RLG00000034985

Splicing factor U2af small subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006476
Physical Location & Seq
Reverse (-)
58352536 .. 58630825
278290 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034985

Sequence Viewer

Length: 537 bp
ATGGCGGAGCACTTGGCCTCAATCTTTGGGACGGAGAAGGACAGAGTGAACTGCCCCTTCTACTTCAAAATAGGGGCATGCAGGCACGGCGATCGGTGCTCCAGGCTCCACACGAAGCCCAGCGTCAGCCCGACTCTGTTGCTCTCCAATATGTACCAGAGGCCCGATATGATCACCCCCGGCGTCGACGCCCAGGGCCAGCCTATCGACCCCCGCCAGATGCAAACCCATTTCGAGAAATTTTATGAGGATCTCTTTCAAGAGTTGAATGAGTATGGGGAAATTGAAAGCCTGAATGTCTGCGACAACCTAGCTGACCACATGTTGGGCAATGTGAATGTTCAGTTCATGGAGGAAGAACATGCTGCAAATGCACTCAAGAATTTGACTGGAAGATTTTATGCTGGGCGCCCTATCATTGTGGACTTCTCTCCGGTGACAGACTTCCGTGAAGCCACCTGCAGTCAATATGAGGAAAATACATGCAATCGTGGTGGATACTGCAACTTTATGCATCTGAAAAGGATTGGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05131 map03040 map05131
Pfam Domains
Protein Families
RRM_1 zf-CCCH

Protein Analysis

179

Amino Acids

20.42

Weight (kDa)

