Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0008925

Physical Location & Seq

Genomic Coordinates Forward (+)

67823342 .. 67826946

Gene Structure 3605 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000035506

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGAGTTGCTTTTGCTGTAAAAAAAATTCAAAAAAAGAAGCAGAGAGGAACAATAAGAAGAACAACAACACCCACATCAACTGTAGCAAATCAAATCGTCTAGATACTTCAAAAGTGAATTCAGAGGGAAATGTAAAAAAGGAATTGGGTGTAAAAAAGGAGGAGGTCTCTAAGGTTGACCAGTTATCGATCGATGTAAAGGAGTTGAACATAAAGGAGGAGGTTTCTAAGGATGGAAAGACTAATGGCAGGCGGCCACAGGCATTTACGTTGGAGGAAATGGAAGTAGCAACCCAAAACTTCAGTCCTGACTGCTTTATAGGTGAAGGAGGATTCGGCAAAGTTTACAAGGGTCACTTAGAGAAAACTAACCAGGTTGTTGCTATCAAGCAGCTTGATCGCAATGGTTGCCAAGGGATCAGGGAATTTGTTGTTGAAGTATTGACACTAGGTTTGGCAGATCATCAGAATCTTGTCAAATTGATTGGCTTTTGTGCTGAGGGGGATCAAAGGCTATTGGTTTATGAATATATGCCATTTGGATCTTTGGAAAACCATTTGCATGATCTTCAAGCTGGCGGGAATGTTCTGGATTGGAATACAAGAATGAAGATCGCAGCTGGTGCAGCAAGAGGTTTAGAGTATCTGCATGAGAAAATGCAGCCTGCTATCATATATCGCGACCTGAAATGCTCAAACATTTTGCTTGGTGAGGATTATCATCCAAAGCTATCTGATTTTGGCTTGGCCAAAGTAGGTCCGAGTGGAGATAAGACTCATGTTTCAACTAGGGTTATGGGAACATATGGGTACTGTGCACCAGATTATGCAATGACAGGTCAGCTGACTTTTAAATCAGATGTTTATAGCTTCGGGGTGGTTCTTTTGGAGCTTATTACAGGCCGAAAAGCTATTGATGGCACAAGACCTCTCAGGGAGCAAAACCTCGTAGCATGGGCAAGACCCTTGTTCAGAGACCGGAAGAAATTCTCACAGATGGTGGATCCGTTGCTCCACGGTCAGTATCCTGTCAGGGGATTATACCAAGCTCTTGCTATTGCTGCGATGTGCGTTCAGGAGCAGCCTAATATGCGGCCTGTCATAACCGATATAGTGACAGCACTAAATTACCTTGCTTCCCAGAAATATGACCCCCAATTCCATCCAGTACAAAGCTCCAGAAGGAGTCCATCTTCTCCTAAAGTGCTGCTGCCATTCTCAATATTCACATACAGAGAATTTCTACTCTTCTCTCTTCTCCCCCGCCGTGTACATCAAAGTAACTGCAGAACCACAGCTCTTCCTTTGCGCAAGCTTTTTCCGAAAAACAAATTGGAAATGTATGTACCTAGTCTCGCCATGCAGGTAATGTGGAATATCATTGCTGGATGGAATGTGAAAACATGCATCATGGTGAGTTTTGAGGCAAGGAGGGAACCTGGAGGTTCTACTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

486

Amino Acids

54.71

Weight (kDa)

9.16

Isoelectric Point (pI)

