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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 1 cut(s) | 137 |
| AfeI | AGCGCT | 2 cut(s) | 36, 47 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 171 |
| AgsI | TTSAA | 2 cut(s) | 85, 121 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 121 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 33, 65 |
| AluBI | AGCT | 2 cut(s) | 149, 205 |
| AluI | AGCT | 2 cut(s) | 149, 205 |
| Alw26I | GTCTC | 2 cut(s) | 161, 241 |
| Aor51HI | AGCGCT | 2 cut(s) | 36, 47 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 150 |
| AspLEI | GCGC | 2 cut(s) | 37, 48 |
| BbsI | GAAGAC | 1 cut(s) | 221 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 260 |
| BcoDI | GTCTC | 2 cut(s) | 161, 241 |
| BfaI | CTAG | 1 cut(s) | 38 |
| BfoI | RGCGCY | 2 cut(s) | 38, 49 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 121 |
| BmsI | GCATC | 1 cut(s) | 4 |
| BpiI | GAAGAC | 1 cut(s) | 221 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 125, 157 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 171 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 171 |
| BseMII | CTCAG | 2 cut(s) | 123, 252 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 171 |
| BsmAI | GTCTC | 2 cut(s) | 161, 241 |
| BsmBI | CGTCTC | 2 cut(s) | 161, 241 |
| Bsp143I | GATC | 1 cut(s) | 6 |
| BspCNI | CTCAG | 2 cut(s) | 124, 251 |
| BspQI | GCTCTTC | 2 cut(s) | 36, 156 |
| BssMI | GATC | 1 cut(s) | 6 |
| Bst6I | CTCTTC | 2 cut(s) | 36, 156 |
| BstDEI | CTNAG | 2 cut(s) | 132, 238 |
| BstH2I | RGCGCY | 2 cut(s) | 38, 49 |
| BstHHI | GCGC | 2 cut(s) | 37, 48 |
| BstKTI | GATC | 1 cut(s) | 9 |
| BstMAI | GTCTC | 2 cut(s) | 161, 241 |
| BstMBI | GATC | 1 cut(s) | 6 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 43 |
| BstV2I | GAAGAC | 1 cut(s) | 221 |
| BtsIMutI | CAGTG | 1 cut(s) | 98 |
| CfoI | GCGC | 2 cut(s) | 37, 48 |
| Csp6I | GTAC | 1 cut(s) | 136 |
| CviAII | CATG | 1 cut(s) | 166 |
| CviJI | RGCY | 4 cut(s) | 149, 164, 187, 205 |
| CviKI_1 | RGCY | 4 cut(s) | 149, 164, 187, 205 |
| CviQI | GTAC | 1 cut(s) | 136 |
| DdeI | CTNAG | 2 cut(s) | 132, 238 |
| DpnI | GATC | 1 cut(s) | 8 |
| DpnII | GATC | 1 cut(s) | 6 |
| DriI | GACNNNNNGTC | 1 cut(s) | 121 |
| Eam1104I | CTCTTC | 2 cut(s) | 36, 156 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 121 |
| EarI | CTCTTC | 2 cut(s) | 36, 156 |
| Eco32I | GATATC | 1 cut(s) | 252 |
| Eco47III | AGCGCT | 2 cut(s) | 36, 47 |
| EcoRV | GATATC | 1 cut(s) | 252 |
| Esp3I | CGTCTC | 2 cut(s) | 161, 241 |
| FaeI | CATG | 1 cut(s) | 169 |
| FaiI | YATR | 4 cut(s) | 28, 104, 106, 167 |
| FatI | CATG | 1 cut(s) | 165 |
| FauNDI | CATATG | 1 cut(s) | 104 |
| FspBI | CTAG | 1 cut(s) | 38 |
| GlaI | GCGC | 2 cut(s) | 36, 47 |
| HaeII | RGCGCY | 2 cut(s) | 38, 49 |
| HhaI | GCGC | 2 cut(s) | 37, 48 |
| Hin1II | CATG | 1 cut(s) | 169 |
| Hin6I | GCGC | 2 cut(s) | 35, 46 |
| HinP1I | GCGC | 2 cut(s) | 35, 46 |
| HinfI | GANTC | 2 cut(s) | 65, 217 |
| Hpy188I | TCNGA | 2 cut(s) | 115, 145 |
| HpyAV | CCTTC | 1 cut(s) | 79 |
| HpyCH4V | TGCA | 2 cut(s) | 17, 182 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 43 |
| HpyF3I | CTNAG | 2 cut(s) | 132, 238 |
| Hsp92II | CATG | 1 cut(s) | 169 |
| HspAI | GCGC | 2 cut(s) | 35, 46 |
| Kzo9I | GATC | 1 cut(s) | 6 |
| LguI | GCTCTTC | 2 cut(s) | 36, 156 |
| LmnI | GCTCC | 1 cut(s) | 32 |
| LpnPI | CCDG | 2 cut(s) | 86, 90 |
| LweI | GCATC | 1 cut(s) | 4 |
| MaeI | CTAG | 1 cut(s) | 38 |
| MalI | GATC | 1 cut(s) | 8 |
| MboI | GATC | 1 cut(s) | 6 |
| MboII | GAAGA | 4 cut(s) | 53, 143, 209, 226 |
| MfeI | CAATTG | 1 cut(s) | 243 |
| MluCI | AATT | 2 cut(s) | 207, 243 |
| MlyI | GAGTC | 1 cut(s) | 211 |
| MmeI | TCCRAC | 1 cut(s) | 138 |
| MnlI | CCTC | 3 cut(s) | 80, 165, 198 |
| MroXI | GAANNNNTTC | 1 cut(s) | 150 |
| MseI | TTAA | 1 cut(s) | 276 |
| MunI | CAATTG | 1 cut(s) | 243 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 43 |
| NdeI | CATATG | 1 cut(s) | 104 |
| NdeII | GATC | 1 cut(s) | 6 |
| NlaIII | CATG | 1 cut(s) | 169 |
| PciSI | GCTCTTC | 2 cut(s) | 36, 156 |
| PdmI | GAANNNNTTC | 1 cut(s) | 150 |
| PfeI | GAWTC | 1 cut(s) | 65 |
| PleI | GAGTC | 1 cut(s) | 211 |
| PpsI | GAGTC | 1 cut(s) | 211 |
| RsaI | GTAC | 1 cut(s) | 137 |
| RsaNI | GTAC | 1 cut(s) | 136 |
| SapI | GCTCTTC | 2 cut(s) | 36, 156 |
| SaqAI | TTAA | 1 cut(s) | 276 |
| Sau3AI | GATC | 1 cut(s) | 6 |
| SchI | GAGTC | 1 cut(s) | 211 |
| SetI | ASST | 4 cut(s) | 90, 151, 176, 207 |
| SfaNI | GCATC | 1 cut(s) | 4 |
| Sse9I | AATT | 2 cut(s) | 207, 243 |
| SspMI | CTAG | 1 cut(s) | 38 |
| TaqI | TCGA | 1 cut(s) | 5 |
| TasI | AATT | 2 cut(s) | 207, 243 |
| TatI | WGTACW | 1 cut(s) | 135 |
| TfiI | GAWTC | 1 cut(s) | 65 |
| Tru1I | TTAA | 1 cut(s) | 276 |
| Tru9I | TTAA | 1 cut(s) | 276 |
| TscAI | CASTG | 1 cut(s) | 98 |
| TspRI | CASTG | 1 cut(s) | 98 |
| XmnI | GAANNNNTTC | 1 cut(s) | 150 |
| XspI | CTAG | 1 cut(s) | 38 |