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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 22 |
| AclWI | GGATC | 1 cut(s) | 13 |
| AcuI | CTGAAG | 1 cut(s) | 84 |
| AfeI | AGCGCT | 1 cut(s) | 117 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 252 |
| AgsI | TTSAA | 2 cut(s) | 166, 173 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 114, 146 |
| AluBI | AGCT | 5 cut(s) | 106, 230, 286, 323, 340 |
| AluI | AGCT | 5 cut(s) | 106, 230, 286, 323, 340 |
| AlwI | GGATC | 1 cut(s) | 13 |
| Aor51HI | AGCGCT | 1 cut(s) | 117 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 231 |
| AspLEI | GCGC | 3 cut(s) | 35, 118, 189 |
| BfaI | CTAG | 1 cut(s) | 119 |
| BfmI | CTRYAG | 1 cut(s) | 239 |
| BfoI | RGCGCY | 2 cut(s) | 36, 119 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 30 |
| BmsI | GCATC | 1 cut(s) | 85 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 68, 98 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 252 |
| Bse3DI | GCAATG | 1 cut(s) | 142 |
| BseGI | GGATG | 1 cut(s) | 100 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 252 |
| BseMI | GCAATG | 1 cut(s) | 142 |
| BseRI | GAGGAG | 1 cut(s) | 143 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 252 |
| Bsp143I | GATC | 2 cut(s) | 5, 87 |
| BspACI | CCGC | 1 cut(s) | 22 |
| BspPI | GGATC | 1 cut(s) | 13 |
| BspQI | GCTCTTC | 2 cut(s) | 117, 237 |
| BsrDI | GCAATG | 1 cut(s) | 142 |
| BssMI | GATC | 2 cut(s) | 5, 87 |
| Bst4CI | ACNGT | 3 cut(s) | 44, 56, 243 |
| Bst6I | CTCTTC | 2 cut(s) | 117, 237 |
| BstF5I | GGATG | 1 cut(s) | 100 |
| BstH2I | RGCGCY | 2 cut(s) | 36, 119 |
| BstHHI | GCGC | 3 cut(s) | 35, 118, 189 |
| BstKTI | GATC | 2 cut(s) | 8, 90 |
| BstMBI | GATC | 2 cut(s) | 5, 87 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 30, 124 |
| BstSFI | CTRYAG | 1 cut(s) | 239 |
| BtsCI | GGATG | 1 cut(s) | 100 |
| CfoI | GCGC | 3 cut(s) | 35, 118, 189 |
| CviAII | CATG | 1 cut(s) | 247 |
| CviJI | RGCY | 7 cut(s) | 16, 68, 106, 230, 286, 323, 340 |
| CviKI_1 | RGCY | 7 cut(s) | 16, 68, 106, 230, 286, 323, 340 |
| DpnI | GATC | 2 cut(s) | 7, 89 |
| DpnII | GATC | 2 cut(s) | 5, 87 |
| Eam1104I | CTCTTC | 2 cut(s) | 117, 237 |
| EarI | CTCTTC | 2 cut(s) | 117, 237 |
| EciI | GGCGGA | 1 cut(s) | 11 |
| Eco32I | GATATC | 1 cut(s) | 199 |
| Eco47III | AGCGCT | 1 cut(s) | 117 |
| Eco57I | CTGAAG | 1 cut(s) | 84 |
| EcoRV | GATATC | 1 cut(s) | 199 |
| FaeI | CATG | 1 cut(s) | 250 |
| FaiI | YATR | 5 cut(s) | 81, 83, 109, 219, 248 |
| FatI | CATG | 1 cut(s) | 246 |
| FokI | GGATG | 1 cut(s) | 107 |
| FspBI | CTAG | 1 cut(s) | 119 |
| GlaI | GCGC | 3 cut(s) | 34, 117, 188 |
| HaeII | RGCGCY | 2 cut(s) | 36, 119 |
| HhaI | GCGC | 3 cut(s) | 35, 118, 189 |
| Hin1II | CATG | 1 cut(s) | 250 |
| Hin6I | GCGC | 3 cut(s) | 33, 116, 187 |
| HinP1I | GCGC | 3 cut(s) | 33, 116, 187 |
| HinfI | GANTC | 1 cut(s) | 146 |
| Hpy166II | GTNNAC | 1 cut(s) | 263 |
| Hpy188I | TCNGA | 1 cut(s) | 226 |
| Hpy8I | GTNNAC | 1 cut(s) | 263 |
| HpyAV | CCTTC | 2 cut(s) | 160, 319 |
| HpyCH4III | ACNGT | 3 cut(s) | 44, 56, 243 |
| HpyCH4V | TGCA | 1 cut(s) | 98 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 30, 124 |
| Hsp92II | CATG | 1 cut(s) | 250 |
| HspAI | GCGC | 3 cut(s) | 33, 116, 187 |
| Kzo9I | GATC | 2 cut(s) | 5, 87 |
| LguI | GCTCTTC | 2 cut(s) | 117, 237 |
| LpnPI | CCDG | 2 cut(s) | 21, 171 |
| LweI | GCATC | 1 cut(s) | 85 |
| MaeI | CTAG | 1 cut(s) | 119 |
| MaeIII | GTNAC | 3 cut(s) | 38, 50, 342 |
| MalI | GATC | 2 cut(s) | 7, 89 |
| MboI | GATC | 2 cut(s) | 5, 87 |
| MboII | GAAGA | 2 cut(s) | 134, 224 |
| MluCI | AATT | 2 cut(s) | 220, 288 |
| MnlI | CCTC | 3 cut(s) | 161, 164, 246 |
| MroXI | GAANNNNTTC | 1 cut(s) | 231 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 30, 124 |
| NdeII | GATC | 2 cut(s) | 5, 87 |
| NlaIII | CATG | 1 cut(s) | 250 |
| NmuCI | GTSAC | 2 cut(s) | 50, 342 |
| PciSI | GCTCTTC | 2 cut(s) | 117, 237 |
| PdmI | GAANNNNTTC | 1 cut(s) | 231 |
| PfeI | GAWTC | 1 cut(s) | 146 |
| SapI | GCTCTTC | 2 cut(s) | 117, 237 |
| Sau3AI | GATC | 2 cut(s) | 5, 87 |
| SetI | ASST | 9 cut(s) | 108, 171, 232, 257, 271, 288, 311, 325, 342 |
| SfaNI | GCATC | 1 cut(s) | 85 |
| SfcI | CTRYAG | 1 cut(s) | 239 |
| Sse9I | AATT | 2 cut(s) | 220, 288 |
| SsiI | CCGC | 1 cut(s) | 22 |
| SspMI | CTAG | 1 cut(s) | 119 |
| TaaI | ACNGT | 3 cut(s) | 44, 56, 243 |
| TaqI | TCGA | 1 cut(s) | 86 |
| TasI | AATT | 2 cut(s) | 220, 288 |
| TfiI | GAWTC | 1 cut(s) | 146 |
| TseFI | GTSAC | 2 cut(s) | 50, 342 |
| Tsp45I | GTSAC | 2 cut(s) | 50, 342 |
| XmnI | GAANNNNTTC | 1 cut(s) | 231 |
| XspI | CTAG | 1 cut(s) | 119 |