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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcyI | GRCGYC | 1 cut(s) | 46 |
| AfeI | AGCGCT | 2 cut(s) | 114, 125 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 249 |
| AgsI | TTSAA | 3 cut(s) | 163, 170, 199 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 266 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 111, 143 |
| AluBI | AGCT | 3 cut(s) | 103, 227, 282 |
| AluI | AGCT | 3 cut(s) | 103, 227, 282 |
| Aor51HI | AGCGCT | 2 cut(s) | 114, 125 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 228 |
| AspLEI | GCGC | 3 cut(s) | 115, 126, 186 |
| BfaI | CTAG | 2 cut(s) | 8, 116 |
| BfoI | RGCGCY | 2 cut(s) | 116, 127 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 266 |
| BmsI | GCATC | 1 cut(s) | 82 |
| BsaHI | GRCGYC | 1 cut(s) | 46 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 65, 95 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 249 |
| Bse3DI | GCAATG | 1 cut(s) | 139 |
| BseGI | GGATG | 1 cut(s) | 97 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 249 |
| BseMI | GCAATG | 1 cut(s) | 139 |
| BseMII | CTCAG | 1 cut(s) | 79 |
| BseRI | GAGGAG | 1 cut(s) | 140 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 249 |
| Bsp143I | GATC | 1 cut(s) | 84 |
| BspCNI | CTCAG | 1 cut(s) | 78 |
| BspQI | GCTCTTC | 2 cut(s) | 114, 234 |
| BsrDI | GCAATG | 1 cut(s) | 139 |
| BssMI | GATC | 1 cut(s) | 84 |
| BssNI | GRCGYC | 1 cut(s) | 46 |
| Bst6I | CTCTTC | 2 cut(s) | 114, 234 |
| BstACI | GRCGYC | 1 cut(s) | 46 |
| BstDEI | CTNAG | 1 cut(s) | 65 |
| BstF5I | GGATG | 1 cut(s) | 97 |
| BstH2I | RGCGCY | 2 cut(s) | 116, 127 |
| BstHHI | GCGC | 3 cut(s) | 115, 126, 186 |
| BstKTI | GATC | 1 cut(s) | 87 |
| BstMBI | GATC | 1 cut(s) | 84 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 121 |
| BstNSI | RCATGY | 1 cut(s) | 238 |
| BtsCI | GGATG | 1 cut(s) | 97 |
| CfoI | GCGC | 3 cut(s) | 115, 126, 186 |
| CseI | GACGC | 1 cut(s) | 54 |
| CviAII | CATG | 3 cut(s) | 24, 235, 244 |
| CviJI | RGCY | 4 cut(s) | 41, 103, 227, 282 |
| CviKI_1 | RGCY | 4 cut(s) | 41, 103, 227, 282 |
| DdeI | CTNAG | 1 cut(s) | 65 |
| DpnI | GATC | 1 cut(s) | 86 |
| DpnII | GATC | 1 cut(s) | 84 |
| DriI | GACNNNNNGTC | 1 cut(s) | 266 |
| Eam1104I | CTCTTC | 2 cut(s) | 114, 234 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 266 |
| EarI | CTCTTC | 2 cut(s) | 114, 234 |
| Eco47III | AGCGCT | 2 cut(s) | 114, 125 |
| FaeI | CATG | 3 cut(s) | 27, 238, 247 |
| FaiI | YATR | 9 cut(s) | 25, 51, 53, 78, 80, 106, 216, 236, 245 |
| FatI | CATG | 3 cut(s) | 23, 234, 243 |
| FauNDI | CATATG | 1 cut(s) | 51 |
| FokI | GGATG | 1 cut(s) | 104 |
| FspBI | CTAG | 2 cut(s) | 8, 116 |
| GlaI | GCGC | 3 cut(s) | 114, 125, 185 |
| HaeII | RGCGCY | 2 cut(s) | 116, 127 |
| HgaI | GACGC | 1 cut(s) | 54 |
| HhaI | GCGC | 3 cut(s) | 115, 126, 186 |
| Hin1I | GRCGYC | 1 cut(s) | 46 |
| Hin1II | CATG | 3 cut(s) | 27, 238, 247 |
| Hin6I | GCGC | 3 cut(s) | 113, 124, 184 |
| HinP1I | GCGC | 3 cut(s) | 113, 124, 184 |
| HinfI | GANTC | 3 cut(s) | 11, 31, 143 |
| Hpy166II | GTNNAC | 1 cut(s) | 260 |
| Hpy188I | TCNGA | 2 cut(s) | 68, 223 |
| Hpy188III | TCNNGA | 1 cut(s) | 271 |
| Hpy8I | GTNNAC | 1 cut(s) | 260 |
| Hpy99I | CGWCG | 1 cut(s) | 48 |
| HpyAV | CCTTC | 1 cut(s) | 157 |
| HpyCH4V | TGCA | 2 cut(s) | 95, 238 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 121 |
| HpyF3I | CTNAG | 1 cut(s) | 65 |
| Hsp92I | GRCGYC | 1 cut(s) | 46 |
| Hsp92II | CATG | 3 cut(s) | 27, 238, 247 |
| HspAI | GCGC | 3 cut(s) | 113, 124, 184 |
| Kzo9I | GATC | 1 cut(s) | 84 |
| LguI | GCTCTTC | 2 cut(s) | 114, 234 |
| LpnPI | CCDG | 3 cut(s) | 23, 168, 279 |
| LweI | GCATC | 1 cut(s) | 82 |
| MaeI | CTAG | 2 cut(s) | 8, 116 |
| MalI | GATC | 1 cut(s) | 86 |
| MboI | GATC | 1 cut(s) | 84 |
| MboII | GAAGA | 2 cut(s) | 131, 221 |
| MluCI | AATT | 1 cut(s) | 217 |
| MlyI | GAGTC | 1 cut(s) | 25 |
| MnlI | CCTC | 3 cut(s) | 158, 161, 243 |
| MroXI | GAANNNNTTC | 1 cut(s) | 228 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 121 |
| NdeI | CATATG | 1 cut(s) | 51 |
| NdeII | GATC | 1 cut(s) | 84 |
| NlaIII | CATG | 3 cut(s) | 27, 238, 247 |
| NspI | RCATGY | 1 cut(s) | 238 |
| PciSI | GCTCTTC | 2 cut(s) | 114, 234 |
| PdmI | GAANNNNTTC | 1 cut(s) | 228 |
| PfeI | GAWTC | 2 cut(s) | 11, 143 |
| PleI | GAGTC | 1 cut(s) | 25 |
| PpsI | GAGTC | 1 cut(s) | 25 |
| SapI | GCTCTTC | 2 cut(s) | 114, 234 |
| Sau3AI | GATC | 1 cut(s) | 84 |
| SchI | GAGTC | 1 cut(s) | 25 |
| SetI | ASST | 7 cut(s) | 9, 105, 168, 229, 254, 268, 284 |
| SfaNI | GCATC | 1 cut(s) | 82 |
| Sse9I | AATT | 1 cut(s) | 217 |
| SspMI | CTAG | 2 cut(s) | 8, 116 |
| TaqI | TCGA | 3 cut(s) | 14, 43, 83 |
| TasI | AATT | 1 cut(s) | 217 |
| TfiI | GAWTC | 2 cut(s) | 11, 143 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| XceI | RCATGY | 1 cut(s) | 238 |
| XmnI | GAANNNNTTC | 1 cut(s) | 228 |
| XspI | CTAG | 2 cut(s) | 8, 116 |