5.62

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 14 - 38 1.1e-07 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 467
Acc36I ACCTGC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 408
AccB7I CCANNNNNTGG 1 cut(s) 325
AccI GTMKAC 1 cut(s) 186
AciI CCGC 2 cut(s) 5, 214
AclWI GGATC 1 cut(s) 258
AcsI RAATTY 2 cut(s) 239, 382
AcyI GRCGYC 3 cut(s) 183, 189, 409
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 325
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 4 cut(s) 67, 260, 268, 287
AjnI CCWGG 2 cut(s) 101, 192
AluBI AGCT 1 cut(s) 314
AluI AGCT 1 cut(s) 314
Alw21I GWGCWC 2 cut(s) 12, 101
AlwI GGATC 1 cut(s) 258
AoxI GGCC 3 cut(s) 15, 161, 196
ApeKI GCWGC 1 cut(s) 365
ApoI RAATTY 2 cut(s) 239, 382
AspLEI GCGC 1 cut(s) 411
AspS9I GGNCC 2 cut(s) 162, 196
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 166, 448
BanI GGYRCC 1 cut(s) 408
Bbv12I GWGCWC 2 cut(s) 12, 101
BbvI GCAGC 1 cut(s) 352
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 4 cut(s) 74, 108, 121, 155
BciT130I CCWGG 2 cut(s) 103, 194
BciVI GTATCC 1 cut(s) 491
BclI TGATCA 1 cut(s) 171
BcnI CCSGG 1 cut(s) 180
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 460
BfoI RGCGCY 1 cut(s) 412
BfuAI ACCTGC 1 cut(s) 467
BfuI GTATCC 1 cut(s) 491
BisI GCNGC 1 cut(s) 366
BlsI GCNGC 1 cut(s) 367
Bme1390I CCNGG 3 cut(s) 103, 180, 194
BmgT120I GGNCC 2 cut(s) 162, 196
BmiI GGNNCC 2 cut(s) 107, 410
BmrFI CCNGG 3 cut(s) 103, 180, 194
BmsI GCATC 2 cut(s) 210, 523
BpmI CTGGAG 1 cut(s) 85
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 180
BsaHI GRCGYC 3 cut(s) 183, 189, 409
BsaJI CCNNGG 3 cut(s) 178, 192, 193
BsaWI WCCGGW 1 cut(s) 433
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 337
BseBI CCWGG 2 cut(s) 103, 194
BseDI CCNNGG 3 cut(s) 178, 192, 193
BseLI CCNNNNNNNGG 1 cut(s) 325
BseMI GCAATG 1 cut(s) 337
BseNI ACTGG 1 cut(s) 394
BseXI GCAGC 1 cut(s) 352
BseYI CCCAGC 2 cut(s) 119, 404
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 3 cut(s) 17, 163, 198
BshNI GGYRCC 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 12, 101
BsiSI CCGG 2 cut(s) 180, 434
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 1 cut(s) 325
BsmFI GGGAC 1 cut(s) 43
BsnI GGCC 3 cut(s) 17, 163, 198
Bsp1286I GDGCHC 2 cut(s) 12, 101
Bsp143I GATC 3 cut(s) 91, 171, 250
BspACI CCGC 2 cut(s) 5, 214
BspANI GGCC 3 cut(s) 17, 163, 198
BspLI GGNNCC 2 cut(s) 107, 410
BspMAI CTGCAG 1 cut(s) 464
BspMI ACCTGC 1 cut(s) 467
BspPI GGATC 1 cut(s) 258
BspT107I GGYRCC 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 337
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 3 cut(s) 178, 192, 193
BssMI GATC 3 cut(s) 91, 171, 250
BssNI GRCGYC 3 cut(s) 183, 189, 409
Bst2UI CCWGG 2 cut(s) 103, 194
BstACI GRCGYC 3 cut(s) 183, 189, 409
BstC8I GCNNGC 3 cut(s) 79, 83, 200
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 1 cut(s) 411
BstKTI GATC 3 cut(s) 94, 174, 253
BstMBI GATC 3 cut(s) 91, 171, 250
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 3 cut(s) 87, 96, 371
BstNI CCWGG 2 cut(s) 103, 194
BstNSI RCATGY 4 cut(s) 81, 325, 365, 486
BstSCI CCNGG 3 cut(s) 101, 178, 192
BstSFI CTRYAG 1 cut(s) 460
BstV1I GCAGC 1 cut(s) 352
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BsuI GTATCC 1 cut(s) 491
BsuRI GGCC 3 cut(s) 17, 163, 198
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 3 cut(s) 79, 83, 200
CfoI GCGC 1 cut(s) 411
Cfr13I GGNCC 2 cut(s) 162, 196
CseI GACGC 3 cut(s) 112, 172, 197
Csp6I GTAC 1 cut(s) 154
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviAII CATG 5 cut(s) 78, 322, 349, 362, 483
CviQI GTAC 1 cut(s) 154
DinI GGCGCC 1 cut(s) 410
DpnI GATC 3 cut(s) 93, 173, 252
DpnII GATC 3 cut(s) 91, 171, 250
EciI GGCGGA 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 101, 192
EcoT22I ATGCAT 1 cut(s) 516
