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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 156 |
| AcyI | GRCGYC | 1 cut(s) | 13 |
| AfeI | AGCGCT | 2 cut(s) | 81, 92 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 216 |
| AgsI | TTSAA | 3 cut(s) | 130, 137, 166 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 78, 110 |
| AluBI | AGCT | 3 cut(s) | 70, 194, 250 |
| AluI | AGCT | 3 cut(s) | 70, 194, 250 |
| Aor51HI | AGCGCT | 2 cut(s) | 81, 92 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 195 |
| AspLEI | GCGC | 4 cut(s) | 82, 93, 153, 311 |
| BfaI | CTAG | 1 cut(s) | 83 |
| BfoI | RGCGCY | 2 cut(s) | 83, 94 |
| BfuAI | ACCTGC | 1 cut(s) | 156 |
| BsaHI | GRCGYC | 1 cut(s) | 13 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 32, 62 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 216 |
| Bse3DI | GCAATG | 2 cut(s) | 106, 288 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 216 |
| BseMI | GCAATG | 2 cut(s) | 106, 288 |
| BseMII | CTCAG | 1 cut(s) | 46 |
| BseRI | GAGGAG | 1 cut(s) | 107 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 216 |
| BsmI | GAATGC | 1 cut(s) | 64 |
| BspCNI | CTCAG | 1 cut(s) | 45 |
| BspMI | ACCTGC | 1 cut(s) | 156 |
| BspQI | GCTCTTC | 2 cut(s) | 81, 201 |
| BsrDI | GCAATG | 2 cut(s) | 106, 288 |
| BssNI | GRCGYC | 1 cut(s) | 13 |
| Bst4CI | ACNGT | 1 cut(s) | 207 |
| Bst6I | CTCTTC | 2 cut(s) | 81, 201 |
| BstACI | GRCGYC | 1 cut(s) | 13 |
| BstDEI | CTNAG | 1 cut(s) | 32 |
| BstH2I | RGCGCY | 2 cut(s) | 83, 94 |
| BstHHI | GCGC | 4 cut(s) | 82, 93, 153, 311 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 88 |
| BveI | ACCTGC | 1 cut(s) | 156 |
| CfoI | GCGC | 4 cut(s) | 82, 93, 153, 311 |
| CseI | GACGC | 1 cut(s) | 21 |
| CviAII | CATG | 1 cut(s) | 211 |
| CviJI | RGCY | 4 cut(s) | 70, 194, 250, 287 |
| CviKI_1 | RGCY | 4 cut(s) | 70, 194, 250, 287 |
| DdeI | CTNAG | 1 cut(s) | 32 |
| Eam1104I | CTCTTC | 2 cut(s) | 81, 201 |
| EarI | CTCTTC | 2 cut(s) | 81, 201 |
| Eco47III | AGCGCT | 2 cut(s) | 81, 92 |
| FaeI | CATG | 1 cut(s) | 214 |
| FaiI | YATR | 9 cut(s) | 18, 20, 45, 47, 73, 183, 203, 212, 343 |
| FatI | CATG | 1 cut(s) | 210 |
| FauNDI | CATATG | 1 cut(s) | 18 |
| FspBI | CTAG | 1 cut(s) | 83 |
| GlaI | GCGC | 4 cut(s) | 81, 92, 152, 310 |
| HaeII | RGCGCY | 2 cut(s) | 83, 94 |
| HgaI | GACGC | 1 cut(s) | 21 |
| HhaI | GCGC | 4 cut(s) | 82, 93, 153, 311 |
| Hin1I | GRCGYC | 1 cut(s) | 13 |
| Hin1II | CATG | 1 cut(s) | 214 |
| Hin6I | GCGC | 4 cut(s) | 80, 91, 151, 309 |
| HinP1I | GCGC | 4 cut(s) | 80, 91, 151, 309 |
| HinfI | GANTC | 1 cut(s) | 110 |
| Hpy166II | GTNNAC | 1 cut(s) | 227 |
| Hpy188I | TCNGA | 2 cut(s) | 35, 190 |
| Hpy8I | GTNNAC | 1 cut(s) | 227 |
| Hpy99I | CGWCG | 1 cut(s) | 15 |
| HpyAV | CCTTC | 2 cut(s) | 124, 283 |
| HpyCH4III | ACNGT | 1 cut(s) | 207 |
| HpyCH4V | TGCA | 1 cut(s) | 62 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 88 |
| HpyF3I | CTNAG | 1 cut(s) | 32 |
| Hsp92I | GRCGYC | 1 cut(s) | 13 |
| Hsp92II | CATG | 1 cut(s) | 214 |
| HspAI | GCGC | 4 cut(s) | 80, 91, 151, 309 |
| LguI | GCTCTTC | 2 cut(s) | 81, 201 |
| LmnI | GCTCC | 1 cut(s) | 306 |
| LpnPI | CCDG | 5 cut(s) | 135, 161, 246, 291, 336 |
| MaeI | CTAG | 1 cut(s) | 83 |
| MaeIII | GTNAC | 1 cut(s) | 235 |
| MboII | GAAGA | 2 cut(s) | 98, 188 |
| MluCI | AATT | 3 cut(s) | 184, 252, 314 |
| MnlI | CCTC | 3 cut(s) | 125, 128, 210 |
| MroXI | GAANNNNTTC | 1 cut(s) | 195 |
| MseI | TTAA | 2 cut(s) | 54, 318 |
| Mva1269I | GAATGC | 1 cut(s) | 64 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 88 |
| NdeI | CATATG | 1 cut(s) | 18 |
| NlaIII | CATG | 1 cut(s) | 214 |
| NmuCI | GTSAC | 1 cut(s) | 235 |
| PciSI | GCTCTTC | 2 cut(s) | 81, 201 |
| PctI | GAATGC | 1 cut(s) | 64 |
| PdmI | GAANNNNTTC | 1 cut(s) | 195 |
| PfeI | GAWTC | 1 cut(s) | 110 |
| SapI | GCTCTTC | 2 cut(s) | 81, 201 |
| SaqAI | TTAA | 2 cut(s) | 54, 318 |
| SetI | ASST | 8 cut(s) | 72, 135, 150, 196, 221, 235, 252, 275 |
| Sse9I | AATT | 3 cut(s) | 184, 252, 314 |
| SspMI | CTAG | 1 cut(s) | 83 |
| TaaI | ACNGT | 1 cut(s) | 207 |
| TaqI | TCGA | 1 cut(s) | 50 |
| TasI | AATT | 3 cut(s) | 184, 252, 314 |
| TfiI | GAWTC | 1 cut(s) | 110 |
| Tru1I | TTAA | 2 cut(s) | 54, 318 |
| Tru9I | TTAA | 2 cut(s) | 54, 318 |
| TseFI | GTSAC | 1 cut(s) | 235 |
| Tsp45I | GTSAC | 1 cut(s) | 235 |
| XmnI | GAANNNNTTC | 1 cut(s) | 195 |
| XspI | CTAG | 1 cut(s) | 83 |