Rh5AG362400

Splicing factor U2af small subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006476
Physical Location & Seq
Reverse (-)
61141263 .. 61145138
3876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG362400.1

Sequence Viewer

Length: 714 bp
ATGTATATTTGTGCAAAATTGCAGGAATTTTATGAGGATCTCTTTCAAGAGCTGAGTAAGTTTGGAGAAATTGAAAGCCTGAATGTCTGCGACAACCTAGCTGACCACATGGTGGGCAATGTGTATGTTCAGTTCAGGGAGGAAGAACATGCTGCAAATGCACTCAAGAATCTGACTGGAAGATTTTATGCTGGGCGTCCTATCATTGTGGACTTCTCTCCGGTGACAGACTTCCGTGAAGCCACCTGCAGGCAATATGAGGAAAATACATGCAATCGTGGTGGATACTGCAACTTTATGCATCTGAAAAGGATTGGGAGGGAATTGAGGCGTGAGTTATTTAGGAGCTACCGGCGAAGGCATAGCCGGAGTCGAAGCAGGAGCCGCAGCCCTTACAGGCATCGTAGCTATGAAGAACGCCCTCATGGTAGCCATGGTCATAGCAGAAGGTATGATGATGGGGATCGGTATCCTGAGAGAAGGAACAGGACTGTAAGCCCTGGCCGAAGTAAGCGGGAACGCAGTAGAAGCAGGAGCACTGAAAGGAGGAGGAGAAGGAACCCCAGTCCAGTTAGAGAAGGCAGTGAGGAGAGACGAGCCAAAATTGGACAGTGGAACAGGGAAAGAGAACAAAATGAAATTGCTTCCAAGGTCACCTCTGTCAGAAGTGACAATGGGCATGCTCAAACTGAAAATCAGAACGGCTATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05131 map03040 map05131
Pfam Domains
Protein Families
RRM_1 zf-CCCH

Protein Analysis

237

Amino Acids

28.36

Weight (kDa)

9.96

Isoelectric Point (pI)

