Rmu_sc0000006.1_g000021

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000006.1
OG0014550
Physical Location & Seq
Forward (+)
79009 .. 81098
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1812 bp
atgagtgaattgaaagagatacatgggatgatgatcactacctcagttgataagaatgtaatcccattgagtaggcttattgatttctgtgctaattctgaagctggagacatcagctatgccaagtcggtttttcgccgaattgagcaacctagtgtctatatctggaactcaatgatcagagggtgctcgaatagcgagaacccagttgagtctttgataatgtatagagaaatggtgcataggggtcatgcccctgaccattttacatttccatttgtgctcaaagggtgttcgataatcgttgatgttgaagttgggaaatgtgttcataactgcattgtgaaaactgggtttgagttgaatgtgtatgcctcttccggtttactcaatatgtatgcttgttgtgcagatatggaatctgcacagaaggtgtttgataaaatgcctaaatggaatgtagttgcttggactagtctgatttccgggtttgttgggaacaatcaagctagtgaggctataaaggtgtttaaggacatggaactttgtaatgtcgagcctaatgagattacgatggtcaatgtcttagttgcttgtgctcgaagtagggatatcaatactgggaagtgggttcacagtcgtattcgccaacttgagtttgatccctttggatcgagctttggttttaatgtgattcttgcgactgccattgtagatatgtatgcaaaatgtggccgcttgagaacagcaaggcatctgtttgacaagatgcctgacagaaacttggttgcctggaattccatgatcagtgcctacaatcaatatggccagggtgataaagcgattgatctcttttttgatatgcggattgctgggtttgttccggaccaagctaccattctgggtgtgattggttcttgtgcgcagttgggggctttggcattgggacaaagtcttcattcgtatgtatcaaaaactagcataggcacagatgcttctattggaactgctttagtgaacatgtatacgaaaaatggagatgcaggcagtgcgcaaagcatttttgacaatatgcagaagaaagatgtaatggcgtggactagtatgattgttggtttagctatgcatgggcatgccgtggaagcactgcatacttttagaagaatgcaagaggatgctagtgttattccagaccagataacttatattggggttttatgcgcgtgtagccatgttgggcttgtggaggagggtcagagacactttggctcaatggttgatgtttatggcataaaaccaactgcagagcattacggctgcatggttgatcttctgagtcgagcaggccggtttgaagaggcggtgaaactactggaagaaatgccgattcaacccaatgttgctatatggggagctctcttaaatgggtgtgagatatatgaaaatgtagatctagctgaccaagttagaagatacataacagagttggagcctcatattagtggagttttcgtccttctttccaatatatatgctagggccggcagatggcaagaagtaaagctgactagagaattgatgaaagataggaggattgccaaaacttggatagctaaagaagctaagtgcgctgcagctgccatagcaaagcttgcaaggaggttggtgctcaatgacctgggaaatggccccccaaccgccatggttaatgttttagtgaatgatgtgctcccatgccccctgtttatcactatgatagcattgattttagtcgcagtagacggtgctagggtatttgcatcttccttatga

Protein Analysis

603

Amino Acids

66.61

Weight (kDa)

6.33

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 51 - 98 5.2e-09 PPR repeat family
PPR_2 PF13041 153 - 200 3.9e-10 PPR repeat family
