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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 1 cut(s) | 150 |
| AcyI | GRCGYC | 1 cut(s) | 7 |
| AfaI | GTAC | 1 cut(s) | 38 |
| AfeI | AGCGCT | 1 cut(s) | 86 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 210 |
| AflIII | ACRYGT | 1 cut(s) | 39 |
| AgsI | TTSAA | 4 cut(s) | 101, 124, 131, 160 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 160 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 72, 104 |
| AluBI | AGCT | 2 cut(s) | 74, 188 |
| AluI | AGCT | 2 cut(s) | 74, 188 |
| Alw26I | GTCTC | 1 cut(s) | 54 |
| Aor51HI | AGCGCT | 1 cut(s) | 86 |
| AoxI | GGCC | 1 cut(s) | 150 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 189 |
| AspLEI | GCGC | 1 cut(s) | 87 |
| BauI | CACGAG | 1 cut(s) | 57 |
| BccI | CCATC | 1 cut(s) | 116 |
| BcoDI | GTCTC | 1 cut(s) | 54 |
| BfmI | CTRYAG | 1 cut(s) | 75 |
| BfoI | RGCGCY | 1 cut(s) | 88 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 160 |
| BmsI | GCATC | 1 cut(s) | 43 |
| BsaHI | GRCGYC | 1 cut(s) | 7 |
| BsaI | GGTCTC | 1 cut(s) | 54 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 26, 56 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 210 |
| BseGI | GGATG | 2 cut(s) | 58, 108 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 210 |
| BseMII | CTCAG | 1 cut(s) | 40 |
| BshFI | GGCC | 1 cut(s) | 152 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 210 |
| BsmAI | GTCTC | 1 cut(s) | 54 |
| BsnI | GGCC | 1 cut(s) | 152 |
| Bso31I | GGTCTC | 1 cut(s) | 54 |
| Bsp143I | GATC | 1 cut(s) | 45 |
| BspANI | GGCC | 1 cut(s) | 152 |
| BspCNI | CTCAG | 1 cut(s) | 39 |
| BspQI | GCTCTTC | 2 cut(s) | 75, 195 |
| BspTNI | GGTCTC | 1 cut(s) | 54 |
| BssMI | GATC | 1 cut(s) | 45 |
| BssNI | GRCGYC | 1 cut(s) | 7 |
| BssSI | CACGAG | 1 cut(s) | 57 |
| Bst2BI | CACGAG | 1 cut(s) | 57 |
| Bst6I | CTCTTC | 2 cut(s) | 75, 195 |
| BstACI | GRCGYC | 1 cut(s) | 7 |
| BstDEI | CTNAG | 1 cut(s) | 26 |
| BstF5I | GGATG | 2 cut(s) | 58, 108 |
| BstH2I | RGCGCY | 1 cut(s) | 88 |
| BstHHI | GCGC | 1 cut(s) | 87 |
| BstKTI | GATC | 1 cut(s) | 48 |
| BstMAI | GTCTC | 1 cut(s) | 54 |
| BstMBI | GATC | 1 cut(s) | 45 |
| BstNSI | RCATGY | 1 cut(s) | 43 |
| BstSFI | CTRYAG | 1 cut(s) | 75 |
| BsuRI | GGCC | 1 cut(s) | 152 |
| BtsCI | GGATG | 2 cut(s) | 58, 108 |
| CfoI | GCGC | 1 cut(s) | 87 |
| CseI | GACGC | 1 cut(s) | 15 |
| Csp6I | GTAC | 1 cut(s) | 37 |
| CviAII | CATG | 3 cut(s) | 13, 40, 205 |
| CviJI | RGCY | 3 cut(s) | 74, 152, 188 |
| CviKI_1 | RGCY | 3 cut(s) | 74, 152, 188 |
