Rmu_co8514483.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8514483.1
OG0010423
Physical Location & Seq
Forward (+)
1 .. 939
939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8514483.1_g000001.1.cds

Sequence Viewer

Length: 575 bp
gtgatcttgaaactgagctgcgtgagatgagattgagtcttgtgatggagatagaaaagcggaagcaagctgaagaatccttggataacttgcgaaaccagtggcagaggattaggcaacagttatctcttgtaggattgactcttcctgcagatcccactgttacaacagagcatgagcagctaggttctgatcctgcagaagagctggggcaacagctttatcttgccaggtttgtatcaaattctattgggagggccatagcaagggctgagatggaggcagagttggaagatcagatcgagtcgaagaactttgagattgctaggttgtgtgacaagcttcataattatgaggctatgaatcaggaaatggttcagaggaaccaggacgtcattgagttggcacggcgtgagagggagaagaaggcaaaacgacaaaggtgggtctggggctcaattgctactgccctcacacttggtacagcagccttggcatactcttattatccatctggaagaggatctccaacctaccattctgagtcgtccgagagtgataatgcaggcaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

190

Amino Acids

21.77

Weight (kDa)

5.17

Isoelectric Point (pI)

56.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 395
AciI CCGC 1 cut(s) 60
AclWI GGATC 3 cut(s) 148, 187, 531
AcsI RAATTY 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 392
AdeI CACNNNGTG 1 cut(s) 412
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 1 cut(s) 266
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 2 cut(s) 229, 386
AjuI GAANNNNNNNTTGG 2 cut(s) 522, 554
AluBI AGCT 6 cut(s) 18, 70, 183, 207, 219, 342
AluI AGCT 6 cut(s) 18, 70, 183, 207, 219, 342
AlwI GGATC 3 cut(s) 148, 187, 531
AoxI GGCC 1 cut(s) 257
ApeKI GCWGC 3 cut(s) 18, 180, 487
ApoI RAATTY 1 cut(s) 243
Asp700I GAANNNNTTC 1 cut(s) 374
AspS9I GGNCC 1 cut(s) 257
BanII GRGCYC 1 cut(s) 457
BbvI GCAGC 3 cut(s) 5, 192, 499
BccI CCATC 3 cut(s) 39, 270, 519
BceAI ACGGC 1 cut(s) 424
BciT130I CCWGG 2 cut(s) 231, 388
BfaI CTAG 2 cut(s) 184, 326
BfmI CTRYAG 2 cut(s) 149, 197
BisI GCNGC 3 cut(s) 19, 181, 488
BlsI GCNGC 3 cut(s) 20, 182, 489
Bme1390I CCNGG 2 cut(s) 231, 388
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 2 cut(s) 231, 388
BsaHI GRCGYC 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 80, 491
BsaXI ACNNNNNCTCC 2 cut(s) 271, 301
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 99
BseBI CCWGG 2 cut(s) 231, 388
BseDI CCNNGG 2 cut(s) 80, 491
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMII CTCAG 3 cut(s) 5, 263, 533
BseNI ACTGG 1 cut(s) 99
BseXI GCAGC 3 cut(s) 5, 192, 499
BseYI CCCAGC 1 cut(s) 207
BshFI GGCC 1 cut(s) 259
BslI CCNNNNNNNGG 1 cut(s) 266
BsnI GGCC 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 457
Bsp143I GATC 6 cut(s) 3, 153, 192, 294, 299, 523
BspACI CCGC 1 cut(s) 60
BspANI GGCC 1 cut(s) 259
BspCNI CTCAG 3 cut(s) 6, 264, 534
BspLI GGNNCC 1 cut(s) 385
BspMAI CTGCAG 2 cut(s) 153, 201
BspPI GGATC 3 cut(s) 148, 187, 531
BspQI GCTCTTC 1 cut(s) 197
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 2 cut(s) 80, 491
BssMI GATC 6 cut(s) 3, 153, 192, 294, 299, 523
BssNI GRCGYC 1 cut(s) 392
BssT1I CCWWGG 2 cut(s) 80, 491
Bst2UI CCWGG 2 cut(s) 231, 388
Bst4CI ACNGT 2 cut(s) 122, 162
Bst6I CTCTTC 3 cut(s) 149, 197, 513
BstACI GRCGYC 1 cut(s) 392
BstC8I GCNNGC 2 cut(s) 68, 567
BstDEI CTNAG 3 cut(s) 14, 272, 542
BstKTI GATC 6 cut(s) 6, 156, 195, 297, 302, 526
BstMBI GATC 6 cut(s) 3, 153, 192, 294, 299, 523
BstMWI GCNNNNNNNGC 2 cut(s) 180, 493
BstNI CCWGG 2 cut(s) 231, 388
BstSCI CCNGG 2 cut(s) 229, 386
BstSFI CTRYAG 2 cut(s) 149, 197
BstV1I GCAGC 3 cut(s) 5, 192, 499
BstX2I RGATCY 2 cut(s) 153, 523
BstYI RGATCY 2 cut(s) 153, 523
BsuRI GGCC 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 106, 158
Cac8I GCNNGC 2 cut(s) 68, 567
Cfr13I GGNCC 1 cut(s) 257
Csp6I GTAC 1 cut(s) 482
CviAII CATG 1 cut(s) 175
