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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 295 |
| AfeI | AGCGCT | 2 cut(s) | 63, 74 |
| AgsI | TTSAA | 3 cut(s) | 112, 119, 148 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 148, 215 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 60, 92 |
| AluBI | AGCT | 2 cut(s) | 176, 232 |
| AluI | AGCT | 2 cut(s) | 176, 232 |
| Alw26I | GTCTC | 1 cut(s) | 283 |
| Aor51HI | AGCGCT | 2 cut(s) | 63, 74 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 177 |
| AspLEI | GCGC | 3 cut(s) | 64, 75, 135 |
| BcoDI | GTCTC | 1 cut(s) | 283 |
| BfaI | CTAG | 1 cut(s) | 65 |
| BfoI | RGCGCY | 2 cut(s) | 65, 76 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 148, 215 |
| BmsI | GCATC | 1 cut(s) | 31 |
| BsaI | GGTCTC | 1 cut(s) | 283 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 14, 44 |
| Bse3DI | GCAATG | 1 cut(s) | 88 |
| BseMI | GCAATG | 1 cut(s) | 88 |
| BseMII | CTCAG | 1 cut(s) | 28 |
| BsmAI | GTCTC | 1 cut(s) | 283 |
| Bso31I | GGTCTC | 1 cut(s) | 283 |
| Bsp143I | GATC | 1 cut(s) | 33 |
| BspCNI | CTCAG | 1 cut(s) | 27 |
| BspQI | GCTCTTC | 2 cut(s) | 63, 183 |
| BspTNI | GGTCTC | 1 cut(s) | 283 |
| BsrDI | GCAATG | 1 cut(s) | 88 |
| BssMI | GATC | 1 cut(s) | 33 |
| Bst6I | CTCTTC | 2 cut(s) | 63, 183 |
| BstDEI | CTNAG | 2 cut(s) | 14, 297 |
| BstH2I | RGCGCY | 2 cut(s) | 65, 76 |
| BstHHI | GCGC | 3 cut(s) | 64, 75, 135 |
| BstKTI | GATC | 1 cut(s) | 36 |
| BstMAI | GTCTC | 1 cut(s) | 283 |
| BstMBI | GATC | 1 cut(s) | 33 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 70 |
| CfoI | GCGC | 3 cut(s) | 64, 75, 135 |
| Csp6I | GTAC | 1 cut(s) | 294 |
| CviAII | CATG | 2 cut(s) | 193, 227 |
| CviJI | RGCY | 2 cut(s) | 176, 232 |
| CviKI_1 | RGCY | 2 cut(s) | 176, 232 |
| CviQI | GTAC | 1 cut(s) | 294 |
| DdeI | CTNAG | 2 cut(s) | 14, 297 |
| DpnI | GATC | 1 cut(s) | 35 |
| DpnII | GATC | 1 cut(s) | 33 |
| DriI | GACNNNNNGTC | 2 cut(s) | 148, 215 |
| Eam1104I | CTCTTC | 2 cut(s) | 63, 183 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 148, 215 |
| EarI | CTCTTC | 2 cut(s) | 63, 183 |
| Eco31I | GGTCTC | 1 cut(s) | 283 |
| Eco47III | AGCGCT | 2 cut(s) | 63, 74 |
| FaeI | CATG | 2 cut(s) | 196, 230 |
| FaiI | YATR | 5 cut(s) | 27, 55, 165, 194, 228 |
| FatI | CATG | 2 cut(s) | 192, 226 |
| FspBI | CTAG | 1 cut(s) | 65 |
| GlaI | GCGC | 3 cut(s) | 63, 74, 134 |
| HaeII | RGCGCY | 2 cut(s) | 65, 76 |
| HhaI | GCGC | 3 cut(s) | 64, 75, 135 |
| Hin1II | CATG | 2 cut(s) | 196, 230 |
| Hin6I | GCGC | 3 cut(s) | 62, 73, 133 |
| HinP1I | GCGC | 3 cut(s) | 62, 73, 133 |
| HinfI | GANTC | 1 cut(s) | 92 |
| Hpy166II | GTNNAC | 2 cut(s) | 209, 252 |
| Hpy188I | TCNGA | 2 cut(s) | 17, 172 |
| Hpy8I | GTNNAC | 2 cut(s) | 209, 252 |
| HpyAV | CCTTC | 3 cut(s) | 106, 106, 265 |
| HpyCH4V | TGCA | 1 cut(s) | 44 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 70 |
| HpyF3I | CTNAG | 2 cut(s) | 14, 297 |
| Hsp92II | CATG | 2 cut(s) | 196, 230 |
| HspAI | GCGC | 3 cut(s) | 62, 73, 133 |
| Kzo9I | GATC | 1 cut(s) | 33 |
| LguI | GCTCTTC | 2 cut(s) | 63, 183 |
| LpnPI | CCDG | 2 cut(s) | 117, 228 |
| LweI | GCATC | 1 cut(s) | 31 |
| MaeI | CTAG | 1 cut(s) | 65 |
| MaeIII | GTNAC | 1 cut(s) | 217 |
| MalI | GATC | 1 cut(s) | 35 |
| MboI | GATC | 1 cut(s) | 33 |
| MboII | GAAGA | 2 cut(s) | 80, 170 |
| MfeI | CAATTG | 1 cut(s) | 270 |
| MluCI | AATT | 4 cut(s) | 127, 166, 234, 270 |
| MnlI | CCTC | 1 cut(s) | 110 |
| MroXI | GAANNNNTTC | 1 cut(s) | 177 |
| MunI | CAATTG | 1 cut(s) | 270 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 70 |
| NdeII | GATC | 1 cut(s) | 33 |
| NlaIII | CATG | 2 cut(s) | 196, 230 |
| NmuCI | GTSAC | 1 cut(s) | 217 |
| PciSI | GCTCTTC | 2 cut(s) | 63, 183 |
| PdmI | GAANNNNTTC | 1 cut(s) | 177 |
| PfeI | GAWTC | 1 cut(s) | 92 |
| RsaI | GTAC | 1 cut(s) | 295 |
| RsaNI | GTAC | 1 cut(s) | 294 |
| SapI | GCTCTTC | 2 cut(s) | 63, 183 |
| Sau3AI | GATC | 1 cut(s) | 33 |
| SetI | ASST | 6 cut(s) | 32, 117, 178, 217, 234, 257 |
| SfaNI | GCATC | 1 cut(s) | 31 |
| SgeI | CNNG | 9 cut(s) | 57, 77, 101, 116, 121, 160, 205, 227, 239 |
| Sse9I | AATT | 4 cut(s) | 127, 166, 234, 270 |
| SspI | AATATT | 1 cut(s) | 290 |
| SspMI | CTAG | 1 cut(s) | 65 |
| TaqI | TCGA | 1 cut(s) | 32 |
| TasI | AATT | 4 cut(s) | 127, 166, 234, 270 |
| TatI | WGTACW | 1 cut(s) | 293 |
| TfiI | GAWTC | 1 cut(s) | 92 |
| TseFI | GTSAC | 1 cut(s) | 217 |
| Tsp45I | GTSAC | 1 cut(s) | 217 |
| XmnI | GAANNNNTTC | 1 cut(s) | 177 |
| XspI | CTAG | 1 cut(s) | 65 |