RLG00000035693

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017026
Physical Location & Seq
Reverse (-)
70319561 .. 70323494
3934 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035693

Sequence Viewer

Length: 828 bp
ATGGCTGGCCGGGCCGTGCGACGAGTCCTGTCTGCGGCGGCAAAAGAAGTTACGGAAAGTGTTGTAGGGGTGGATCTGGCCGATTCCAGGGTACTATTCCTGGCAAAAGCTAGCATTGCTACCTTCTTCTCATGGATAAGATTTTTTGTTGTTGTTTTTGTGAGGGTGAAGAGGGCTTTCAATCTCAATTTAAGGAAAGTGTTTGTGATGATTGATGCCAATATAGACAAAGTTTTCTTTACAAGGAAGAGCCTTCTGATTGGAGCCAACTTTGCTTATGCTGCATCCGGTTATGATGAAAAGGCAGCAAGCTTGAATCATGCAATCTCTTTGCCCCAACAGTTACAATACTTCAAGGAATACGAGGGTAAGCTAAACAAGGTTGCAGGTAGTAAGAAAGCAGCAACAATCATCAATGGTGCACTATACTTATTGAGTGCTGGCCGTAGTGACTTTGTTCAGAACTACTATGTCAATCCTTTTGTTAACGAACTCTACACTCCCGACCAGTACGGCGACATCCTCGTTTGTGCTTTCACACACTTTATTACGGATGTGTATGGTTTGGGAGCAAGGAAAATAGATGCAAAAAGGCTGATTGGTAGGAAATTCAGTGATGCATGTGTCAATAATGACATAAAGCTATGGCCTTTCAAGGTGCGTAGAGATATGTGGAACAGGCCAAAGATTGTGATCAACTACAAGGGTAAGGAGAAGCAGTTTTCTGCTAAGGAGATATCTTCCATGGTTCTCTATATGATGTGTCAAATAGCTGAGGCCTACCTTGGATTGATGGTCAAAAATGCTGTTATAACTGTCCTCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

276

Amino Acids

31.0

Weight (kDa)

9.77

Isoelectric Point (pI)