| CviQI | GTAC | 1 cut(s) | 37 |
| DdeI | CTNAG | 1 cut(s) | 26 |
| DpnI | GATC | 1 cut(s) | 47 |
| DpnII | GATC | 1 cut(s) | 45 |
| DriI | GACNNNNNGTC | 1 cut(s) | 160 |
| EaeI | YGGCCR | 1 cut(s) | 150 |
| Eam1104I | CTCTTC | 2 cut(s) | 75, 195 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 160 |
| EarI | CTCTTC | 2 cut(s) | 75, 195 |
| Eco31I | GGTCTC | 1 cut(s) | 54 |
| Eco47III | AGCGCT | 1 cut(s) | 86 |
| FaeI | CATG | 3 cut(s) | 16, 43, 208 |
| FaiI | YATR | 6 cut(s) | 14, 41, 67, 143, 177, 206 |
| FatI | CATG | 3 cut(s) | 12, 39, 204 |
| FokI | GGATG | 2 cut(s) | 65, 95 |
| GlaI | GCGC | 1 cut(s) | 86 |
| HaeII | RGCGCY | 1 cut(s) | 88 |
| HaeIII | GGCC | 1 cut(s) | 152 |
| HgaI | GACGC | 1 cut(s) | 15 |
| HhaI | GCGC | 1 cut(s) | 87 |
| Hin1I | GRCGYC | 1 cut(s) | 7 |
| Hin1II | CATG | 3 cut(s) | 16, 43, 208 |
| Hin6I | GCGC | 1 cut(s) | 85 |
| HinP1I | GCGC | 1 cut(s) | 85 |
| HinfI | GANTC | 1 cut(s) | 104 |
| Hpy166II | GTNNAC | 1 cut(s) | 221 |
| Hpy188I | TCNGA | 2 cut(s) | 29, 184 |
| Hpy188III | TCNNGA | 1 cut(s) | 101 |
| Hpy8I | GTNNAC | 1 cut(s) | 221 |
| Hpy99I | CGWCG | 1 cut(s) | 9 |
| HpyAV | CCTTC | 1 cut(s) | 118 |
| HpyCH4V | TGCA | 1 cut(s) | 56 |
| HpyF3I | CTNAG | 1 cut(s) | 26 |
| Hsp92I | GRCGYC | 1 cut(s) | 7 |
| Hsp92II | CATG | 3 cut(s) | 16, 43, 208 |
| HspAI | GCGC | 1 cut(s) | 85 |
| Kzo9I | GATC | 1 cut(s) | 45 |
| LguI | GCTCTTC | 2 cut(s) | 75, 195 |
| LpnPI | CCDG | 1 cut(s) | 129 |
| LweI | GCATC | 1 cut(s) | 43 |
| MalI | GATC | 1 cut(s) | 47 |
| MboI | GATC | 1 cut(s) | 45 |
| MboII | GAAGA | 2 cut(s) | 92, 182 |
| MluCI | AATT | 1 cut(s) | 178 |
| MnlI | CCTC | 4 cut(s) | 122, 204, 206, 237 |
| MroXI | GAANNNNTTC | 1 cut(s) | 189 |
| NdeII | GATC | 1 cut(s) | 45 |
| NlaIII | CATG | 3 cut(s) | 16, 43, 208 |
| NspI | RCATGY | 1 cut(s) | 43 |
| PciI | ACATGT | 1 cut(s) | 39 |
| PciSI | GCTCTTC | 2 cut(s) | 75, 195 |
| PdmI | GAANNNNTTC | 1 cut(s) | 189 |
| PfeI | GAWTC | 1 cut(s) | 104 |
| PscI | ACATGT | 1 cut(s) | 39 |
| RsaI | GTAC | 1 cut(s) | 38 |
| RsaNI | GTAC | 1 cut(s) | 37 |
| SapI | GCTCTTC | 2 cut(s) | 75, 195 |
| Sau3AI | GATC | 1 cut(s) | 45 |
| SetI | ASST | 6 cut(s) | 66, 76, 129, 190, 215, 229 |
| SfaNI | GCATC | 1 cut(s) | 43 |
| SfcI | CTRYAG | 1 cut(s) | 75 |
| Sse9I | AATT | 1 cut(s) | 178 |
| SspI | AATATT | 1 cut(s) | 97 |
| TaqI | TCGA | 1 cut(s) | 44 |
| TasI | AATT | 1 cut(s) | 178 |
| TatI | WGTACW | 1 cut(s) | 36 |
| TfiI | GAWTC | 1 cut(s) | 104 |
| TspGWI | ACGGA | 1 cut(s) | 154 |
| XceI | RCATGY | 1 cut(s) | 43 |
| XmnI | GAANNNNTTC | 1 cut(s) | 189 |