Rw0G013710

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00615
OG0017026
Physical Location & Seq
Reverse (-)
18790 .. 20716
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G013710.1

Sequence Viewer

Length: 459 bp
ATGGTTGTGACAAAAGGAGATGAAGAAGGGAAGAAGATTGCAGCAATGGTGAGGGAGATTGGGCCTTCTGAGCTTGTTCTTGGACTCCATGATCACAAGAAGAACCTTCTGATTGGAGCCAACTTTGCTTATGCTGCATCCGGTTATGATGAAAAGGCAGCAAGCTTGAATCATGCAATCTCTTTGCCCCAACAGTTACAATACTTCAAGGAATACGAGGGTAAGCTAAACAAGGTTGCAGGTAGTAAGAAAGCAGCAACAATCATCAATGGTGCACTATACTTATTGAGTGCTGGCCATAGTGACTTCGTTCAGAACTACTATGTCAATCCTTTTGTTAACGAACTCTACACTCCTGACCAGTACGGCGACATCCTCGTTTGTGCTTTCACACGCTTTATTACGGATGTGTATGGTTTGGGAGCAAGGAAAATAGGTGTGACATCAAGATTCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

152

Amino Acids

16.68

Weight (kDa)

7.8

Isoelectric Point (pI)

17.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 230
AcoI YGGCCR 1 cut(s) 295
AfaI GTAC 1 cut(s) 365
AgsI TTSAA 2 cut(s) 169, 208
AluBI AGCT 3 cut(s) 73, 165, 226
AluI AGCT 3 cut(s) 73, 165, 226
Alw21I GWGCWC 1 cut(s) 277
Alw44I GTGCAC 1 cut(s) 273
AoxI GGCC 2 cut(s) 62, 295
ApaLI GTGCAC 1 cut(s) 273
ApeKI GCWGC 4 cut(s) 41, 134, 158, 254
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 61, 445
BaeGI GKGCMC 1 cut(s) 277
BalI TGGCCA 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 277
BbvI GCAGC 4 cut(s) 53, 121, 170, 266
BceAI ACGGC 1 cut(s) 382
BclI TGATCA 1 cut(s) 91
BfuAI ACCTGC 1 cut(s) 230
BisI GCNGC 4 cut(s) 42, 135, 159, 255
BlsI GCNGC 4 cut(s) 43, 136, 160, 256
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 118
BmsI GCATC 1 cut(s) 146
BsaWI WCCGGW 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 1 cut(s) 51
BseGI GGATG 3 cut(s) 137, 372, 412
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 361
BseSI GKGCMC 1 cut(s) 277
BseXI GCAGC 4 cut(s) 53, 121, 170, 266
BshFI GGCC 2 cut(s) 64, 297
BsiHKAI GWGCWC 1 cut(s) 277
BsiSI CCGG 1 cut(s) 141
BsnI GGCC 2 cut(s) 64, 297
Bsp1286I GDGCHC 1 cut(s) 277
Bsp143I GATC 1 cut(s) 91
BspANI GGCC 2 cut(s) 64, 297
BspCNI CTCAG 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 118
BspMI ACCTGC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 1 cut(s) 361
BssMI GATC 1 cut(s) 91
Bst4CI ACNGT 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 163, 295
BstDEI CTNAG 1 cut(s) 69
BstF5I GGATG 3 cut(s) 137, 372, 412
BstKTI GATC 1 cut(s) 94
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 3 cut(s) 70, 125, 134
BstSLI GKGCMC 1 cut(s) 277
BstV1I GCAGC 4 cut(s) 53, 121, 170, 266
BsuRI GGCC 2 cut(s) 64, 297
BtsCI GGATG 3 cut(s) 137, 372, 412
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 2 cut(s) 163, 295
Cfr13I GGNCC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 364
CviAII CATG 2 cut(s) 89, 173
CviJI RGCY 6 cut(s) 64, 73, 119, 165, 226, 297
CviKI_1 RGCY 6 cut(s) 64, 73, 119, 165, 226, 297
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 1 cut(s) 69
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 295
FaeI CATG 2 cut(s) 92, 176
FaiI YATR 8 cut(s) 90, 132, 147, 174, 280, 300, 324, 414
FatI CATG 2 cut(s) 88, 172
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 4 cut(s) 42, 135, 159, 255
FokI GGATG 3 cut(s) 124, 359, 419
Fsp4HI GCNGC 4 cut(s) 42, 135, 159, 255
GluI GCNGC 4 cut(s) 42, 135, 159, 255
HaeIII GGCC 2 cut(s) 64, 297
HapII CCGG 1 cut(s) 141
Hin1II CATG 2 cut(s) 92, 176
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 3 cut(s) 84, 169, 450
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 1 cut(s) 141
HphI GGTGA 2 cut(s) 61, 445
Hpy166II GTNNAC 2 cut(s) 275, 340
Hpy188I TCNGA 3 cut(s) 70, 111, 315
Hpy188III TCNNGA 2 cut(s) 356, 447
Hpy8I GTNNAC 2 cut(s) 275, 340
HpyAV CCTTC 3 cut(s) 20, 75, 116
HpyCH4III ACNGT 1 cut(s) 195
HpyCH4V TGCA 5 cut(s) 41, 137, 176, 239, 275
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 125, 134
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 2 cut(s) 92, 176
Ksp22I TGATCA 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 1 cut(s) 91
LmnI GCTCC 2 cut(s) 116, 422
LpnPI CCDG 5 cut(s) 154, 225, 279, 369, 374
Lsp1109I GCAGC 4 cut(s) 53, 121, 170, 266
LweI GCATC 1 cut(s) 146
MaeIII GTNAC 4 cut(s) 7, 195, 302, 439
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 4 cut(s) 35, 43, 46, 112
MhlI GDGCHC 1 cut(s) 277
MlsI TGGCCA 1 cut(s) 297
MluNI TGGCCA 1 cut(s) 297
MlyI GAGTC 1 cut(s) 78
MnlI CCTC 3 cut(s) 45, 211, 386
Mox20I TGGCCA 1 cut(s) 297
MscI TGGCCA 1 cut(s) 297
MseI TTAA 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 297
MspI CCGG 1 cut(s) 141
MwoI GCNNNNNNNGC 3 cut(s) 70, 125, 134
NdeII GATC 1 cut(s) 91
NlaIII CATG 2 cut(s) 92, 176
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 3 cut(s) 7, 302, 439
PfeI GAWTC 2 cut(s) 169, 450
PkrI GCNGC 4 cut(s) 43, 136, 160, 256
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 62
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 4 cut(s) 42, 135, 159, 255
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 62
SchI GAGTC 1 cut(s) 78
SduI GDGCHC 1 cut(s) 277
SetI ASST 8 cut(s) 75, 108, 167, 228, 237, 244, 439, 458
SfaNI GCATC 1 cut(s) 146
TaaI ACNGT 1 cut(s) 195
TfiI GAWTC 2 cut(s) 169, 450
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TseFI GTSAC 3 cut(s) 7, 302, 439
TseI GCWGC 4 cut(s) 41, 134, 158, 254
Tsp45I GTSAC 3 cut(s) 7, 302, 439
TspDTI ATGAA 2 cut(s) 36, 165
TspGWI ACGGA 1 cut(s) 419
VneI GTGCAC 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.