26.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 194 - 273 5.4e-18 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 377
AciI CCGC 2 cut(s) 35, 38
AclWI GGATC 1 cut(s) 81
AcoI YGGCCR 3 cut(s) 7, 78, 442
AcsI RAATTY 1 cut(s) 608
AfaI GTAC 2 cut(s) 93, 512
AfiI CCNNNNNNNGG 2 cut(s) 34, 87
AgsI TTSAA 4 cut(s) 181, 316, 355, 655
AjnI CCWGG 2 cut(s) 86, 99
AluBI AGCT 5 cut(s) 110, 312, 373, 643, 773
AluI AGCT 5 cut(s) 110, 312, 373, 643, 773
Alw21I GWGCWC 1 cut(s) 424
Alw44I GTGCAC 1 cut(s) 420
AlwI GGATC 1 cut(s) 81
AoxI GGCC 7 cut(s) 7, 12, 78, 442, 647, 680, 777
ApaLI GTGCAC 1 cut(s) 420
ApeKI GCWGC 3 cut(s) 281, 305, 401
ApoI RAATTY 1 cut(s) 608
AspS9I GGNCC 1 cut(s) 12
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 178
AsuNHI GCTAGC 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 424
BbvCI CCTCAGC 1 cut(s) 774
BbvI GCAGC 3 cut(s) 268, 317, 413
BccI CCATC 1 cut(s) 787
BceAI ACGGC 2 cut(s) 429, 529
BciT130I CCWGG 2 cut(s) 88, 101
BclI TGATCA 1 cut(s) 693
BcnI CCSGG 1 cut(s) 11
BfaI CTAG 1 cut(s) 111
BfuAI ACCTGC 1 cut(s) 377
BisI GCNGC 5 cut(s) 36, 39, 282, 306, 402
BlsI GCNGC 5 cut(s) 37, 40, 283, 307, 403
Bme1390I CCNGG 3 cut(s) 11, 88, 101
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 3 cut(s) 11, 88, 101
BmsI GCATC 4 cut(s) 205, 293, 574, 607
BmtI GCTAGC 1 cut(s) 114
Bpu10I CCTNAGC 2 cut(s) 729, 774
BpuMI CCSGG 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 692
BsaJI CCNNGG 3 cut(s) 87, 744, 784
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 704, 734
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 87
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 1 cut(s) 114
Bse8I GATNNNNATC 1 cut(s) 692
BseBI CCWGG 2 cut(s) 88, 101
BseDI CCNNGG 3 cut(s) 87, 744, 784
BseGI GGATG 3 cut(s) 284, 519, 559
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 2 cut(s) 34, 87
BseMI GCAATG 1 cut(s) 114
BseMII CTCAG 1 cut(s) 765
BseNI ACTGG 1 cut(s) 508
BseSI GKGCMC 1 cut(s) 424
BseXI GCAGC 3 cut(s) 268, 317, 413
BshFI GGCC 7 cut(s) 9, 14, 80, 444, 649, 682, 779
BsiHKAI GWGCWC 1 cut(s) 424
BsiSI CCGG 2 cut(s) 10, 288
BslI CCNNNNNNNGG 2 cut(s) 34, 87
BsnI GGCC 7 cut(s) 9, 14, 80, 444, 649, 682, 779
Bsp1286I GDGCHC 1 cut(s) 424
Bsp143I GATC 2 cut(s) 73, 693
Bsp19I CCATGG 1 cut(s) 744
BspACI CCGC 2 cut(s) 35, 38
BspANI GGCC 7 cut(s) 9, 14, 80, 444, 649, 682, 779
BspCNI CTCAG 1 cut(s) 766
BspLI GGNNCC 1 cut(s) 265
BspMI ACCTGC 1 cut(s) 377
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 81
BspQI GCTCTTC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 114
BsrI ACTGG 1 cut(s) 508
BssECI CCNNGG 3 cut(s) 87, 744, 784
BssMI GATC 2 cut(s) 73, 693
BssT1I CCWWGG 2 cut(s) 744, 784
Bst2UI CCWGG 2 cut(s) 88, 101
Bst4CI ACNGT 2 cut(s) 342, 817
Bst6I CTCTTC 2 cut(s) 164, 242
BstC8I GCNNGC 4 cut(s) 7, 112, 310, 442
BstDEI CTNAG 2 cut(s) 729, 774
BstDSI CCRYGG 1 cut(s) 744
BstF5I GGATG 3 cut(s) 284, 519, 559
BstKTI GATC 2 cut(s) 76, 696
BstMBI GATC 2 cut(s) 73, 693
BstMWI GCNNNNNNNGC 4 cut(s) 11, 116, 272, 281
BstNI CCWGG 2 cut(s) 88, 101
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 3 cut(s) 9, 86, 99
BstSLI GKGCMC 1 cut(s) 424
BstV1I GCAGC 3 cut(s) 268, 317, 413
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 7 cut(s) 9, 14, 80, 444, 649, 682, 779
BtgI CCRYGG 1 cut(s) 744
BtsCI GGATG 3 cut(s) 284, 519, 559
BtsIMutI CAGTG 1 cut(s) 619
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 4 cut(s) 7, 112, 310, 442
Cfr13I GGNCC 1 cut(s) 12
Csp6I GTAC 2 cut(s) 92, 511
CviAII CATG 4 cut(s) 132, 320, 621, 745
CviQI GTAC 2 cut(s) 92, 511
DdeI CTNAG 2 cut(s) 729, 774
DpnI GATC 2 cut(s) 75, 695
DpnII GATC 2 cut(s) 73, 693
EaeI YGGCCR 3 cut(s) 7, 78, 442
Eam1104I CTCTTC 2 cut(s) 164, 242