PPR_2 PF13041 261 - 308 4.2e-08 PPR repeat family
PPR PF01535 263 - 292 1.5e-06 PPR repeat
PPR_1 PF12854 430 - 461 5.1e-06 PPR repeat
E_motif PF20431 480 - 534 6.1e-12 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014550)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05240
fragaria_vesca FvH4_6g05570
malus_domestica MD12G1221500.v1.1
prunus_persica Prupe.6G328300_v2.0.a1
pyrus_communis pycom12g20770
rosa_chinensis RchiOBHm_Chr3g0454351
rosa_laevigata RLG00000025451 RLG00000025452 RLG00000025458
rosa_multiflora Rmu_sc0000006.1_g000021 Rmu_sc0007209.1_g000013
rosa_roxburghii Rroxscaffold_165G00437180 Rroxscaffold_6G00425180
rosa_rugosa Rorug02G0654500
rosa_samantha Rh3BG060200 Rh3DG060600
rosa_wichuraiana Rw3G004500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 924, 1053
AccB7I CCANNNNNTGG 1 cut(s) 1605
AccI GTMKAC 2 cut(s) 1025, 1779
AccII CGCG 1 cut(s) 1223
AccIII TCCGGA 1 cut(s) 883
AciI CCGC 4 cut(s) 736, 865, 1361, 1698
AclWI GGATC 2 cut(s) 656, 679
AcoI YGGCCR 2 cut(s) 733, 826
AcsI RAATTY 1 cut(s) 796
AcuI CTGAAG 1 cut(s) 120
AfiI CCNNNNNNNGG 2 cut(s) 1548, 1605
AflIII ACRYGT 1 cut(s) 1020
AgsI TTSAA 5 cut(s) 13, 314, 364, 1355, 1391
AhlI ACTAGT 2 cut(s) 473, 1100
AjnI CCWGG 3 cut(s) 791, 828, 1677
AjuI GAANNNNNNNTTGG 2 cut(s) 882, 914
Alw21I GWGCWC 6 cut(s) 191, 285, 601, 1417, 1671, 1731
Alw26I GTCTC 2 cut(s) 102, 1252
AlwI GGATC 2 cut(s) 656, 679
Aor13HI TCCGGA 1 cut(s) 883
AoxI GGCC 5 cut(s) 733, 826, 1345, 1539, 1687
ApeKI GCWGC 4 cut(s) 1317, 1631, 1634, 1637
ApoI RAATTY 1 cut(s) 796
AspLEI GCGC 4 cut(s) 925, 1054, 1223, 1631
AspS9I GGNCC 3 cut(s) 886, 1539, 1688
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 2 cut(s) 845, 1375
AvaII GGWCC 1 cut(s) 886
BalI TGGCCA 1 cut(s) 828
BanII GRGCYC 1 cut(s) 1417
BbsI GAAGAC 1 cut(s) 947
Bbv12I GWGCWC 6 cut(s) 191, 285, 601, 1417, 1671, 1731
BbvI GCAGC 4 cut(s) 1304, 1618, 1624, 1646
BccI CCATC 2 cut(s) 568, 1542
BceAI ACGGC 2 cut(s) 1121, 1330
BciT130I CCWGG 3 cut(s) 793, 830, 1679
BclI TGATCA 3 cut(s) 33, 177, 804
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 2 cut(s) 102, 1252
BcuI ACTAGT 2 cut(s) 473, 1100
BfmI CTRYAG 2 cut(s) 1302, 1632
BglII AGATCT 1 cut(s) 1450
BisI GCNGC 5 cut(s) 736, 1318, 1632, 1635, 1638
