RLG00000034426

Protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010308
Physical Location & Seq
Reverse (-)
42567635 .. 42569783
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034426

Sequence Viewer

Length: 1608 bp
ATGCTCTTCTCTTCGTCTTCTTCGGTTGTGCCCTCACCCAAGGTCTCAATGGAACCAAAGCTGTTGCTTTCTTCAACCACAGCCTTACCAGCCACTTCCTTACCCTTTTTTCTTTCATACAACTCTCCTTTTCATTTGAGATCCAAACAGTCTGTGAAAACCCATCTCTCAATCTCAAGGATACAGCCGGAAAAATCAAACTTTTGGGGAAATAGTTTGGTTTTACCTGAGAGAGATGTTAGTTTGGGTGATTTGAGACATGTCCCAGTTGGACCAATACGTGCTGCTGTGAAGAGAAGGAAAGAACTTCCTTTCGACAATGTGATCCAAAGGGATAAGAAGTTGAAGTTAGTTTTGAAGATTAGAAAAATTTTAGTGAGCCAACCTGATAGAATTATGTCACTTAGGGAATTAGGTAGGTATAGAAGAGAACTGGGTCTGCAGAAAAAGAGGCGGTTTATTGCATTACTGAAGAAGTTCCCTGCTGTGTTTGAGATTGTTGAAGAAGGTGTATTTTCGTTGAAATTTAAATTAACGCCCGAAGCAGAAAGGCTTTATCTTGAAGAGTTGCAGGTTCGAAATGAGATGGAAGACTTGCTGGTCGTGAAGTTGAGGAAATTATTGATGATGTCATTGGATAAACGGATTTTGTTGGAGAAGATTGCTCATTTGAGGACTGATTTAGTGCTTCCTTTAGAATTTCGTGATACAATATGTCATCGATACCCACAGTATTTTAAAGTTGTTGCAACTGCGAGAGGTCCAGCATTAGAATTAACTCATTGGGATCCTGAGCTTGCTGTATCAGCTGCTGAGTTATCTGAAGAGGAAAATCGTGCTAGAGAATTAGAAGAGAAGGATTTGATTATAGATAGGCCTCTCAAGTTCAATAGACTAAAGTTACCAAGGGGTCTTAATCTTTCCAAGGGTGAGATGAGGAGAATATGTCTGTTTAGAGACATCCCTTATATATCCCCTTACTCTGATTTCTCGGGGCTCAGGTCAGGTACAATTGAGAAAGAGAAACATGCTTGTGCAGTTGTCCATGAGATATTGAGCCTTACTGTGGAGAAGAAAACTCTTGTAGATCACCTTACTCATTTCCGCGAGGAGTTTAGGTTCTCTCAACAGGTCAGGGGAATGCTGATAAGGCACCCTGATATGTTTTATGTCTCGTTGAAGGGAGATAGGGATTCAGTTTTCCTTAGGGAAGCATATCGTGATTCTCAATTGATAGACAAGGATCGTCTGCTGATTATAAAAGAGAAGCTTCGTTCTATTATTGCAGTTCCACGGTATCCTAGAAGGGGTGCTCCCAATAGAGAGGCAGATGGTGCTGAAGGAACCAATGAGGCACAAGATGGAACCGTTGATGAGGGAGAGGAATGGTCCGATACTGATAACTTTATGAGTGATGAAGATGATGATGATGATGTAGATGATTGGAGTGATGAAGATGAAGATACGCCCCCAGATTTCAACGAAGATGGGAAAACATTAGACAGTGAACCGAGCAAACCAATTAATCAAGAAGGCTATTCAACAGAGCATGATGAGAGGGTGCTTGTTCCAGTCTTGCCAGATGGTCGGCCAAGAGAGCGCTGGTAA

Protein Analysis

536

Amino Acids

61.98

Weight (kDa)

