Rmu_sc0001496.1_g000020

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001496.1
OG0017026
Physical Location & Seq
Forward (+)
64170 .. 65952
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001496.1_g000020.1.cds

Sequence Viewer

Length: 507 bp
atggttgtgacaaaaggagatgaagaagggaagaagattgcagcaatggtgagggagattgggccttctgagcttgttcttggactccatgatcacagttttctttacaatgtttttgtttatcatctggtttatgcatcagggaagaaccttctgattggagccaactttgcttatgctgcatccggttatgatgaaaaggcagcaagcttgaatcgtgcaatctctttgccccaacagttacaatacttcaaggaatacgagggtaagctaaacaaggttgcaggtagtaagaaagcagcaacaatcatcaatggtgcactatacttattgagtgctggccatagtgacttcattcagaactactatgtcaatccttttgttaacgaactctacactcctgaccagtacggcaacatcctcgtttgtgctttcacacgctttattacagtaccttctcaaattgccacttctgttaatcctactttaatgaagaatctactttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

168

Amino Acids

18.54

Weight (kDa)

7.8

Isoelectric Point (pI)

22.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 275
AcoI YGGCCR 1 cut(s) 340
AfaI GTAC 2 cut(s) 410, 453
AgsI TTSAA 2 cut(s) 214, 253
AluBI AGCT 3 cut(s) 73, 210, 271
AluI AGCT 3 cut(s) 73, 210, 271
Alw21I GWGCWC 1 cut(s) 322
Alw44I GTGCAC 1 cut(s) 318
AoxI GGCC 2 cut(s) 62, 340
ApaLI GTGCAC 1 cut(s) 318
ApeKI GCWGC 4 cut(s) 41, 179, 203, 299
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 322
BalI TGGCCA 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 322
BbvI GCAGC 4 cut(s) 53, 166, 215, 311
BceAI ACGGC 1 cut(s) 427
BcgI CGANNNNNNTGC 2 cut(s) 403, 437
BclI TGATCA 1 cut(s) 91
BfuAI ACCTGC 1 cut(s) 275
BisI GCNGC 4 cut(s) 42, 180, 204, 300
BlsI GCNGC 4 cut(s) 43, 181, 205, 301
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 163
BmsI GCATC 2 cut(s) 146, 191
BsaWI WCCGGW 1 cut(s) 185
Bse1I ACTGG 1 cut(s) 406
Bse3DI GCAATG 1 cut(s) 51
BseGI GGATG 2 cut(s) 182, 417
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 406
BseSI GKGCMC 1 cut(s) 322
BseXI GCAGC 4 cut(s) 53, 166, 215, 311
BshFI GGCC 2 cut(s) 64, 342
BsiHKAI GWGCWC 1 cut(s) 322
BsiSI CCGG 1 cut(s) 186
BsnI GGCC 2 cut(s) 64, 342
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 1 cut(s) 91
BspANI GGCC 2 cut(s) 64, 342
BspCNI CTCAG 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 163
BspMI ACCTGC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 1 cut(s) 406
BssMI GATC 1 cut(s) 91
Bst4CI ACNGT 3 cut(s) 98, 240, 451
BstC8I GCNNGC 2 cut(s) 208, 340
BstDEI CTNAG 1 cut(s) 69
BstF5I GGATG 2 cut(s) 182, 417
BstKTI GATC 1 cut(s) 94
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 3 cut(s) 70, 170, 179
BstSLI GKGCMC 1 cut(s) 322
BstV1I GCAGC 4 cut(s) 53, 166, 215, 311
BsuRI GGCC 2 cut(s) 64, 342
BtsCI GGATG 2 cut(s) 182, 417
BveI ACCTGC 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 208, 340
Cfr13I GGNCC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 409, 452
CviAII CATG 1 cut(s) 89
CviJI RGCY 6 cut(s) 64, 73, 164, 210, 271, 342