BlsI GCNGC 5 cut(s) 737, 1319, 1633, 1636, 1639
Bme1390I CCNGG 4 cut(s) 487, 793, 830, 1679
Bme18I GGWCC 1 cut(s) 886
BmgT120I GGNCC 3 cut(s) 886, 1539, 1688
BmiI GGNNCC 2 cut(s) 1491, 1690
BmrFI CCNGG 4 cut(s) 487, 793, 830, 1679
BmrI ACTGGG 3 cut(s) 200, 360, 630
BmsI GCATC 6 cut(s) 759, 763, 982, 1030, 1165, 1808
BmuI ACTGGG 3 cut(s) 200, 360, 630
BpiI GAAGAC 1 cut(s) 947
BpmI CTGGAG 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 674, 760
BpuMI CCSGG 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 32
BsaJI CCNNGG 4 cut(s) 829, 1137, 1678, 1701
BsaWI WCCGGW 2 cut(s) 380, 883
BsaXI ACNNNNNCTCC 2 cut(s) 1497, 1527
Bsc4I CCNNNNNNNGG 2 cut(s) 1548, 1605
Bse118I RCCGGY 2 cut(s) 1347, 1541
Bse1I ACTGG 4 cut(s) 206, 355, 625, 1377
Bse8I GATNNNNATC 1 cut(s) 32
BseAI TCCGGA 1 cut(s) 883
BseBI CCWGG 3 cut(s) 793, 830, 1679
BseDI CCNNGG 4 cut(s) 829, 1137, 1678, 1701
BseGI GGATG 2 cut(s) 33, 1180
BseJI GATNNNNATC 1 cut(s) 32
BseLI CCNNNNNNNGG 2 cut(s) 1548, 1605
BseMII CTCAG 2 cut(s) 57, 1325
BseNI ACTGG 4 cut(s) 206, 355, 625, 1377
BseRI GAGGAG 1 cut(s) 1262
BseXI GCAGC 4 cut(s) 1304, 1618, 1624, 1646
BseYI CCCAGC 1 cut(s) 872
BsgI GTGCAG 2 cut(s) 408, 429
Bsh1236I CGCG 1 cut(s) 1223
BshFI GGCC 5 cut(s) 735, 828, 1347, 1541, 1689
BsiHKAI GWGCWC 6 cut(s) 191, 285, 601, 1417, 1671, 1731
BsiSI CCGG 5 cut(s) 381, 486, 884, 1348, 1542
BslFI GGGAC 1 cut(s) 960
BslI CCNNNNNNNGG 2 cut(s) 1548, 1605
BsmAI GTCTC 2 cut(s) 102, 1252
BsmFI GGGAC 1 cut(s) 960
BsmI GAATGC 1 cut(s) 1170
BsnI GGCC 5 cut(s) 735, 828, 1347, 1541, 1689
Bsp1286I GDGCHC 6 cut(s) 191, 285, 601, 1417, 1671, 1731
Bsp13I TCCGGA 1 cut(s) 883
Bsp143I GATC 8 cut(s) 33, 177, 661, 671, 804, 847, 1327, 1450
Bsp19I CCATGG 1 cut(s) 1701
BspACI CCGC 4 cut(s) 736, 865, 1361, 1698
BspANI GGCC 5 cut(s) 735, 828, 1347, 1541, 1689
BspCNI CTCAG 2 cut(s) 56, 1326
BspEI TCCGGA 1 cut(s) 883
BspFNI CGCG 1 cut(s) 1223
BspLI GGNNCC 2 cut(s) 1491, 1690
BspMAI CTGCAG 2 cut(s) 1306, 1636
BspPI GGATC 2 cut(s) 656, 679
BsrFI RCCGGY 2 cut(s) 1347, 1541
BsrI ACTGG 4 cut(s) 206, 355, 625, 1377
BssAI RCCGGY 2 cut(s) 1347, 1541
BssECI CCNNGG 4 cut(s) 829, 1137, 1678, 1701
BssMI GATC 8 cut(s) 33, 177, 661, 671, 804, 847, 1327, 1450
BssNAI GTATAC 1 cut(s) 1026
BssT1I CCWWGG 1 cut(s) 1701