37.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	106 - 372	2.8e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	107 - 374	3.1e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008925)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07870 AT1G07870 AT2G28590 AT2G28590
fragaria_vesca	FvH4_3g34150 FvH4_3g34150 FvH4_3g34150 FvH4_3g34150
malus_domestica	MD03G1107300.v1.1 MD11G1120000.v1.1
prunus_persica	Prupe.6G090100_v2.0.a1 Prupe.6G090100_v2.0.a1
pyrus_communis	pycom03g08680 pycom11g10140
rosa_chinensis	RchiOBHm_Chr5g0061791
rosa_laevigata	RLG00000035506
rosa_multiflora	Rmu_ssc0000275.1_g000001
rosa_roxburghii	Rroxscaffold_1G00018940
rosa_rugosa	Rorug05G0343900 Rorug05G0344000
rosa_samantha	Rh5AG404800
rosa_wichuraiana	Rw5G038020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1310
Acc36I	ACCTGC	1 cut(s)	1354
AccII	CGCG	1 cut(s)	681
AciI	CCGC	4 cut(s)	253, 579, 1093, 1264
AclWI	GGATC	5 cut(s)	425, 513, 550, 998, 1011
AcoI	YGGCCR	2 cut(s)	254, 747
AcsI	RAATTY	5 cut(s)	25, 118, 425, 986, 1238
AcuI	CTGAAG	1 cut(s)	286
AfaI	GTAC	4 cut(s)	812, 1170, 1272, 1347
AfiI	CCNNNNNNNGG	1 cut(s)	1034
AgsI	TTSAA	6 cut(s)	30, 111, 208, 437, 572, 786
AjnI	CCWGG	2 cut(s)	372, 1438
AjuI	GAANNNNNNNTTGG	2 cut(s)	1316, 1348
Alw21I	GWGCWC	1 cut(s)	820
Alw26I	GTCTC	3 cut(s)	172, 969, 1358
Alw44I	GTGCAC	1 cut(s)	816
AlwI	GGATC	5 cut(s)	425, 513, 550, 998, 1011
AoxI	GGCC	4 cut(s)	254, 747, 901, 1094
ApaLI	GTGCAC	1 cut(s)	816
ApeKI	GCWGC	8 cut(s)	391, 617, 626, 661, 1061, 1081, 1207, 1210
ApoI	RAATTY	5 cut(s)	25, 118, 425, 986, 1238
ArsI	GACNNNNNNTTYG	4 cut(s)	289, 321, 436, 468
Asp700I	GAANNNNTTC	1 cut(s)	986
AspLEI	GCGC	1 cut(s)	1311
AspS9I	GGNCC	1 cut(s)	758
AsuHPI	GGTGA	3 cut(s)	335, 722, 1426
AvaII	GGWCC	1 cut(s)	758
BaeGI	GKGCMC	1 cut(s)	820
BalI	TGGCCA	1 cut(s)	749
BamHI	GGATCC	1 cut(s)	1003
Bbv12I	GWGCWC	1 cut(s)	820
BbvCI	CCTCAGC	1 cut(s)	498
BbvI	GCAGC	8 cut(s)	403, 629, 638, 673, 1048, 1093, 1194, 1197
BccI	CCATC	6 cut(s)	227, 911, 991, 1170, 1198, 1383
BceAI	ACGGC	1 cut(s)	1251
BcgI	CGANNNNNNTGC	2 cut(s)	380, 414
BciT130I	CCWGG	2 cut(s)	374, 1440
BciVI	GTATCC	1 cut(s)	1035
BcoDI	GTCTC	3 cut(s)	172, 969, 1358
BfaI	CTAG	4 cut(s)	101, 449, 789, 1350