CviKI_1 RGCY 6 cut(s) 64, 73, 164, 210, 271, 342
CviQI GTAC 2 cut(s) 409, 452
DdeI CTNAG 1 cut(s) 69
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 340
EcoT22I ATGCAT 1 cut(s) 139
FaeI CATG 1 cut(s) 92
FaiI YATR 7 cut(s) 90, 135, 177, 192, 325, 345, 369
FatI CATG 1 cut(s) 88
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 4 cut(s) 42, 180, 204, 300
FokI GGATG 2 cut(s) 169, 404
Fsp4HI GCNGC 4 cut(s) 42, 180, 204, 300
GluI GCNGC 4 cut(s) 42, 180, 204, 300
HaeIII GGCC 2 cut(s) 64, 342
HapII CCGG 1 cut(s) 186
Hin1II CATG 1 cut(s) 92
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HindIII AAGCTT 1 cut(s) 208
HinfI GANTC 3 cut(s) 84, 214, 496
HpaI GTTAAC 1 cut(s) 385
HpaII CCGG 1 cut(s) 186
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 320, 385
Hpy188I TCNGA 3 cut(s) 70, 156, 360
Hpy188III TCNNGA 1 cut(s) 401
Hpy8I GTNNAC 2 cut(s) 320, 385
HpyAV CCTTC 4 cut(s) 20, 75, 161, 465
HpyCH4III ACNGT 3 cut(s) 98, 240, 451
HpyCH4V TGCA 6 cut(s) 41, 137, 182, 221, 284, 320
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 170, 179
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 1 cut(s) 92
Ksp22I TGATCA 1 cut(s) 91
KspAI GTTAAC 1 cut(s) 385
Kzo9I GATC 1 cut(s) 91
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 7 cut(s) 113, 126, 199, 270, 324, 414, 419
Lsp1109I GCAGC 4 cut(s) 53, 166, 215, 311
LweI GCATC 2 cut(s) 146, 191
MaeIII GTNAC 3 cut(s) 7, 240, 347
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 5 cut(s) 35, 43, 46, 157, 505
MhlI GDGCHC 1 cut(s) 322
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 1 cut(s) 462
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 1 cut(s) 78
MnlI CCTC 3 cut(s) 45, 256, 431
Mox20I TGGCCA 1 cut(s) 342
Mph1103I ATGCAT 1 cut(s) 139
MscI TGGCCA 1 cut(s) 342
MseI TTAA 3 cut(s) 384, 477, 488
Msp20I TGGCCA 1 cut(s) 342
MspI CCGG 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 70, 170, 179
NdeII GATC 1 cut(s) 91
NlaIII CATG 1 cut(s) 92
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 2 cut(s) 7, 347
NsiI ATGCAT 1 cut(s) 139
PfeI GAWTC 2 cut(s) 214, 496
PkrI GCNGC 4 cut(s) 43, 181, 205, 301
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 1 cut(s) 62
RsaI GTAC 2 cut(s) 410, 453
RsaNI GTAC 2 cut(s) 409, 452
SaqAI TTAA 3 cut(s) 384, 477, 488
SatI GCNGC 4 cut(s) 42, 180, 204, 300
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 62
SchI GAGTC 1 cut(s) 78
SduI GDGCHC 1 cut(s) 322
SetI ASST 7 cut(s) 75, 153, 212, 273, 282, 289, 457
SfaNI GCATC 2 cut(s) 146, 191
Sse9I AATT 1 cut(s) 462
TaaI ACNGT 3 cut(s) 98, 240, 451
TasI AATT 1 cut(s) 462
TfiI GAWTC 2 cut(s) 214, 496
Tru1I TTAA 3 cut(s) 384, 477, 488
Tru9I TTAA 3 cut(s) 384, 477, 488
TseFI GTSAC 2 cut(s) 7, 347
TseI GCWGC 4 cut(s) 41, 179, 203, 299
Tsp45I GTSAC 2 cut(s) 7, 347
TspDTI ATGAA 4 cut(s) 36, 210, 343, 506
VneI GTGCAC 1 cut(s) 318
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.