Bst1107I GTATAC 1 cut(s) 1026
Bst2UI CCWGG 3 cut(s) 793, 830, 1679
Bst4CI ACNGT 2 cut(s) 638, 1784
Bst6I CTCTTC 2 cut(s) 382, 1350
BstAPI GCANNNNNTGC 2 cut(s) 1049, 1652
BstC8I GCNNGC 5 cut(s) 1045, 1134, 1345, 1543, 1653
BstDEI CTNAG 4 cut(s) 43, 586, 1334, 1623
BstDSI CCRYGG 2 cut(s) 1137, 1701
BstF5I GGATG 2 cut(s) 33, 1180
BstFNI CGCG 1 cut(s) 1223
BstHHI GCGC 4 cut(s) 925, 1054, 1223, 1631
BstKTI GATC 8 cut(s) 36, 180, 664, 674, 807, 850, 1330, 1453
BstMAI GTCTC 2 cut(s) 102, 1252
BstMBI GATC 8 cut(s) 33, 177, 661, 671, 804, 847, 1327, 1450
BstNI CCWGG 3 cut(s) 793, 830, 1679
BstNSI RCATGY 2 cut(s) 1024, 1136
BstSCI CCNGG 4 cut(s) 485, 791, 828, 1677
BstSFI CTRYAG 2 cut(s) 1302, 1632
BstUI CGCG 1 cut(s) 1223
BstV1I GCAGC 4 cut(s) 1304, 1618, 1624, 1646
BstV2I GAAGAC 1 cut(s) 947
BstX2I RGATCY 1 cut(s) 1450
BstYI RGATCY 1 cut(s) 1450
BstZ17I GTATAC 1 cut(s) 1026
BsuRI GGCC 5 cut(s) 735, 828, 1347, 1541, 1689
BtgI CCRYGG 2 cut(s) 1137, 1701
BtsCI GGATG 2 cut(s) 33, 1180
BtsI GCAGTG 2 cut(s) 1054, 1145
BtsIMutI CAGTG 3 cut(s) 814, 1054, 1145
Cac8I GCNNGC 5 cut(s) 1045, 1134, 1345, 1543, 1653
CfoI GCGC 4 cut(s) 925, 1054, 1223, 1631
Cfr10I RCCGGY 2 cut(s) 1347, 1541
Cfr13I GGNCC 3 cut(s) 886, 1539, 1688
DdeI CTNAG 4 cut(s) 43, 586, 1334, 1623
DpnI GATC 8 cut(s) 35, 179, 663, 673, 806, 849, 1329, 1452
DpnII GATC 8 cut(s) 33, 177, 661, 671, 804, 847, 1327, 1450
EaeI YGGCCR 2 cut(s) 733, 826
Eam1104I CTCTTC 2 cut(s) 382, 1350
EarI CTCTTC 2 cut(s) 382, 1350
Ecl136II GAGCTC 1 cut(s) 1415
Eco130I CCWWGG 1 cut(s) 1701
Eco24I GRGCYC 1 cut(s) 1417
Eco32I GATATC 1 cut(s) 613
Eco47I GGWCC 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 1415
Eco57I CTGAAG 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 1415
EcoRI GAATTC 1 cut(s) 796
EcoRII CCWGG 3 cut(s) 791, 828, 1677
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 1701
EcoT22I ATGCAT 1 cut(s) 1128
EcoT38I GRGCYC 1 cut(s) 1417
ErhI CCWWGG 1 cut(s) 1701
FaqI GGGAC 1 cut(s) 960
FbaI TGATCA 3 cut(s) 33, 177, 804
FblI GTMKAC 2 cut(s) 1025, 1779
Fnu4HI GCNGC 5 cut(s) 736, 1318, 1632, 1635, 1638
FokI GGATG 2 cut(s) 40, 1187
FriOI GRGCYC 1 cut(s) 1417
Fsp4HI GCNGC 5 cut(s) 736, 1318, 1632, 1635, 1638
FspI TGCGCA 2 cut(s) 924, 1053
