Rh4CG006500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0017026
Physical Location & Seq
Forward (+)
1258939 .. 1261379
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG006500.1

Sequence Viewer

Length: 339 bp
ATGGCTGGCCGGGCCGTGCGACGAGTCCTGTCTGCGGCGGCAAAAGAAGTTACGGAAAGTGTTGTAGGGGTGGATCTGGCCGATTCCAGGGTACTATTCCTGGCAAAAGCTAGCATTGCTACCTTCTTCTCATGGATAAGATTTTTTGTTGTTGTTTTTGTGAGGGTGAAGAGGGCTTTCAATCTCAATTTAAGGAAAGTGTTTGTGATGATTGATGCCAATATAGACAAAGTTTTCTTTACAAGGAAGAACCTTCTGATTGGAGCCAACTTTGCTTATGCTGCATCCGGTTATGATGAAAAGGCAGCAAGCTTGAATCATGCAATCTCTTTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

112

Amino Acids

12.4

Weight (kDa)

10.47

Isoelectric Point (pI)

29.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 35, 38
AclWI GGATC 1 cut(s) 81
AcoI YGGCCR 2 cut(s) 7, 78
AfaI GTAC 1 cut(s) 93
AfiI CCNNNNNNNGG 2 cut(s) 34, 87
AgsI TTSAA 2 cut(s) 181, 316
AjnI CCWGG 2 cut(s) 86, 99
AluBI AGCT 2 cut(s) 110, 312
AluI AGCT 2 cut(s) 110, 312
AlwI GGATC 1 cut(s) 81
AoxI GGCC 3 cut(s) 7, 12, 78
ApeKI GCWGC 2 cut(s) 281, 305
AspS9I GGNCC 1 cut(s) 12
AsuC2I CCSGG 1 cut(s) 11
AsuHPI GGTGA 1 cut(s) 178
AsuNHI GCTAGC 1 cut(s) 110
BbvI GCAGC 2 cut(s) 268, 317
BciT130I CCWGG 2 cut(s) 88, 101
BcnI CCSGG 1 cut(s) 11
BfaI CTAG 1 cut(s) 111
BisI GCNGC 4 cut(s) 36, 39, 282, 306
BlsI GCNGC 4 cut(s) 37, 40, 283, 307
Bme1390I CCNGG 3 cut(s) 11, 88, 101
BmgT120I GGNCC 1 cut(s) 12
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 3 cut(s) 11, 88, 101
BmsI GCATC 2 cut(s) 205, 293
BmtI GCTAGC 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 87
Bse3DI GCAATG 1 cut(s) 114
BseBI CCWGG 2 cut(s) 88, 101
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 284
BseLI CCNNNNNNNGG 2 cut(s) 34, 87
BseMI GCAATG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 268, 317
BshFI GGCC 3 cut(s) 9, 14, 80
BsiSI CCGG 2 cut(s) 10, 288
BslI CCNNNNNNNGG 2 cut(s) 34, 87
BsnI GGCC 3 cut(s) 9, 14, 80
Bsp143I GATC 1 cut(s) 73
BspACI CCGC 2 cut(s) 35, 38
BspANI GGCC 3 cut(s) 9, 14, 80
BspLI GGNNCC 1 cut(s) 265
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 1 cut(s) 81
BsrDI GCAATG 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 1 cut(s) 73
Bst2UI CCWGG 2 cut(s) 88, 101
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 3 cut(s) 7, 112, 310
BstF5I GGATG 1 cut(s) 284
BstKTI GATC 1 cut(s) 76
BstMBI GATC 1 cut(s) 73
BstMWI GCNNNNNNNGC 4 cut(s) 11, 116, 272, 281
BstNI CCWGG 2 cut(s) 88, 101
BstSCI CCNGG 3 cut(s) 9, 86, 99
BstV1I GCAGC 2 cut(s) 268, 317
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 3 cut(s) 9, 14, 80
BtsCI GGATG 1 cut(s) 284
Cac8I GCNNGC 3 cut(s) 7, 112, 310
Cfr13I GGNCC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 92
