Rmu_sc0004655.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004655.1
OG0014875
Physical Location & Seq
Forward (+)
14825 .. 17095
2271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004655.1_g000004.1.cds

Sequence Viewer

Length: 501 bp
atgaaagctactgttcgtaggaaaaagaatcaaatcttacgcattagggatgatctcaataattggaccaatgatcaagatcatattgcttcgatccttatcattgcttttcaaaaacgttttacgtcttctaccactccttctcatgcgttagcatcttttctcttatttatccaaccttgcatttctgagcatgataatgtctcgttattagctccggtctcattggatgatgttattaaggcggttcatagtattggagctttgaaagccccgggtccggatgttaggtgtatgtatctctccgaaccaaggtacctctgcccccgattggagtatatgtcgatcaaggatgcaagagagctatgggtagcgctagaagagcgctttggcaatgtccaagattccctcctccctgacttgaaggttcaatggaacaatctgcgctttgctaatttcaagtctgttgctgaatataattcagaagctcttcgcctatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

166

Amino Acids

19.03

Weight (kDa)

8.46

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 315
AccIII TCCGGA 1 cut(s) 280
AciI CCGC 1 cut(s) 245
AclI AACGTT 1 cut(s) 118
AclWI GGATC 1 cut(s) 88
AfaI GTAC 1 cut(s) 317
AfeI AGCGCT 2 cut(s) 375, 386
AfiI CCNNNNNNNGG 3 cut(s) 280, 312, 331
AgsI TTSAA 5 cut(s) 113, 268, 424, 431, 460
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AluBI AGCT 5 cut(s) 8, 215, 263, 364, 488
AluI AGCT 5 cut(s) 8, 215, 263, 364, 488
Alw26I GTCTC 2 cut(s) 208, 226
AlwI GGATC 1 cut(s) 88
Ama87I CYCGRG 1 cut(s) 274
Aor13HI TCCGGA 1 cut(s) 280
Aor51HI AGCGCT 2 cut(s) 375, 386
Asp700I GAANNNNTTC 1 cut(s) 489
Asp718I GGTACC 1 cut(s) 315
AspLEI GCGC 3 cut(s) 376, 387, 447
AspS9I GGNCC 2 cut(s) 66, 278
AsuC2I CCSGG 2 cut(s) 275, 276
AvaI CYCGRG 1 cut(s) 274
AvaII GGWCC 2 cut(s) 66, 278
BaeI ACNNNNGTAYC 2 cut(s) 299, 332
BanI GGYRCC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 120
BclI TGATCA 1 cut(s) 73
BcnI CCSGG 2 cut(s) 275, 276
BcoDI GTCTC 2 cut(s) 208, 226
BfaI CTAG 1 cut(s) 377
BfmI CTRYAG 1 cut(s) 497
BfoI RGCGCY 2 cut(s) 377, 388
Bme1390I CCNGG 2 cut(s) 275, 276
Bme18I GGWCC 2 cut(s) 66, 278
BmeT110I CYCGRG 1 cut(s) 274
BmgT120I GGNCC 2 cut(s) 66, 278
BmiI GGNNCC 2 cut(s) 279, 317
BmrFI CCNGG 2 cut(s) 275, 276
BmsI GCATC 2 cut(s) 164, 343
BpiI GAAGAC 1 cut(s) 120
BpuMI CCSGG 2 cut(s) 275, 276
BsaBI GATNNNNATC 2 cut(s) 78, 98
BsaI GGTCTC 1 cut(s) 226
BsaJI CCNNGG 3 cut(s) 273, 274, 311
BsaWI WCCGGW 2 cut(s) 217, 280
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 280, 312, 331
Bse3DI GCAATG 2 cut(s) 102, 400
Bse8I GATNNNNATC 2 cut(s) 78, 98
BseAI TCCGGA 1 cut(s) 280
BseDI CCNNGG 3 cut(s) 273, 274, 311
BseGI GGATG 4 cut(s) 55, 235, 289, 358
BseJI GATNNNNATC 2 cut(s) 78, 98
BseLI CCNNNNNNNGG 3 cut(s) 280, 312, 331
BseMI GCAATG 2 cut(s) 102, 400
BseMII CTCAG 1 cut(s) 180
BseRI GAGGAG 1 cut(s) 401
BshNI GGYRCC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 274
BsiSI CCGG 3 cut(s) 218, 275, 281
BslI CCNNNNNNNGG 3 cut(s) 280, 312, 331
BsmAI GTCTC 2 cut(s) 208, 226
Bso31I GGTCTC 1 cut(s) 226
BsoBI CYCGRG 1 cut(s) 274
Bsp13I TCCGGA 1 cut(s) 280
Bsp143I GATC 5 cut(s) 52, 73, 79, 93, 345
BspACI CCGC 1 cut(s) 245
BspCNI CTCAG 1 cut(s) 181
BspEI TCCGGA 1 cut(s) 280
BspLI GGNNCC 2 cut(s) 279, 317
BspPI GGATC 1 cut(s) 88
BspQI GCTCTTC 2 cut(s) 375, 495
BspT107I GGYRCC 1 cut(s) 315
BspTNI GGTCTC 1 cut(s) 226
BsrDI GCAATG 2 cut(s) 102, 400
BssECI CCNNGG 3 cut(s) 273, 274, 311
BssMI GATC 5 cut(s) 52, 73, 79, 93, 345
BssT1I CCWWGG 1 cut(s) 311
Bst4CI ACNGT 1 cut(s) 13
Bst6I CTCTTC 2 cut(s) 375, 495
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 4 cut(s) 55, 235, 289, 358
BstH2I RGCGCY 2 cut(s) 377, 388
BstHHI GCGC 3 cut(s) 376, 387, 447
BstKTI GATC 5 cut(s) 55, 76, 82, 96, 348
BstMAI GTCTC 2 cut(s) 208, 226