92.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 12 - 68 4.3e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 254
Acc36I ACCTGC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 112
AciI CCGC 2 cut(s) 385, 514
AclWI GGATC 2 cut(s) 45, 471
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 1 cut(s) 26
AcyI GRCGYC 1 cut(s) 196
AdeI CACNNNGTG 1 cut(s) 112
AfiI CCNNNNNNNGG 2 cut(s) 112, 249
AgsI TTSAA 2 cut(s) 47, 74
AjnI CCWGG 1 cut(s) 499
AluBI AGCT 4 cut(s) 52, 101, 348, 408
AluI AGCT 4 cut(s) 52, 101, 348, 408
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 1 cut(s) 586
AlwI GGATC 2 cut(s) 45, 471
AoxI GGCC 1 cut(s) 502
ApeKI GCWGC 2 cut(s) 152, 387
ApoI RAATTY 1 cut(s) 26
AsuHPI GGTGA 2 cut(s) 235, 646
Bbv12I GWGCWC 1 cut(s) 539
BbvI GCAGC 2 cut(s) 139, 399
BccI CCATC 1 cut(s) 452
BciT130I CCWGG 1 cut(s) 501
BciVI GTATCC 2 cut(s) 278, 480
BcoDI GTCTC 1 cut(s) 586
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 247
BfuAI ACCTGC 1 cut(s) 254
BfuI GTATCC 2 cut(s) 278, 480
BisI GCNGC 3 cut(s) 153, 385, 388
BlsI GCNGC 3 cut(s) 154, 386, 389
Bme1390I CCNGG 1 cut(s) 501
BmiI GGNNCC 2 cut(s) 383, 560
BmrFI CCNGG 1 cut(s) 501
BmrI ACTGGG 1 cut(s) 558
BmsI GCATC 2 cut(s) 310, 409
BmuI ACTGGG 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 149
BsaBI GATNNNNATC 2 cut(s) 462, 468
BsaHI GRCGYC 1 cut(s) 196
BsaJI CCNNGG 3 cut(s) 433, 499, 648
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 249
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 3 cut(s) 181, 564, 569
Bse3DI GCAATG 1 cut(s) 124
Bse8I GATNNNNATC 2 cut(s) 462, 468
BseBI CCWGG 1 cut(s) 501
BseDI CCNNGG 3 cut(s) 433, 499, 648
BseJI GATNNNNATC 2 cut(s) 462, 468
BseLI CCNNNNNNNGG 2 cut(s) 112, 249
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 2 cut(s) 44, 465
BseNI ACTGG 3 cut(s) 181, 564, 569
BseRI GAGGAG 3 cut(s) 562, 565, 602
BseXI GCAGC 2 cut(s) 139, 399
BseYI CCCAGC 1 cut(s) 191
BshFI GGCC 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 539
BsiSI CCGG 3 cut(s) 221, 352, 367
BslI CCNNNNNNNGG 2 cut(s) 112, 249
BsmAI GTCTC 1 cut(s) 586
BsmBI CGTCTC 1 cut(s) 586
BsnI GGCC 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 2 cut(s) 37, 463
Bsp19I CCATGG 1 cut(s) 433
BspACI CCGC 2 cut(s) 385, 514
BspANI GGCC 1 cut(s) 504
BspCNI CTCAG 2 cut(s) 45, 466
BspLI GGNNCC 2 cut(s) 383, 560
BspMAI CTGCAG 1 cut(s) 251
BspMI ACCTGC 1 cut(s) 254
BspPI GGATC 2 cut(s) 45, 471
BsrDI GCAATG 1 cut(s) 124
BsrFI RCCGGY 1 cut(s) 351
BsrI ACTGG 3 cut(s) 181, 564, 569
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 3 cut(s) 433, 499, 648
BssMI GATC 2 cut(s) 37, 463
BssNI GRCGYC 1 cut(s) 196
BssT1I CCWWGG 2 cut(s) 433, 648
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 2 cut(s) 493, 612
BstACI GRCGYC 1 cut(s) 196
BstC8I GCNNGC 2 cut(s) 251, 681
BstDEI CTNAG 2 cut(s) 53, 474
BstDSI CCRYGG 1 cut(s) 433
BstEII GGTNACC 1 cut(s) 652
BstKTI GATC 2 cut(s) 40, 466
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 2 cut(s) 37, 463
BstMWI GCNNNNNNNGC 3 cut(s) 158, 384, 528
BstNI CCWGG 1 cut(s) 501
BstNSI RCATGY 3 cut(s) 152, 273, 683
BstPI GGTNACC 1 cut(s) 652
BstSCI CCNGG 1 cut(s) 499
BstSFI CTRYAG 1 cut(s) 247
BstV1I GCAGC 2 cut(s) 139, 399
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BsuI GTATCC 2 cut(s) 278, 480
BsuRI GGCC 1 cut(s) 504
BtgI CCRYGG 1 cut(s) 433
BtsI GCAGTG 1 cut(s) 589
BtsIMutI CAGTG 3 cut(s) 537, 589, 617
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 2 cut(s) 251, 681
Cfr10I RCCGGY 1 cut(s) 351
CseI GACGC 1 cut(s) 185
CspCI CAANNNNNGTGG 2 cut(s) 262, 297
CviAII CATG 6 cut(s) 109, 149, 270, 425, 434, 680
DdeI CTNAG 2 cut(s) 53, 474
DpnI GATC 2 cut(s) 39, 465
DpnII GATC 2 cut(s) 37, 463
