI GGATG 4 cut(s) 55, 442, 667, 721
BtsI GCAGTG 2 cut(s) 102, 861
BtsIMutI CAGTG 5 cut(s) 102, 285, 641, 861, 870
Cac8I GCNNGC 2 cut(s) 176, 996
CciI TCATGA 1 cut(s) 757
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 2 cut(s) 301, 372
CsiI ACCWGGT 1 cut(s) 312
Csp6I GTAC 1 cut(s) 711
CviAII CATG 5 cut(s) 113, 194, 608, 758, 1000
CviQI GTAC 1 cut(s) 711
DdeI CTNAG 6 cut(s) 14, 144, 213, 533, 990, 1115
DpnI GATC 8 cut(s) 19, 87, 111, 167, 239, 468, 510, 796
DpnII GATC 8 cut(s) 17, 85, 109, 165, 237, 466, 508, 794
Eam1104I CTCTTC 3 cut(s) 591, 1007, 1090
EarI CTCTTC 3 cut(s) 591, 1007, 1090
Ecl136II GAGCTC 1 cut(s) 1045
Eco130I CCWWGG 1 cut(s) 999
Eco24I GRGCYC 2 cut(s) 903, 1047
Eco47I GGWCC 1 cut(s) 372
Eco53kI GAGCTC 1 cut(s) 1045
Eco57I CTGAAG 1 cut(s) 464
Eco72I CACGTG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 902
EcoICRI GAGCTC 1 cut(s) 1045
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 312
EcoT14I CCWWGG 1 cut(s) 999
EcoT38I GRGCYC 2 cut(s) 903, 1047
ErhI CCWWGG 1 cut(s) 999
Esp3I CGTCTC 1 cut(s) 693
FaeI CATG 5 cut(s) 116, 197, 611, 761, 1003
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FaqI GGGAC 1 cut(s) 616
FatI CATG 5 cut(s) 112, 193, 607, 757, 999
FauNDI CATATG 1 cut(s) 288
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 876
Fnu4HI GCNGC 5 cut(s) 500, 503, 1022, 1025, 1103
FokI GGATG 4 cut(s) 62, 449, 674, 728
FriOI GRGCYC 2 cut(s) 903, 1047
Fsp4HI GCNGC 5 cut(s) 500, 503, 1022, 1025, 1103
FspBI CTAG 1 cut(s) 1071
GluI GCNGC 5 cut(s) 500, 503, 1022, 1025, 1103
HaeIII GGCC 3 cut(s) 302, 407, 998
HapII CCGG 1 cut(s) 117
Hin1II CATG 5 cut(s) 116, 197, 611, 761, 1003
HinfI GANTC 1 cut(s) 275
HpaII CCGG 1 cut(s) 117
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 6 cut(s) 15, 22, 145, 165, 214, 834
Hpy188III TCNNGA 7 cut(s) 272, 308, 547, 574, 758, 904, 1126
Hpy8I GTNNAC 1 cut(s) 877
HpyAV CCTTC 4 cut(s) 76, 314, 820, 949
HpyCH4III ACNGT 2 cut(s) 874, 923
HpyCH4IV ACGT 1 cut(s) 259
HpyCH4V TGCA 9 cut(s) 79, 107, 178, 398, 611, 823, 1024, 1105, 1159
HpyF10VI GCNNNNNNNGC 8 cut(s) 113, 404, 511, 907, 995, 1030, 1074, 1108
HpyF3I CTNAG 6 cut(s) 14, 144, 213, 533, 990, 1115
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 5 cut(s) 116, 197, 611, 761, 1003
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 8 cut(s) 17, 85, 109, 165, 237, 466, 508, 794
LguI GCTCTTC 1 cut(s) 591
LmnI GCTCC 3 cut(s) 102, 805, 1074
Lsp1109I GCAGC 4 cut(s) 511, 1008, 1036, 1089
LweI GCATC 1 cut(s) 148
MabI ACCWGGT 1 cut(s) 312
MaeI CTAG 1 cut(s) 1071
MaeII ACGT 1 cut(s) 259
MaeIII GTNAC 1 cut(s) 451