EgeI GGCGCC 1 cut(s) 410
EheI GGCGCC 1 cut(s) 410
FaeI CATG 5 cut(s) 81, 325, 352, 365, 486
FaqI GGGAC 1 cut(s) 43
FatI CATG 5 cut(s) 77, 321, 348, 361, 482
FauI CCCGC 1 cut(s) 221
FbaI TGATCA 1 cut(s) 171
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 1 cut(s) 366
Fsp4HI GCNGC 1 cut(s) 366
FspBI CTAG 1 cut(s) 311
GlaI GCGC 1 cut(s) 410
GluI GCNGC 1 cut(s) 366
GsaI CCCAGC 2 cut(s) 123, 408
GsuI CTGGAG 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 3 cut(s) 17, 163, 198
HapII CCGG 2 cut(s) 180, 434
HgaI GACGC 3 cut(s) 112, 172, 197
HhaI GCGC 1 cut(s) 411
Hin1I GRCGYC 3 cut(s) 183, 189, 409
Hin1II CATG 5 cut(s) 81, 325, 352, 365, 486
Hin6I GCGC 1 cut(s) 409
HinP1I GCGC 1 cut(s) 409
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 1 cut(s) 133
HpaII CCGG 2 cut(s) 180, 434
HphI GGTGA 2 cut(s) 166, 448
Hpy166II GTNNAC 3 cut(s) 49, 187, 424
Hpy188I TCNGA 1 cut(s) 519
Hpy188III TCNNGA 3 cut(s) 235, 260, 379
Hpy8I GTNNAC 3 cut(s) 49, 187, 424
Hpy99I CGWCG 2 cut(s) 188, 191
HpyAV CCTTC 2 cut(s) 31, 67
HpyCH4V TGCA 8 cut(s) 81, 223, 368, 374, 462, 486, 504, 514
HpyF10VI GCNNNNNNNGC 3 cut(s) 87, 96, 371
Hsp92I GRCGYC 3 cut(s) 183, 189, 409
Hsp92II CATG 5 cut(s) 81, 325, 352, 365, 486
HspAI GCGC 1 cut(s) 409
KasI GGCGCC 1 cut(s) 408
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 3 cut(s) 91, 171, 250
LmnI GCTCC 3 cut(s) 7, 104, 111
Lsp1109I GCAGC 1 cut(s) 352
LweI GCATC 2 cut(s) 210, 523
MaeI CTAG 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 436
MalI GATC 3 cut(s) 93, 173, 252
MboI GATC 3 cut(s) 91, 171, 250
MboII GAAGA 2 cut(s) 368, 405
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 2 cut(s) 12, 101
MluCI AATT 3 cut(s) 239, 282, 382
Mly113I GGCGCC 1 cut(s) 409
MlyI GAGTC 1 cut(s) 127
MnlI CCTC 6 cut(s) 28, 153, 241, 346, 466, 525
Mph1103I ATGCAT 1 cut(s) 516
MspI CCGG 2 cut(s) 180, 434
MspR9I CCNGG 3 cut(s) 103, 180, 194
MvaI CCWGG 2 cut(s) 103, 194
MwoI GCNNNNNNNGC 3 cut(s) 87, 96, 371
NarI GGCGCC 1 cut(s) 409
NciI CCSGG 1 cut(s) 180
NdeII GATC 3 cut(s) 91, 171, 250
NlaIII CATG 5 cut(s) 81, 325, 352, 365, 486
NlaIV GGNNCC 2 cut(s) 107, 410
NmuCI GTSAC 1 cut(s) 436
NsiI ATGCAT 1 cut(s) 516
NspI RCATGY 4 cut(s) 81, 325, 365, 486
PaeI GCATGC 1 cut(s) 81
PaqCI CACCTGC 1 cut(s) 467
PasI CCCWGGG 1 cut(s) 193
PciI ACATGT 1 cut(s) 321
PflMI CCANNNNNTGG 1 cut(s) 325
PkrI GCNGC 1 cut(s) 367
Ple19I CGATCG 1 cut(s) 94
PleI GAGTC 1 cut(s) 127
PluTI GGCGCC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 127
PscI ACATGT 1 cut(s) 321
Psp6I CCWGG 2 cut(s) 101, 192
PspFI CCCAGC 2 cut(s) 119, 404
PspGI CCWGG 2 cut(s) 101, 192
PspN4I GGNNCC 2 cut(s) 107, 410
PspPI GGNCC 2 cut(s) 162, 196
PstI CTGCAG 1 cut(s) 464
PsuI RGATCY 1 cut(s) 250
PvuI CGATCG 1 cut(s) 94
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SalI GTCGAC 1 cut(s) 185
SatI GCNGC 1 cut(s) 366
Sau3AI GATC 3 cut(s) 91, 171, 250
Sau96I GGNCC 2 cut(s) 162, 196
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 3 cut(s) 103, 180, 194
SduI GDGCHC 2 cut(s) 12, 101
SetI ASST 4 cut(s) 312, 316, 461, 536
SfaNI GCATC 2 cut(s) 210, 523
SfcI CTRYAG 1 cut(s) 460
SfoI GGCGCC 1 cut(s) 410
SgrDI CGTCGACG 1 cut(s) 185
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SphI GCATGC 1 cut(s) 81
Sse9I AATT 3 cut(s) 239, 282, 382
SsiI CCGC 2 cut(s) 5, 214
SspDI GGCGCC 1 cut(s) 408
SspMI CTAG 1 cut(s) 311
StyD4I CCNGG 3 cut(s) 101, 178, 192
TaqI TCGA 3 cut(s) 186, 207, 234
TasI AATT 3 cut(s) 239, 282, 382
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 1 cut(s) 337
TspGWI ACGGA 2 cut(s) 47, 437
Van91I CCANNNNNTGG 1 cut(s) 325
XapI RAATTY 2 cut(s) 239, 382
XceI RCATGY 4 cut(s) 81, 325, 365, 486
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 311
Zsp2I ATGCAT 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.