BfmI	CTRYAG	2 cut(s)	82, 1285
BfuAI	ACCTGC	1 cut(s)	1354
BfuI	GTATCC	1 cut(s)	1035
Bme1390I	CCNGG	2 cut(s)	374, 1440
Bme18I	GGWCC	1 cut(s)	758
BmgT120I	GGNCC	1 cut(s)	758
BmiI	GGNNCC	2 cut(s)	1005, 1437
BmrFI	CCNGG	2 cut(s)	374, 1440
BmsI	GCATC	1 cut(s)	1416
BplI	GAGNNNNNCTC	2 cut(s)	152, 184
BpmI	CTGGAG	1 cut(s)	1162
Bpu10I	CCTNAGC	1 cut(s)	498
Bsa29I	ATCGAT	2 cut(s)	188, 192
BsaBI	GATNNNNATC	1 cut(s)	720
BsaI	GGTCTC	2 cut(s)	172, 969
BsaJI	CCNNGG	2 cut(s)	412, 1015
BsaWI	WCCGGW	1 cut(s)	978
Bsc4I	CCNNNNNNNGG	1 cut(s)	1034
Bse1I	ACTGG	2 cut(s)	181, 1166
Bse3DI	GCAATG	3 cut(s)	409, 837, 1380
Bse8I	GATNNNNATC	1 cut(s)	720
BseBI	CCWGG	2 cut(s)	374, 1440
BseCI	ATCGAT	2 cut(s)	188, 192
BseDI	CCNNGG	2 cut(s)	412, 1015
BseGI	GGATG	4 cut(s)	238, 721, 1162, 1394
BseJI	GATNNNNATC	1 cut(s)	720
BseLI	CCNNNNNNNGG	1 cut(s)	1034
BseMI	GCAATG	3 cut(s)	409, 837, 1380
BseMII	CTCAG	2 cut(s)	489, 946
BseNI	ACTGG	2 cut(s)	181, 1166
BseRI	GAGGAG	2 cut(s)	176, 233
BseSI	GKGCMC	1 cut(s)	820
BseXI	GCAGC	8 cut(s)	403, 629, 638, 673, 1048, 1093, 1194, 1197
BsgI	GTGCAG	1 cut(s)	645
Bsh1236I	CGCG	1 cut(s)	681
Bsh1285I	CGRYCG	1 cut(s)	192
BshFI	GGCC	4 cut(s)	256, 749, 903, 1096
BshVI	ATCGAT	2 cut(s)	188, 192
BsiEI	CGRYCG	1 cut(s)	192
BsiHKAI	GWGCWC	1 cut(s)	820
BsiSI	CCGG	1 cut(s)	979
BslI	CCNNNNNNNGG	1 cut(s)	1034
BsmAI	GTCTC	3 cut(s)	172, 969, 1358
BsnI	GGCC	4 cut(s)	256, 749, 903, 1096
Bso31I	GGTCTC	2 cut(s)	172, 969
Bsp1286I	GDGCHC	1 cut(s)	820
Bsp1407I	TGTACA	1 cut(s)	1270
Bsp143I	GATC	9 cut(s)	189, 397, 417, 460, 505, 542, 565, 612, 1003
Bsp68I	TCGCGA	1 cut(s)	681
BspACI	CCGC	4 cut(s)	253, 579, 1093, 1264
BspANI	GGCC	4 cut(s)	256, 749, 903, 1096
BspCNI	CTCAG	2 cut(s)	490, 945
BspDI	ATCGAT	2 cut(s)	188, 192
BspFNI	CGCG	1 cut(s)	681
BspLI	GGNNCC	2 cut(s)	1005, 1437
BspMAI	CTGCAG	1 cut(s)	1289
BspMI	ACCTGC	1 cut(s)	1354
BspPI	GGATC	5 cut(s)	425, 513, 550, 998, 1011
BspQI	GCTCTTC	1 cut(s)	1305
BspTNI	GGTCTC	2 cut(s)	172, 969
BsrDI	GCAATG	3 cut(s)	409, 837, 1380
BsrGI	TGTACA	1 cut(s)	1270
BsrI	ACTGG	2 cut(s)	181, 1166
BssECI	CCNNGG	2 cut(s)	412, 1015
BssMI	GATC	9 cut(s)	189, 397, 417, 460, 505, 542, 565, 612, 1003
BssT1I	CCWWGG	1 cut(s)	412
Bst2UI	CCWGG	2 cut(s)	374, 1440