CviAII CATG 2 cut(s) 132, 320
CviJI RGCY 8 cut(s) 5, 9, 14, 80, 110, 176, 266, 312
CviKI_1 RGCY 8 cut(s) 5, 9, 14, 80, 110, 176, 266, 312
CviQI GTAC 1 cut(s) 92
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
EaeI YGGCCR 2 cut(s) 7, 78
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 86, 99
FaeI CATG 2 cut(s) 135, 323
FaiI YATR 5 cut(s) 133, 224, 279, 294, 321
FatI CATG 2 cut(s) 131, 319
Fnu4HI GCNGC 4 cut(s) 36, 39, 282, 306
FokI GGATG 1 cut(s) 271
Fsp4HI GCNGC 4 cut(s) 36, 39, 282, 306
FspBI CTAG 1 cut(s) 111
GluI GCNGC 4 cut(s) 36, 39, 282, 306
HaeIII GGCC 3 cut(s) 9, 14, 80
HapII CCGG 2 cut(s) 10, 288
Hin1II CATG 2 cut(s) 135, 323
HindIII AAGCTT 1 cut(s) 310
HinfI GANTC 3 cut(s) 24, 83, 316
HpaII CCGG 2 cut(s) 10, 288
HphI GGTGA 1 cut(s) 178
Hpy188I TCNGA 1 cut(s) 258
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 2 cut(s) 133, 263
HpyCH4V TGCA 2 cut(s) 284, 323
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 116, 272, 281
Hsp92II CATG 2 cut(s) 135, 323
Kzo9I GATC 1 cut(s) 73
LmnI GCTCC 1 cut(s) 263
LpnPI CCDG 8 cut(s) 23, 41, 62, 73, 86, 100, 113, 301
Lsp1109I GCAGC 2 cut(s) 268, 317
LweI GCATC 2 cut(s) 205, 293
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 49
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 3 cut(s) 118, 181, 259
MflI RGATCY 1 cut(s) 73
MluCI AATT 1 cut(s) 187
MlyI GAGTC 1 cut(s) 33
MnlI CCTC 2 cut(s) 156, 165
MseI TTAA 1 cut(s) 191
MspI CCGG 2 cut(s) 10, 288
MspR9I CCNGG 3 cut(s) 11, 88, 101
MvaI CCWGG 2 cut(s) 88, 101
MwoI GCNNNNNNNGC 4 cut(s) 11, 116, 272, 281
NciI CCSGG 1 cut(s) 11
NdeII GATC 1 cut(s) 73
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 2 cut(s) 135, 323
NlaIV GGNNCC 1 cut(s) 265
PfeI GAWTC 2 cut(s) 83, 316
PkrI GCNGC 4 cut(s) 37, 40, 283, 307
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 86, 99
PspGI CCWGG 2 cut(s) 86, 99
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 1 cut(s) 12
PsuI RGATCY 1 cut(s) 73
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
SaqAI TTAA 1 cut(s) 191
SatI GCNGC 4 cut(s) 36, 39, 282, 306
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 1 cut(s) 12
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 3 cut(s) 11, 88, 101
SetI ASST 4 cut(s) 112, 125, 255, 314
SfaNI GCATC 2 cut(s) 205, 293
Sse9I AATT 1 cut(s) 187
SsiI CCGC 2 cut(s) 35, 38
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 3 cut(s) 9, 86, 99
TasI AATT 1 cut(s) 187
TauI GCSGC 2 cut(s) 38, 41
TfiI GAWTC 2 cut(s) 83, 316
Tru1I TTAA 1 cut(s) 191
Tru9I TTAA 1 cut(s) 191
TseI GCWGC 2 cut(s) 281, 305
TspDTI ATGAA 1 cut(s) 312
TspGWI ACGGA 1 cut(s) 68
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.