GlaI GCGC 4 cut(s) 924, 1053, 1222, 1630
GluI GCNGC 5 cut(s) 736, 1318, 1632, 1635, 1638
GsaI CCCAGC 1 cut(s) 876
GsuI CTGGAG 1 cut(s) 126
HaeIII GGCC 5 cut(s) 735, 828, 1347, 1541, 1689
HapII CCGG 5 cut(s) 381, 486, 884, 1348, 1542
HhaI GCGC 4 cut(s) 925, 1054, 1223, 1631
Hin6I GCGC 4 cut(s) 923, 1052, 1221, 1629
HinP1I GCGC 4 cut(s) 923, 1052, 1221, 1629
HindIII AAGCTT 1 cut(s) 1649
HinfI GANTC 5 cut(s) 212, 419, 694, 1336, 1387
HpaII CCGG 5 cut(s) 381, 486, 884, 1348, 1542
HphI GGTGA 2 cut(s) 845, 1375
Hpy166II GTNNAC 6 cut(s) 386, 634, 1018, 1026, 1098, 1780
Hpy188I TCNGA 5 cut(s) 100, 182, 480, 1257, 1335
Hpy188III TCNNGA 3 cut(s) 166, 884, 1190
Hpy8I GTNNAC 6 cut(s) 386, 634, 1018, 1026, 1098, 1780
HpyAV CCTTC 2 cut(s) 424, 1526
HpyCH4III ACNGT 2 cut(s) 638, 1784
HpyF3I CTNAG 4 cut(s) 43, 586, 1334, 1623
HspAI GCGC 4 cut(s) 923, 1052, 1221, 1629
Kpn2I TCCGGA 1 cut(s) 883
KroI GCCGGC 1 cut(s) 1541
KroNI GCCGGC 1 cut(s) 1543
Ksp22I TGATCA 3 cut(s) 33, 177, 804
Kzo9I GATC 8 cut(s) 33, 177, 661, 671, 804, 847, 1327, 1450
LmnI GCTCC 3 cut(s) 1412, 1489, 1734
Lsp1109I GCAGC 4 cut(s) 1304, 1618, 1624, 1646
LweI GCATC 6 cut(s) 759, 763, 982, 1030, 1165, 1808
MalI GATC 8 cut(s) 35, 179, 663, 673, 806, 849, 1329, 1452
MboI GATC 8 cut(s) 33, 177, 661, 671, 804, 847, 1327, 1450
MboII GAAGA 9 cut(s) 369, 947, 1090, 1173, 1322, 1367, 1388, 1482, 1794
MflI RGATCY 1 cut(s) 1450
MhlI GDGCHC 6 cut(s) 191, 285, 601, 1417, 1671, 1731
MlsI TGGCCA 1 cut(s) 828
MluCI AATT 5 cut(s) 8, 94, 141, 796, 1574
MluNI TGGCCA 1 cut(s) 828
MlyI GAGTC 2 cut(s) 221, 1345
MmeI TCCRAC 1 cut(s) 1467
Mox20I TGGCCA 1 cut(s) 828
Mph1103I ATGCAT 1 cut(s) 1128
MroI TCCGGA 1 cut(s) 883
MroNI GCCGGC 1 cut(s) 1541
MscI TGGCCA 1 cut(s) 828
MseI TTAA 4 cut(s) 531, 687, 1421, 1707
MslI CAYNNNNRTG 3 cut(s) 963, 1131, 1500
Msp20I TGGCCA 1 cut(s) 828
MspA1I CMGCKG 1 cut(s) 1637
MspI CCGG 5 cut(s) 381, 486, 884, 1348, 1542
MspR9I CCNGG 4 cut(s) 487, 793, 830, 1679
Mva1269I GAATGC 1 cut(s) 1170
MvaI CCWGG 3 cut(s) 793, 830, 1679
MvnI CGCG 1 cut(s) 1223
NaeI GCCGGC 1 cut(s) 1543
NciI CCSGG 1 cut(s) 487
NcoI CCATGG 1 cut(s) 1701
NdeII GATC 8 cut(s) 33, 177, 661, 671, 804, 847, 1327, 1450
NgoMIV GCCGGC 1 cut(s) 1541
NlaIV GGNNCC 2 cut(s) 1491, 1690
