Rorug02G0094700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010423
Physical Location & Seq
Reverse (-)
7754786 .. 7756460
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0094700.1

Sequence Viewer

Length: 576 bp
ATGGCCACTGCCACCCAGATTGTTGAGAAACTGAAACTGAATCCACACCAGGAAGGTGGTTTCTTCTCTGAAACTTTCAGAGACACTTCCATTCTCCTCCCCTTGTGTCAGCTCCCACCAGACTACAAGGTTGATCGCCCTGTGAGTACAGCTATTTACTTCTTGCTGCCAACTGGGGATGTTTCGCGCCTTCATCGGATACCGTGTGCAGAAACATGGCATTTCTACCTGGGAGAACCTCTTACGGTAATGGAGTTGAATGAGGAAGATGGGAAAGTCAAACTAACAAACATTGGACCAAATTTCATTGGAGAGGATCAGGTACCCCAGTACACAGTGCCTCCAAATGTTTGGTTTGGTGCATTTCCTATAAAGGACTTCAACATTTCACCAGATGGGGGGGTGACCAAAGCTGCTCCAAGGGATTCTGAGACCCACTACTCTCTCGTGGGATGCACTTGTGCACCAGCCTTTCAGTTCCAGGATTTCGAGTTGGCAAAACGCTCAGAGCTCATTAAGCACTTCCCTGATAGCGAGCCTCTTATAACATTGCTAACAGATCCTGAAAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

21.4

Weight (kDa)