6.26

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 101 - 428 4.1e-106 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1259
AasI GACNNNNNNGTC 1 cut(s) 599
Acc36I ACCTGC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 1152
AccII CGCG 1 cut(s) 1107
AciI CCGC 2 cut(s) 454, 1105
AclWI GGATC 5 cut(s) 135, 319, 782, 795, 1251
AcoI YGGCCR 1 cut(s) 1589
AcsI RAATTY 3 cut(s) 369, 524, 698
AcuI CTGAAG 3 cut(s) 491, 843, 1359
AfaI GTAC 1 cut(s) 1009
AfeI AGCGCT 1 cut(s) 1601
AfiI CCNNNNNNNGG 2 cut(s) 1066, 1307
AflIII ACRYGT 1 cut(s) 259
AloI GAACNNNNNNTCC 2 cut(s) 1103, 1135
AluBI AGCT 4 cut(s) 61, 796, 809, 1270
AluI AGCT 4 cut(s) 61, 796, 809, 1270
Alw21I GWGCWC 1 cut(s) 1315
Alw26I GTCTC 4 cut(s) 49, 250, 951, 1177
AlwI GGATC 5 cut(s) 135, 319, 782, 795, 1251
AlwNI CAGNNNCTG 1 cut(s) 812
Ama87I CYCGRG 1 cut(s) 991
Aor51HI AGCGCT 1 cut(s) 1601
AoxI GGCC 2 cut(s) 875, 1589
ApeKI GCWGC 2 cut(s) 284, 809
ApoI RAATTY 3 cut(s) 369, 524, 698
AseI ATTAAT 1 cut(s) 1524
Asp700I GAANNNNTTC 1 cut(s) 476
AspLEI GCGC 1 cut(s) 1602
AspS9I GGNCC 3 cut(s) 272, 761, 1389
AsuHPI GGTGA 4 cut(s) 27, 260, 941, 1082
AsuII TTCGAA 1 cut(s) 577
AvaI CYCGRG 1 cut(s) 991
AvaII GGWCC 3 cut(s) 272, 761, 1389
AxyI CCTNAGG 1 cut(s) 1205
BaeGI GKGCMC 1 cut(s) 33
BamHI GGATCC 1 cut(s) 787
BanI GGYRCC 1 cut(s) 1152
BanII GRGCYC 1 cut(s) 999
BbsI GAAGAC 2 cut(s) 9, 597
Bbv12I GWGCWC 1 cut(s) 1315
BbvI GCAGC 2 cut(s) 271, 796
BccI CCATC 6 cut(s) 171, 580, 1325, 1355, 1481, 1577
BciVI GTATCC 2 cut(s) 174, 1308
BcoDI GTCTC 4 cut(s) 49, 250, 951, 1177
BfaI CTAG 2 cut(s) 840, 1302
BfmI CTRYAG 1 cut(s) 440
BfoI RGCGCY 1 cut(s) 1603
BfuAI ACCTGC 1 cut(s) 562
BfuI GTATCC 2 cut(s) 174, 1308
BisI GCNGC 2 cut(s) 285, 810
BlsI GCNGC 2 cut(s) 286, 811
Bme18I GGWCC 3 cut(s) 272, 761, 1389
BmeT110I CYCGRG 1 cut(s) 991
BmgT120I GGNCC 3 cut(s) 272, 761, 1389
BmiI GGNNCC 5 cut(s) 54, 789, 1154, 1345, 1366
BmrI ACTGGG 2 cut(s) 260, 443
BmuI ACTGGG 2 cut(s) 260, 443
BpiI GAAGAC 2 cut(s) 9, 597
Bpu10I CCTNAGC 2 cut(s) 792, 998
Bpu14I TTCGAA 1 cut(s) 577
BpuEI CTTGAG 2 cut(s) 160, 866
Bsa29I ATCGAT 1 cut(s) 721
BsaAI YACGTR 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 4 cut(s) 39, 905, 924, 1292
BsaXI ACNNNNNCTCC 4 cut(s) 931, 961, 1103, 1133
Bsc4I CCNNNNNNNGG 2 cut(s) 1066, 1307
Bse1I ACTGG 3 cut(s) 266, 438, 1571
Bse21I CCTNAGG 1 cut(s) 1205
BseCI ATCGAT 1 cut(s) 721
BseDI CCNNGG 4 cut(s) 39, 905, 924, 1292
BseGI GGATG 1 cut(s) 960
BseLI CCNNNNNNNGG 2 cut(s) 1066, 1307
BseMII CTCAG 4 cut(s) 219, 783, 804, 1012