NsbI TGCGCA 2 cut(s) 924, 1053
NsiI ATGCAT 1 cut(s) 1128
NspI RCATGY 2 cut(s) 1024, 1136
PaeI GCATGC 1 cut(s) 1136
PciI ACATGT 1 cut(s) 1020
PctI GAATGC 1 cut(s) 1170
PdiI GCCGGC 1 cut(s) 1543
PfeI GAWTC 3 cut(s) 419, 694, 1387
PflFI GACNNNGTC 1 cut(s) 951
PflMI CCANNNNNTGG 1 cut(s) 1605
PkrI GCNGC 5 cut(s) 737, 1319, 1633, 1636, 1639
PleI GAGTC 2 cut(s) 220, 1344
PpsI GAGTC 2 cut(s) 220, 1344
PscI ACATGT 1 cut(s) 1020
Psp124BI GAGCTC 1 cut(s) 1417
Psp6I CCWGG 3 cut(s) 791, 828, 1677
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 3 cut(s) 791, 828, 1677
PspN4I GGNNCC 2 cut(s) 1491, 1690
PspPI GGNCC 3 cut(s) 886, 1539, 1688
PstI CTGCAG 2 cut(s) 1306, 1636
PsuI RGATCY 1 cut(s) 1450
PsyI GACNNNGTC 1 cut(s) 951
PvuII CAGCTG 1 cut(s) 1637
RseI CAYNNNNRTG 3 cut(s) 963, 1131, 1500
SacI GAGCTC 1 cut(s) 1417
SaqAI TTAA 4 cut(s) 531, 687, 1421, 1707
SatI GCNGC 5 cut(s) 736, 1318, 1632, 1635, 1638
Sau3AI GATC 8 cut(s) 33, 177, 661, 671, 804, 847, 1327, 1450
Sau96I GGNCC 3 cut(s) 886, 1539, 1688
SchI GAGTC 2 cut(s) 221, 1345
ScrFI CCNGG 4 cut(s) 487, 793, 830, 1679
SduI GDGCHC 6 cut(s) 191, 285, 601, 1417, 1671, 1731
SfaNI GCATC 6 cut(s) 759, 763, 982, 1030, 1165, 1808
SfcI CTRYAG 2 cut(s) 1302, 1632
SinI GGWCC 1 cut(s) 886
SmiMI CAYNNNNRTG 3 cut(s) 963, 1131, 1500
SmlI CTYRAG 2 cut(s) 653, 739
SmoI CTYRAG 2 cut(s) 653, 739
SpeI ACTAGT 2 cut(s) 473, 1100
SphI GCATGC 1 cut(s) 1136
Sse9I AATT 5 cut(s) 8, 94, 141, 796, 1574
SsiI CCGC 4 cut(s) 736, 865, 1361, 1698
SstI GAGCTC 1 cut(s) 1417
StyD4I CCNGG 4 cut(s) 485, 791, 828, 1677
StyI CCWWGG 1 cut(s) 1701
TaaI ACNGT 2 cut(s) 638, 1784
TaqI TCGA 6 cut(s) 191, 296, 555, 601, 674, 1339
TasI AATT 5 cut(s) 8, 94, 141, 796, 1574
TauI GCSGC 1 cut(s) 738
TfiI GAWTC 3 cut(s) 419, 694, 1387
Tru1I TTAA 4 cut(s) 531, 687, 1421, 1707
Tru9I TTAA 4 cut(s) 531, 687, 1421, 1707
TscAI CASTG 3 cut(s) 814, 1054, 1152
TseI GCWGC 4 cut(s) 1317, 1631, 1634, 1637
TspDTI ATGAA 4 cut(s) 320, 947, 1455, 1595
TspRI CASTG 3 cut(s) 814, 1054, 1152
Tth111I GACNNNGTC 1 cut(s) 951
Van91I CCANNNNNTGG 1 cut(s) 1605
VpaK11BI GGWCC 1 cut(s) 886
XapI RAATTY 1 cut(s) 796
XceI RCATGY 2 cut(s) 1024, 1136
XmiI GTMKAC 2 cut(s) 1025, 1779
Zsp2I ATGCAT 1 cut(s) 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.