Bst4CI	ACNGT	3 cut(s)	83, 815, 1019
Bst6I	CTCTTC	3 cut(s)	1253, 1260, 1305
BstAPI	GCANNNNNTGC	2 cut(s)	408, 623
BstAUI	TGTACA	1 cut(s)	1270
BstC8I	GCNNGC	4 cut(s)	251, 577, 666, 1313
BstDEI	CTNAG	5 cut(s)	171, 228, 358, 498, 932
BstDSI	CCRYGG	1 cut(s)	1015
BstF5I	GGATG	4 cut(s)	238, 721, 1162, 1394
BstFNI	CGCG	1 cut(s)	681
BstHHI	GCGC	1 cut(s)	1311
BstKTI	GATC	9 cut(s)	192, 400, 420, 463, 508, 545, 568, 615, 1006
BstMAI	GTCTC	3 cut(s)	172, 969, 1358
BstMBI	GATC	9 cut(s)	189, 397, 417, 460, 505, 542, 565, 612, 1003
BstMCI	CGRYCG	1 cut(s)	192
BstMWI	GCNNNNNNNGC	5 cut(s)	408, 623, 626, 1061, 1090
BstNI	CCWGG	2 cut(s)	374, 1440
BstNSI	RCATGY	1 cut(s)	1407
BstSCI	CCNGG	2 cut(s)	372, 1438
BstSFI	CTRYAG	2 cut(s)	82, 1285
BstSLI	GKGCMC	1 cut(s)	820
BstUI	CGCG	1 cut(s)	681
BstV1I	GCAGC	8 cut(s)	403, 629, 638, 673, 1048, 1093, 1194, 1197
BstX2I	RGATCY	2 cut(s)	542, 1003
BstYI	RGATCY	2 cut(s)	542, 1003
Bsu15I	ATCGAT	2 cut(s)	188, 192
BsuI	GTATCC	1 cut(s)	1035
BsuRI	GGCC	4 cut(s)	256, 749, 903, 1096
BsuTUI	ATCGAT	2 cut(s)	188, 192
BtgI	CCRYGG	1 cut(s)	1015
BtgZI	GCGATG	1 cut(s)	1079
BtsCI	GGATG	4 cut(s)	238, 721, 1162, 1394
BtuMI	TCGCGA	1 cut(s)	681
BveI	ACCTGC	1 cut(s)	1354
Cac8I	GCNNGC	4 cut(s)	251, 577, 666, 1313
CfoI	GCGC	1 cut(s)	1311
Cfr13I	GGNCC	1 cut(s)	758
ClaI	ATCGAT	2 cut(s)	188, 192
CsiI	ACCWGGT	1 cut(s)	372
Csp6I	GTAC	4 cut(s)	811, 1169, 1271, 1346
CviAII	CATG	7 cut(s)	563, 650, 779, 954, 1360, 1404, 1411
CviQI	GTAC	4 cut(s)	811, 1169, 1271, 1346
DdeI	CTNAG	5 cut(s)	171, 228, 358, 498, 932
DpnI	GATC	9 cut(s)	191, 399, 419, 462, 507, 544, 567, 614, 1005
DpnII	GATC	9 cut(s)	189, 397, 417, 460, 505, 542, 565, 612, 1003
DraI	TTTAAA	1 cut(s)	853
EaeI	YGGCCR	2 cut(s)	254, 747
Eam1104I	CTCTTC	3 cut(s)	1253, 1260, 1305
EarI	CTCTTC	3 cut(s)	1253, 1260, 1305
Eco130I	CCWWGG	1 cut(s)	412
Eco31I	GGTCTC	2 cut(s)	172, 969
Eco47I	GGWCC	1 cut(s)	758
Eco57I	CTGAAG	1 cut(s)	286
EcoRI	GAATTC	1 cut(s)	118
EcoRII	CCWGG	2 cut(s)	372, 1438
EcoT14I	CCWWGG	1 cut(s)	412
EcoT22I	ATGCAT	1 cut(s)	1409
ErhI	CCWWGG	1 cut(s)	412
FaeI	CATG	7 cut(s)	566, 653, 782, 957, 1363, 1407, 1414
FalI	AAGNNNNNCTT	2 cut(s)	341, 373
FatI	CATG	7 cut(s)	562, 649, 778, 953, 1359, 1403, 1410