BstMBI GATC 5 cut(s) 52, 73, 79, 93, 345
BstMWI GCNNNNNNNGC 2 cut(s) 269, 382
BstSCI CCNGG 2 cut(s) 273, 274
BstSFI CTRYAG 1 cut(s) 497
BstV2I GAAGAC 1 cut(s) 120
BtsCI GGATG 4 cut(s) 55, 235, 289, 358
CfoI GCGC 3 cut(s) 376, 387, 447
Cfr13I GGNCC 2 cut(s) 66, 278
Cfr9I CCCGGG 1 cut(s) 274
Csp6I GTAC 1 cut(s) 316
CviAII CATG 2 cut(s) 146, 194
CviJI RGCY 6 cut(s) 8, 215, 263, 272, 364, 488
CviKI_1 RGCY 6 cut(s) 8, 215, 263, 272, 364, 488
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 1 cut(s) 189
DpnI GATC 5 cut(s) 54, 75, 81, 95, 347
DpnII GATC 5 cut(s) 52, 73, 79, 93, 345
Eam1104I CTCTTC 2 cut(s) 375, 495
EarI CTCTTC 2 cut(s) 375, 495
Eco130I CCWWGG 1 cut(s) 311
Eco31I GGTCTC 1 cut(s) 226
Eco47I GGWCC 2 cut(s) 66, 278
Eco47III AGCGCT 2 cut(s) 375, 386
Eco88I CYCGRG 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 311
FaeI CATG 2 cut(s) 149, 197
FatI CATG 2 cut(s) 145, 193
FbaI TGATCA 1 cut(s) 73
FokI GGATG 4 cut(s) 62, 242, 296, 365
FspBI CTAG 1 cut(s) 377
GlaI GCGC 3 cut(s) 375, 386, 446
HaeII RGCGCY 2 cut(s) 377, 388
HapII CCGG 3 cut(s) 218, 275, 281
HhaI GCGC 3 cut(s) 376, 387, 447
Hin1II CATG 2 cut(s) 149, 197
Hin6I GCGC 3 cut(s) 374, 385, 445
HinP1I GCGC 3 cut(s) 374, 385, 445
HinfI GANTC 2 cut(s) 28, 404
HpaII CCGG 3 cut(s) 218, 275, 281
Hpy188I TCNGA 3 cut(s) 190, 307, 484
Hpy188III TCNNGA 2 cut(s) 77, 281
HpyAV CCTTC 2 cut(s) 150, 418
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 2 cut(s) 118, 125
HpyCH4V TGCA 2 cut(s) 183, 356
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 382
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 2 cut(s) 118, 125
Hsp92II CATG 2 cut(s) 149, 197
HspAI GCGC 3 cut(s) 374, 385, 445
Kpn2I TCCGGA 1 cut(s) 280
KpnI GGTACC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 5 cut(s) 52, 73, 79, 93, 345
LguI GCTCTTC 2 cut(s) 375, 495
LmnI GCTCC 2 cut(s) 220, 260
LpnPI CCDG 4 cut(s) 231, 288, 294, 429
LweI GCATC 2 cut(s) 164, 343
MaeI CTAG 1 cut(s) 377
MaeII ACGT 2 cut(s) 118, 125
MalI GATC 5 cut(s) 54, 75, 81, 95, 347
MboI GATC 5 cut(s) 52, 73, 79, 93, 345
MboII GAAGA 3 cut(s) 120, 392, 482
MluCI AATT 3 cut(s) 61, 454, 478
MmeI TCCRAC 1 cut(s) 199
MnlI CCTC 3 cut(s) 329, 419, 422
MroI TCCGGA 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 489
MseI TTAA 1 cut(s) 240
MslI CAYNNNNRTG 1 cut(s) 198
MspI CCGG 3 cut(s) 218, 275, 281
MspR9I CCNGG 2 cut(s) 275, 276
MwoI GCNNNNNNNGC 2 cut(s) 269, 382
NciI CCSGG 2 cut(s) 275, 276
NdeII GATC 5 cut(s) 52, 73, 79, 93, 345
NlaIII CATG 2 cut(s) 149, 197
NlaIV GGNNCC 2 cut(s) 279, 317
PciSI GCTCTTC 2 cut(s) 375, 495
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 28, 404
Psp1406I AACGTT 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 279, 317
PspPI GGNCC 2 cut(s) 66, 278
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 1 cut(s) 198
SapI GCTCTTC 2 cut(s) 375, 495
SaqAI TTAA 1 cut(s) 240
Sau3AI GATC 5 cut(s) 52, 73, 79, 93, 345
Sau96I GGNCC 2 cut(s) 66, 278
ScrFI CCNGG 2 cut(s) 275, 276
SfaNI GCATC 2 cut(s) 164, 343
SfcI CTRYAG 1 cut(s) 497
SinI GGWCC 2 cut(s) 66, 278
SmaI CCCGGG 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 198
Sse9I AATT 3 cut(s) 61, 454, 478
SsiI CCGC 1 cut(s) 245
SspMI CTAG 1 cut(s) 377
StyD4I CCNGG 2 cut(s) 273, 274
StyI CCWWGG 1 cut(s) 311
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 2 cut(s) 121, 128
TaqI TCGA 2 cut(s) 92, 344
TasI AATT 3 cut(s) 61, 454, 478
TfiI GAWTC 2 cut(s) 28, 404
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TspDTI ATGAA 2 cut(s) 17, 239
TspMI CCCGGG 1 cut(s) 274
VpaK11BI GGWCC 2 cut(s) 66, 278
XmaI CCCGGG 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.