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 3 cut(s) 14, 272, 542
DpnI GATC 6 cut(s) 5, 155, 194, 296, 301, 525
DpnII GATC 6 cut(s) 3, 153, 192, 294, 299, 523
DraIII CACNNNGTG 1 cut(s) 412
Eam1104I CTCTTC 3 cut(s) 149, 197, 513
EarI CTCTTC 3 cut(s) 149, 197, 513
Eco130I CCWWGG 2 cut(s) 80, 491
Eco24I GRGCYC 1 cut(s) 457
Eco57I CTGAAG 1 cut(s) 92
EcoRII CCWGG 2 cut(s) 229, 386
EcoT14I CCWWGG 2 cut(s) 80, 491
EcoT38I GRGCYC 1 cut(s) 457
ErhI CCWWGG 2 cut(s) 80, 491
FaeI CATG 1 cut(s) 178
FaiI YATR 6 cut(s) 176, 262, 347, 353, 361, 498
FatI CATG 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 19, 181, 488
FriOI GRGCYC 1 cut(s) 457
Fsp4HI GCNGC 3 cut(s) 19, 181, 488
FspBI CTAG 2 cut(s) 184, 326
GluI GCNGC 3 cut(s) 19, 181, 488
GsaI CCCAGC 1 cut(s) 211
HaeIII GGCC 1 cut(s) 259
Hin1I GRCGYC 1 cut(s) 392
Hin1II CATG 1 cut(s) 178
HindIII AAGCTT 1 cut(s) 340
HinfI GANTC 6 cut(s) 36, 76, 141, 304, 363, 544
Hpy188I TCNGA 5 cut(s) 192, 299, 380, 543, 552
Hpy188III TCNNGA 3 cut(s) 7, 367, 515
HpyAV CCTTC 1 cut(s) 420
HpyCH4III ACNGT 2 cut(s) 122, 162
HpyCH4IV ACGT 1 cut(s) 392
HpyCH4V TGCA 3 cut(s) 151, 199, 565
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 493
HpyF3I CTNAG 3 cut(s) 14, 272, 542
HpySE526I ACGT 1 cut(s) 392
Hsp92I GRCGYC 1 cut(s) 392
Hsp92II CATG 1 cut(s) 178
Kzo9I GATC 6 cut(s) 3, 153, 192, 294, 299, 523
LguI GCTCTTC 1 cut(s) 197
Lsp1109I GCAGC 3 cut(s) 5, 192, 499
MaeI CTAG 2 cut(s) 184, 326
MaeII ACGT 1 cut(s) 392
MaeIII GTNAC 2 cut(s) 162, 334
MalI GATC 6 cut(s) 5, 155, 194, 296, 301, 525
MboI GATC 6 cut(s) 3, 153, 192, 294, 299, 523
MboII GAAGA 7 cut(s) 85, 136, 214, 304, 321, 435, 530
MfeI CAATTG 1 cut(s) 458
MflI RGATCY 2 cut(s) 153, 523
MhlI GDGCHC 1 cut(s) 457
MluCI AATT 3 cut(s) 243, 348, 458
MlyI GAGTC 4 cut(s) 45, 135, 313, 553
MmeI TCCRAC 2 cut(s) 269, 553
MnlI CCTC 8 cut(s) 101, 248, 273, 348, 374, 410, 481, 514
MroXI GAANNNNTTC 1 cut(s) 374
MslI CAYNNNNRTG 1 cut(s) 350
MspR9I CCNGG 2 cut(s) 231, 388
MunI CAATTG 1 cut(s) 458
MvaI CCWGG 2 cut(s) 231, 388
MwoI GCNNNNNNNGC 2 cut(s) 180, 493
NdeII GATC 6 cut(s) 3, 153, 192, 294, 299, 523
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 1 cut(s) 385
NmuCI GTSAC 1 cut(s) 334
PciSI GCTCTTC 1 cut(s) 197
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 2 cut(s) 76, 363
PkrI GCNGC 3 cut(s) 20, 182, 489
PleI GAGTC 4 cut(s) 44, 135, 312, 552
PpsI GAGTC 4 cut(s) 44, 135, 312, 552
Psp6I CCWGG 2 cut(s) 229, 386
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 2 cut(s) 229, 386
PspN4I GGNNCC 1 cut(s) 385
PspPI GGNCC 1 cut(s) 257
PstI CTGCAG 2 cut(s) 153, 201
PsuI RGATCY 2 cut(s) 153, 523
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 197
SatI GCNGC 3 cut(s) 19, 181, 488
Sau3AI GATC 6 cut(s) 3, 153, 192, 294, 299, 523
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 4 cut(s) 45, 135, 313, 553
ScrFI CCNGG 2 cut(s) 231, 388
SduI GDGCHC 1 cut(s) 457
SfcI CTRYAG 2 cut(s) 149, 197
SmiMI CAYNNNNRTG 1 cut(s) 350
Sse9I AATT 3 cut(s) 243, 348, 458
SsiI CCGC 1 cut(s) 60
SspMI CTAG 2 cut(s) 184, 326
StyD4I CCNGG 2 cut(s) 229, 386
StyI CCWWGG 2 cut(s) 80, 491
TaaI ACNGT 2 cut(s) 122, 162
TaiI ACGT 1 cut(s) 395
TaqI TCGA 2 cut(s) 302, 307
TasI AATT 3 cut(s) 243, 348, 458
TfiI GAWTC 2 cut(s) 76, 363
TscAI CASTG 2 cut(s) 106, 165
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 3 cut(s) 18, 180, 487
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 2 cut(s) 334, 376
TspRI CASTG 2 cut(s) 106, 165
XapI RAATTY 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 2 cut(s) 184, 326
ZraI GACGTC 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.