FauI	CCCGC	2 cut(s)	572, 1271
FauNDI	CATATG	1 cut(s)	805
FokI	GGATG	4 cut(s)	245, 708, 1149, 1401
FspBI	CTAG	4 cut(s)	101, 449, 789, 1350
FspI	TGCGCA	1 cut(s)	1310
GlaI	GCGC	1 cut(s)	1310
GsuI	CTGGAG	1 cut(s)	1162
HaeIII	GGCC	4 cut(s)	256, 749, 903, 1096
HapII	CCGG	1 cut(s)	979
HhaI	GCGC	1 cut(s)	1311
Hin1II	CATG	7 cut(s)	566, 653, 782, 957, 1363, 1407, 1414
Hin6I	GCGC	1 cut(s)	1309
HinP1I	GCGC	1 cut(s)	1309
HincII	GTYRAC	1 cut(s)	178
HindII	GTYRAC	1 cut(s)	178
HindIII	AAGCTT	1 cut(s)	1313
HinfI	GANTC	4 cut(s)	333, 469, 775, 1186
HpaII	CCGG	1 cut(s)	979
HphI	GGTGA	3 cut(s)	335, 722, 1426
Hpy166II	GTNNAC	4 cut(s)	178, 346, 818, 1271
Hpy188I	TCNGA	7 cut(s)	124, 468, 736, 762, 859, 974, 1323
Hpy188III	TCNNGA	6 cut(s)	101, 308, 590, 680, 1076, 1179
Hpy8I	GTNNAC	4 cut(s)	178, 346, 818, 1271
HpyAV	CCTTC	2 cut(s)	320, 1176
HpyCH4III	ACNGT	3 cut(s)	83, 815, 1019
HpyCH4IV	ACGT	1 cut(s)	269
HpyCH4V	TGCA	9 cut(s)	562, 626, 649, 661, 818, 830, 1287, 1363, 1407
HpyF10VI	GCNNNNNNNGC	5 cut(s)	408, 623, 626, 1061, 1090
HpyF3I	CTNAG	5 cut(s)	171, 228, 358, 498, 932
HpySE526I	ACGT	1 cut(s)	269
Hsp92II	CATG	7 cut(s)	566, 653, 782, 957, 1363, 1407, 1414
HspAI	GCGC	1 cut(s)	1309
Kzo9I	GATC	9 cut(s)	189, 397, 417, 460, 505, 542, 565, 612, 1003
LguI	GCTCTTC	1 cut(s)	1305
LmnI	GCTCC	5 cut(s)	889, 937, 1017, 1078, 1181
Lsp1109I	GCAGC	8 cut(s)	403, 629, 638, 673, 1048, 1093, 1194, 1197
LweI	GCATC	1 cut(s)	1416
MabI	ACCWGGT	1 cut(s)	372
MaeI	CTAG	4 cut(s)	101, 449, 789, 1350
MaeII	ACGT	1 cut(s)	269
MaeIII	GTNAC	3 cut(s)	353, 1114, 1280
MalI	GATC	9 cut(s)	191, 399, 419, 462, 507, 544, 567, 614, 1005
MboI	GATC	9 cut(s)	189, 397, 417, 460, 505, 542, 565, 612, 1003
MboII	GAAGA	8 cut(s)	70, 560, 622, 994, 1185, 1240, 1247, 1292
MflI	RGATCY	2 cut(s)	542, 1003
MhlI	GDGCHC	1 cut(s)	820
MlsI	TGGCCA	1 cut(s)	749
MluNI	TGGCCA	1 cut(s)	749
MlyI	GAGTC	2 cut(s)	769, 1195
MmeI	TCCRAC	1 cut(s)	252
Mox20I	TGGCCA	1 cut(s)	749
Mph1103I	ATGCAT	1 cut(s)	1409
MroXI	GAANNNNTTC	1 cut(s)	986
MscI	TGGCCA	1 cut(s)	749
MseI	TTAA	1 cut(s)	852
MslI	CAYNNNNRTG	2 cut(s)	561, 1412
Msp20I	TGGCCA	1 cut(s)	749
MspA1I	CMGCKG	2 cut(s)	620, 844
MspI	CCGG	1 cut(s)	979
MspR9I	CCNGG	2 cut(s)	374, 1440
MvaI	CCWGG	2 cut(s)	374, 1440