BseNI ACTGG 3 cut(s) 266, 438, 1571
BseRI GAGGAG 2 cut(s) 952, 1124
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 2 cut(s) 271, 796
BsgI GTGCAG 1 cut(s) 1056
Bsh1236I CGCG 1 cut(s) 1107
BshFI GGCC 2 cut(s) 877, 1591
BshNI GGYRCC 1 cut(s) 1152
BshVI ATCGAT 1 cut(s) 721
BsiHKAI GWGCWC 1 cut(s) 1315
BsiHKCI CYCGRG 1 cut(s) 991
BsiSI CCGG 1 cut(s) 188
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 2 cut(s) 1066, 1307
BsmAI GTCTC 4 cut(s) 49, 250, 951, 1177
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 1146
BsnI GGCC 2 cut(s) 877, 1591
Bso31I GGTCTC 1 cut(s) 49
BsoBI CYCGRG 1 cut(s) 991
Bsp119I TTCGAA 1 cut(s) 577
Bsp1286I GDGCHC 3 cut(s) 33, 999, 1315
Bsp143I GATC 5 cut(s) 140, 324, 787, 1087, 1243
BspACI CCGC 2 cut(s) 454, 1105
BspANI GGCC 2 cut(s) 877, 1591
BspCNI CTCAG 4 cut(s) 220, 784, 805, 1011
BspDI ATCGAT 1 cut(s) 721
BspFNI CGCG 1 cut(s) 1107
BspLI GGNNCC 5 cut(s) 54, 789, 1154, 1345, 1366
BspMAI CTGCAG 1 cut(s) 444
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 5 cut(s) 135, 319, 782, 795, 1251
BspQI GCTCTTC 1 cut(s) 11
BspT104I TTCGAA 1 cut(s) 577
BspT107I GGYRCC 1 cut(s) 1152
BspTNI GGTCTC 1 cut(s) 49
BsrI ACTGG 3 cut(s) 266, 438, 1571
BssECI CCNNGG 4 cut(s) 39, 905, 924, 1292
BssMI GATC 5 cut(s) 140, 324, 787, 1087, 1243
BssT1I CCWWGG 3 cut(s) 39, 905, 924
Bst4CI ACNGT 6 cut(s) 150, 732, 1066, 1296, 1369, 1505
Bst6I CTCTTC 7 cut(s) 11, 16, 287, 421, 558, 819, 846
BstAPI GCANNNNNTGC 1 cut(s) 1334
BstBAI YACGTR 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 577
BstC8I GCNNGC 1 cut(s) 798
BstDEI CTNAG 6 cut(s) 228, 404, 792, 813, 998, 1205
BstDSI CCRYGG 1 cut(s) 1292
BstF5I GGATG 1 cut(s) 960
BstFNI CGCG 1 cut(s) 1107
BstH2I RGCGCY 1 cut(s) 1603
BstHHI GCGC 1 cut(s) 1602
BstKTI GATC 5 cut(s) 143, 327, 790, 1090, 1246
BstMAI GTCTC 4 cut(s) 49, 250, 951, 1177
BstMBI GATC 5 cut(s) 140, 324, 787, 1087, 1243
BstMWI GCNNNNNNNGC 5 cut(s) 89, 806, 1150, 1334, 1597
BstNSI RCATGY 2 cut(s) 263, 1031
BstSFI CTRYAG 1 cut(s) 440
BstSLI GKGCMC 1 cut(s) 33
BstUI CGCG 1 cut(s) 1107
BstV1I GCAGC 2 cut(s) 271, 796
BstV2I GAAGAC 2 cut(s) 9, 597
BstX2I RGATCY 2 cut(s) 140, 787
BstYI RGATCY 2 cut(s) 140, 787
Bsu15I ATCGAT 1 cut(s) 721
Bsu36I CCTNAGG 1 cut(s) 1205
BsuI GTATCC 2 cut(s) 174, 1308
BsuRI GGCC 2 cut(s) 877, 1591
BsuTUI ATCGAT 1 cut(s) 721
BtgI CCRYGG 1 cut(s) 1292
BtsCI GGATG 1 cut(s) 960
BtsIMutI CAGTG 1 cut(s) 1510
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 1 cut(s) 798
CaiI CAGNNNCTG 1 cut(s) 812
CfoI GCGC 1 cut(s) 1602
Cfr13I GGNCC 3 cut(s) 272, 761, 1389