DraIII CACNNNGTG 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 502
Eco130I CCWWGG 2 cut(s) 433, 648
Eco91I GGTNACC 1 cut(s) 652
EcoO65I GGTNACC 1 cut(s) 652
EcoRII CCWGG 1 cut(s) 499
EcoT14I CCWWGG 2 cut(s) 433, 648
EcoT22I ATGCAT 1 cut(s) 303
ErhI CCWWGG 2 cut(s) 433, 648
Esp3I CGTCTC 1 cut(s) 586
FaeI CATG 6 cut(s) 112, 152, 273, 428, 437, 683
FatI CATG 6 cut(s) 108, 148, 269, 424, 433, 679
FauI CCCGC 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 153, 385, 388
Fsp4HI GCNGC 3 cut(s) 153, 385, 388
FspBI CTAG 1 cut(s) 98
GluI GCNGC 3 cut(s) 153, 385, 388
GsaI CCCAGC 1 cut(s) 195
HaeIII GGCC 1 cut(s) 504
HapII CCGG 3 cut(s) 221, 352, 367
HgaI GACGC 1 cut(s) 185
Hin1I GRCGYC 1 cut(s) 196
Hin1II CATG 6 cut(s) 112, 152, 273, 428, 437, 683
HinfI GANTC 2 cut(s) 169, 370
HpaII CCGG 3 cut(s) 221, 352, 367
HphI GGTGA 2 cut(s) 235, 646
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 4 cut(s) 174, 306, 665, 699
Hpy188III TCNNGA 3 cut(s) 47, 166, 473
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 5 cut(s) 351, 441, 474, 549, 572
HpyCH4III ACNGT 2 cut(s) 493, 612
HpyCH4V TGCA 8 cut(s) 14, 22, 155, 161, 249, 273, 291, 301
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 384, 528
HpyF3I CTNAG 2 cut(s) 53, 474
Hsp92I GRCGYC 1 cut(s) 196
Hsp92II CATG 6 cut(s) 112, 152, 273, 428, 437, 683
Kzo9I GATC 2 cut(s) 37, 463
LmnI GCTCC 3 cut(s) 345, 381, 534
Lsp1109I GCAGC 2 cut(s) 139, 399
LweI GCATC 2 cut(s) 310, 409
MaeI CTAG 1 cut(s) 98
MaeIII GTNAC 3 cut(s) 223, 652, 668
MalI GATC 2 cut(s) 39, 465
MboI GATC 2 cut(s) 37, 463
MboII GAAGA 3 cut(s) 155, 192, 425
MflI RGATCY 1 cut(s) 37
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 6 cut(s) 17, 26, 69, 323, 603, 639
MlyI GAGTC 1 cut(s) 379
Mph1103I ATGCAT 1 cut(s) 303
MspI CCGG 3 cut(s) 221, 352, 367
MspR9I CCNGG 1 cut(s) 501
MvaI CCWGG 1 cut(s) 501
MwoI GCNNNNNNNGC 3 cut(s) 158, 384, 528
NcoI CCATGG 1 cut(s) 433
NdeII GATC 2 cut(s) 37, 463
NlaIII CATG 6 cut(s) 112, 152, 273, 428, 437, 683
NlaIV GGNNCC 2 cut(s) 383, 560
NmuCI GTSAC 3 cut(s) 223, 652, 668
NsiI ATGCAT 1 cut(s) 303
NspI RCATGY 3 cut(s) 152, 273, 683
PaeI GCATGC 1 cut(s) 683
PaqCI CACCTGC 1 cut(s) 254
PfeI GAWTC 1 cut(s) 169
PflMI CCANNNNNTGG 1 cut(s) 112
PkrI GCNGC 3 cut(s) 154, 386, 389
PleI GAGTC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 378
Psp6I CCWGG 1 cut(s) 499
PspEI GGTNACC 1 cut(s) 652
PspFI CCCAGC 1 cut(s) 191
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 2 cut(s) 383, 560
PsrI GAACNNNNNNTAC 2 cut(s) 116, 148
PstI CTGCAG 1 cut(s) 251
PsuI RGATCY 1 cut(s) 37
SatI GCNGC 3 cut(s) 153, 385, 388
Sau3AI GATC 2 cut(s) 37, 463
SbfI CCTGCAGG 1 cut(s) 251
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 501
SdaI CCTGCAGG 1 cut(s) 251
SduI GDGCHC 1 cut(s) 539
SetI ASST 9 cut(s) 54, 99, 103, 248, 350, 410, 452, 654, 659
SfaNI GCATC 2 cut(s) 310, 409
SfcI CTRYAG 1 cut(s) 247
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
SphI GCATGC 1 cut(s) 683
Sse8387I CCTGCAGG 1 cut(s) 251
Sse9I AATT 6 cut(s) 17, 26, 69, 323, 603, 639
SsiI CCGC 2 cut(s) 385, 514
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 499
StyI CCWWGG 2 cut(s) 433, 648
TaaI ACNGT 2 cut(s) 493, 612
TaqI TCGA 1 cut(s) 373
TasI AATT 6 cut(s) 17, 26, 69, 323, 603, 639
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 1 cut(s) 169
TscAI CASTG 3 cut(s) 544, 589, 617
TseFI GTSAC 3 cut(s) 223, 652, 668
TseI GCWGC 2 cut(s) 152, 387
Tsp45I GTSAC 3 cut(s) 223, 652, 668
TspDTI ATGAA 2 cut(s) 426, 651
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 3 cut(s) 544, 589, 617
Van91I CCANNNNNTGG 1 cut(s) 112
XapI RAATTY 1 cut(s) 26
XceI RCATGY 3 cut(s) 152, 273, 683
XspI CTAG 1 cut(s) 98
Zsp2I ATGCAT 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.