MvnI	CGCG	1 cut(s)	681
MwoI	GCNNNNNNNGC	5 cut(s)	408, 623, 626, 1061, 1090
NdeI	CATATG	1 cut(s)	805
NdeII	GATC	9 cut(s)	189, 397, 417, 460, 505, 542, 565, 612, 1003
NlaIII	CATG	7 cut(s)	566, 653, 782, 957, 1363, 1407, 1414
NlaIV	GGNNCC	2 cut(s)	1005, 1437
NmuCI	GTSAC	2 cut(s)	353, 1114
NruI	TCGCGA	1 cut(s)	681
NsbI	TGCGCA	1 cut(s)	1310
NsiI	ATGCAT	1 cut(s)	1409
NspI	RCATGY	1 cut(s)	1407
PciSI	GCTCTTC	1 cut(s)	1305
PdmI	GAANNNNTTC	1 cut(s)	986
PfeI	GAWTC	2 cut(s)	333, 469
Ple19I	CGATCG	1 cut(s)	192
PleI	GAGTC	2 cut(s)	769, 1194
PpsI	GAGTC	2 cut(s)	769, 1194
Psp6I	CCWGG	2 cut(s)	372, 1438
PspGI	CCWGG	2 cut(s)	372, 1438
PspN4I	GGNNCC	2 cut(s)	1005, 1437
PspPI	GGNCC	1 cut(s)	758
PstI	CTGCAG	1 cut(s)	1289
PsuI	RGATCY	2 cut(s)	542, 1003
PvuI	CGATCG	1 cut(s)	192
PvuII	CAGCTG	2 cut(s)	620, 844
RruI	TCGCGA	1 cut(s)	681
RsaI	GTAC	4 cut(s)	812, 1170, 1272, 1347
RsaNI	GTAC	4 cut(s)	811, 1169, 1271, 1346
RseI	CAYNNNNRTG	2 cut(s)	561, 1412
SapI	GCTCTTC	1 cut(s)	1305
SaqAI	TTAA	1 cut(s)	852
Sau3AI	GATC	9 cut(s)	189, 397, 417, 460, 505, 542, 565, 612, 1003
Sau96I	GGNCC	1 cut(s)	758
SchI	GAGTC	2 cut(s)	769, 1195
ScrFI	CCNGG	2 cut(s)	374, 1440
SduI	GDGCHC	1 cut(s)	820
SexAI	ACCWGGT	1 cut(s)	372
SfaNI	GCATC	1 cut(s)	1416
SfcI	CTRYAG	2 cut(s)	82, 1285
SinI	GGWCC	1 cut(s)	758
SmiMI	CAYNNNNRTG	2 cut(s)	561, 1412
SsiI	CCGC	4 cut(s)	253, 579, 1093, 1264
SspI	AATATT	1 cut(s)	1224
SspMI	CTAG	4 cut(s)	101, 449, 789, 1350
StyD4I	CCNGG	2 cut(s)	372, 1438
StyI	CCWWGG	1 cut(s)	412
TaaI	ACNGT	3 cut(s)	83, 815, 1019
TaiI	ACGT	1 cut(s)	272
TaqI	TCGA	2 cut(s)	188, 192
TatI	WGTACW	2 cut(s)	1168, 1270
TauI	GCSGC	2 cut(s)	256, 1096
TfiI	GAWTC	2 cut(s)	333, 469
Tru1I	TTAA	1 cut(s)	852
Tru9I	TTAA	1 cut(s)	852
TseFI	GTSAC	2 cut(s)	353, 1114
TseI	GCWGC	8 cut(s)	391, 617, 626, 661, 1061, 1081, 1207, 1210
Tsp45I	GTSAC	2 cut(s)	353, 1114
TspDTI	ATGAA	2 cut(s)	540, 623
TspGWI	ACGGA	1 cut(s)	996
VneI	GTGCAC	1 cut(s)	816
VpaK11BI	GGWCC	1 cut(s)	758
XapI	RAATTY	5 cut(s)	25, 118, 425, 986, 1238
XbaI	TCTAGA	1 cut(s)	100
XceI	RCATGY	1 cut(s)	1407
XmnI	GAANNNNTTC	1 cut(s)	986
XspI	CTAG	4 cut(s)	101, 449, 789, 1350
Zsp2I	ATGCAT	1 cut(s)	1409

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.