ClaI ATCGAT 1 cut(s) 721
Csp6I GTAC 1 cut(s) 1008
CviAII CATG 4 cut(s) 260, 1028, 1046, 1550
CviQI GTAC 1 cut(s) 1008
DdeI CTNAG 6 cut(s) 228, 404, 792, 813, 998, 1205
DpnI GATC 5 cut(s) 142, 326, 789, 1089, 1245
DpnII GATC 5 cut(s) 140, 324, 787, 1087, 1243
DraI TTTAAA 2 cut(s) 529, 739
DrdI GACNNNNNNGTC 1 cut(s) 599
DseDI GACNNNNNNGTC 1 cut(s) 599
EaeI YGGCCR 1 cut(s) 1589
Eam1104I CTCTTC 7 cut(s) 11, 16, 287, 421, 558, 819, 846
EarI CTCTTC 7 cut(s) 11, 16, 287, 421, 558, 819, 846
Eco130I CCWWGG 3 cut(s) 39, 905, 924
Eco147I AGGCCT 1 cut(s) 877
Eco24I GRGCYC 1 cut(s) 999
Eco31I GGTCTC 1 cut(s) 49
Eco47I GGWCC 3 cut(s) 272, 761, 1389
Eco47III AGCGCT 1 cut(s) 1601
Eco57I CTGAAG 3 cut(s) 491, 843, 1359
Eco81I CCTNAGG 1 cut(s) 1205
Eco88I CYCGRG 1 cut(s) 991
EcoT14I CCWWGG 3 cut(s) 39, 905, 924
EcoT38I GRGCYC 1 cut(s) 999
ErhI CCWWGG 3 cut(s) 39, 905, 924
FaeI CATG 4 cut(s) 263, 1031, 1049, 1553
FalI AAGNNNNNCTT 2 cut(s) 1254, 1286
FaqI GGGAC 1 cut(s) 248
FatI CATG 4 cut(s) 259, 1027, 1045, 1549
Fnu4HI GCNGC 2 cut(s) 285, 810
FokI GGATG 1 cut(s) 947
FriOI GRGCYC 1 cut(s) 999
Fsp4HI GCNGC 2 cut(s) 285, 810
FspBI CTAG 2 cut(s) 840, 1302
GlaI GCGC 1 cut(s) 1601
GluI GCNGC 2 cut(s) 285, 810
HaeII RGCGCY 1 cut(s) 1603
HaeIII GGCC 2 cut(s) 877, 1591
HapII CCGG 1 cut(s) 188
HhaI GCGC 1 cut(s) 1602
Hin1II CATG 4 cut(s) 263, 1031, 1049, 1553
Hin6I GCGC 1 cut(s) 1600
HinP1I GCGC 1 cut(s) 1600
HindIII AAGCTT 1 cut(s) 1268
HinfI GANTC 2 cut(s) 1193, 1223
HpaII CCGG 1 cut(s) 188
HphI GGTGA 4 cut(s) 27, 260, 941, 1082
Hpy166II GTNNAC 1 cut(s) 1508
Hpy188I TCNGA 3 cut(s) 823, 985, 1393
Hpy188III TCNNGA 6 cut(s) 560, 604, 704, 791, 1220, 1529
Hpy8I GTNNAC 1 cut(s) 1508
HpyAV CCTTC 7 cut(s) 291, 500, 850, 1174, 1299, 1334, 1526
HpyCH4III ACNGT 6 cut(s) 150, 732, 1066, 1296, 1369, 1505
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 6 cut(s) 442, 464, 571, 749, 1037, 1286
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 806, 1150, 1334, 1597
HpyF3I CTNAG 6 cut(s) 228, 404, 792, 813, 998, 1205
HpySE526I ACGT 1 cut(s) 280
Hsp92II CATG 4 cut(s) 263, 1031, 1049, 1553
HspAI GCGC 1 cut(s) 1600
Kzo9I GATC 5 cut(s) 140, 324, 787, 1087, 1243
LguI GCTCTTC 1 cut(s) 11
LmnI GCTCC 1 cut(s) 1318
Lsp1109I GCAGC 2 cut(s) 271, 796
MaeI CTAG 2 cut(s) 840, 1302
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 2 cut(s) 399, 900
MalI GATC 5 cut(s) 142, 326, 789, 1089, 1245
MboI GATC 5 cut(s) 140, 324, 787, 1087, 1243
MfeI CAATTG 2 cut(s) 1011, 1229
MflI RGATCY 2 cut(s) 140, 787
MhlI GDGCHC 3 cut(s) 33, 999, 1315