EarI CTCTTC 2 cut(s) 164, 242
Eco130I CCWWGG 2 cut(s) 744, 784
Eco147I AGGCCT 1 cut(s) 779
Eco32I GATATC 1 cut(s) 738
EcoRII CCWGG 2 cut(s) 86, 99
EcoRV GATATC 1 cut(s) 738
EcoT14I CCWWGG 2 cut(s) 744, 784
EcoT22I ATGCAT 1 cut(s) 622
ErhI CCWWGG 2 cut(s) 744, 784
FaeI CATG 4 cut(s) 135, 323, 624, 748
FatI CATG 4 cut(s) 131, 319, 620, 744
FbaI TGATCA 1 cut(s) 693
Fnu4HI GCNGC 5 cut(s) 36, 39, 282, 306, 402
FokI GGATG 3 cut(s) 271, 506, 566
Fsp4HI GCNGC 5 cut(s) 36, 39, 282, 306, 402
FspBI CTAG 1 cut(s) 111
GluI GCNGC 5 cut(s) 36, 39, 282, 306, 402
HaeIII GGCC 7 cut(s) 9, 14, 80, 444, 649, 682, 779
HapII CCGG 2 cut(s) 10, 288
Hin1II CATG 4 cut(s) 135, 323, 624, 748
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 1 cut(s) 310
HinfI GANTC 3 cut(s) 24, 83, 316
HpaI GTTAAC 1 cut(s) 487
HpaII CCGG 2 cut(s) 10, 288
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 2 cut(s) 422, 487
Hpy188I TCNGA 2 cut(s) 258, 462
Hpy188III TCNNGA 1 cut(s) 503
Hpy8I GTNNAC 2 cut(s) 422, 487
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 2 cut(s) 133, 263
HpyCH4III ACNGT 2 cut(s) 342, 817
HpyCH4V TGCA 6 cut(s) 284, 323, 386, 422, 587, 620
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 116, 272, 281
HpyF3I CTNAG 2 cut(s) 729, 774
Hsp92II CATG 4 cut(s) 135, 323, 624, 748
Ksp22I TGATCA 1 cut(s) 693
KspAI GTTAAC 1 cut(s) 487
Kzo9I GATC 2 cut(s) 73, 693
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 2 cut(s) 263, 569
Lsp1109I GCAGC 3 cut(s) 268, 317, 413
LweI GCATC 4 cut(s) 205, 293, 574, 607
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 3 cut(s) 49, 342, 449
MalI GATC 2 cut(s) 75, 695
MboI GATC 2 cut(s) 73, 693
MboII GAAGA 4 cut(s) 118, 181, 259, 732
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 424
MluCI AATT 2 cut(s) 187, 608
MlyI GAGTC 1 cut(s) 33
MnlI CCTC 5 cut(s) 156, 165, 358, 533, 769
Mph1103I ATGCAT 1 cut(s) 622
MseI TTAA 2 cut(s) 191, 486
MspI CCGG 2 cut(s) 10, 288
MspR9I CCNGG 3 cut(s) 11, 88, 101
MvaI CCWGG 2 cut(s) 88, 101
MwoI GCNNNNNNNGC 4 cut(s) 11, 116, 272, 281
NciI CCSGG 1 cut(s) 11
NcoI CCATGG 1 cut(s) 744
NdeII GATC 2 cut(s) 73, 693
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 4 cut(s) 135, 323, 624, 748
NlaIV GGNNCC 1 cut(s) 265
NmuCI GTSAC 1 cut(s) 449
NsiI ATGCAT 1 cut(s) 622
NspI RCATGY 1 cut(s) 624
PceI AGGCCT 1 cut(s) 779
PciSI GCTCTTC 1 cut(s) 242
PfeI GAWTC 2 cut(s) 83, 316
PkrI GCNGC 5 cut(s) 37, 40, 283, 307, 403
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PsiI TTATAA 1 cut(s) 812
Psp6I CCWGG 2 cut(s) 86, 99
PspGI CCWGG 2 cut(s) 86, 99
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 1 cut(s) 12
PsuI RGATCY 1 cut(s) 73
RsaI GTAC 2 cut(s) 93, 512
RsaNI GTAC 2 cut(s) 92, 511
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 2 cut(s) 191, 486
SatI GCNGC 5 cut(s) 36, 39, 282, 306, 402
Sau3AI GATC 2 cut(s) 73, 693
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 3 cut(s) 11, 88, 101
SduI GDGCHC 1 cut(s) 424
SfaNI GCATC 4 cut(s) 205, 293, 574, 607
Sse9I AATT 2 cut(s) 187, 608
SseBI AGGCCT 1 cut(s) 779
SsiI CCGC 2 cut(s) 35, 38
SspMI CTAG 1 cut(s) 111
StuI AGGCCT 1 cut(s) 779
StyD4I CCNGG 3 cut(s) 9, 86, 99
StyI CCWWGG 2 cut(s) 744, 784
TaaI ACNGT 2 cut(s) 342, 817
TasI AATT 2 cut(s) 187, 608
TauI GCSGC 2 cut(s) 38, 41
TfiI GAWTC 2 cut(s) 83, 316
Tru1I TTAA 2 cut(s) 191, 486
Tru9I TTAA 2 cut(s) 191, 486
TscAI CASTG 1 cut(s) 619
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 3 cut(s) 281, 305, 401
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 1 cut(s) 312
TspGWI ACGGA 2 cut(s) 68, 566
TspRI CASTG 1 cut(s) 619
VneI GTGCAC 1 cut(s) 420
XapI RAATTY 1 cut(s) 608
XceI RCATGY 1 cut(s) 624
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.