4.88

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_5 PF06172 6 - 166 1.1e-41 Cupin superfamily (DUF985)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
Acc65I GGTACC 1 cut(s) 322
AccB1I GGYRCC 1 cut(s) 322
AccII CGCG 1 cut(s) 187
AclWI GGATC 2 cut(s) 324, 554
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 301
AfaI GTAC 3 cut(s) 148, 324, 332
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 2 cut(s) 259, 382
AjnI CCWGG 3 cut(s) 48, 228, 480
AluBI AGCT 4 cut(s) 112, 152, 413, 511
AluI AGCT 4 cut(s) 112, 152, 413, 511
Alw21I GWGCWC 2 cut(s) 466, 513
Alw26I GTCTC 2 cut(s) 75, 425
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 2 cut(s) 324, 554
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 2 cut(s) 166, 413
ApoI RAATTY 1 cut(s) 301
Asp718I GGTACC 1 cut(s) 322
AspLEI GCGC 1 cut(s) 189
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 381, 415
AvaII GGWCC 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 466
BaeI ACNNNNGTAYC 2 cut(s) 314, 347
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 322
BanII GRGCYC 1 cut(s) 513
BauI CACGAG 1 cut(s) 446
Bbv12I GWGCWC 2 cut(s) 466, 513
BbvI GCAGC 2 cut(s) 153, 400
BccI CCATC 2 cut(s) 263, 389
BciT130I CCWGG 3 cut(s) 50, 230, 482
BciVI GTATCC 1 cut(s) 192
BcoDI GTCTC 2 cut(s) 75, 425
BfuI GTATCC 1 cut(s) 192
BisI GCNGC 2 cut(s) 167, 414
BlsI GCNGC 2 cut(s) 168, 415
Bme1390I CCNGG 3 cut(s) 50, 230, 482
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 3 cut(s) 50, 230, 482
BmrI ACTGGG 2 cut(s) 183, 322
BmsI GCATC 1 cut(s) 443
BmuI ACTGGG 2 cut(s) 183, 322
BsaI GGTCTC 1 cut(s) 425
BsaJI CCNNGG 2 cut(s) 229, 419
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 2 cut(s) 178, 328
Bse3DI GCAATG 1 cut(s) 548
BseBI CCWGG 3 cut(s) 50, 230, 482
BseDI CCNNGG 2 cut(s) 229, 419
BseGI GGATG 2 cut(s) 184, 458
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMI GCAATG 1 cut(s) 548
BseMII CTCAG 2 cut(s) 420, 519
BseNI ACTGG 2 cut(s) 178, 328
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 2 cut(s) 153, 400
BsgI GTGCAG 1 cut(s) 228
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 322
BsiHKAI GWGCWC 2 cut(s) 466, 513
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 2 cut(s) 75, 425
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 425
Bsp1286I GDGCHC 2 cut(s) 466, 513
Bsp143I GATC 3 cut(s) 133, 316, 559
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 421, 518
BspFNI CGCG 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 2 cut(s) 324, 554
BspT107I GGYRCC 1 cut(s) 322
BspTNI GGTCTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 548
BsrI ACTGG 2 cut(s) 178, 328
BssECI CCNNGG 2 cut(s) 229, 419
BssMI GATC 3 cut(s) 133, 316, 559
BssSI CACGAG 1 cut(s) 446
BssT1I CCWWGG 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 3 cut(s) 50, 230, 482
Bst4CI ACNGT 3 cut(s) 204, 247, 337
BstC8I GCNNGC 1 cut(s) 536
BstDEI CTNAG 2 cut(s) 429, 505
BstEII GGTNACC 1 cut(s) 403
BstF5I GGATG 2 cut(s) 184, 458
BstFNI CGCG 1 cut(s) 187
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 3 cut(s) 136, 319, 562
BstMAI GTCTC 2 cut(s) 75, 425
BstMBI GATC 3 cut(s) 133, 316, 559
BstMWI GCNNNNNNNGC 1 cut(s) 517
BstNI CCWGG 3 cut(s) 50, 230, 482
BstPI GGTNACC 1 cut(s) 403
BstSCI CCNGG 3 cut(s) 48, 228, 480
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 2 cut(s) 153, 400
BstX2I RGATCY 1 cut(s) 559
BstXI CCANNNNNNTGG 2 cut(s) 56, 351
BstYI RGATCY 1 cut(s) 559
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 184, 458
BtsI GCAGTG 1 cut(s) 6
BtsIMutI CAGTG 2 cut(s) 6, 342
Cac8I GCNNGC 1 cut(s) 536
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 296
Csp6I GTAC 3 cut(s) 147, 323, 331
CspCI CAANNNNNGTGG 1 cut(s) 36
CviAII CATG 1 cut(s) 216
CviJI RGCY 7 cut(s) 5, 112, 152, 413, 470, 511, 538
CviKI_1 RGCY 7 cut(s) 5, 112, 152, 413, 470, 511, 538
CviQI GTAC 3 cut(s) 147, 323, 331
DdeI CTNAG 2 cut(s) 429, 505
DpnI GATC 3 cut(s) 135, 318, 561
DpnII GATC 3 cut(s) 133, 316, 559
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 513
Eco31I GGTCTC 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 296
Eco53kI GAGCTC 1 cut(s) 511
Eco91I GGTNACC 1 cut(s) 403
EcoICRI GAGCTC 1 cut(s) 511
EcoO65I GGTNACC 1 cut(s) 403
EcoRII CCWGG 3 cut(s) 48, 228, 480
EcoT14I CCWWGG 1 cut(s) 419
EcoT38I GRGCYC 1 cut(s) 513
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 1 cut(s) 219
FaiI YATR 4 cut(s) 217, 371, 545, 574
FatI CATG 1 cut(s) 215
Fnu4HI GCNGC 2 cut(s) 167, 414
FokI GGATG 2 cut(s) 191, 465
FriOI GRGCYC 1 cut(s) 513
Fsp4HI GCNGC 2 cut(s) 167, 414
GlaI GCGC 1 cut(s) 188
GluI GCNGC 2 cut(s) 167, 414
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 189
Hin1II CATG 1 cut(s) 219
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HinfI GANTC 2 cut(s) 40, 425
HphI GGTGA 2 cut(s) 381, 415
Hpy166II GTNNAC 2 cut(s) 333, 464
Hpy188I TCNGA 5 cut(s) 70, 80, 198, 430, 508
Hpy188III TCNNGA 1 cut(s) 563
Hpy8I GTNNAC 2 cut(s) 333, 464
HpyAV CCTTC 2 cut(s) 47, 200
HpyCH4III ACNGT 3 cut(s) 204, 247, 337
HpyCH4V TGCA 4 cut(s) 209, 362, 456, 464
HpyF10VI GCNNNNNNNGC 1 cut(s) 517
HpyF3I CTNAG 2 cut(s) 429, 505
Hsp92II CATG 1 cut(s) 219
HspAI GCGC 1 cut(s) 187
KpnI GGTACC 1 cut(s) 326
Kzo9I GATC 3 cut(s) 133, 316, 559
LmnI GCTCC 2 cut(s) 117, 421
Lsp1109I GCAGC 2 cut(s) 153, 400
LweI GCATC 1 cut(s) 443
MaeIII GTNAC 1 cut(s) 403
MalI GATC 3 cut(s) 135, 318, 561
MboI GATC 3 cut(s) 133, 316, 559
MboII GAAGA 2 cut(s) 55, 278
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 2 cut(s) 466, 513
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 301
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 6 cut(s) 107, 249, 256, 307, 351, 549
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 516
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 3 cut(s) 50, 230, 482
MvaI CCWGG 3 cut(s) 50, 230, 482
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 1 cut(s) 517
NdeII GATC 3 cut(s) 133, 316, 559
NlaIII CATG 1 cut(s) 219
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 1 cut(s) 403
PfeI GAWTC 2 cut(s) 40, 425
PfoI TCCNGGA 1 cut(s) 480
PkrI GCNGC 2 cut(s) 168, 415
PsiI TTATAA 1 cut(s) 545
Psp124BI GAGCTC 1 cut(s) 513
Psp6I CCWGG 3 cut(s) 48, 228, 480
PspEI GGTNACC 1 cut(s) 403
PspGI CCWGG 3 cut(s) 48, 228, 480
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 3 cut(s) 148, 324, 332
RsaNI GTAC 3 cut(s) 147, 323, 331
SacI GAGCTC 1 cut(s) 513
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 2 cut(s) 167, 414
Sau3AI GATC 3 cut(s) 133, 316, 559
Sau96I GGNCC 1 cut(s) 296
ScrFI CCNGG 3 cut(s) 50, 230, 482
SduI GDGCHC 2 cut(s) 466, 513
SetI ASST 9 cut(s) 58, 114, 132, 154, 231, 241, 324, 415, 513
SfaNI GCATC 1 cut(s) 443
SinI GGWCC 1 cut(s) 296
Sse9I AATT 1 cut(s) 301
SstI GAGCTC 1 cut(s) 513
StyD4I CCNGG 3 cut(s) 48, 228, 480
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 3 cut(s) 204, 247, 337
TaqI TCGA 1 cut(s) 489
TasI AATT 1 cut(s) 301
TatI WGTACW 2 cut(s) 146, 330
TfiI GAWTC 2 cut(s) 40, 425
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 2 cut(s) 13, 342
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 2 cut(s) 166, 413
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 2 cut(s) 182, 295
TspRI CASTG 2 cut(s) 13, 342
VneI GTGCAC 1 cut(s) 462
VpaK11BI GGWCC 1 cut(s) 296
XapI RAATTY 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.