MmeI TCCRAC 2 cut(s) 250, 633
MroXI GAANNNNTTC 1 cut(s) 476
MseI TTAA 6 cut(s) 528, 533, 738, 776, 915, 1524
MslI CAYNNNNRTG 1 cut(s) 1032
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 1 cut(s) 188
MunI CAATTG 2 cut(s) 1011, 1229
Mva1269I GAATGC 1 cut(s) 1146
MvnI CGCG 1 cut(s) 1107
MwoI GCNNNNNNNGC 5 cut(s) 89, 806, 1150, 1334, 1597
NdeII GATC 5 cut(s) 140, 324, 787, 1087, 1243
NlaIII CATG 4 cut(s) 263, 1031, 1049, 1553
NlaIV GGNNCC 5 cut(s) 54, 789, 1154, 1345, 1366
NmuCI GTSAC 1 cut(s) 399
NspI RCATGY 2 cut(s) 263, 1031
NspV TTCGAA 1 cut(s) 577
PceI AGGCCT 1 cut(s) 877
PciI ACATGT 1 cut(s) 259
PciSI GCTCTTC 1 cut(s) 11
PctI GAATGC 1 cut(s) 1146
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 2 cut(s) 1193, 1223
PkrI GCNGC 2 cut(s) 286, 811
Ppu21I YACGTR 1 cut(s) 281
PscI ACATGT 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 1524
PsiI TTATAA 1 cut(s) 1259
PspN4I GGNNCC 5 cut(s) 54, 789, 1154, 1345, 1366
PspPI GGNCC 3 cut(s) 272, 761, 1389
PstI CTGCAG 1 cut(s) 444
PstNI CAGNNNCTG 1 cut(s) 812
PsuI RGATCY 2 cut(s) 140, 787
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 1 cut(s) 1009
RsaNI GTAC 1 cut(s) 1008
RseI CAYNNNNRTG 1 cut(s) 1032
SapI GCTCTTC 1 cut(s) 11
SaqAI TTAA 6 cut(s) 528, 533, 738, 776, 915, 1524
SatI GCNGC 2 cut(s) 285, 810
Sau3AI GATC 5 cut(s) 140, 324, 787, 1087, 1243
Sau96I GGNCC 3 cut(s) 272, 761, 1389
SduI GDGCHC 3 cut(s) 33, 999, 1315
SfcI CTRYAG 1 cut(s) 440
SfuI TTCGAA 1 cut(s) 577
SinI GGWCC 3 cut(s) 272, 761, 1389
SmiI ATTTAAAT 1 cut(s) 529
SmiMI CAYNNNNRTG 1 cut(s) 1032
SmlI CTYRAG 2 cut(s) 175, 881
SmoI CTYRAG 2 cut(s) 175, 881
SseBI AGGCCT 1 cut(s) 877
SsiI CCGC 2 cut(s) 454, 1105
SspMI CTAG 2 cut(s) 840, 1302
StuI AGGCCT 1 cut(s) 877
StyI CCWWGG 3 cut(s) 39, 905, 924
SwaI ATTTAAAT 1 cut(s) 529
TaaI ACNGT 6 cut(s) 150, 732, 1066, 1296, 1369, 1505
TaiI ACGT 1 cut(s) 283
TaqI TCGA 3 cut(s) 315, 577, 721
TfiI GAWTC 2 cut(s) 1193, 1223
Tru1I TTAA 6 cut(s) 528, 533, 738, 776, 915, 1524
Tru9I TTAA 6 cut(s) 528, 533, 738, 776, 915, 1524
TscAI CASTG 1 cut(s) 1510
TseFI GTSAC 1 cut(s) 399
TseI GCWGC 2 cut(s) 284, 809
Tsp45I GTSAC 1 cut(s) 399
TspDTI ATGAA 5 cut(s) 105, 122, 1431, 1467, 1473
TspGWI ACGGA 1 cut(s) 658
TspRI CASTG 1 cut(s) 1510
VpaK11BI GGWCC 3 cut(s) 272, 761, 1389
VspI ATTAAT 1 cut(s) 1524
XapI RAATTY 3 cut(s) 369, 524, 698
XceI RCATGY 2 cut(s) 263, 1031
XcmI CCANNNNNNNNNTGG 2 cut(s) 46, 1599
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 2 cut(s) 840, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.