MalI GATC 8 cut(s) 19, 87, 111, 167, 239, 468, 510, 796
MboI GATC 8 cut(s) 17, 85, 109, 165, 237, 466, 508, 794
MboII GAAGA 8 cut(s) 232, 457, 608, 797, 893, 1021, 1024, 1107
MfeI CAATTG 1 cut(s) 704
MflI RGATCY 3 cut(s) 17, 85, 794
MhlI GDGCHC 3 cut(s) 140, 903, 1047
MseI TTAA 6 cut(s) 6, 60, 231, 318, 402, 585
MspA1I CMGCKG 1 cut(s) 502
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 314
MunI CAATTG 1 cut(s) 704
Mva1269I GAATGC 1 cut(s) 201
MvaI CCWGG 1 cut(s) 314
MwoI GCNNNNNNNGC 8 cut(s) 113, 404, 511, 907, 995, 1030, 1074, 1108
NcoI CCATGG 1 cut(s) 999
NdeI CATATG 1 cut(s) 288
NdeII GATC 8 cut(s) 17, 85, 109, 165, 237, 466, 508, 794
NlaIII CATG 5 cut(s) 116, 197, 611, 761, 1003
NlaIV GGNNCC 1 cut(s) 1142
PaeR7I CTCGAG 1 cut(s) 902
PagI TCATGA 1 cut(s) 757
PciSI GCTCTTC 1 cut(s) 591
PctI GAATGC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 5 cut(s) 501, 504, 1023, 1026, 1104
PmaCI CACGTG 1 cut(s) 260
PmlI CACGTG 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 260
Psp124BI GAGCTC 1 cut(s) 1047
Psp6I CCWGG 1 cut(s) 312
PspCI CACGTG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 1 cut(s) 1142
PspPI GGNCC 2 cut(s) 301, 372
PstI CTGCAG 3 cut(s) 81, 109, 1026
PsuI RGATCY 3 cut(s) 17, 85, 794
RsaI GTAC 1 cut(s) 712
RsaNI GTAC 1 cut(s) 711
SacI GAGCTC 1 cut(s) 1047
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 6 cut(s) 6, 60, 231, 318, 402, 585
SatI GCNGC 5 cut(s) 500, 503, 1022, 1025, 1103
Sau3AI GATC 8 cut(s) 17, 85, 109, 165, 237, 466, 508, 794
Sau96I GGNCC 2 cut(s) 301, 372
ScrFI CCNGG 1 cut(s) 314
SduI GDGCHC 3 cut(s) 140, 903, 1047
SexAI ACCWGGT 1 cut(s) 312
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 4 cut(s) 77, 105, 873, 1022
Sfr274I CTCGAG 1 cut(s) 902
SinI GGWCC 1 cut(s) 372
SlaI CTCGAG 1 cut(s) 902
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
SsiI CCGC 3 cut(s) 47, 100, 502
SspMI CTAG 1 cut(s) 1071
SstI GAGCTC 1 cut(s) 1047
StyD4I CCNGG 1 cut(s) 312
StyI CCWWGG 1 cut(s) 999
TaaI ACNGT 2 cut(s) 874, 923
TaiI ACGT 1 cut(s) 262
TaqI TCGA 1 cut(s) 903
TauI GCSGC 1 cut(s) 505
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 6 cut(s) 6, 60, 231, 318, 402, 585
Tru9I TTAA 6 cut(s) 6, 60, 231, 318, 402, 585
TscAI CASTG 5 cut(s) 109, 285, 641, 861, 877
TseI GCWGC 4 cut(s) 499, 1021, 1024, 1102
TspDTI ATGAA 8 cut(s) 275, 414, 678, 746, 753, 774, 957, 977
TspGWI ACGGA 2 cut(s) 728, 1153
TspRI CASTG 5 cut(s) 109, 285, 641, 861, 877
VpaK11BI GGWCC 1 cut(s) 372
XapI RAATTY 6 cut(s) 56, 267, 378, 420, 625, 678
XhoI CTCGAG 1 cut(s) 902
XmiI GTMKAC 1 cut(s) 876
XspI CTAG 1 cut(s) 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.